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The map label for this gene is hemL

Identifier: 16079864

GI number: 16079864

Start: 2872880

End: 2874172

Strand: Reverse

Name: hemL

Synonym: BSU28120

Alternate gene names: 16079864

Gene position: 2874172-2872880 (Counterclockwise)

Preceding gene: 16079865

Following gene: 16079863

Centisome position: 68.18

GC content: 47.41

Gene sequence:

>1293_bases
ATGAGAAGCTATGAAAAATCAAAAACGGCTTTTAAAGAAGCGCAAAAACTCATGCCGGGCGGTGTGAACAGTCCCGTTCG
CGCATTTAAATCGGTAGACATGGACCCGATTTTTATGGAGCGCGGAAAAGGCTCGAAAATCTTTGATATTGACGGGAATG
AATATATTGACTACGTCTTGTCATGGGGGCCTTTAATTTTAGGGCATACAAATGACCGCGTCGTAGAAAGCCTCAAAAAA
GTGGCTGAATACGGGACAAGCTTTGGTGCTCCGACTGAAGTAGAAAATGAACTGGCTAAGCTCGTCATTGATCGTGTGCC
ATCTGTAGAAATTGTACGAATGGTAAGCTCCGGAACAGAGGCTACAATGAGTGCCCTCCGTTTGGCAAGGGGCTATACGG
GCCGCAACAAGATTTTAAAATTTGAGGGCTGCTACCACGGACACGGCGATTCTCTCTTGATTAAAGCTGGTTCAGGTGTT
GCCACTCTCGGTCTGCCTGACAGCCCGGGGGTGCCTGAAGGCATTGCGAAAAACACCATCACCGTTCCGTACAATGATTT
AGAAAGTGTAAAGCTTGCTTTCCAGCAATTCGGTGAAGACATTGCGGGAGTCATTGTAGAGCCAGTTGCCGGAAATATGG
GTGTTGTTCCGCCGCAAGAAGGTTTCCTTCAGGGTCTGCGTGATATCACTGAGCAGTACGGCTCCCTGCTGATTTTTGAT
GAAGTGATGACTGGCTTCCGGGTCGATTATAACTGCGCTCAAGGCTACTTCGGCGTAACGCCTGATCTGACTTGTTTAGG
AAAAGTAATCGGGGGCGGACTTCCTGTCGGCGCTTATGGCGGAAAGGCAGAAATCATGGAGCAGATCGCTCCAAGCGGTC
CGATCTATCAAGCTGGTACATTGTCAGGCAACCCGCTTGCGATGACGGCTGGCTTAGAGACATTGAAACAGCTGACACCT
GAATCCTACAAGAATTTCATCAAAAAAGGCGACAGACTGGAAGAAGGAATTTCAAAAACCGCCGGGGCTCATGGCATTCC
GCATACCTTTAACCGTGCAGGTTCGATGATCGGTTTCTTCTTTACAAACGAACCAGTCATCAATTATGAAACAGCGAAAT
CATCTGATTTGAAGCTGTTCGCAAGCTATTATAAAGGGATGGCAAATGAAGGGGTATTCCTTCCGCCATCACAATTCGAA
GGTCTTTTCCTCTCAACGGCCCATACTGATGAAGATATTGAAAACACAATCCAGGCAGCTGAGAAAGTATTTGCTGAGAT
CAGCCGCAGATAA

Upstream 100 bases:

>100_bases
TGAAGCGCGCGGGTGCCGACCTGATTATTACGTATCATGCGAAAGACGCAGCGAAATGGCTTGCGGAGTAATTTTATTCA
GTTGACAGGAGTTGAGGCAG

Downstream 100 bases:

>100_bases
GAGTGAAAACCGGTATCAAGGACTCCTTGTGCCGGTTTTTTCGTGCTCTCCCACTCATATTTTCTCCAGTTCATACATAC
ACCTTTAGTGACATCTAATT

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MRSYEKSKTAFKEAQKLMPGGVNSPVRAFKSVDMDPIFMERGKGSKIFDIDGNEYIDYVLSWGPLILGHTNDRVVESLKK
VAEYGTSFGAPTEVENELAKLVIDRVPSVEIVRMVSSGTEATMSALRLARGYTGRNKILKFEGCYHGHGDSLLIKAGSGV
ATLGLPDSPGVPEGIAKNTITVPYNDLESVKLAFQQFGEDIAGVIVEPVAGNMGVVPPQEGFLQGLRDITEQYGSLLIFD
EVMTGFRVDYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKAEIMEQIAPSGPIYQAGTLSGNPLAMTAGLETLKQLTP
ESYKNFIKKGDRLEEGISKTAGAHGIPHTFNRAGSMIGFFFTNEPVINYETAKSSDLKLFASYYKGMANEGVFLPPSQFE
GLFLSTAHTDEDIENTIQAAEKVFAEISRR

Sequences:

>Translated_430_residues
MRSYEKSKTAFKEAQKLMPGGVNSPVRAFKSVDMDPIFMERGKGSKIFDIDGNEYIDYVLSWGPLILGHTNDRVVESLKK
VAEYGTSFGAPTEVENELAKLVIDRVPSVEIVRMVSSGTEATMSALRLARGYTGRNKILKFEGCYHGHGDSLLIKAGSGV
ATLGLPDSPGVPEGIAKNTITVPYNDLESVKLAFQQFGEDIAGVIVEPVAGNMGVVPPQEGFLQGLRDITEQYGSLLIFD
EVMTGFRVDYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKAEIMEQIAPSGPIYQAGTLSGNPLAMTAGLETLKQLTP
ESYKNFIKKGDRLEEGISKTAGAHGIPHTFNRAGSMIGFFFTNEPVINYETAKSSDLKLFASYYKGMANEGVFLPPSQFE
GLFLSTAHTDEDIENTIQAAEKVFAEISRR
>Mature_430_residues
MRSYEKSKTAFKEAQKLMPGGVNSPVRAFKSVDMDPIFMERGKGSKIFDIDGNEYIDYVLSWGPLILGHTNDRVVESLKK
VAEYGTSFGAPTEVENELAKLVIDRVPSVEIVRMVSSGTEATMSALRLARGYTGRNKILKFEGCYHGHGDSLLIKAGSGV
ATLGLPDSPGVPEGIAKNTITVPYNDLESVKLAFQQFGEDIAGVIVEPVAGNMGVVPPQEGFLQGLRDITEQYGSLLIFD
EVMTGFRVDYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKAEIMEQIAPSGPIYQAGTLSGNPLAMTAGLETLKQLTP
ESYKNFIKKGDRLEEGISKTAGAHGIPHTFNRAGSMIGFFFTNEPVINYETAKSSDLKLFASYYKGMANEGVFLPPSQFE
GLFLSTAHTDEDIENTIQAAEKVFAEISRR

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=318, Percent_Identity=30.8176100628931, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI13994255, Length=325, Percent_Identity=24.9230769230769, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI284507298, Length=230, Percent_Identity=32.6086956521739, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI24119277, Length=311, Percent_Identity=26.3665594855305, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1786349, Length=424, Percent_Identity=54.9528301886792, Blast_Score=490, Evalue=1e-140,
Organism=Escherichia coli, GI1789016, Length=334, Percent_Identity=31.7365269461078, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789759, Length=328, Percent_Identity=30.7926829268293, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1788044, Length=361, Percent_Identity=31.3019390581717, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI145693181, Length=323, Percent_Identity=30.3405572755418, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787560, Length=361, Percent_Identity=28.2548476454294, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1786991, Length=286, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25144271, Length=296, Percent_Identity=29.0540540540541, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI71992977, Length=346, Percent_Identity=26.878612716763, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144274, Length=143, Percent_Identity=37.0629370629371, Blast_Score=85, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323470, Length=303, Percent_Identity=28.0528052805281, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324432, Length=357, Percent_Identity=26.3305322128852, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357415, Length=322, Percent_Identity=26.7080745341615, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_BACSU (P30949)

Other databases:

- EMBL:   M57676
- EMBL:   AL009126
- PIR:   D42728
- RefSeq:   NP_390690.1
- PDB:   3BS8
- PDBsum:   3BS8
- ProteinModelPortal:   P30949
- SMR:   P30949
- EnsemblBacteria:   EBBACT00000001255
- GeneID:   937490
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28120
- NMPDR:   fig|224308.1.peg.2815
- GenoList:   BSU28120
- GeneTree:   EBGT00070000031737
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- PhylomeDB:   P30949
- ProtClustDB:   PRK00062
- BioCyc:   BSUB:BSU28120-MONOMER
- BRENDA:   5.4.3.8
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 46449; Mature: 46449

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSYEKSKTAFKEAQKLMPGGVNSPVRAFKSVDMDPIFMERGKGSKIFDIDGNEYIDYVL
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHCCCCCCEEEECCCCHHHHHHH
SWGPLILGHTNDRVVESLKKVAEYGTSFGAPTEVENELAKLVIDRVPSVEIVRMVSSGTE
HCCCEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHH
ATMSALRLARGYTGRNKILKFEGCYHGHGDSLLIKAGSGVATLGLPDSPGVPEGIAKNTI
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCEE
TVPYNDLESVKLAFQQFGEDIAGVIVEPVAGNMGVVPPQEGFLQGLRDITEQYGSLLIFD
ECCCCHHHHHHHHHHHHCHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEH
EVMTGFRVDYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKAEIMEQIAPSGPIYQAGT
HHHHCCEECEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCC
LSGNPLAMTAGLETLKQLTPESYKNFIKKGDRLEEGISKTAGAHGIPHTFNRAGSMIGFF
CCCCCEEEHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHCCCEEEEE
FTNEPVINYETAKSSDLKLFASYYKGMANEGVFLPPSQFEGLFLSTAHTDEDIENTIQAA
ECCCCEEEECCCCCCHHHHHHHHHHCCCCCCEECCCCCCCCEEEECCCCCHHHHHHHHHH
EKVFAEISRR
HHHHHHHHCC
>Mature Secondary Structure
MRSYEKSKTAFKEAQKLMPGGVNSPVRAFKSVDMDPIFMERGKGSKIFDIDGNEYIDYVL
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHCCCCCCEEEECCCCHHHHHHH
SWGPLILGHTNDRVVESLKKVAEYGTSFGAPTEVENELAKLVIDRVPSVEIVRMVSSGTE
HCCCEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHH
ATMSALRLARGYTGRNKILKFEGCYHGHGDSLLIKAGSGVATLGLPDSPGVPEGIAKNTI
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCEE
TVPYNDLESVKLAFQQFGEDIAGVIVEPVAGNMGVVPPQEGFLQGLRDITEQYGSLLIFD
ECCCCHHHHHHHHHHHHCHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEH
EVMTGFRVDYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKAEIMEQIAPSGPIYQAGT
HHHHCCEECEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCC
LSGNPLAMTAGLETLKQLTPESYKNFIKKGDRLEEGISKTAGAHGIPHTFNRAGSMIGFF
CCCCCEEEHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHCCCEEEEE
FTNEPVINYETAKSSDLKLFASYYKGMANEGVFLPPSQFEGLFLSTAHTDEDIENTIQAA
ECCCCEEEECCCCCCHHHHHHHHHHCCCCCCEECCCCCCCCEEEECCCCCHHHHHHHHHH
EKVFAEISRR
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1672867; 9384377