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The map label for this gene is lysC

Identifier: 16079899

GI number: 16079899

Start: 2909520

End: 2910746

Strand: Reverse

Name: lysC

Synonym: BSU28470

Alternate gene names: 16079899

Gene position: 2910746-2909520 (Counterclockwise)

Preceding gene: 255767657

Following gene: 16079897

Centisome position: 69.05

GC content: 45.07

Gene sequence:

>1227_bases
ATGGGTCTTATTGTACAAAAATTCGGAGGCACTTCCGTCGGCTCAGTCGAAAAAATTCAAAATGCGGCAAACCGCGCAAT
TGCAGAAAAACAGAAAGGCCATCAAGTCGTTGTCGTCGTTTCAGCAATGGGAAAATCCACTGACGAATTGGTCAGCCTTG
CAAAAGCTATTTCTGACCAGCCGAGCAAACGCGAAATGGATATGCTGCTGGCGACAGGCGAGCAGGTCACGATTTCACTC
CTTTCAATGGCATTGCAGGAAAAAGGCTATGACGCTGTGTCTTATACTGGCTGGCAGGCGGGAATCCGTACGGAGGCCAT
TCACGGAAACGCCAGAATTACAGATATCGACACTTCGGTTTTAGCAGACCAGCTTGAAAAAGGAAAAATTGTCATTGTTG
CAGGATTCCAAGGCATGACAGAGGATTGTGAAATTACGACATTGGGCCGGGGCGGTTCAGATACAACAGCAGTTGCTTTG
GCTGCCGCTTTAAAAGCTGATAAATGTGACATATACACAGACGTGCCAGGCGTGTTTACAACCGATCCGCGCTATGTGAA
ATCAGCGAGAAAACTTGAGGGCATCTCATACGATGAAATGCTTGAGCTTGCCAATTTAGGCGCCGGTGTTCTTCATCCGA
GAGCAGTTGAGTTCGCGAAAAATTACCAAGTGCCGTTAGAAGTGCGTTCAAGTACAGAAACCGAAGCGGGAACATTAATT
GAGGAGGAATCATCCATGGAACAGAATTTAATTGTCAGAGGCATTGCATTTGAAGATCAAATCACAAGAGTAACCATTTA
CGGGCTGACTAGCGGCCTGACAACTTTGTCTACTATTTTTACAACACTTGCCAAAAGAAACATAAACGTGGATATCATTA
TCCAAACGCAGGCCGAGGACAAGACTGGAATTTCCTTCTCTGTCAAAACAGAAGATGCAGACCAAACCGTTGCGGTGCTT
GAAGAGTATAAAGACGCGCTGGAATTTGAGAAAATCGAGACAGAAAGCAAATTGGCTAAAGTATCTATTGTTGGATCCGG
CATGGTCTCAAATCCCGGTGTAGCGGCTGAAATGTTTGCTGTACTGGCGCAAAAAAACATTTTAATCAAAATGGTCAGCA
CATCTGAAATCAAAGTGTCAACAGTCGTAAGCGAAAATGACATGGTGAAAGCAGTCGAGTCGCTTCACGATGCATTTGAG
CTTTCAAAACACCCTTCAGCTGTGTAA

Upstream 100 bases:

>100_bases
TGATAAGCAATGAGAGTATTCCTCTCATTGCTTTTTTTATTGTGGACAAAGCGCTCTTTCTCCTCACCCGCACGAACCAA
AATGTAAAGGGTGGTAATAC

Downstream 100 bases:

>100_bases
TGACAATCAAAAAGGCGGGACTATGCAGTCACCGCCTTTTTGATTACACTGGGTCTTTCATATCCCATTTCACAGTCAGA
ACCACTTTATCGGCTCTCTT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase 2; Aspartokinase II

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MGLIVQKFGGTSVGSVEKIQNAANRAIAEKQKGHQVVVVVSAMGKSTDELVSLAKAISDQPSKREMDMLLATGEQVTISL
LSMALQEKGYDAVSYTGWQAGIRTEAIHGNARITDIDTSVLADQLEKGKIVIVAGFQGMTEDCEITTLGRGGSDTTAVAL
AAALKADKCDIYTDVPGVFTTDPRYVKSARKLEGISYDEMLELANLGAGVLHPRAVEFAKNYQVPLEVRSSTETEAGTLI
EEESSMEQNLIVRGIAFEDQITRVTIYGLTSGLTTLSTIFTTLAKRNINVDIIIQTQAEDKTGISFSVKTEDADQTVAVL
EEYKDALEFEKIETESKLAKVSIVGSGMVSNPGVAAEMFAVLAQKNILIKMVSTSEIKVSTVVSENDMVKAVESLHDAFE
LSKHPSAV

Sequences:

>Translated_408_residues
MGLIVQKFGGTSVGSVEKIQNAANRAIAEKQKGHQVVVVVSAMGKSTDELVSLAKAISDQPSKREMDMLLATGEQVTISL
LSMALQEKGYDAVSYTGWQAGIRTEAIHGNARITDIDTSVLADQLEKGKIVIVAGFQGMTEDCEITTLGRGGSDTTAVAL
AAALKADKCDIYTDVPGVFTTDPRYVKSARKLEGISYDEMLELANLGAGVLHPRAVEFAKNYQVPLEVRSSTETEAGTLI
EEESSMEQNLIVRGIAFEDQITRVTIYGLTSGLTTLSTIFTTLAKRNINVDIIIQTQAEDKTGISFSVKTEDADQTVAVL
EEYKDALEFEKIETESKLAKVSIVGSGMVSNPGVAAEMFAVLAQKNILIKMVSTSEIKVSTVVSENDMVKAVESLHDAFE
LSKHPSAV
>Mature_407_residues
GLIVQKFGGTSVGSVEKIQNAANRAIAEKQKGHQVVVVVSAMGKSTDELVSLAKAISDQPSKREMDMLLATGEQVTISLL
SMALQEKGYDAVSYTGWQAGIRTEAIHGNARITDIDTSVLADQLEKGKIVIVAGFQGMTEDCEITTLGRGGSDTTAVALA
AALKADKCDIYTDVPGVFTTDPRYVKSARKLEGISYDEMLELANLGAGVLHPRAVEFAKNYQVPLEVRSSTETEAGTLIE
EESSMEQNLIVRGIAFEDQITRVTIYGLTSGLTTLSTIFTTLAKRNINVDIIIQTQAEDKTGISFSVKTEDADQTVAVLE
EYKDALEFEKIETESKLAKVSIVGSGMVSNPGVAAEMFAVLAQKNILIKMVSTSEIKVSTVVSENDMVKAVESLHDAFEL
SKHPSAV

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains

Homologues:

Organism=Escherichia coli, GI1786183, Length=338, Percent_Identity=32.2485207100592, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1790455, Length=460, Percent_Identity=28.2608695652174, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1790376, Length=279, Percent_Identity=26.1648745519713, Blast_Score=81, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320893, Length=375, Percent_Identity=26.1333333333333, Blast_Score=113, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AK2_BACSU (P08495)

Other databases:

- EMBL:   J03294
- EMBL:   J03294
- EMBL:   Z75208
- EMBL:   Z75208
- EMBL:   AL009126
- PIR:   A29314
- RefSeq:   NP_390725.1
- ProteinModelPortal:   P08495
- SMR:   P08495
- EnsemblBacteria:   EBBACT00000001777
- GeneID:   937451
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28470
- NMPDR:   fig|224308.1.peg.2850
- GenoList:   BSU28470
- GeneTree:   EBGT00050000001782
- HOGENOM:   HBG724395
- OMA:   NPSKREM
- PhylomeDB:   P08495
- ProtClustDB:   PRK06635
- BioCyc:   BSUB:BSU28470-MONOMER
- BioCyc:   MetaCyc:MONOMER-6561
- BioCyc:   MetaCyc:MONOMER-6562
- BRENDA:   2.7.2.4
- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042
- Gene3D:   G3DSA:3.40.1160.10
- TIGRFAMs:   TIGR00656
- TIGRFAMs:   TIGR00657

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT; SSF53633 Aa_kinase

EC number: =2.7.2.4

Molecular weight: Translated: 43808; Mature: 43677

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLIVQKFGGTSVGSVEKIQNAANRAIAEKQKGHQVVVVVSAMGKSTDELVSLAKAISDQ
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCC
PSKREMDMLLATGEQVTISLLSMALQEKGYDAVSYTGWQAGIRTEAIHGNARITDIDTSV
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCHHCCCEEEEEECCCEEEECHHHH
LADQLEKGKIVIVAGFQGMTEDCEITTLGRGGSDTTAVALAAALKADKCDIYTDVPGVFT
HHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEECCCCCEE
TDPRYVKSARKLEGISYDEMLELANLGAGVLHPRAVEFAKNYQVPLEVRSSTETEAGTLI
CCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCEE
EEESSMEQNLIVRGIAFEDQITRVTIYGLTSGLTTLSTIFTTLAKRNINVDIIIQTQAED
CCHHHHCCCEEEEEEEECCCEEEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
KTGISFSVKTEDADQTVAVLEEYKDALEFEKIETESKLAKVSIVGSGMVSNPGVAAEMFA
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEECCCCCCCCHHHHHHH
VLAQKNILIKMVSTSEIKVSTVVSENDMVKAVESLHDAFELSKHPSAV
HHHCCCEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GLIVQKFGGTSVGSVEKIQNAANRAIAEKQKGHQVVVVVSAMGKSTDELVSLAKAISDQ
CEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCC
PSKREMDMLLATGEQVTISLLSMALQEKGYDAVSYTGWQAGIRTEAIHGNARITDIDTSV
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCHHCCCEEEEEECCCEEEECHHHH
LADQLEKGKIVIVAGFQGMTEDCEITTLGRGGSDTTAVALAAALKADKCDIYTDVPGVFT
HHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEECCCCCEE
TDPRYVKSARKLEGISYDEMLELANLGAGVLHPRAVEFAKNYQVPLEVRSSTETEAGTLI
CCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCEE
EEESSMEQNLIVRGIAFEDQITRVTIYGLTSGLTTLSTIFTTLAKRNINVDIIIQTQAED
CCHHHHCCCEEEEEEEECCCEEEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
KTGISFSVKTEDADQTVAVLEEYKDALEFEKIETESKLAKVSIVGSGMVSNPGVAAEMFA
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEECCCCCCCCHHHHHHH
VLAQKNILIKMVSTSEIKVSTVVSENDMVKAVESLHDAFELSKHPSAV
HHHCCCEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036830; 8969504; 9384377; 2837491; 2168395