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The map label for this gene is bioF

Identifier: 16080074

GI number: 16080074

Start: 3092184

End: 3093353

Strand: Reverse

Name: bioF

Synonym: BSU30220

Alternate gene names: 16080074

Gene position: 3093353-3092184 (Counterclockwise)

Preceding gene: 16080075

Following gene: 16080073

Centisome position: 73.38

GC content: 46.92

Gene sequence:

>1170_bases
TTGAAGATTGATTCCTGGTTAAACGAGCGGTTAGACAGAATGAAAGAAGCCGGCGTACATCGTAACCTGCGGTCAATGGA
TGGAGCGCCGGTTCCAGAGAGGAATATTGATGGCGAAAATCAAACGGTCTGGTCCTCAAACAATTATTTAGGGCTCGCAA
GCGATAGACGTTTGATCGATGCAGCCCAAACAGCATTGCAGCAATTTGGGACAGGAAGCAGCGGTTCACGTTTAACGACA
GGCAATTCGGTCTGGCATGAAAAGCTAGAAAAGAAGATTGCCAGCTTTAAACTGACAGAAGCGGCCCTGCTGTTTTCGAG
CGGTTACTTGGCCAATGTCGGTGTCCTTTCATCCTTGCCAGAAAAGGAAGATGTCATTTTAAGTGACCAGCTCAATCATG
CAAGTATGATCGACGGCTGCCGACTTTCTAAGGCTGATACAGTTGTTTATCGGCATATTGATATGAATGATCTTGAAAAC
AAGCTGAATGAAACACAGCGTTATCAGCGCCGTTTTATCGTAACAGACGGAGTATTCAGCATGGATGGCACAATCGCCCC
TCTTGATCAGATCATCTCACTTGCGAAACGCTATCATGCCTTCGTGGTCGTTGATGATGCCCACGCAACAGGAGTTTTGG
GCGATTCGGGACAAGGAACGAGTGAATACTTTGGTGTTTGTCCCGACATTGTTATCGGCACCTTAAGCAAAGCTGTTGGC
GCGGAAGGAGGTTTTGCGGCAGGATCAGCGGTCTTCATCGACTTTTTGCTGAACCATGCCAGAACATTTATCTTTCAAAC
CGCTATTCCGCCAGCCAGCTGTGCGGCTGCTCACGAGGCTTTCAACATCATTGAAGCCAGCAGGGAAAAACGACAGCTTT
TATTTTCTTATATCAGCATGATCAGAACCAGTCTGAAGAATATGGGTTATGTGGTGAAAGGAGATCACACACCGATTATT
CCTGTAGTCATTGGCGATGCCCATAAAACGGTCCTATTTGCTGAAAAACTGCAGGGCAAGGGAATTTATGCTCCTGCCAT
TCGGCCGCCAACCGTTGCGCCGGGTGAAAGCCGGATTCGAATTACAATCACGTCTGACCACAGTATGGGTGATATTGATC
ATTTGCTGCAAACATTTCATTCAATCGGAAAGGAGCTGCACATCATTTGA

Upstream 100 bases:

>100_bases
GGGGACGTGATTGCATTTCTTCCTCCTCTTGCCAGCACAGCTGAAGAGCTCTCGGAAATGGTTGCCATTATGAAACAAGC
GATCCACGAGGTTACGAGCC

Downstream 100 bases:

>100_bases
GGGGTTTTTTTGTGACGGGAACTGATACAGAAGTAGGGAAAACGGTTATATCCAGCGGTCTTGCTGCCTTATTGAAAGAC
AATAATAGACATGTCGGGGT

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MKIDSWLNERLDRMKEAGVHRNLRSMDGAPVPERNIDGENQTVWSSNNYLGLASDRRLIDAAQTALQQFGTGSSGSRLTT
GNSVWHEKLEKKIASFKLTEAALLFSSGYLANVGVLSSLPEKEDVILSDQLNHASMIDGCRLSKADTVVYRHIDMNDLEN
KLNETQRYQRRFIVTDGVFSMDGTIAPLDQIISLAKRYHAFVVVDDAHATGVLGDSGQGTSEYFGVCPDIVIGTLSKAVG
AEGGFAAGSAVFIDFLLNHARTFIFQTAIPPASCAAAHEAFNIIEASREKRQLLFSYISMIRTSLKNMGYVVKGDHTPII
PVVIGDAHKTVLFAEKLQGKGIYAPAIRPPTVAPGESRIRITITSDHSMGDIDHLLQTFHSIGKELHII

Sequences:

>Translated_389_residues
MKIDSWLNERLDRMKEAGVHRNLRSMDGAPVPERNIDGENQTVWSSNNYLGLASDRRLIDAAQTALQQFGTGSSGSRLTT
GNSVWHEKLEKKIASFKLTEAALLFSSGYLANVGVLSSLPEKEDVILSDQLNHASMIDGCRLSKADTVVYRHIDMNDLEN
KLNETQRYQRRFIVTDGVFSMDGTIAPLDQIISLAKRYHAFVVVDDAHATGVLGDSGQGTSEYFGVCPDIVIGTLSKAVG
AEGGFAAGSAVFIDFLLNHARTFIFQTAIPPASCAAAHEAFNIIEASREKRQLLFSYISMIRTSLKNMGYVVKGDHTPII
PVVIGDAHKTVLFAEKLQGKGIYAPAIRPPTVAPGESRIRITITSDHSMGDIDHLLQTFHSIGKELHII
>Mature_389_residues
MKIDSWLNERLDRMKEAGVHRNLRSMDGAPVPERNIDGENQTVWSSNNYLGLASDRRLIDAAQTALQQFGTGSSGSRLTT
GNSVWHEKLEKKIASFKLTEAALLFSSGYLANVGVLSSLPEKEDVILSDQLNHASMIDGCRLSKADTVVYRHIDMNDLEN
KLNETQRYQRRFIVTDGVFSMDGTIAPLDQIISLAKRYHAFVVVDDAHATGVLGDSGQGTSEYFGVCPDIVIGTLSKAVG
AEGGFAAGSAVFIDFLLNHARTFIFQTAIPPASCAAAHEAFNIIEASREKRQLLFSYISMIRTSLKNMGYVVKGDHTPII
PVVIGDAHKTVLFAEKLQGKGIYAPAIRPPTVAPGESRIRITITSDHSMGDIDHLLQTFHSIGKELHII

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Homo sapiens, GI7657118, Length=386, Percent_Identity=39.6373056994819, Blast_Score=283, Evalue=3e-76,
Organism=Homo sapiens, GI284448556, Length=344, Percent_Identity=41.5697674418605, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI4502025, Length=406, Percent_Identity=32.7586206896552, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI40316939, Length=406, Percent_Identity=32.7586206896552, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI83977442, Length=405, Percent_Identity=32.3456790123457, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI83977440, Length=405, Percent_Identity=32.3456790123457, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI83977444, Length=405, Percent_Identity=32.3456790123457, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI4758668, Length=363, Percent_Identity=31.129476584022, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI119220554, Length=363, Percent_Identity=29.4765840220386, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI5454084, Length=277, Percent_Identity=29.9638989169675, Blast_Score=142, Evalue=7e-34,
Organism=Escherichia coli, GI1790046, Length=389, Percent_Identity=37.7892030848329, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1786993, Length=341, Percent_Identity=36.950146627566, Blast_Score=224, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI71994529, Length=388, Percent_Identity=38.4020618556701, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI32566772, Length=360, Percent_Identity=32.2222222222222, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17560912, Length=360, Percent_Identity=32.2222222222222, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17560914, Length=360, Percent_Identity=32.2222222222222, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI71982617, Length=289, Percent_Identity=29.757785467128, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71982625, Length=289, Percent_Identity=29.757785467128, Blast_Score=135, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6320438, Length=414, Percent_Identity=32.1256038647343, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6320267, Length=364, Percent_Identity=31.043956043956, Blast_Score=182, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6323954, Length=263, Percent_Identity=28.1368821292776, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24662918, Length=393, Percent_Identity=37.4045801526718, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI17137420, Length=344, Percent_Identity=37.7906976744186, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17136286, Length=362, Percent_Identity=29.8342541436464, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24653280, Length=268, Percent_Identity=28.3582089552239, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24653276, Length=268, Percent_Identity=28.3582089552239, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24653278, Length=268, Percent_Identity=28.3582089552239, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_BACSU (P53556)

Other databases:

- EMBL:   U51868
- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   F69594
- RefSeq:   NP_390900.1
- ProteinModelPortal:   P53556
- SMR:   P53556
- EnsemblBacteria:   EBBACT00000003999
- GeneID:   937268
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU30220
- NMPDR:   fig|224308.1.peg.3025
- GenoList:   BSU30220
- GeneTree:   EBGT00050000000589
- HOGENOM:   HBG649839
- OMA:   QGIRPPT
- PhylomeDB:   P53556
- ProtClustDB:   CLSK887748
- BioCyc:   BSUB:BSU30220-MONOMER
- BRENDA:   2.3.1.47
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 42582; Mature: 42582

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 21-21 BINDING 133-133 BINDING 180-180 BINDING 351-351

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDSWLNERLDRMKEAGVHRNLRSMDGAPVPERNIDGENQTVWSSNNYLGLASDRRLID
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEEEECCCCHHHH
AAQTALQQFGTGSSGSRLTTGNSVWHEKLEKKIASFKLTEAALLFSSGYLANVGVLSSLP
HHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
EKEDVILSDQLNHASMIDGCRLSKADTVVYRHIDMNDLENKLNETQRYQRRFIVTDGVFS
CCCCEEECCCCCHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHEEEEECCEEE
MDGTIAPLDQIISLAKRYHAFVVVDDAHATGVLGDSGQGTSEYFGVCPDIVIGTLSKAVG
CCCCCCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHC
AEGGFAAGSAVFIDFLLNHARTFIFQTAIPPASCAAAHEAFNIIEASREKRQLLFSYISM
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRTSLKNMGYVVKGDHTPIIPVVIGDAHKTVLFAEKLQGKGIYAPAIRPPTVAPGESRIR
HHHHHHHCCEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCCEEE
ITITSDHSMGDIDHLLQTFHSIGKELHII
EEEECCCCCCHHHHHHHHHHHHCHHEECC
>Mature Secondary Structure
MKIDSWLNERLDRMKEAGVHRNLRSMDGAPVPERNIDGENQTVWSSNNYLGLASDRRLID
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEEEECCCCHHHH
AAQTALQQFGTGSSGSRLTTGNSVWHEKLEKKIASFKLTEAALLFSSGYLANVGVLSSLP
HHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
EKEDVILSDQLNHASMIDGCRLSKADTVVYRHIDMNDLENKLNETQRYQRRFIVTDGVFS
CCCCEEECCCCCHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHEEEEECCEEE
MDGTIAPLDQIISLAKRYHAFVVVDDAHATGVLGDSGQGTSEYFGVCPDIVIGTLSKAVG
CCCCCCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHC
AEGGFAAGSAVFIDFLLNHARTFIFQTAIPPASCAAAHEAFNIIEASREKRQLLFSYISM
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRTSLKNMGYVVKGDHTPIIPVVIGDAHKTVLFAEKLQGKGIYAPAIRPPTVAPGESRIR
HHHHHHHCCEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCCEEE
ITITSDHSMGDIDHLLQTFHSIGKELHII
EEEECCCCCCHHHHHHHHHHHHCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763940; 9387221; 9384377