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The map label for this gene is epsD

Identifier: 16080487

GI number: 16080487

Start: 3525250

End: 3526395

Strand: Reverse

Name: epsD

Synonym: BSU34340

Alternate gene names: 16080487

Gene position: 3526395-3525250 (Counterclockwise)

Preceding gene: 16080488

Following gene: 255767770

Centisome position: 83.65

GC content: 49.48

Gene sequence:

>1146_bases
ATGACGAAAAAGATATTGTTTTGCGCGACTGTTGATTATCATTTTAAGGCCTTTCACCTCCCTTATTTTAAATGGTTCAA
GCAAATGGGCTGGGAGGTTCATGTCGCCGCGAACGGACAAACCAAGCTGCCGTATGTGGATGAGAAATTCTCCATCCCGA
TTCGCAGGTCACCTTTTGACCCTCAGAACCTGGCCGTTTATAGGCAGCTGAAGAAAGTGATTGACACTTATGAATACGAC
ATTGTCCATTGCCATACACCGGTCGGCGGCGTTCTCGCCAGACTGGCGGCGAGGCAGGCACGGCGGCACGGAACAAAGGT
GCTGTACACAGCGCACGGATTTCACTTCTGCAAAGGGGCACCGATGAAAAATTGGCTTCTTTACTATCCGGTTGAGAAAT
GGCTTTCAGCATATACAGACTGCCTGATTACGATTAATGAAGAGGATTACATACGGGCGAAAGGACTTCAAAGGCCGGGC
GGAAGGACGCAGAAAATTCACGGCATTGGCGTCAATACCGAGCGTTTCCGGCCTGTCAGTCCGATAGAGCAGCAAAGACT
CAGAGAAAAGCACGGGTTCCGTGAAGATGATTTTATATTGGTTTATCCGGCTGAGCTCAATCTGAACAAAAACCAGAAGC
AGTTAATTGAAGCCGCAGCCTTGCTAAAAGAAAAAATTCCCTCACTCCGCCTTGTGTTTGCCGGGGAAGGGGCAATGGAA
CATACGTATCAAACGTTAGCTGAAAAGCTTGGTGCCTCCGCCCATGTCTGTTTTTACGGCTTTTGCAGCGACATACATGA
GTTGATTCAGCTTGCGGATGTATCTGTCGCATCCAGCATTAGAGAAGGCCTCGGTATGAATGTGCTTGAGGGAATGGCGG
CAGAACAACCGGCGATCGCCACAGATAATCGCGGGCATCGGGAAATCATCCGCGACGGAGAAAACGGTTTTCTGATCAAA
ATCGGTGACAGTGCTGCTTTTGCCCGCCGGATTGAACAGCTTTACCATAAGCCGGAGCTCTGCCGAAAGCTGGGACAGGA
AGGCCGAAAAACAGCCTTGCGCTTCTCGGAGGCGCGAACGGTGGAAGAAATGGCAGATATTTATTCCGCGTACATGGATA
TGGATACAAAGGAGAAAAGCGTATGA

Upstream 100 bases:

>100_bases
TTCATGAGCTGCCGGAAGCCGACCTGAGAGCGAGGCTGTTTGCGGCAATCAATACATCAGAAGAAATGACGGCTGCCAGC
GTTCATTAGGGGGAGTGAAA

Downstream 100 bases:

>100_bases
ACTCAGGACCGAAAGTTTCTGTCATTATGGGCATTTATAATTGCGAACGCACTTTGGCAGAAAGCATAGAATCCATACTC
AGCCAATCCTATAAAAATTG

Product: extracellular matrix biosynthesis enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MTKKILFCATVDYHFKAFHLPYFKWFKQMGWEVHVAANGQTKLPYVDEKFSIPIRRSPFDPQNLAVYRQLKKVIDTYEYD
IVHCHTPVGGVLARLAARQARRHGTKVLYTAHGFHFCKGAPMKNWLLYYPVEKWLSAYTDCLITINEEDYIRAKGLQRPG
GRTQKIHGIGVNTERFRPVSPIEQQRLREKHGFREDDFILVYPAELNLNKNQKQLIEAAALLKEKIPSLRLVFAGEGAME
HTYQTLAEKLGASAHVCFYGFCSDIHELIQLADVSVASSIREGLGMNVLEGMAAEQPAIATDNRGHREIIRDGENGFLIK
IGDSAAFARRIEQLYHKPELCRKLGQEGRKTALRFSEARTVEEMADIYSAYMDMDTKEKSV

Sequences:

>Translated_381_residues
MTKKILFCATVDYHFKAFHLPYFKWFKQMGWEVHVAANGQTKLPYVDEKFSIPIRRSPFDPQNLAVYRQLKKVIDTYEYD
IVHCHTPVGGVLARLAARQARRHGTKVLYTAHGFHFCKGAPMKNWLLYYPVEKWLSAYTDCLITINEEDYIRAKGLQRPG
GRTQKIHGIGVNTERFRPVSPIEQQRLREKHGFREDDFILVYPAELNLNKNQKQLIEAAALLKEKIPSLRLVFAGEGAME
HTYQTLAEKLGASAHVCFYGFCSDIHELIQLADVSVASSIREGLGMNVLEGMAAEQPAIATDNRGHREIIRDGENGFLIK
IGDSAAFARRIEQLYHKPELCRKLGQEGRKTALRFSEARTVEEMADIYSAYMDMDTKEKSV
>Mature_380_residues
TKKILFCATVDYHFKAFHLPYFKWFKQMGWEVHVAANGQTKLPYVDEKFSIPIRRSPFDPQNLAVYRQLKKVIDTYEYDI
VHCHTPVGGVLARLAARQARRHGTKVLYTAHGFHFCKGAPMKNWLLYYPVEKWLSAYTDCLITINEEDYIRAKGLQRPGG
RTQKIHGIGVNTERFRPVSPIEQQRLREKHGFREDDFILVYPAELNLNKNQKQLIEAAALLKEKIPSLRLVFAGEGAMEH
TYQTLAEKLGASAHVCFYGFCSDIHELIQLADVSVASSIREGLGMNVLEGMAAEQPAIATDNRGHREIIRDGENGFLIKI
GDSAAFARRIEQLYHKPELCRKLGQEGRKTALRFSEARTVEEMADIYSAYMDMDTKEKSV

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles. Required for biofilm maintenance

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EPSD_BACSU (P71053)

Other databases:

- EMBL:   Z71928
- EMBL:   Z94043
- EMBL:   AL009126
- PIR:   C70036
- RefSeq:   NP_391314.1
- ProteinModelPortal:   P71053
- EnsemblBacteria:   EBBACT00000002326
- GeneID:   938611
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34340
- NMPDR:   fig|224308.1.peg.3440
- GenoList:   BSU34340
- GeneTree:   EBGT00070000032231
- HOGENOM:   HBG338338
- OMA:   CATVDYH
- ProtClustDB:   CLSK887914
- BioCyc:   BSUB:BSU34340-MONOMER
- InterPro:   IPR001296

Pfam domain/function: PF00534 Glycos_transf_1

EC number: NA

Molecular weight: Translated: 43572; Mature: 43440

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKILFCATVDYHFKAFHLPYFKWFKQMGWEVHVAANGQTKLPYVDEKFSIPIRRSPFD
CCCEEEEEEEECCCCEEECCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC
PQNLAVYRQLKKVIDTYEYDIVHCHTPVGGVLARLAARQARRHGTKVLYTAHGFHFCKGA
HHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCEEEECCC
PMKNWLLYYPVEKWLSAYTDCLITINEEDYIRAKGLQRPGGRTQKIHGIGVNTERFRPVS
CCCCCEEEECHHHHHHHHCCEEEEECCCCHHHHCCCCCCCCCCCEEEEECCCCCCCCCCC
PIEQQRLREKHGFREDDFILVYPAELNLNKNQKQLIEAAALLKEKIPSLRLVFAGEGAME
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHH
HTYQTLAEKLGASAHVCFYGFCSDIHELIQLADVSVASSIREGLGMNVLEGMAAEQPAIA
HHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
TDNRGHREIIRDGENGFLIKIGDSAAFARRIEQLYHKPELCRKLGQEGRKTALRFSEART
CCCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
VEEMADIYSAYMDMDTKEKSV
HHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TKKILFCATVDYHFKAFHLPYFKWFKQMGWEVHVAANGQTKLPYVDEKFSIPIRRSPFD
CCEEEEEEEECCCCEEECCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC
PQNLAVYRQLKKVIDTYEYDIVHCHTPVGGVLARLAARQARRHGTKVLYTAHGFHFCKGA
HHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCEEEECCC
PMKNWLLYYPVEKWLSAYTDCLITINEEDYIRAKGLQRPGGRTQKIHGIGVNTERFRPVS
CCCCCEEEECHHHHHHHHCCEEEEECCCCHHHHCCCCCCCCCCCEEEEECCCCCCCCCCC
PIEQQRLREKHGFREDDFILVYPAELNLNKNQKQLIEAAALLKEKIPSLRLVFAGEGAME
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHH
HTYQTLAEKLGASAHVCFYGFCSDIHELIQLADVSVASSIREGLGMNVLEGMAAEQPAIA
HHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
TDNRGHREIIRDGENGFLIKIGDSAAFARRIEQLYHKPELCRKLGQEGRKTALRFSEART
CCCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
VEEMADIYSAYMDMDTKEKSV
HHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Hexosyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377