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The map label for this gene is pucA

Identifier: 16080304

GI number: 16080304

Start: 3339945

End: 3340937

Strand: Reverse

Name: pucA

Synonym: BSU32510

Alternate gene names: 16080304

Gene position: 3340937-3339945 (Counterclockwise)

Preceding gene: 16080305

Following gene: 255767746

Centisome position: 79.25

GC content: 48.54

Gene sequence:

>993_bases
TTGGGGAATTTTCACACGATGTTAGATGCGCTCCTGGAAGACCAGGAGGAGGCTGTACTGGCGACAATTGTTCAAGTTGA
AGGCTCCGCGTATCGAAAAGCAGGCGCCTCTATGCTCTTTAAGAAAAAAGGCAGACGGATTGGCTTGCTGAGCGGCGGGT
GTGTGGAAGAAGATGTATTCCAAAGAATCAGTGCGCTGGGCGATCAGCTCACATCAACGCTGATTCCCTACGACATGCGG
TCGGAGGACGATCTTTCTTGGGGCATGGGAGCCGGGTGCAATGGCATTATTCATGTTCATGCAGAGCGAATCACTCAGGA
AAAAAGGCGGCACTATGAGAAAGTGAGGGACTGCCTTCATTCAGGCAAAGCTGTCACTTCCGTTATAAAGATTGAATCCT
CCCATTATTTATTTCTGACGGAGAACGGACATTTTGGGAACTGGCCGGACGCGCCTCTGCAAGATATTCAACGTACTGTT
TCAACGCTTCATTTACCGCATTTCGATCAAACCACTAACATGTTTATTCAGCGAATTGAACCGAAGCCGCGTCTCATTCT
GTTTGGGGCGGGACCGGATAATGTACCGCTGGCCAATTTGGCGGCTGACACAGGGTTTTCTGTTATCGTGACGGATTGGC
GGCCCGCTTATTGTACATCCTCTCTTTTTCCAAAAGCGGATCAGCTGATCACCGCTTTTCCGGAACAAATGCTTAGCGAA
TTTCAATTTTTTCCGCATGATGCAGCTGTTGTGGCAACCCATCATTATCAGCACGATCAAACGATCATCAACTTCTTATT
TTCTCAAAACCTCCATTATATTGGACTGTTGGGTTCGGCAAACCGCACGAAACGGCTGCTGAGCGGAAAACATCCTCCGT
CTCACTTTTACAGTCCGGTCGGGCTGAAAATCGGTGCGGAAGGTCCCGAGGAAATTGCGGTAAGTGTAGTTGCAGAAATC
ATTCAAACAAGAAAACGGGTGGCGGTTGTATGA

Upstream 100 bases:

>100_bases
GTCATTTTTTCTTCCATCGTCTATTCTTTATACAAGTTACCTATAAAATCACGCTGAACTAGTTGTTTCTCACGGCAATT
TTCGAGAGGAGGATGCGGCA

Downstream 100 bases:

>100_bases
GAAGCCCCTATCTGATCGGCGTGTTTCTCGCTGCCGGCAAAAGCAGAAGAATGGGACAAAACAAGCTGGCTTTGCCGCTA
AAAGGAGAAAACATAGGCTC

Product: xanthine dehydrogenase molybdopterin recruitment factor

Products: NA

Alternate protein names: XDHase subunit A

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MGNFHTMLDALLEDQEEAVLATIVQVEGSAYRKAGASMLFKKKGRRIGLLSGGCVEEDVFQRISALGDQLTSTLIPYDMR
SEDDLSWGMGAGCNGIIHVHAERITQEKRRHYEKVRDCLHSGKAVTSVIKIESSHYLFLTENGHFGNWPDAPLQDIQRTV
STLHLPHFDQTTNMFIQRIEPKPRLILFGAGPDNVPLANLAADTGFSVIVTDWRPAYCTSSLFPKADQLITAFPEQMLSE
FQFFPHDAAVVATHHYQHDQTIINFLFSQNLHYIGLLGSANRTKRLLSGKHPPSHFYSPVGLKIGAEGPEEIAVSVVAEI
IQTRKRVAVV

Sequences:

>Translated_330_residues
MGNFHTMLDALLEDQEEAVLATIVQVEGSAYRKAGASMLFKKKGRRIGLLSGGCVEEDVFQRISALGDQLTSTLIPYDMR
SEDDLSWGMGAGCNGIIHVHAERITQEKRRHYEKVRDCLHSGKAVTSVIKIESSHYLFLTENGHFGNWPDAPLQDIQRTV
STLHLPHFDQTTNMFIQRIEPKPRLILFGAGPDNVPLANLAADTGFSVIVTDWRPAYCTSSLFPKADQLITAFPEQMLSE
FQFFPHDAAVVATHHYQHDQTIINFLFSQNLHYIGLLGSANRTKRLLSGKHPPSHFYSPVGLKIGAEGPEEIAVSVVAEI
IQTRKRVAVV
>Mature_329_residues
GNFHTMLDALLEDQEEAVLATIVQVEGSAYRKAGASMLFKKKGRRIGLLSGGCVEEDVFQRISALGDQLTSTLIPYDMRS
EDDLSWGMGAGCNGIIHVHAERITQEKRRHYEKVRDCLHSGKAVTSVIKIESSHYLFLTENGHFGNWPDAPLQDIQRTVS
TLHLPHFDQTTNMFIQRIEPKPRLILFGAGPDNVPLANLAADTGFSVIVTDWRPAYCTSSLFPKADQLITAFPEQMLSEF
QFFPHDAAVVATHHYQHDQTIINFLFSQNLHYIGLLGSANRTKRLLSGKHPPSHFYSPVGLKIGAEGPEEIAVSVVAEII
QTRKRVAVV

Specific function: Oxidizes hypoxanthine and xanthine to uric acid. PucA subunit could exert a molybdenum cofactor recruiting function

COG id: COG1975

COG function: function code O; Xanthine and CO dehydrogenases maturation factor, XdhC/CoxF family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789240, Length=161, Percent_Identity=31.055900621118, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): XDHA_BACSU (O32147)

Other databases:

- EMBL:   AL009126
- PIR:   E70017
- RefSeq:   NP_391131.1
- ProteinModelPortal:   O32147
- SMR:   O32147
- EnsemblBacteria:   EBBACT00000001504
- GeneID:   938865
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32510
- NMPDR:   fig|224308.1.peg.3257
- GenoList:   BSU32510
- GeneTree:   EBGT00050000001929
- HOGENOM:   HBG677650
- OMA:   ASMVVAP
- ProtClustDB:   CLSK873190
- BioCyc:   BSUB:BSU32510-MONOMER
- BRENDA:   1.17.1.4
- InterPro:   IPR003777

Pfam domain/function: PF02625 XdhC_CoxI

EC number: =1.17.1.4

Molecular weight: Translated: 36746; Mature: 36615

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNFHTMLDALLEDQEEAVLATIVQVEGSAYRKAGASMLFKKKGRRIGLLSGGCVEEDVF
CCHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHH
QRISALGDQLTSTLIPYDMRSEDDLSWGMGAGCNGIIHVHAERITQEKRRHYEKVRDCLH
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
SGKAVTSVIKIESSHYLFLTENGHFGNWPDAPLQDIQRTVSTLHLPHFDQTTNMFIQRIE
CCHHHHHHEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
PKPRLILFGAGPDNVPLANLAADTGFSVIVTDWRPAYCTSSLFPKADQLITAFPEQMLSE
CCCCEEEEECCCCCCCHHHHHHCCCCEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHH
FQFFPHDAAVVATHHYQHDQTIINFLFSQNLHYIGLLGSANRTKRLLSGKHPPSHFYSPV
HHCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCCCHHCCCCC
GLKIGAEGPEEIAVSVVAEIIQTRKRVAVV
CEEECCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GNFHTMLDALLEDQEEAVLATIVQVEGSAYRKAGASMLFKKKGRRIGLLSGGCVEEDVF
CHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHH
QRISALGDQLTSTLIPYDMRSEDDLSWGMGAGCNGIIHVHAERITQEKRRHYEKVRDCLH
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
SGKAVTSVIKIESSHYLFLTENGHFGNWPDAPLQDIQRTVSTLHLPHFDQTTNMFIQRIE
CCHHHHHHEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
PKPRLILFGAGPDNVPLANLAADTGFSVIVTDWRPAYCTSSLFPKADQLITAFPEQMLSE
CCCCEEEEECCCCCCCHHHHHHCCCCEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHH
FQFFPHDAAVVATHHYQHDQTIINFLFSQNLHYIGLLGSANRTKRLLSGKHPPSHFYSPV
HHCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCCCHHCCCCC
GLKIGAEGPEEIAVSVVAEIIQTRKRVAVV
CEEECCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136