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The map label for this gene is pucG

Identifier: 16080305

GI number: 16080305

Start: 3341166

End: 3342416

Strand: Reverse

Name: pucG

Synonym: BSU32520

Alternate gene names: 16080305

Gene position: 3342416-3341166 (Counterclockwise)

Preceding gene: 16080306

Following gene: 16080304

Centisome position: 79.29

GC content: 52.2

Gene sequence:

>1251_bases
GTGTCAGGCAGAAGAGAATTATGCACCCCGTTAAGAACGATTATGACACCGGGGCCTGTTGAGGTTGATCCGCGTGTATT
AAGAGTAATGAGCACCCCGGTTGTCGGCCAATTTGATCCGGCGTTTACAGGTATTATGAATGAAACGATGGAGATGCTTC
GGGAGCTGTTCCAAACAAAAAACCGCTGGGCATACCCGATTGACGGCACTTCACGGGCGGGAATTGAAGCAGTGCTGGCT
AGTGTGATAGAGCCGGAAGATGATGTGCTCATTCCCATTTACGGCCGTTTCGGTTATTTGCTGACTGAAATCGCCGAGCG
ATATGGGGCAAATGTTCATATGCTGGAGTGCGAATGGGGAACGGTTTTTGACCCGGAGGACATCATACGGGAGATAAAAA
AAGTGAAGCCGAAAATTGTGGCCATGGTGCATGGAGAAACATCGACAGGACGGATTCACCCGCTGAAAGCCATCGGCGAA
GCCTGCCGCACCGAGGATGCATTGTTTATCGTCGATGCAGTCGCAACGATTGGGGGCTGCGAGGTTAAAGTCGATGAATG
GAAGATTGACGCTGCCATCGGCGGAACGCAAAAATGTTTGTCTGTGCCTTCAGGAATGGCGCCGATTACATATAATGAGC
GGGTGGCGGACGTGATTGCAGCTCGCAAAAAGGTGGAACGCGGGATTGCGACACAAGCTGACAGGGCAGCACTTTCGGGA
AATCGGCCAATTACAAGCAACTACTTTGATTTGAGCCAGCTTGAGGATTATTGGAGCGAAAGACGGCTCAATCATCATAC
AGAAGCGACGACGATGCTGTACGCCTTACGTGAGGGAGTCAGACTGGTGCTTGAAGAAGGTCTTGAGACGCGTTTCGAGC
GGCATCGGCACCATGAAGCCGCATTAGCAGCAGGGATAAAGGCAATGGGGCTAAGATTGTTTGGAGATGACAGCTGCAAA
ATGCCTGTTGTCACCTGTGTCGAAATTCCCGGCGGCATTGACGGCGAGTCTGTCCGTGACATGCTGCTGGCACAATTCGG
CATTGAAATCGCCAGCTCATTCGGCCCGCTTGCAGGGAAGATCTGGAGGATCGGCACGATGGGGTACAGCTGCAGAAAAG
AAAACGTGCTATTCGTGCTTGCCGGGTTAGAGGCTGTGCTTCTCAGACATAACGCAGGAATCGAAGCAGGAAAAGCGCTC
CAAGCAGCGCTGGATGTGTATGAAAACGCTGGCCGCCAGGCAGCGGTGTAA

Upstream 100 bases:

>100_bases
TCACCGAAGGAATATACGTCAGCCAGACAGCTTGAGATCGGCGTCCGCGCACTGACTGATTTATTATACAAACTGGCTTA
CTGATAAAGGAGGAATGGCT

Downstream 100 bases:

>100_bases
AGAAAAGCTTGCGGAACTCCGCAAGCTTTTTAACGATATGATTTCGTGTTTTCTCCAATCTGATTTTCTTCGTTTGACTC
TTTTTACAAATCCTCTTTCT

Product: ureidoglycolate lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSGRRELCTPLRTIMTPGPVEVDPRVLRVMSTPVVGQFDPAFTGIMNETMEMLRELFQTKNRWAYPIDGTSRAGIEAVLA
SVIEPEDDVLIPIYGRFGYLLTEIAERYGANVHMLECEWGTVFDPEDIIREIKKVKPKIVAMVHGETSTGRIHPLKAIGE
ACRTEDALFIVDAVATIGGCEVKVDEWKIDAAIGGTQKCLSVPSGMAPITYNERVADVIAARKKVERGIATQADRAALSG
NRPITSNYFDLSQLEDYWSERRLNHHTEATTMLYALREGVRLVLEEGLETRFERHRHHEAALAAGIKAMGLRLFGDDSCK
MPVVTCVEIPGGIDGESVRDMLLAQFGIEIASSFGPLAGKIWRIGTMGYSCRKENVLFVLAGLEAVLLRHNAGIEAGKAL
QAALDVYENAGRQAAV

Sequences:

>Translated_416_residues
MSGRRELCTPLRTIMTPGPVEVDPRVLRVMSTPVVGQFDPAFTGIMNETMEMLRELFQTKNRWAYPIDGTSRAGIEAVLA
SVIEPEDDVLIPIYGRFGYLLTEIAERYGANVHMLECEWGTVFDPEDIIREIKKVKPKIVAMVHGETSTGRIHPLKAIGE
ACRTEDALFIVDAVATIGGCEVKVDEWKIDAAIGGTQKCLSVPSGMAPITYNERVADVIAARKKVERGIATQADRAALSG
NRPITSNYFDLSQLEDYWSERRLNHHTEATTMLYALREGVRLVLEEGLETRFERHRHHEAALAAGIKAMGLRLFGDDSCK
MPVVTCVEIPGGIDGESVRDMLLAQFGIEIASSFGPLAGKIWRIGTMGYSCRKENVLFVLAGLEAVLLRHNAGIEAGKAL
QAALDVYENAGRQAAV
>Mature_415_residues
SGRRELCTPLRTIMTPGPVEVDPRVLRVMSTPVVGQFDPAFTGIMNETMEMLRELFQTKNRWAYPIDGTSRAGIEAVLAS
VIEPEDDVLIPIYGRFGYLLTEIAERYGANVHMLECEWGTVFDPEDIIREIKKVKPKIVAMVHGETSTGRIHPLKAIGEA
CRTEDALFIVDAVATIGGCEVKVDEWKIDAAIGGTQKCLSVPSGMAPITYNERVADVIAARKKVERGIATQADRAALSGN
RPITSNYFDLSQLEDYWSERRLNHHTEATTMLYALREGVRLVLEEGLETRFERHRHHEAALAAGIKAMGLRLFGDDSCKM
PVVTCVEIPGGIDGESVRDMLLAQFGIEIASSFGPLAGKIWRIGTMGYSCRKENVLFVLAGLEAVLLRHNAGIEAGKALQ
AALDVYENAGRQAAV

Specific function: Could encode ureidoglycolase or L-alanine:glyoxylate aminotransferase, or perhaps both activities

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Homo sapiens, GI4557289, Length=387, Percent_Identity=32.0413436692506, Blast_Score=222, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI17536281, Length=384, Percent_Identity=32.5520833333333, Blast_Score=227, Evalue=8e-60,
Organism=Saccharomyces cerevisiae, GI6321079, Length=386, Percent_Identity=23.5751295336788, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17530823, Length=397, Percent_Identity=34.2569269521411, Blast_Score=238, Evalue=7e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUCG_BACSU (O32148)

Other databases:

- EMBL:   AL009126
- PIR:   F70017
- RefSeq:   NP_391132.1
- PDB:   3ISL
- PDBsum:   3ISL
- ProteinModelPortal:   O32148
- SMR:   O32148
- EnsemblBacteria:   EBBACT00000003505
- GeneID:   936676
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32520
- NMPDR:   fig|224308.1.peg.3258
- GenoList:   BSU32520
- GeneTree:   EBGT00050000000896
- HOGENOM:   HBG700056
- OMA:   GHLLCEI
- PhylomeDB:   O32148
- ProtClustDB:   CLSK872815
- BioCyc:   BSUB:BSU32520-MONOMER
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: 2.6.1.-

Molecular weight: Translated: 45743; Mature: 45612

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRRELCTPLRTIMTPGPVEVDPRVLRVMSTPVVGQFDPAFTGIMNETMEMLRELFQTK
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
NRWAYPIDGTSRAGIEAVLASVIEPEDDVLIPIYGRFGYLLTEIAERYGANVHMLECEWG
CCEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEECCC
TVFDPEDIIREIKKVKPKIVAMVHGETSTGRIHPLKAIGEACRTEDALFIVDAVATIGGC
CCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHCCCC
EVKVDEWKIDAAIGGTQKCLSVPSGMAPITYNERVADVIAARKKVERGIATQADRAALSG
EEEEEEEEEEECCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
NRPITSNYFDLSQLEDYWSERRLNHHTEATTMLYALREGVRLVLEEGLETRFERHRHHEA
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAAGIKAMGLRLFGDDSCKMPVVTCVEIPGGIDGESVRDMLLAQFGIEIASSFGPLAGK
HHHHHHHHHCEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
IWRIGTMGYSCRKENVLFVLAGLEAVLLRHNAGIEAGKALQAALDVYENAGRQAAV
HEEEECCCCCCCCCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SGRRELCTPLRTIMTPGPVEVDPRVLRVMSTPVVGQFDPAFTGIMNETMEMLRELFQTK
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
NRWAYPIDGTSRAGIEAVLASVIEPEDDVLIPIYGRFGYLLTEIAERYGANVHMLECEWG
CCEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEECCC
TVFDPEDIIREIKKVKPKIVAMVHGETSTGRIHPLKAIGEACRTEDALFIVDAVATIGGC
CCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHCCCC
EVKVDEWKIDAAIGGTQKCLSVPSGMAPITYNERVADVIAARKKVERGIATQADRAALSG
EEEEEEEEEEECCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
NRPITSNYFDLSQLEDYWSERRLNHHTEATTMLYALREGVRLVLEEGLETRFERHRHHEA
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAAGIKAMGLRLFGDDSCKMPVVTCVEIPGGIDGESVRDMLLAQFGIEIASSFGPLAGK
HHHHHHHHHCEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
IWRIGTMGYSCRKENVLFVLAGLEAVLLRHNAGIEAGKALQAALDVYENAGRQAAV
HEEEECCCCCCCCCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136