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The map label for this gene is epsC

Identifier: 16080488

GI number: 16080488

Start: 3526407

End: 3528203

Strand: Reverse

Name: epsC

Synonym: BSU34350

Alternate gene names: 16080488

Gene position: 3528203-3526407 (Counterclockwise)

Preceding gene: 16080489

Following gene: 16080487

Centisome position: 83.69

GC content: 46.91

Gene sequence:

>1797_bases
ATGATTATTGCGCTGGATACTTACCTCGTTTTAAATTCAGTTATTGCAGGATATCAATTTTTAAAAGATTCCTATCAATT
TTATGACTCCGGAGCATTACTGCTTACCGCTGTCAGCTTGCTCCTCAGCTATCATGTGTGTGCTTTCCTGTTCAATCAGT
ATAAACAGGTGTGGACATACACCGGGCTTGGCGAGCTGATTGTCCTGCTTAAGGGCATTACGCTTTCAGCCGCTGTGACC
GGCGTCATTCAGTATGCTGTGTATCACACGATGTTCTTCCGTCTGTTAACCGCGTGCTGGGTGCTTCAGCTTTTGTCTAT
TGGAGGGACCCGTATTTTATCCAGAGTATTAAACGAAAGCATCAGGAAAAAACGCTGCGCCTCGTCCCGCGCGCTGATTA
TCGGGGCGGGCTCAGGTGGGACTCTGATGGTCAGGCAGCTGCTTTCGAAAGATGAACCTGATATCATACCTGTCGCTTTT
ATTGATGACGACCAAACGAAGCATAAATTAGAAATTATGGGGCTGCCCGTAATCGGCGGAAAAGAAAGTATCATGCCTGC
GGTGCAAAAGCTCAAAATTAATTATATTATTATTGCCATTCCTTCACTCCGCACCCATGAGCTTCAGGTGTTATATAAAG
AATGTGTGCGAACCGGAGTAAGCATTAAAATTATGCCTCATTTTGATGAAATGCTGCTTGGCACACGAACTGCCGGACAA
ATCAGAGATGTAAAAGCTGAGGACTTGCTCGGCAGAAAGCCGGTAACCCTCGACACTAGCGAAATTTCGAACCGCATCAA
AGGAAAAACAGTTCTCGTCACGGGAGCGGGCGGATCAATCGGCTCGGAAATCTGCCGTCAGATCAGCGCGTTTCAGCCTA
AGGAAATCATTCTGCTCGGCCATGGGGAAAACAGCATTCATTCGATTTATACAGAGCTGAACGGACGATTCGGCAAACAC
ATTGTGTTCCATACGGAAATCGCTGATGTGCAGGACCGCGATAAAATGTTTACCTTGATGAAAAAATACGAGCCGCATGT
TGTCTATCATGCAGCTGCCCATAAGCATGTGCCTTTAATGGAACACAATCCAGAAGAGGCGGTCAAAAACAATATTATCG
GAACAAAAAATGTCGCGGAAGCAGCCGATATGTCGGGAACTGAGACATTCGTGCTGATTTCATCGGACAAAGCGGTGAAC
CCAGCCAACGTAATGGGGGCGACAAAACGATTCGCAGAGATGATTATTATGAATCTTGGGAAAGTCAGCAGAACCAAATT
TGTTGCTGTTCGCTTCGGCAATGTACTCGGGAGCCGCGGCAGCGTCATTCCAATTTTCAAAAAACAGATTGAAAAAGGCG
GCCCGGTGACAGTAACACATCCGGCAATGACCCGCTATTTCATGACGATTCCCGAGGCATCAAGGCTTGTGATTCAGGCT
GGGGCACTGGCGAAAGGGCGTCAAATTTTCGTTCTCGATATGGGAGAGCCCGTAAAGATTGTGGATCTTGCCAAAAACCT
CATTCATTTGTCCGGCTACACGACTGAGCAGGTTCCAATCGAATTCACAGGCATTCGTCCGGGCGAAAAAATGTATGAAG
AATTGCTGAACAAAAATGAAGTCCATGCTGAACAAATCTTTCCAAAAATTCACATCGGTAAAGCGGTGGACGGCGATTGG
CCGGTGCTGATGCGCTTTATCGAGGATTTTCATGAGCTGCCGGAAGCCGACCTGAGAGCGAGGCTGTTTGCGGCAATCAA
TACATCAGAAGAAATGACGGCTGCCAGCGTTCATTAG

Upstream 100 bases:

>100_bases
GTTTAGCTGCTGCCGATTTTTTGGAGAGACGAAAACCGCCGCAGCTAAACCGATAAAAGAATTAGGGGGGAAAAGGTTGA
GTTACCGGAGAAGACTGTCA

Downstream 100 bases:

>100_bases
GGGGAGTGAAAATGACGAAAAAGATATTGTTTTGCGCGACTGTTGATTATCATTTTAAGGCCTTTCACCTCCCTTATTTT
AAATGGTTCAAGCAAATGGG

Product: UDP-sugar epimerase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MIIALDTYLVLNSVIAGYQFLKDSYQFYDSGALLLTAVSLLLSYHVCAFLFNQYKQVWTYTGLGELIVLLKGITLSAAVT
GVIQYAVYHTMFFRLLTACWVLQLLSIGGTRILSRVLNESIRKKRCASSRALIIGAGSGGTLMVRQLLSKDEPDIIPVAF
IDDDQTKHKLEIMGLPVIGGKESIMPAVQKLKINYIIIAIPSLRTHELQVLYKECVRTGVSIKIMPHFDEMLLGTRTAGQ
IRDVKAEDLLGRKPVTLDTSEISNRIKGKTVLVTGAGGSIGSEICRQISAFQPKEIILLGHGENSIHSIYTELNGRFGKH
IVFHTEIADVQDRDKMFTLMKKYEPHVVYHAAAHKHVPLMEHNPEEAVKNNIIGTKNVAEAADMSGTETFVLISSDKAVN
PANVMGATKRFAEMIIMNLGKVSRTKFVAVRFGNVLGSRGSVIPIFKKQIEKGGPVTVTHPAMTRYFMTIPEASRLVIQA
GALAKGRQIFVLDMGEPVKIVDLAKNLIHLSGYTTEQVPIEFTGIRPGEKMYEELLNKNEVHAEQIFPKIHIGKAVDGDW
PVLMRFIEDFHELPEADLRARLFAAINTSEEMTAASVH

Sequences:

>Translated_598_residues
MIIALDTYLVLNSVIAGYQFLKDSYQFYDSGALLLTAVSLLLSYHVCAFLFNQYKQVWTYTGLGELIVLLKGITLSAAVT
GVIQYAVYHTMFFRLLTACWVLQLLSIGGTRILSRVLNESIRKKRCASSRALIIGAGSGGTLMVRQLLSKDEPDIIPVAF
IDDDQTKHKLEIMGLPVIGGKESIMPAVQKLKINYIIIAIPSLRTHELQVLYKECVRTGVSIKIMPHFDEMLLGTRTAGQ
IRDVKAEDLLGRKPVTLDTSEISNRIKGKTVLVTGAGGSIGSEICRQISAFQPKEIILLGHGENSIHSIYTELNGRFGKH
IVFHTEIADVQDRDKMFTLMKKYEPHVVYHAAAHKHVPLMEHNPEEAVKNNIIGTKNVAEAADMSGTETFVLISSDKAVN
PANVMGATKRFAEMIIMNLGKVSRTKFVAVRFGNVLGSRGSVIPIFKKQIEKGGPVTVTHPAMTRYFMTIPEASRLVIQA
GALAKGRQIFVLDMGEPVKIVDLAKNLIHLSGYTTEQVPIEFTGIRPGEKMYEELLNKNEVHAEQIFPKIHIGKAVDGDW
PVLMRFIEDFHELPEADLRARLFAAINTSEEMTAASVH
>Mature_598_residues
MIIALDTYLVLNSVIAGYQFLKDSYQFYDSGALLLTAVSLLLSYHVCAFLFNQYKQVWTYTGLGELIVLLKGITLSAAVT
GVIQYAVYHTMFFRLLTACWVLQLLSIGGTRILSRVLNESIRKKRCASSRALIIGAGSGGTLMVRQLLSKDEPDIIPVAF
IDDDQTKHKLEIMGLPVIGGKESIMPAVQKLKINYIIIAIPSLRTHELQVLYKECVRTGVSIKIMPHFDEMLLGTRTAGQ
IRDVKAEDLLGRKPVTLDTSEISNRIKGKTVLVTGAGGSIGSEICRQISAFQPKEIILLGHGENSIHSIYTELNGRFGKH
IVFHTEIADVQDRDKMFTLMKKYEPHVVYHAAAHKHVPLMEHNPEEAVKNNIIGTKNVAEAADMSGTETFVLISSDKAVN
PANVMGATKRFAEMIIMNLGKVSRTKFVAVRFGNVLGSRGSVIPIFKKQIEKGGPVTVTHPAMTRYFMTIPEASRLVIQA
GALAKGRQIFVLDMGEPVKIVDLAKNLIHLSGYTTEQVPIEFTGIRPGEKMYEELLNKNEVHAEQIFPKIHIGKAVDGDW
PVLMRFIEDFHELPEADLRARLFAAINTSEEMTAASVH

Specific function: Involved in biofilm formation

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EPSC_BACSU (P71052)

Other databases:

- EMBL:   Z71928
- EMBL:   Z94043
- EMBL:   AL009126
- PIR:   B70036
- RefSeq:   NP_391315.1
- HSSP:   O25511
- ProteinModelPortal:   P71052
- SMR:   P71052
- EnsemblBacteria:   EBBACT00000003857
- GeneID:   938571
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU34350
- NMPDR:   fig|224308.1.peg.3441
- GenoList:   BSU34350
- GeneTree:   EBGT00050000000675
- HOGENOM:   HBG429349
- OMA:   ILSPRAD
- ProtClustDB:   CLSK887915
- BioCyc:   BSUB:BSU34350-MONOMER
- GO:   GO:0005488
- InterPro:   IPR016040
- InterPro:   IPR003869
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10366:SF23

Pfam domain/function: PF02719 Polysacc_synt_2

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 66268; Mature: 66268

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x120d57e8)-; HASH(0x40bf4820)-; HASH(0x1231945c)-; HASH(0x123f2e6c)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIALDTYLVLNSVIAGYQFLKDSYQFYDSGALLLTAVSLLLSYHVCAFLFNQYKQVWTY
CEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGLGELIVLLKGITLSAAVTGVIQYAVYHTMFFRLLTACWVLQLLSIGGTRILSRVLNES
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
IRKKRCASSRALIIGAGSGGTLMVRQLLSKDEPDIIPVAFIDDDQTKHKLEIMGLPVIGG
HHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEEEEECC
KESIMPAVQKLKINYIIIAIPSLRTHELQVLYKECVRTGVSIKIMPHFDEMLLGTRTAGQ
CHHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCCCC
IRDVKAEDLLGRKPVTLDTSEISNRIKGKTVLVTGAGGSIGSEICRQISAFQPKEIILLG
HHCCCHHHHCCCCCCEEEHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEE
HGENSIHSIYTELNGRFGKHIVFHTEIADVQDRDKMFTLMKKYEPHVVYHAAAHKHVPLM
CCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCC
EHNPEEAVKNNIIGTKNVAEAADMSGTETFVLISSDKAVNPANVMGATKRFAEMIIMNLG
CCCHHHHHHCCCCCCCCHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCC
KVSRTKFVAVRFGNVLGSRGSVIPIFKKQIEKGGPVTVTHPAMTRYFMTIPEASRLVIQA
CCCCCEEEEEEECHHHCCCCCEEHHHHHHHCCCCCEEEECHHHHHHEEECCCCCEEEEEE
GALAKGRQIFVLDMGEPVKIVDLAKNLIHLSGYTTEQVPIEFTGIRPGEKMYEELLNKNE
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHCCCCC
VHAEQIFPKIHIGKAVDGDWPVLMRFIEDFHELPEADLRARLFAAINTSEEMTAASVH
CCHHHHCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHCCCH
>Mature Secondary Structure
MIIALDTYLVLNSVIAGYQFLKDSYQFYDSGALLLTAVSLLLSYHVCAFLFNQYKQVWTY
CEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGLGELIVLLKGITLSAAVTGVIQYAVYHTMFFRLLTACWVLQLLSIGGTRILSRVLNES
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
IRKKRCASSRALIIGAGSGGTLMVRQLLSKDEPDIIPVAFIDDDQTKHKLEIMGLPVIGG
HHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEEEEECC
KESIMPAVQKLKINYIIIAIPSLRTHELQVLYKECVRTGVSIKIMPHFDEMLLGTRTAGQ
CHHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCCCC
IRDVKAEDLLGRKPVTLDTSEISNRIKGKTVLVTGAGGSIGSEICRQISAFQPKEIILLG
HHCCCHHHHCCCCCCEEEHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEE
HGENSIHSIYTELNGRFGKHIVFHTEIADVQDRDKMFTLMKKYEPHVVYHAAAHKHVPLM
CCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCC
EHNPEEAVKNNIIGTKNVAEAADMSGTETFVLISSDKAVNPANVMGATKRFAEMIIMNLG
CCCHHHHHHCCCCCCCCHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCC
KVSRTKFVAVRFGNVLGSRGSVIPIFKKQIEKGGPVTVTHPAMTRYFMTIPEASRLVIQA
CCCCCEEEEEEECHHHCCCCCEEHHHHHHHCCCCCEEEECHHHHHHEEECCCCCEEEEEE
GALAKGRQIFVLDMGEPVKIVDLAKNLIHLSGYTTEQVPIEFTGIRPGEKMYEELLNKNE
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHCCCCC
VHAEQIFPKIHIGKAVDGDWPVLMRFIEDFHELPEADLRARLFAAINTSEEMTAASVH
CCHHHHCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377