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The map label for this gene is hemB

Identifier: 16079865

GI number: 16079865

Start: 2874202

End: 2875176

Strand: Reverse

Name: hemB

Synonym: BSU28130

Alternate gene names: 16079865

Gene position: 2875176-2874202 (Counterclockwise)

Preceding gene: 16079866

Following gene: 16079864

Centisome position: 68.2

GC content: 45.23

Gene sequence:

>975_bases
ATGAGTCAATCATTTAATAGACACCGCCGCCTGCGGACATCAAAAGCAATGAGAGAAATGGTAAAGGAAACACGTTTGCA
TCCATCAGATTTTATATATCCGATTTTTGTCGTTGAAGGGCTGGAAGGCAAAAAAGCTGTTCCGTCAATGCCTGATGTTC
ACCATGTATCATTAGATTTATTGAAGGATGAAGTAGCGGAGCTGGTCAAACTGGGCATTCAATCTGTTATCGTGTTCGGC
ATCCCGGAAGAAAAAGATGATTGCGGAACACAAGCGTACCATGATCACGGAATTGTCCAAAAAGCCATCACAGAAATTAA
AGAACACTTCCCTGAAATGGTTGTTGTCGCTGACACGTGCCTGTGCGAATATACAGACCACGGCCATTGCGGACTTGTCA
AAGACGGAGTCATTCTCAATGATGAATCGCTGGAGCTTTTGGCGCAGACAGCTGTCAGCCAAGCGAAAGCAGGTGCGGAT
ATCATTGCGCCATCAAACATGATGGACGGATTTGTTACAGTGATCAGAGAAGCACTTGATAAAGAAGGATTCGTCAATAT
TCCCATCATGTCTTACGCTGTTAAATATTCAAGTGAGTTTTACGGTCCGTTCCGTGATGCAGCAAACAGCACACCGCAAT
TCGGAGACCGCAAAACATATCAGATGGACCCTGCCAACCGTATGGAGGCACTCCGCGAAGCACAATCAGATGTTGAGGAA
GGCGCGGACTTTTTGATTGTCAAACCTTCGCTTTCTTATATGGATATCATGCGTGACGTAAAAAATGAGTTTACTTTGCC
GCTCGTCGCTTATAATGTAAGCGGAGAGTATTCAATGGTGAAGGCTGCAGCGCAGAACGGCTGGATCAAAGAAAAAGAAA
TTGTGTTGGAAATTTTGACAAGCATGAAGCGCGCGGGTGCCGACCTGATTATTACGTATCATGCGAAAGACGCAGCGAAA
TGGCTTGCGGAGTAA

Upstream 100 bases:

>100_bases
CCCTTCTGACGTACGGCATCACATCGCATACGCCTGATACATTTACAATAGATGGCATGCTTGAGTTAATGTGCAGCATG
TCAAGAGAGGAAGAGAGAAT

Downstream 100 bases:

>100_bases
TTTTATTCAGTTGACAGGAGTTGAGGCAGATGAGAAGCTATGAAAAATCAAAAACGGCTTTTAAAGAAGCGCAAAAACTC
ATGCCGGGCGGTGTGAACAG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSQSFNRHRRLRTSKAMREMVKETRLHPSDFIYPIFVVEGLEGKKAVPSMPDVHHVSLDLLKDEVAELVKLGIQSVIVFG
IPEEKDDCGTQAYHDHGIVQKAITEIKEHFPEMVVVADTCLCEYTDHGHCGLVKDGVILNDESLELLAQTAVSQAKAGAD
IIAPSNMMDGFVTVIREALDKEGFVNIPIMSYAVKYSSEFYGPFRDAANSTPQFGDRKTYQMDPANRMEALREAQSDVEE
GADFLIVKPSLSYMDIMRDVKNEFTLPLVAYNVSGEYSMVKAAAQNGWIKEKEIVLEILTSMKRAGADLIITYHAKDAAK
WLAE

Sequences:

>Translated_324_residues
MSQSFNRHRRLRTSKAMREMVKETRLHPSDFIYPIFVVEGLEGKKAVPSMPDVHHVSLDLLKDEVAELVKLGIQSVIVFG
IPEEKDDCGTQAYHDHGIVQKAITEIKEHFPEMVVVADTCLCEYTDHGHCGLVKDGVILNDESLELLAQTAVSQAKAGAD
IIAPSNMMDGFVTVIREALDKEGFVNIPIMSYAVKYSSEFYGPFRDAANSTPQFGDRKTYQMDPANRMEALREAQSDVEE
GADFLIVKPSLSYMDIMRDVKNEFTLPLVAYNVSGEYSMVKAAAQNGWIKEKEIVLEILTSMKRAGADLIITYHAKDAAK
WLAE
>Mature_323_residues
SQSFNRHRRLRTSKAMREMVKETRLHPSDFIYPIFVVEGLEGKKAVPSMPDVHHVSLDLLKDEVAELVKLGIQSVIVFGI
PEEKDDCGTQAYHDHGIVQKAITEIKEHFPEMVVVADTCLCEYTDHGHCGLVKDGVILNDESLELLAQTAVSQAKAGADI
IAPSNMMDGFVTVIREALDKEGFVNIPIMSYAVKYSSEFYGPFRDAANSTPQFGDRKTYQMDPANRMEALREAQSDVEEG
ADFLIVKPSLSYMDIMRDVKNEFTLPLVAYNVSGEYSMVKAAAQNGWIKEKEIVLEILTSMKRAGADLIITYHAKDAAKW
LAE

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family

Homologues:

Organism=Homo sapiens, GI189083849, Length=306, Percent_Identity=44.7712418300654, Blast_Score=271, Evalue=5e-73,
Organism=Escherichia coli, GI87081728, Length=320, Percent_Identity=49.0625, Blast_Score=299, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6321398, Length=314, Percent_Identity=44.5859872611465, Blast_Score=258, Evalue=8e-70,
Organism=Drosophila melanogaster, GI21358291, Length=290, Percent_Identity=41.0344827586207, Blast_Score=249, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM2_BACSU (P30950)

Other databases:

- EMBL:   M57676
- EMBL:   AL009126
- PIR:   C42728
- RefSeq:   NP_390691.1
- ProteinModelPortal:   P30950
- SMR:   P30950
- EnsemblBacteria:   EBBACT00000003067
- GeneID:   936972
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28130
- NMPDR:   fig|224308.1.peg.2816
- GenoList:   BSU28130
- GeneTree:   EBGT00050000002047
- HOGENOM:   HBG285270
- OMA:   ADLCFCE
- PhylomeDB:   P30950
- ProtClustDB:   PRK09283
- BioCyc:   BSUB:BSU28130-MONOMER
- BRENDA:   4.2.1.24
- InterPro:   IPR001731
- InterPro:   IPR013785
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11458
- PIRSF:   PIRSF001415
- PRINTS:   PR00144

Pfam domain/function: PF00490 ALAD

EC number: =4.2.1.24

Molecular weight: Translated: 36210; Mature: 36079

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: ACT_SITE 195-195 ACT_SITE 248-248 BINDING 205-205 BINDING 217-217 BINDING 274-274 BINDING 313-313

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSFNRHRRLRTSKAMREMVKETRLHPSDFIYPIFVVEGLEGKKAVPSMPDVHHVSLDL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHH
LKDEVAELVKLGIQSVIVFGIPEEKDDCGTQAYHDHGIVQKAITEIKEHFPEMVVVADTC
HHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHH
LCEYTDHGHCGLVKDGVILNDESLELLAQTAVSQAKAGADIIAPSNMMDGFVTVIREALD
HHCCCCCCCCCEEECCEEECCHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHC
KEGFVNIPIMSYAVKYSSEFYGPFRDAANSTPQFGDRKTYQMDPANRMEALREAQSDVEE
CCCCEECHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHC
GADFLIVKPSLSYMDIMRDVKNEFTLPLVAYNVSGEYSMVKAAAQNGWIKEKEIVLEILT
CCCEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHH
SMKRAGADLIITYHAKDAAKWLAE
HHHHCCCCEEEEEECHHHHHHHCC
>Mature Secondary Structure 
SQSFNRHRRLRTSKAMREMVKETRLHPSDFIYPIFVVEGLEGKKAVPSMPDVHHVSLDL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHH
LKDEVAELVKLGIQSVIVFGIPEEKDDCGTQAYHDHGIVQKAITEIKEHFPEMVVVADTC
HHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHH
LCEYTDHGHCGLVKDGVILNDESLELLAQTAVSQAKAGADIIAPSNMMDGFVTVIREALD
HHCCCCCCCCCEEECCEEECCHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHC
KEGFVNIPIMSYAVKYSSEFYGPFRDAANSTPQFGDRKTYQMDPANRMEALREAQSDVEE
CCCCEECHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHC
GADFLIVKPSLSYMDIMRDVKNEFTLPLVAYNVSGEYSMVKAAAQNGWIKEKEIVLEILT
CCCEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHH
SMKRAGADLIITYHAKDAAKWLAE
HHHHCCCCEEEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1672867; 9384377