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The map label for this gene is rbfK

Identifier: 16079982

GI number: 16079982

Start: 3000985

End: 3001677

Strand: Reverse

Name: rbfK

Synonym: BSU29300

Alternate gene names: 16079982

Gene position: 3001677-3000985 (Counterclockwise)

Preceding gene: 16079983

Following gene: 16079981

Centisome position: 71.2

GC content: 37.37

Gene sequence:

>693_bases
TTGACGATCATTGCCGGTACGGTTGTGAAAGGAAAACAATTAGGCAGAAAGCTTGGATTCCCCACGGCAAATGTAGATGC
AAAAATACATGGGCTGCGTAATGGAGTTTATGGGGTTCTGGCGACAGTCAATCATCAATTTCATTTAGGGGTTATGAATA
TCGGTGTGAAACCGACGGTGGGCTCTAACCTTGAAAAGACATTGGAGATTTTTTTGTTTGACTTTCATAGAGACATTTAT
GGAGAAAAAATCGAATGCAGCATTCTCTTTAAAATTAGAGAAGAAAGAAGGTTTGATTCTTTGGAGTCTTTAACAAAGCA
AATTAAAAAGGATATTTCGTGCGTTGCAAAACGCTTTGAGCTGATTGGGATTATGGCACCAAACAAAAAAGAAAGCCTTC
TTTCCCATCAGGAGTTAAATCTTCCGGATCTCTGCTTTTACAAGAAATGTAATAACCTATATGGCGTCAACCGAGGCGTA
TACAATGTCATTGATAACTGGTTTTTTGAGTACGGAATTACACAAGTAGCTTACAGGCGCATTTATATTTTATCTTTTTT
AAGCTTTTTGAAAGAAGATAATCCGAAAGTTTCCAGCAAGTATATAAGATTTGGGGCGGGCGGTCTTGCTGATAAATTGA
ACCGATTTATTTCATCTTATGTTGAAGAGTCTGAAGAAAATATATTGGGATAG

Upstream 100 bases:

>100_bases
CTCGGTCTTGGCATTCCGCAGCATCAATCCGTTTTACATTCATCACATCATTAGGTTTCGTCTGATCGAATGCGGAAGCT
GAGAGAGGAGGCTGACTTTT

Downstream 100 bases:

>100_bases
GGAAAGGAGCGGAACATATGTCTTTGGATTATTGGAGAAATATAGAAGGCTCCTATCCATATCAGACTACAGGAAATGAC
ATCCTGACGTTGAAAGAGGA

Product: RNA-binding riboflavin kinase

Products: FAD; Diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTIIAGTVVKGKQLGRKLGFPTANVDAKIHGLRNGVYGVLATVNHQFHLGVMNIGVKPTVGSNLEKTLEIFLFDFHRDIY
GEKIECSILFKIREERRFDSLESLTKQIKKDISCVAKRFELIGIMAPNKKESLLSHQELNLPDLCFYKKCNNLYGVNRGV
YNVIDNWFFEYGITQVAYRRIYILSFLSFLKEDNPKVSSKYIRFGAGGLADKLNRFISSYVEESEENILG

Sequences:

>Translated_230_residues
MTIIAGTVVKGKQLGRKLGFPTANVDAKIHGLRNGVYGVLATVNHQFHLGVMNIGVKPTVGSNLEKTLEIFLFDFHRDIY
GEKIECSILFKIREERRFDSLESLTKQIKKDISCVAKRFELIGIMAPNKKESLLSHQELNLPDLCFYKKCNNLYGVNRGV
YNVIDNWFFEYGITQVAYRRIYILSFLSFLKEDNPKVSSKYIRFGAGGLADKLNRFISSYVEESEENILG
>Mature_229_residues
TIIAGTVVKGKQLGRKLGFPTANVDAKIHGLRNGVYGVLATVNHQFHLGVMNIGVKPTVGSNLEKTLEIFLFDFHRDIYG
EKIECSILFKIREERRFDSLESLTKQIKKDISCVAKRFELIGIMAPNKKESLLSHQELNLPDLCFYKKCNNLYGVNRGVY
NVIDNWFFEYGITQVAYRRIYILSFLSFLKEDNPKVSSKYIRFGAGGLADKLNRFISSYVEESEENILG

Specific function: Regulatory protein involved in riboflavin biosynthesis

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786208, Length=110, Percent_Identity=42.7272727272727, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIBR_BACSU (P94465)

Other databases:

- EMBL:   Y09721
- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   G69692
- RefSeq:   NP_390808.1
- ProteinModelPortal:   P94465
- SMR:   P94465
- EnsemblBacteria:   EBBACT00000001087
- GeneID:   937367
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29300
- NMPDR:   fig|224308.1.peg.2933
- GenoList:   BSU29300
- GeneTree:   EBGT00050000002860
- ProtClustDB:   CLSK2752477
- BioCyc:   BSUB:BSU29300-MONOMER
- InterPro:   IPR015865
- Gene3D:   G3DSA:2.40.30.30
- PANTHER:   PTHR22749
- SMART:   SM00904

Pfam domain/function: PF01687 Flavokinase; SSF82114 Riboflavin_kinase

EC number: 2.7.1.26; 2.7.7.2 [C]

Molecular weight: Translated: 26230; Mature: 26099

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIAGTVVKGKQLGRKLGFPTANVDAKIHGLRNGVYGVLATVNHQFHLGVMNIGVKPTV
CEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHCCEEEEEEEECCCCCCC
GSNLEKTLEIFLFDFHRDIYGEKIECSILFKIREERRFDSLESLTKQIKKDISCVAKRFE
CCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIGIMAPNKKESLLSHQELNLPDLCFYKKCNNLYGVNRGVYNVIDNWFFEYGITQVAYRR
HHEEECCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IYILSFLSFLKEDNPKVSSKYIRFGAGGLADKLNRFISSYVEESEENILG
HHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIIAGTVVKGKQLGRKLGFPTANVDAKIHGLRNGVYGVLATVNHQFHLGVMNIGVKPTV
EEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHCCEEEEEEEECCCCCCC
GSNLEKTLEIFLFDFHRDIYGEKIECSILFKIREERRFDSLESLTKQIKKDISCVAKRFE
CCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIGIMAPNKKESLLSHQELNLPDLCFYKKCNNLYGVNRGVYNVIDNWFFEYGITQVAYRR
HHEEECCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IYILSFLSFLKEDNPKVSSKYIRFGAGGLADKLNRFISSYVEESEENILG
HHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; FMN; H+ [C]

Specific reaction: ATP + FMN + H+ --> FAD + Diphosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377