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The map label for this gene is leuA

Identifier: 16079880

GI number: 16079880

Start: 2892138

End: 2893694

Strand: Reverse

Name: leuA

Synonym: BSU28280

Alternate gene names: 16079880

Gene position: 2893694-2892138 (Counterclockwise)

Preceding gene: 16079881

Following gene: 255767650

Centisome position: 68.64

GC content: 44.44

Gene sequence:

>1557_bases
TTGCGCAAAATTAATTTTTTCGATACGACGCTTCGTGATGGTGAACAGTCCCCTGGAGTGAACTTGAATACACAGGAGAA
ACTTGCCATAGCTAAGCAGCTCGAAAGACTCGGGGCAGACATCATTGAAGCGGGATTTCCCGCTTCGTCCCGAGGTGACT
TTTTAGCTGTTCAGGAAATCGCAAGAACCATTAAAAATTGTTCAGTAACTGGTCTGGCCCGTTGTGTAAAAGGTGATATT
GATGCTGCTTGGGAAGCGTTAAAGGATGGCGCTCAACCAAGAATTCATGTATTTATCGCGACATCGGACATTCATTTGAA
GCACAAGCTGAAAATGACACGTGAACAAGTCATTGAAAGAGCAGTTGGAATGGTGAAATACGCAAAAGAACGTTTTCCGA
TTGTGCAATGGTCAGCTGAAGATGCCTGCCGCACTGAACTGCCGTTTCTAGCAGAAATCGTCGAGAAAGTGATTGACGCA
GGCGCCAGTGTTATCAATCTTCCGGACACTGTCGGCTACCTGGCCCCGGCGGAATACGGAAATATCTTTAAATATATGAA
AGAAAACGTTCCGAACATTCACAAAGCAAAGCTTTCAGCCCACTGTCATGATGATTTGGGAATGGCAGTCGCAAACTCTC
TTGCTGCGATTGAAAATGGCGCTGATCAAATCGAATGCGCTGTGAACGGGATCGGTGAAAGAGCCGGAAACGCGGCATTA
GAGGAAATTGCCGTAGCCCTCCATACCAGAAAAGATTTCTACCAAGTCGAAACAGGTATTACGCTGAACGAGATTAAGAG
AACAAGTGATTTAGTAAGCAAACTGACAGGCATGGCTGTCCCGCGCAACAAAGCGGTTGTTGGAGATAATGCATTTGCTC
ATGAATCAGGCATCCATCAGGACGGCTTTTTAAAGGAAAAATCGACTTATGAAATTATTTCACCGGAGCTTGTCGGCGTA
ACCGCAGATGCGCTTGTCCTAGGTAAACATTCCGGACGCCACGCATTTAAAGACCGGCTGACTGCTTTAGGATTCCAATT
TGACAGTGAAGAGATTAATAAATTCTTTACGATGTTCAAAGAGTTGACTGAGAAGAAAAAAGAAATCACTGATGAGGATC
TTGTTTCTCTTATTTTAGAAGAAAAAGTAACAGATCGCAAGATTGGGTATGAATTTCTTTCTCTGCAAGTACATTACGGA
ACAAGTCAGGTCCCTACGGCTACTCTATCTTTGAAAAATCAAGAAAACGCACAGCTTATTCAGGAAGCTGCAACTGGAGC
TGGAAGCGTGGAAGCAATCTACAATACGCTTGAGCGCTGCATCGATAAGGATGTGGAGCTCTTAGACTACCGCATTCAGT
CTAATAGAAAAGGCGAAGATGCATTTGCCCAGGTGTATGTAAGAGTTTTGGTGAACGGAAAAGAATCAGCAGGGCGGGGC
ATAGCGCAAGACGTATTAGAAGCATCAGCGAAAGCCTATTTGAACGCAGTAAACCGTCAATTGGTTTTCCAGTCGAATAT
GAGCGGATTGAAAAACCACACAGCTGTCGGATCATAA

Upstream 100 bases:

>100_bases
GCAAGCGAGAACGAACATCAAATCGAAGTAGTGGGAAGAAAGCTTCGTGAAATGATGCCGTTTGTGAAACAAGGCAAGAA
GAAGGAAGCGGTGGTCTCCG

Downstream 100 bases:

>100_bases
AAGAAAGGAGAACGGTTAACTTGAAGAAACGTATTGCTCTATTGCCCGGAGACGGGATCGGCCCTGAAGTATTAGAATCA
GCGACAGACGTACTGAAAAG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MRKINFFDTTLRDGEQSPGVNLNTQEKLAIAKQLERLGADIIEAGFPASSRGDFLAVQEIARTIKNCSVTGLARCVKGDI
DAAWEALKDGAQPRIHVFIATSDIHLKHKLKMTREQVIERAVGMVKYAKERFPIVQWSAEDACRTELPFLAEIVEKVIDA
GASVINLPDTVGYLAPAEYGNIFKYMKENVPNIHKAKLSAHCHDDLGMAVANSLAAIENGADQIECAVNGIGERAGNAAL
EEIAVALHTRKDFYQVETGITLNEIKRTSDLVSKLTGMAVPRNKAVVGDNAFAHESGIHQDGFLKEKSTYEIISPELVGV
TADALVLGKHSGRHAFKDRLTALGFQFDSEEINKFFTMFKELTEKKKEITDEDLVSLILEEKVTDRKIGYEFLSLQVHYG
TSQVPTATLSLKNQENAQLIQEAATGAGSVEAIYNTLERCIDKDVELLDYRIQSNRKGEDAFAQVYVRVLVNGKESAGRG
IAQDVLEASAKAYLNAVNRQLVFQSNMSGLKNHTAVGS

Sequences:

>Translated_518_residues
MRKINFFDTTLRDGEQSPGVNLNTQEKLAIAKQLERLGADIIEAGFPASSRGDFLAVQEIARTIKNCSVTGLARCVKGDI
DAAWEALKDGAQPRIHVFIATSDIHLKHKLKMTREQVIERAVGMVKYAKERFPIVQWSAEDACRTELPFLAEIVEKVIDA
GASVINLPDTVGYLAPAEYGNIFKYMKENVPNIHKAKLSAHCHDDLGMAVANSLAAIENGADQIECAVNGIGERAGNAAL
EEIAVALHTRKDFYQVETGITLNEIKRTSDLVSKLTGMAVPRNKAVVGDNAFAHESGIHQDGFLKEKSTYEIISPELVGV
TADALVLGKHSGRHAFKDRLTALGFQFDSEEINKFFTMFKELTEKKKEITDEDLVSLILEEKVTDRKIGYEFLSLQVHYG
TSQVPTATLSLKNQENAQLIQEAATGAGSVEAIYNTLERCIDKDVELLDYRIQSNRKGEDAFAQVYVRVLVNGKESAGRG
IAQDVLEASAKAYLNAVNRQLVFQSNMSGLKNHTAVGS
>Mature_518_residues
MRKINFFDTTLRDGEQSPGVNLNTQEKLAIAKQLERLGADIIEAGFPASSRGDFLAVQEIARTIKNCSVTGLARCVKGDI
DAAWEALKDGAQPRIHVFIATSDIHLKHKLKMTREQVIERAVGMVKYAKERFPIVQWSAEDACRTELPFLAEIVEKVIDA
GASVINLPDTVGYLAPAEYGNIFKYMKENVPNIHKAKLSAHCHDDLGMAVANSLAAIENGADQIECAVNGIGERAGNAAL
EEIAVALHTRKDFYQVETGITLNEIKRTSDLVSKLTGMAVPRNKAVVGDNAFAHESGIHQDGFLKEKSTYEIISPELVGV
TADALVLGKHSGRHAFKDRLTALGFQFDSEEINKFFTMFKELTEKKKEITDEDLVSLILEEKVTDRKIGYEFLSLQVHYG
TSQVPTATLSLKNQENAQLIQEAATGAGSVEAIYNTLERCIDKDVELLDYRIQSNRKGEDAFAQVYVRVLVNGKESAGRG
IAQDVLEASAKAYLNAVNRQLVFQSNMSGLKNHTAVGS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=497, Percent_Identity=46.2776659959759, Blast_Score=436, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6320071, Length=371, Percent_Identity=28.0323450134771, Blast_Score=142, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6320019, Length=420, Percent_Identity=27.6190476190476, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6324225, Length=561, Percent_Identity=24.777183600713, Blast_Score=127, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6324682, Length=561, Percent_Identity=23.8859180035651, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24582381, Length=280, Percent_Identity=27.5, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_BACSU (P94565)

Other databases:

- EMBL:   Z75208
- EMBL:   AL009126
- PIR:   H69649
- RefSeq:   NP_390706.1
- ProteinModelPortal:   P94565
- SMR:   P94565
- EnsemblBacteria:   EBBACT00000002890
- GeneID:   936316
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28280
- NMPDR:   fig|224308.1.peg.2831
- GenoList:   BSU28280
- GeneTree:   EBGT00050000000890
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- PhylomeDB:   P94565
- ProtClustDB:   PRK00915
- BioCyc:   BSUB:BSU28280-MONOMER
- BRENDA:   2.3.3.13
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 56912; Mature: 56912

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKINFFDTTLRDGEQSPGVNLNTQEKLAIAKQLERLGADIIEAGFPASSRGDFLAVQEI
CCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCHHHHHH
ARTIKNCSVTGLARCVKGDIDAAWEALKDGAQPRIHVFIATSDIHLKHKLKMTREQVIER
HHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEEECCEEHHHHHHHHHHHHHHH
AVGMVKYAKERFPIVQWSAEDACRTELPFLAEIVEKVIDAGASVINLPDTVGYLAPAEYG
HHHHHHHHHHHCCEEEECCCCHHHHCCHHHHHHHHHHHHCCCCEEECCHHHCCCCCHHHH
NIFKYMKENVPNIHKAKLSAHCHDDLGMAVANSLAAIENGADQIECAVNGIGERAGNAAL
HHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHH
EEIAVALHTRKDFYQVETGITLNEIKRTSDLVSKLTGMAVPRNKAVVGDNAFAHESGIHQ
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHCCCCCC
DGFLKEKSTYEIISPELVGVTADALVLGKHSGRHAFKDRLTALGFQFDSEEINKFFTMFK
CCCCCCCCCCEECCCHHCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
ELTEKKKEITDEDLVSLILEEKVTDRKIGYEFLSLQVHYGTSQVPTATLSLKNQENAQLI
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCEEEEEECCCHHHHHH
QEAATGAGSVEAIYNTLERCIDKDVELLDYRIQSNRKGEDAFAQVYVRVLVNGKESAGRG
HHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHCCCH
IAQDVLEASAKAYLNAVNRQLVFQSNMSGLKNHTAVGS
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCC
>Mature Secondary Structure
MRKINFFDTTLRDGEQSPGVNLNTQEKLAIAKQLERLGADIIEAGFPASSRGDFLAVQEI
CCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCHHHHHH
ARTIKNCSVTGLARCVKGDIDAAWEALKDGAQPRIHVFIATSDIHLKHKLKMTREQVIER
HHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEEECCEEHHHHHHHHHHHHHHH
AVGMVKYAKERFPIVQWSAEDACRTELPFLAEIVEKVIDAGASVINLPDTVGYLAPAEYG
HHHHHHHHHHHCCEEEECCCCHHHHCCHHHHHHHHHHHHCCCCEEECCHHHCCCCCHHHH
NIFKYMKENVPNIHKAKLSAHCHDDLGMAVANSLAAIENGADQIECAVNGIGERAGNAAL
HHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHH
EEIAVALHTRKDFYQVETGITLNEIKRTSDLVSKLTGMAVPRNKAVVGDNAFAHESGIHQ
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHCCCCCC
DGFLKEKSTYEIISPELVGVTADALVLGKHSGRHAFKDRLTALGFQFDSEEINKFFTMFK
CCCCCCCCCCEECCCHHCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
ELTEKKKEITDEDLVSLILEEKVTDRKIGYEFLSLQVHYGTSQVPTATLSLKNQENAQLI
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCEEEEEECCCHHHHHH
QEAATGAGSVEAIYNTLERCIDKDVELLDYRIQSNRKGEDAFAQVYVRVLVNGKESAGRG
HHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHCCCH
IAQDVLEASAKAYLNAVNRQLVFQSNMSGLKNHTAVGS
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377