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The map label for this gene is bioA

Identifier: 16080075

GI number: 16080075

Start: 3093343

End: 3094689

Strand: Reverse

Name: bioA

Synonym: BSU30230

Alternate gene names: 16080075

Gene position: 3094689-3093343 (Counterclockwise)

Preceding gene: 255767694

Following gene: 16080074

Centisome position: 73.41

GC content: 45.81

Gene sequence:

>1347_bases
ATGACTCATGATTTGATAGAAAAAAGTAAAAAGCACCTCTGGCTGCCATTTACCCAAATGAAAGATTATGATGAAAACCC
CTTAATCATCGAAAGCGGGACTGGAATCAAAGTCAAAGACATAAACGGCAAGGAATACTATGACGGTTTTTCATCGGTTT
GGCTTAATGTCCACGGACACCGCAAAAAAGAACTAGATGACGCCATAAAAAAACAGCTCGGAAAAATTGCGCACTCCACG
TTATTGGGCATGACCAATGTTCCAGCAACCCAGCTTGCCGAAACATTAATCGACATCAGCCCAAAAAAGCTCACGCGGGT
CTTTTATTCAGACAGCGGCGCAGAGGCGATGGAAATAGCCCTAAAAATGGCGTTTCAGTATTGGAAGAACATCGGGAAGC
CCGAGAAACAAAAATTCATCGCAATGAAAAACGGGTATCACGGTGATACGATTGGCGCCGTCAGTGTCGGTTCAATTGAG
CTTTTTCACCACGTATACGGCCCGTTGATGTTCGAGAGTTACAAGGCCCCGATTCCTTATGTGTATCGTTCTGAAAGCGG
TGATCCTGATGAGTGCCGTGATCAGTGCCTCCGAGAGCTTGCACAGCTGCTTGAGGAACATCATGAGGAAATTGCCGCGC
TTTCCATTGAATCAATGGTACAAGGCGCGTCCGGTATGATCGTGATGCCGGAAGGATATTTGGCAGGCGTGCGCGAGCTA
TGTACAACATACGATGTCTTAATGATCGTTGATGAAGTCGCTACAGGCTTTGGCCGTACAGGAAAAATGTTTGCGTGCGA
GCACGAGAATGTCCAGCCTGATCTGATGGCTGCCGGTAAAGGCATTACAGGAGGCTATTTGCCAATTGCCGTTACGTTTG
CCACTGAAGACATCTATAAGGCATTCTATGATGATTATGAAAACCTAAAAACCTTTTTCCATGGCCATTCCTATACAGGC
AATCAGCTTGGCTGTGCGGTTGCGCTTGAAAATCTGGCATTATTTGAATCTGAAAACATTGTGGAACAAGTAGCGGAAAA
AAGTAAAAAGCTCCATTTTCTTCTTCAAGATCTGCACGCTCTTCCTCATGTTGGGGATATTCGGCAGCTTGGCTTTATGT
GCGGTGCAGAGCTTGTACGATCAAAGGAAACTAAAGAACCTTACCCGGCTGATCGGCGGATTGGATACAAAGTTTCCTTA
AAAATGAGAGAGTTAGGAATGCTGACAAGACCGCTTGGGGACGTGATTGCATTTCTTCCTCCTCTTGCCAGCACAGCTGA
AGAGCTCTCGGAAATGGTTGCCATTATGAAACAAGCGATCCACGAGGTTACGAGCCTTGAAGATTGA

Upstream 100 bases:

>100_bases
GAGGGCTGCCGAGTCTTTTTTATTGATGGATCCAATGATGTAAACACGTACATACATGACCTGGAGAAGCAGCCTATTTT
AATAGAGTGGGAGGAAGATC

Downstream 100 bases:

>100_bases
TTCCTGGTTAAACGAGCGGTTAGACAGAATGAAAGAAGCCGGCGTACATCGTAACCTGCGGTCAATGGATGGAGCGCCGG
TTCCAGAGAGGAATATTGAT

Product: adenosylmethionine--8-amino-7-oxononanoate transaminase

Products: S-adenosyl-4-methylthio-2-oxobutanoate; 7,8-diaminononanoate

Alternate protein names: 7,8-diamino-pelargonic acid aminotransferase; DAPA aminotransferase

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MTHDLIEKSKKHLWLPFTQMKDYDENPLIIESGTGIKVKDINGKEYYDGFSSVWLNVHGHRKKELDDAIKKQLGKIAHST
LLGMTNVPATQLAETLIDISPKKLTRVFYSDSGAEAMEIALKMAFQYWKNIGKPEKQKFIAMKNGYHGDTIGAVSVGSIE
LFHHVYGPLMFESYKAPIPYVYRSESGDPDECRDQCLRELAQLLEEHHEEIAALSIESMVQGASGMIVMPEGYLAGVREL
CTTYDVLMIVDEVATGFGRTGKMFACEHENVQPDLMAAGKGITGGYLPIAVTFATEDIYKAFYDDYENLKTFFHGHSYTG
NQLGCAVALENLALFESENIVEQVAEKSKKLHFLLQDLHALPHVGDIRQLGFMCGAELVRSKETKEPYPADRRIGYKVSL
KMRELGMLTRPLGDVIAFLPPLASTAEELSEMVAIMKQAIHEVTSLED

Sequences:

>Translated_448_residues
MTHDLIEKSKKHLWLPFTQMKDYDENPLIIESGTGIKVKDINGKEYYDGFSSVWLNVHGHRKKELDDAIKKQLGKIAHST
LLGMTNVPATQLAETLIDISPKKLTRVFYSDSGAEAMEIALKMAFQYWKNIGKPEKQKFIAMKNGYHGDTIGAVSVGSIE
LFHHVYGPLMFESYKAPIPYVYRSESGDPDECRDQCLRELAQLLEEHHEEIAALSIESMVQGASGMIVMPEGYLAGVREL
CTTYDVLMIVDEVATGFGRTGKMFACEHENVQPDLMAAGKGITGGYLPIAVTFATEDIYKAFYDDYENLKTFFHGHSYTG
NQLGCAVALENLALFESENIVEQVAEKSKKLHFLLQDLHALPHVGDIRQLGFMCGAELVRSKETKEPYPADRRIGYKVSL
KMRELGMLTRPLGDVIAFLPPLASTAEELSEMVAIMKQAIHEVTSLED
>Mature_447_residues
THDLIEKSKKHLWLPFTQMKDYDENPLIIESGTGIKVKDINGKEYYDGFSSVWLNVHGHRKKELDDAIKKQLGKIAHSTL
LGMTNVPATQLAETLIDISPKKLTRVFYSDSGAEAMEIALKMAFQYWKNIGKPEKQKFIAMKNGYHGDTIGAVSVGSIEL
FHHVYGPLMFESYKAPIPYVYRSESGDPDECRDQCLRELAQLLEEHHEEIAALSIESMVQGASGMIVMPEGYLAGVRELC
TTYDVLMIVDEVATGFGRTGKMFACEHENVQPDLMAAGKGITGGYLPIAVTFATEDIYKAFYDDYENLKTFFHGHSYTGN
QLGCAVALENLALFESENIVEQVAEKSKKLHFLLQDLHALPHVGDIRQLGFMCGAELVRSKETKEPYPADRRIGYKVSLK
MRELGMLTRPLGDVIAFLPPLASTAEELSEMVAIMKQAIHEVTSLED

Specific function: Catalyzes the formation of the intermediate 7,8- diaminopelargonic acid (DAPA) from 7-keto-8-aminopelargonic acid (KAPA) with lysine as the amino donor. B.subtilis does not utilize S-adenosylmethionine (SAM), but instead utilizes only L-lysine as the amin

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=427, Percent_Identity=31.3817330210773, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI284507298, Length=339, Percent_Identity=34.2182890855457, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI13994255, Length=460, Percent_Identity=26.5217391304348, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI226442705, Length=414, Percent_Identity=28.9855072463768, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI37574042, Length=415, Percent_Identity=28.9156626506024, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI24119277, Length=431, Percent_Identity=25.754060324826, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI226442709, Length=365, Percent_Identity=29.8630136986301, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI1786991, Length=446, Percent_Identity=34.5291479820628, Blast_Score=251, Evalue=7e-68,
Organism=Escherichia coli, GI145693181, Length=454, Percent_Identity=30.3964757709251, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1788044, Length=413, Percent_Identity=29.5399515738499, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789759, Length=424, Percent_Identity=29.2452830188679, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1789016, Length=427, Percent_Identity=27.1662763466042, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1787560, Length=418, Percent_Identity=28.2296650717703, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1786349, Length=339, Percent_Identity=24.188790560472, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25144271, Length=426, Percent_Identity=26.7605633802817, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI32564660, Length=446, Percent_Identity=29.1479820627803, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25144274, Length=303, Percent_Identity=28.7128712871287, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71992977, Length=439, Percent_Identity=24.6013667425968, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324386, Length=475, Percent_Identity=30.1052631578947, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6324432, Length=429, Percent_Identity=30.7692307692308, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6323470, Length=429, Percent_Identity=26.5734265734266, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6321456, Length=306, Percent_Identity=25.4901960784314, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21357415, Length=432, Percent_Identity=29.8611111111111, Blast_Score=165, Evalue=7e-41,
Organism=Drosophila melanogaster, GI161085790, Length=482, Percent_Identity=26.5560165975104, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI28574759, Length=485, Percent_Identity=26.5979381443299, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21356575, Length=442, Percent_Identity=26.4705882352941, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOK_BACSU (P53555)

Other databases:

- EMBL:   U51868
- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   C69594
- RefSeq:   NP_390901.1
- PDB:   3DOD
- PDB:   3DRD
- PDB:   3DU4
- PDBsum:   3DOD
- PDBsum:   3DRD
- PDBsum:   3DU4
- ProteinModelPortal:   P53555
- SMR:   P53555
- EnsemblBacteria:   EBBACT00000000459
- GeneID:   936286
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU30230
- NMPDR:   fig|224308.1.peg.3026
- GenoList:   BSU30230
- GeneTree:   EBGT00070000031850
- HOGENOM:   HBG725944
- OMA:   PLMFESY
- PhylomeDB:   P53555
- ProtClustDB:   PRK06916
- BioCyc:   BSUB:BSU30230-MONOMER
- BRENDA:   2.6.1.62
- GO:   GO:0005737
- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- PANTHER:   PTHR11986:SF8
- TIGRFAMs:   TIGR00508

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: 2.6.1.62

Molecular weight: Translated: 50112; Mature: 49981

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: BINDING 251-251 BINDING 280-280 BINDING 315-315 BINDING 317-317 BINDING 410-410

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHDLIEKSKKHLWLPFTQMKDYDENPLIIESGTGIKVKDINGKEYYDGFSSVWLNVHGH
CCHHHHHHCCCCEECCHHHHCCCCCCCEEEECCCCEEEEECCCCHHHCCHHHEEEEECCC
RKKELDDAIKKQLGKIAHSTLLGMTNVPATQLAETLIDISPKKLTRVFYSDSGAEAMEIA
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHH
LKMAFQYWKNIGKPEKQKFIAMKNGYHGDTIGAVSVGSIELFHHVYGPLMFESYKAPIPY
HHHHHHHHHHCCCCCHHHEEEECCCCCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCCCE
VYRSESGDPDECRDQCLRELAQLLEEHHEEIAALSIESMVQGASGMIVMPEGYLAGVREL
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHH
CTTYDVLMIVDEVATGFGRTGKMFACEHENVQPDLMAAGKGITGGYLPIAVTFATEDIYK
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHCCCCCCCCCEEEEEEEHHHHHHH
AFYDDYENLKTFFHGHSYTGNQLGCAVALENLALFESENIVEQVAEKSKKLHFLLQDLHA
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPHVGDIRQLGFMCGAELVRSKETKEPYPADRRIGYKVSLKMRELGMLTRPLGDVIAFLP
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCHHHHHHHHC
PLASTAEELSEMVAIMKQAIHEVTSLED
CHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
THDLIEKSKKHLWLPFTQMKDYDENPLIIESGTGIKVKDINGKEYYDGFSSVWLNVHGH
CHHHHHHCCCCEECCHHHHCCCCCCCEEEECCCCEEEEECCCCHHHCCHHHEEEEECCC
RKKELDDAIKKQLGKIAHSTLLGMTNVPATQLAETLIDISPKKLTRVFYSDSGAEAMEIA
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHH
LKMAFQYWKNIGKPEKQKFIAMKNGYHGDTIGAVSVGSIELFHHVYGPLMFESYKAPIPY
HHHHHHHHHHCCCCCHHHEEEECCCCCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCCCE
VYRSESGDPDECRDQCLRELAQLLEEHHEEIAALSIESMVQGASGMIVMPEGYLAGVREL
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHH
CTTYDVLMIVDEVATGFGRTGKMFACEHENVQPDLMAAGKGITGGYLPIAVTFATEDIYK
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHCCCCCCCCCEEEEEEEHHHHHHH
AFYDDYENLKTFFHGHSYTGNQLGCAVALENLALFESENIVEQVAEKSKKLHFLLQDLHA
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPHVGDIRQLGFMCGAELVRSKETKEPYPADRRIGYKVSLKMRELGMLTRPLGDVIAFLP
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCHHHHHHHHC
PLASTAEELSEMVAIMKQAIHEVTSLED
CHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; 8-amino-7-oxononanoate

Specific reaction: S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763940; 9387221; 9384377