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The map label for this gene is yvaE

Identifier: 16080410

GI number: 16080410

Start: 3448693

End: 3449052

Strand: Reverse

Name: yvaE

Synonym: BSU33570

Alternate gene names: 16080410

Gene position: 3449052-3448693 (Counterclockwise)

Preceding gene: 16080411

Following gene: 16080409

Centisome position: 81.82

GC content: 40.56

Gene sequence:

>360_bases
ATGAATTGGGTGTTTCTATGCTTGGCGATTTTGTTTGAGGTTGCGGGAACTGTCAGCATGAAGCTATCGTCTGGTTTTAC
AAAGCTTATTCCCAGTTTGCTGTTGATTTTCTTTTATGGAGGGAGTCTTTTCTTTCTGACGCTTACGCTTAAATCCATTG
ATGTATCAGTTGCTTATGCTGTATGGTCAGGGATGGGAATTGTCTTGATTACAGTCGTTGGTTTTTTGTTTTTTCAAGAA
CATGTGAGTGTCATGAAAGTCATTTCAATTGGATTGATTATTGCCGGTGTTGTGTCTTTAAACCTGATCGAGCATGTTGC
GGTGTCTGAGCCGGTACATAAAAGCGGGCAGTACAAATGA

Upstream 100 bases:

>100_bases
AATATCCGCTGAAACATCAGTTCACGACAGGAGAGGTACTTGAATCAATTGTAGATGTTTTGTTTAACGGTATAACAGCT
GATGAGGAGGCTGATGCCAC

Downstream 100 bases:

>100_bases
GATATATGAAGCTGTTTTTTCTCGTGACTGATATCGGTTTTATCTTGTACTGGCTGTCAGCCGGATTTTCGCTCATACCG
GAAAGCTGGGCTTTTAAACA

Product: metabolite-efflux transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 119; Mature: 119

Protein sequence:

>119_residues
MNWVFLCLAILFEVAGTVSMKLSSGFTKLIPSLLLIFFYGGSLFFLTLTLKSIDVSVAYAVWSGMGIVLITVVGFLFFQE
HVSVMKVISIGLIIAGVVSLNLIEHVAVSEPVHKSGQYK

Sequences:

>Translated_119_residues
MNWVFLCLAILFEVAGTVSMKLSSGFTKLIPSLLLIFFYGGSLFFLTLTLKSIDVSVAYAVWSGMGIVLITVVGFLFFQE
HVSVMKVISIGLIIAGVVSLNLIEHVAVSEPVHKSGQYK
>Mature_119_residues
MNWVFLCLAILFEVAGTVSMKLSSGFTKLIPSLLLIFFYGGSLFFLTLTLKSIDVSVAYAVWSGMGIVLITVVGFLFFQE
HVSVMKVISIGLIIAGVVSLNLIEHVAVSEPVHKSGQYK

Specific function: Multidrug Transporter. Confers Resistance To A Wide Range Of Toxic Compounds By Removing Them For The Cells. The Efflux Is Coupled To An Influx Of Protons. Probably Prevents The Incorporation Of Methyl VIologen Into Cells. Involved In Ethidium Bromide Ef

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family

Homologues:

Organism=Escherichia coli, GI1786755, Length=106, Percent_Identity=44.3396226415094, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI87082378, Length=105, Percent_Identity=35.2380952380952, Blast_Score=71, Evalue=2e-14,
Organism=Escherichia coli, GI1787884, Length=99, Percent_Identity=40.4040404040404, Blast_Score=64, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YVAE_BACSU (O32227)

Other databases:

- EMBL:   AL009126
- PIR:   C70027
- RefSeq:   NP_391237.1
- ProteinModelPortal:   O32227
- EnsemblBacteria:   EBBACT00000003490
- GeneID:   936156
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU33570
- NMPDR:   fig|224308.1.peg.3363
- GenoList:   BSU33570
- GeneTree:   EBGT00050000001580
- HOGENOM:   HBG740972
- OMA:   SINVIKI
- PhylomeDB:   O32227
- BioCyc:   BSUB:BSU33570-MONOMER
- GO:   GO:0006810
- InterPro:   IPR000390

Pfam domain/function: PF00893 Multi_Drug_Res

EC number: NA

Molecular weight: Translated: 12959; Mature: 12959

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1293b0e8)-; HASH(0x12757318)-; HASH(0x12729430)-; HASH(0x12708054)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWVFLCLAILFEVAGTVSMKLSSGFTKLIPSLLLIFFYGGSLFFLTLTLKSIDVSVAYA
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VWSGMGIVLITVVGFLFFQEHVSVMKVISIGLIIAGVVSLNLIEHVAVSEPVHKSGQYK
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
>Mature Secondary Structure
MNWVFLCLAILFEVAGTVSMKLSSGFTKLIPSLLLIFFYGGSLFFLTLTLKSIDVSVAYA
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VWSGMGIVLITVVGFLFFQEHVSVMKVISIGLIIAGVVSLNLIEHVAVSEPVHKSGQYK
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377