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The map label for this gene is ytnL

Identifier: 16079981

GI number: 16079981

Start: 2999717

End: 3000967

Strand: Reverse

Name: ytnL

Synonym: BSU29290

Alternate gene names: 16079981

Gene position: 3000967-2999717 (Counterclockwise)

Preceding gene: 16079982

Following gene: 16079980

Centisome position: 71.19

GC content: 45.0

Gene sequence:

>1251_bases
ATGTCTTTGGATTATTGGAGAAATATAGAAGGCTCCTATCCATATCAGACTACAGGAAATGACATCCTGACGTTGAAAGA
GGAGTCTAATCCCGTAAATCTCTCAACGCTAGAAAAGCAGCTCATCGGCATTCGCCGGCACCTGCACCAATATCCTGAAC
TGTCAAAAGAAGAATTTGAAACAACTGCTTTTATAAAAAAATGCCTTAAAGAAAAGGGGATACAGATTCGCCCTACTGCT
CTAAAAACGGGTGTGTTCGCAGACATAGCAGGGGAGTCAGAAGGTCCGGCGATTGCTTTGAGGGCTGATATTGACGCCTT
GCCAATTGAAGAAAAAACAGGATTGCCTTATGCGTCTAAACATAAAGGGATCATGCATGCGTGCGGTCATGATTTTCATA
CGGCAGCGCTTCTTGGTGCGGCCTTTTTATTGAAGGAAAATCAAGACAGTCTGAAAGGAAAGATTCGTTTATTATTTCAG
CCAGCAGAGGAAGCGGGTGCAGGCGCTACAAAAGTGATAGAGGACGGCCAACTGGATGGCATTGATGCGGTTATTGGCCT
TCACAATAAACCGGATATCGCGGTTGGGACTGTAGGGTTAAAAACAGGCCCGCTTATGGCTGCTGTTGACCGATTTAAAG
TAGAGATAGAAGGGAAAGGTGCTCATGCAGCATTGCCGCATAATGGTTTTGATCCAATTATCGGCGCCTCTCAGCTTATT
GTCGCCCTGCAAACGATTGTAAGCAGAAATGTAAACCCGCTGCAAAGCGCGATTTTAACCGTAGGGAAAATCAACGGCGG
CAGCACTTGGAATGTCATTCCGGATACAGTGGTCATTGAGGGCACTGTTCGTACATTTGATTCAGAGGTTCGTAATCAAG
TGAAACAGCGTTTTTTTGCAGTCACAGAGCAGATTTCTGCCGCTTTCTCGTTAAAGGCAAATGTGAAGTGGCATTCCGGA
CCGCCGCCGCTTTGTAATGATGAGGCAATTACGGGGCTGGTGCGAGACGCTGCACATAAAGCGAAACTTCAAGTCATTGA
TCCCGCCCCATCAACCGCGGGGGAAGATTTTGCTTATTATCTTGAGCATATTCCGGGATCCTTTGCGTTTTTTGGAACAG
ACGGTGATCATGATTGGCACCACCCTGCATTTACAATAGATGAAACTGCAATTATCAAAGCCTCTTATTTTTTATATGAA
AGCGCCAAGAGATTGTTAGATTCGAATGAAGAGTCAAAAATATCTGATTGA

Upstream 100 bases:

>100_bases
TTGGGGCGGGCGGTCTTGCTGATAAATTGAACCGATTTATTTCATCTTATGTTGAAGAGTCTGAAGAAAATATATTGGGA
TAGGGAAAGGAGCGGAACAT

Downstream 100 bases:

>100_bases
TATAGATGGGGTGTTCTGATGAAGAAATTAATCGTTTTTGCATTTATTGGTTTATTGTCGCAGTTAATAGACGGTTCGTT
AGGTATGGCTTATGGGGTTA

Product: aminohydrolase

Products: benzoate; glycine

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSLDYWRNIEGSYPYQTTGNDILTLKEESNPVNLSTLEKQLIGIRRHLHQYPELSKEEFETTAFIKKCLKEKGIQIRPTA
LKTGVFADIAGESEGPAIALRADIDALPIEEKTGLPYASKHKGIMHACGHDFHTAALLGAAFLLKENQDSLKGKIRLLFQ
PAEEAGAGATKVIEDGQLDGIDAVIGLHNKPDIAVGTVGLKTGPLMAAVDRFKVEIEGKGAHAALPHNGFDPIIGASQLI
VALQTIVSRNVNPLQSAILTVGKINGGSTWNVIPDTVVIEGTVRTFDSEVRNQVKQRFFAVTEQISAAFSLKANVKWHSG
PPPLCNDEAITGLVRDAAHKAKLQVIDPAPSTAGEDFAYYLEHIPGSFAFFGTDGDHDWHHPAFTIDETAIIKASYFLYE
SAKRLLDSNEESKISD

Sequences:

>Translated_416_residues
MSLDYWRNIEGSYPYQTTGNDILTLKEESNPVNLSTLEKQLIGIRRHLHQYPELSKEEFETTAFIKKCLKEKGIQIRPTA
LKTGVFADIAGESEGPAIALRADIDALPIEEKTGLPYASKHKGIMHACGHDFHTAALLGAAFLLKENQDSLKGKIRLLFQ
PAEEAGAGATKVIEDGQLDGIDAVIGLHNKPDIAVGTVGLKTGPLMAAVDRFKVEIEGKGAHAALPHNGFDPIIGASQLI
VALQTIVSRNVNPLQSAILTVGKINGGSTWNVIPDTVVIEGTVRTFDSEVRNQVKQRFFAVTEQISAAFSLKANVKWHSG
PPPLCNDEAITGLVRDAAHKAKLQVIDPAPSTAGEDFAYYLEHIPGSFAFFGTDGDHDWHHPAFTIDETAIIKASYFLYE
SAKRLLDSNEESKISD
>Mature_415_residues
SLDYWRNIEGSYPYQTTGNDILTLKEESNPVNLSTLEKQLIGIRRHLHQYPELSKEEFETTAFIKKCLKEKGIQIRPTAL
KTGVFADIAGESEGPAIALRADIDALPIEEKTGLPYASKHKGIMHACGHDFHTAALLGAAFLLKENQDSLKGKIRLLFQP
AEEAGAGATKVIEDGQLDGIDAVIGLHNKPDIAVGTVGLKTGPLMAAVDRFKVEIEGKGAHAALPHNGFDPIIGASQLIV
ALQTIVSRNVNPLQSAILTVGKINGGSTWNVIPDTVVIEGTVRTFDSEVRNQVKQRFFAVTEQISAAFSLKANVKWHSGP
PPLCNDEAITGLVRDAAHKAKLQVIDPAPSTAGEDFAYYLEHIPGSFAFFGTDGDHDWHHPAFTIDETAIIKASYFLYES
AKRLLDSNEESKISD

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family

Homologues:

Organism=Escherichia coli, GI87081880, Length=340, Percent_Identity=29.4117647058824, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YTNL_BACSU (O34980)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   E69640
- RefSeq:   NP_390807.1
- ProteinModelPortal:   O34980
- SMR:   O34980
- MEROPS:   M20.015
- EnsemblBacteria:   EBBACT00000001818
- GeneID:   936157
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29290
- NMPDR:   fig|224308.1.peg.2932
- GenoList:   BSU29290
- GeneTree:   EBGT00070000031917
- HOGENOM:   HBG708500
- OMA:   GAYQVIE
- PhylomeDB:   O34980
- BioCyc:   BSUB:BSU29290-MONOMER
- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650
- PIRSF:   PIRSF005962
- TIGRFAMs:   TIGR01891

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: 3.5.1.32

Molecular weight: Translated: 45240; Mature: 45109

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDYWRNIEGSYPYQTTGNDILTLKEESNPVNLSTLEKQLIGIRRHLHQYPELSKEEFE
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
TTAFIKKCLKEKGIQIRPTALKTGVFADIAGESEGPAIALRADIDALPIEEKTGLPYASK
HHHHHHHHHHHCCCEEEEHHHHCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCHH
HKGIMHACGHDFHTAALLGAAFLLKENQDSLKGKIRLLFQPAEEAGAGATKVIEDGQLDG
CCCHHHHCCCCHHHHHHHHHHHHHCCCCHHCCEEEEEEEECCHHHCCCCEEEECCCCCCC
IDAVIGLHNKPDIAVGTVGLKTGPLMAAVDRFKVEIEGKGAHAALPHNGFDPIIGASQLI
CHHEEECCCCCCEEEEEECCCCCCHHHEEEEEEEEEECCCCEEECCCCCCCCCCCHHHHH
VALQTIVSRNVNPLQSAILTVGKINGGSTWNVIPDTVVIEGTVRTFDSEVRNQVKQRFFA
HHHHHHHHCCCCHHHHHHHHEEECCCCCEECCCCCCEEEECHHHHHHHHHHHHHHHHHHH
VTEQISAAFSLKANVKWHSGPPPLCNDEAITGLVRDAAHKAKLQVIDPAPSTAGEDFAYY
HHHHHHHHEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHH
LEHIPGSFAFFGTDGDHDWHHPAFTIDETAIIKASYFLYESAKRLLDSNEESKISD
HHHCCCCEEEEECCCCCCCCCCCEEECCHHEEHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SLDYWRNIEGSYPYQTTGNDILTLKEESNPVNLSTLEKQLIGIRRHLHQYPELSKEEFE
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
TTAFIKKCLKEKGIQIRPTALKTGVFADIAGESEGPAIALRADIDALPIEEKTGLPYASK
HHHHHHHHHHHCCCEEEEHHHHCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCHH
HKGIMHACGHDFHTAALLGAAFLLKENQDSLKGKIRLLFQPAEEAGAGATKVIEDGQLDG
CCCHHHHCCCCHHHHHHHHHHHHHCCCCHHCCEEEEEEEECCHHHCCCCEEEECCCCCCC
IDAVIGLHNKPDIAVGTVGLKTGPLMAAVDRFKVEIEGKGAHAALPHNGFDPIIGASQLI
CHHEEECCCCCCEEEEEECCCCCCHHHEEEEEEEEEECCCCEEECCCCCCCCCCCHHHHH
VALQTIVSRNVNPLQSAILTVGKINGGSTWNVIPDTVVIEGTVRTFDSEVRNQVKQRFFA
HHHHHHHHCCCCHHHHHHHHEEECCCCCEECCCCCCEEEECHHHHHHHHHHHHHHHHHHH
VTEQISAAFSLKANVKWHSGPPPLCNDEAITGLVRDAAHKAKLQVIDPAPSTAGEDFAYY
HHHHHHHHEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHH
LEHIPGSFAFFGTDGDHDWHHPAFTIDETAIIKASYFLYESAKRLLDSNEESKISD
HHHCCCCEEEEECCCCCCCCCCCEEECCHHEEHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: hippurate; H2O

Specific reaction: hippurate + H2O = benzoate + glycine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377