Click here to switch to the map view.

The map label for this gene is pucB

Identifier: 255767746

GI number: 255767746

Start: 3339331

End: 3339948

Strand: Reverse

Name: pucB

Synonym: BSU32500

Alternate gene names: 255767746

Gene position: 3339948-3339331 (Counterclockwise)

Preceding gene: 16080304

Following gene: 16080302

Centisome position: 79.23

GC content: 52.91

Gene sequence:

>618_bases
ATGAGAAGCCCCTATCTGATCGGCGTGTTTCTCGCTGCCGGCAAAAGCAGAAGAATGGGACAAAACAAGCTGGCTTTGCC
GCTAAAAGGAGAAAACATAGGCTCACTTTCCTTGAAAACTGCTCTGTCGTCCCGCCTTGATCACGTGCTGGTCGTCGAGC
GGACAGAACATGCCTCCCTTGAGTGGATAGGAGCGCCGTATCACGCTCCGCCTTTTCAAAAACGCTGGAGCCTGCACGTT
TGCCAAGACGCAGAAAAGGGTCAGGGGCATTCAGTCAGCAGCGGGGTGAGAAAAGCAGAAAGCATGGGGGCGGACGGCAT
TGTCATTTTGTTAGCCGACCAGCCTCAGCTTTCCGTCGATCATCTCAATGCCCTTGTGGCTCTGGCTCCTGAGTCATTTG
CCGTGTCATCGTTTTTAGGGGCGTTCACCCCGCCAATCTACTTCTCGTCGACATGTTTTCCTTATGTAAAGGGATTAAAG
GGAGATGAAGGGGCTCGCAGGCTCCTGAAAAGCGGACAGCTTGGAGCAGGAGCGGTTTTGGAGGCAAAGGATAGCGGTGA
GTTGGACGATATTGATACACCCGAGGAATATGACATGGTAAGGAGGGCGATGTCTTGA

Upstream 100 bases:

>100_bases
ACAGTCCGGTCGGGCTGAAAATCGGTGCGGAAGGTCCCGAGGAAATTGCGGTAAGTGTAGTTGCAGAAATCATTCAAACA
AGAAAACGGGTGGCGGTTGT

Downstream 100 bases:

>100_bases
ACGGCCAAGTGACAAAAGCAAGGATGAATATTCAATTATGGAGACCGGCAGCGCTTGATGAGGCCTATTCGCTTTTAGAG
AAGCTTGCTCCGGATGTGTG

Product: enzyme for molybdopterin cofactor synthesis required for xanthine dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MRSPYLIGVFLAAGKSRRMGQNKLALPLKGENIGSLSLKTALSSRLDHVLVVERTEHASLEWIGAPYHAPPFQKRWSLHV
CQDAEKGQGHSVSSGVRKAESMGADGIVILLADQPQLSVDHLNALVALAPESFAVSSFLGAFTPPIYFSSTCFPYVKGLK
GDEGARRLLKSGQLGAGAVLEAKDSGELDDIDTPEEYDMVRRAMS

Sequences:

>Translated_205_residues
MRSPYLIGVFLAAGKSRRMGQNKLALPLKGENIGSLSLKTALSSRLDHVLVVERTEHASLEWIGAPYHAPPFQKRWSLHV
CQDAEKGQGHSVSSGVRKAESMGADGIVILLADQPQLSVDHLNALVALAPESFAVSSFLGAFTPPIYFSSTCFPYVKGLK
GDEGARRLLKSGQLGAGAVLEAKDSGELDDIDTPEEYDMVRRAMS
>Mature_205_residues
MRSPYLIGVFLAAGKSRRMGQNKLALPLKGENIGSLSLKTALSSRLDHVLVVERTEHASLEWIGAPYHAPPFQKRWSLHV
CQDAEKGQGHSVSSGVRKAESMGADGIVILLADQPQLSVDHLNALVALAPESFAVSSFLGAFTPPIYFSSTCFPYVKGLK
GDEGARRLLKSGQLGAGAVLEAKDSGELDDIDTPEEYDMVRRAMS

Specific function: Required for xanthine dehydrogenase activity. Could be involved in formation of the molybdenum cofactor required by xanthine dehydrogenase

COG id: COG2068

COG function: function code R; Uncharacterized MobA-related protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUCB_BACSU (O32146)

Other databases:

- EMBL:   AL009126
- PIR:   D70017
- RefSeq:   NP_391130.2
- ProteinModelPortal:   O32146
- SMR:   O32146
- EnsemblBacteria:   EBBACT00000004019
- GeneID:   936486
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32500
- NMPDR:   fig|224308.1.peg.3256
- GenoList:   BSU32500
- GeneTree:   EBGT00050000001252
- HOGENOM:   HBG680805
- ProtClustDB:   CLSK2816786
- BioCyc:   BSUB:BSU32500-MONOMER
- InterPro:   IPR017615
- TIGRFAMs:   TIGR03202

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 22092; Mature: 22092

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSPYLIGVFLAAGKSRRMGQNKLALPLKGENIGSLSLKTALSSRLDHVLVVERTEHASL
CCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCE
EWIGAPYHAPPFQKRWSLHVCQDAEKGQGHSVSSGVRKAESMGADGIVILLADQPQLSVD
EEECCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHH
HLNALVALAPESFAVSSFLGAFTPPIYFSSTCFPYVKGLKGDEGARRLLKSGQLGAGAVL
HHHHHEEECCCHHHHHHHHHHCCCCCEECCCHHHHHHCCCCCHHHHHHHHCCCCCCCEEE
EAKDSGELDDIDTPEEYDMVRRAMS
ECCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MRSPYLIGVFLAAGKSRRMGQNKLALPLKGENIGSLSLKTALSSRLDHVLVVERTEHASL
CCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCE
EWIGAPYHAPPFQKRWSLHVCQDAEKGQGHSVSSGVRKAESMGADGIVILLADQPQLSVD
EEECCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHH
HLNALVALAPESFAVSSFLGAFTPPIYFSSTCFPYVKGLKGDEGARRLLKSGQLGAGAVL
HHHHHEEECCCHHHHHHHHHHCCCCCEECCCHHHHHHCCCCCHHHHHHHHCCCCCCCEEE
EAKDSGELDDIDTPEEYDMVRRAMS
ECCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136