Definition Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession NC_000964
Length 4,215,606

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The map label for this gene is yqeC

Identifier: 16079626

GI number: 16079626

Start: 2650487

End: 2651380

Strand: Reverse

Name: yqeC

Synonym: BSU25730

Alternate gene names: 16079626

Gene position: 2651380-2650487 (Counterclockwise)

Preceding gene: 16079628

Following gene: 16079624

Centisome position: 62.89

GC content: 45.19

Gene sequence:

>894_bases
ATGAAAATCGGATTAATCGGCTTAGGGAAAATGGGCATAAACATAGGAAAGCAATTCATTGACCGCAATCATCAAGTGGT
TGGATATGATGTAAATCAAGCGGCTGTTGATGAACTGAAAGCATACGGGGCTGAGGGGACAACAAATCTTAAAGAGTTCA
TTTCTTTATTACACCCGCCGCGCATCCTATGGGTTATGGTACCGCATGGCATTGTCGATGCTGTTTTGCGTGATGTATCA
CCTTTATTAAGTAAAGGCGATATGATTATCGAAGCTGGAAACTCTCATTATAAAGAATCAATTCGCCGCTACAATCAAAT
GAAAGAAGCGGGTATCCATTATCTCGATGCCGGAACGTCGGGCGGAATGGAAGGCGCACGGCATGGAGCCTGTTTTATGG
TGGGGGGCGATCATGAAGCATGGGAGATTGTGGAACCGCTTTTTCGTGATACTGCCGTCGAAAATGGTTATCTATACGCT
GGTGAAGCGGGAAGCGGACATTTTTTAAAAATGATCCATAATGGAATTGAGTATGGAATGATGGCCGCGATTGGTGAAGG
ATTCGAAGTGCTTGAAAACAGCCAATTTGATTTTGACTACGAGAAGGTTGCCAGAGTGTGGAATCATGGTTCTGTCATTC
GTTCTTGGCTTATGGGACTGACTGAGCGAGCTTTCGCTAAGGATGCGAAACTGGATCAGATCAAGGGCATTATGCATTCC
TCAGGTGAAGGAAAATGGACGGTGGAAACCGCGCTTGATCTGCAGACGGCAACGCCGGTCATCGCCATGTCACTAATGAT
GAGATACCGTTCTTTAACAGACGATACGTTTACAGGAAAGGTGGTTGCTGCGCTTCGAAATGAATTTGGAGGCCATGCGA
CAGAAAAAAAGTAA

Upstream 100 bases:

>100_bases
GCGCAGACGATGTAAGCCCCATTTGTTTTCTGCGTATCACAATACAATCCATAGGGAACATAAAGAGTAAAGGCTTTTTA
GACAATCAGGAGGAACAGAT

Downstream 100 bases:

>100_bases
GGGGGCCAACAAGCCTCCTTATTTTTTTGGCACCTTCAGAAGATGAGGGAACTTTTTATAAATGACCAGCATGATGATCA
GAATATATGTACCGTAAATG

Product: 6-phosphogluconate dehydrogenase-like protein

Products: D-ribulose 5-phosphate; CO2; NADPH

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKIGLIGLGKMGINIGKQFIDRNHQVVGYDVNQAAVDELKAYGAEGTTNLKEFISLLHPPRILWVMVPHGIVDAVLRDVS
PLLSKGDMIIEAGNSHYKESIRRYNQMKEAGIHYLDAGTSGGMEGARHGACFMVGGDHEAWEIVEPLFRDTAVENGYLYA
GEAGSGHFLKMIHNGIEYGMMAAIGEGFEVLENSQFDFDYEKVARVWNHGSVIRSWLMGLTERAFAKDAKLDQIKGIMHS
SGEGKWTVETALDLQTATPVIAMSLMMRYRSLTDDTFTGKVVAALRNEFGGHATEKK

Sequences:

>Translated_297_residues
MKIGLIGLGKMGINIGKQFIDRNHQVVGYDVNQAAVDELKAYGAEGTTNLKEFISLLHPPRILWVMVPHGIVDAVLRDVS
PLLSKGDMIIEAGNSHYKESIRRYNQMKEAGIHYLDAGTSGGMEGARHGACFMVGGDHEAWEIVEPLFRDTAVENGYLYA
GEAGSGHFLKMIHNGIEYGMMAAIGEGFEVLENSQFDFDYEKVARVWNHGSVIRSWLMGLTERAFAKDAKLDQIKGIMHS
SGEGKWTVETALDLQTATPVIAMSLMMRYRSLTDDTFTGKVVAALRNEFGGHATEKK
>Mature_297_residues
MKIGLIGLGKMGINIGKQFIDRNHQVVGYDVNQAAVDELKAYGAEGTTNLKEFISLLHPPRILWVMVPHGIVDAVLRDVS
PLLSKGDMIIEAGNSHYKESIRRYNQMKEAGIHYLDAGTSGGMEGARHGACFMVGGDHEAWEIVEPLFRDTAVENGYLYA
GEAGSGHFLKMIHNGIEYGMMAAIGEGFEVLENSQFDFDYEKVARVWNHGSVIRSWLMGLTERAFAKDAKLDQIKGIMHS
SGEGKWTVETALDLQTATPVIAMSLMMRYRSLTDDTFTGKVVAALRNEFGGHATEKK

Specific function: May act as NAD-dependent 6-P-gluconate dehydrogenase

COG id: COG1023

COG function: function code G; Predicted 6-phosphogluconate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 6-phosphogluconate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI40068518, Length=292, Percent_Identity=33.9041095890411, Blast_Score=132, Evalue=5e-31,
Organism=Escherichia coli, GI1788341, Length=292, Percent_Identity=33.2191780821918, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1786719, Length=153, Percent_Identity=34.640522875817, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17542558, Length=291, Percent_Identity=32.9896907216495, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321695, Length=294, Percent_Identity=35.0340136054422, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6321977, Length=307, Percent_Identity=36.1563517915309, Blast_Score=156, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24639279, Length=289, Percent_Identity=33.5640138408304, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YQEC_BACSU (P54448)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   G69950
- RefSeq:   NP_390450.1
- ProteinModelPortal:   P54448
- SMR:   P54448
- EnsemblBacteria:   EBBACT00000000836
- GeneID:   937809
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25730
- NMPDR:   fig|224308.1.peg.2575
- GenoList:   BSU25730
- GeneTree:   EBGT00050000001992
- HOGENOM:   HBG286913
- OMA:   VWMMVPA
- PhylomeDB:   P54448
- ProtClustDB:   PRK09599
- BioCyc:   BSUB:BSU25730-MONOMER
- InterPro:   IPR008927
- InterPro:   IPR004849
- InterPro:   IPR006114
- InterPro:   IPR006115
- InterPro:   IPR006184
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PRINTS:   PR00076
- TIGRFAMs:   TIGR00872

Pfam domain/function: PF00393 6PGD; PF03446 NAD_binding_2; SSF48179 6DGDH_C_like

EC number: 1.1.1.44

Molecular weight: Translated: 32770; Mature: 32770

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00461 6PGD

Important sites: ACT_SITE 170-170 ACT_SITE 177-177 BINDING 94-94 BINDING 94-94 BINDING 178-178 BINDING 268-268

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MKIGLIGLGKMGINIGKQFIDRNHQVVGYDVNQAAVDELKAYGAEGTTNLKEFISLLHPP
CEEEEEECCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHCCC
RILWVMVPHGIVDAVLRDVSPLLSKGDMIIEAGNSHYKESIRRYNQMKEAGIHYLDAGTS
EEEEEECCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCCEEEECCCC
GGMEGARHGACFMVGGDHEAWEIVEPLFRDTAVENGYLYAGEAGSGHFLKMIHNGIEYGM
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHCCHHHHH
MAAIGEGFEVLENSQFDFDYEKVARVWNHGSVIRSWLMGLTERAFAKDAKLDQIKGIMHS
HHHHCCCHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
SGEGKWTVETALDLQTATPVIAMSLMMRYRSLTDDTFTGKVVAALRNEFGGHATEKK
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKIGLIGLGKMGINIGKQFIDRNHQVVGYDVNQAAVDELKAYGAEGTTNLKEFISLLHPP
CEEEEEECCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHCCC
RILWVMVPHGIVDAVLRDVSPLLSKGDMIIEAGNSHYKESIRRYNQMKEAGIHYLDAGTS
EEEEEECCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCCEEEECCCC
GGMEGARHGACFMVGGDHEAWEIVEPLFRDTAVENGYLYAGEAGSGHFLKMIHNGIEYGM
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHCCHHHHH
MAAIGEGFEVLENSQFDFDYEKVARVWNHGSVIRSWLMGLTERAFAKDAKLDQIKGIMHS
HHHHCCCHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
SGEGKWTVETALDLQTATPVIAMSLMMRYRSLTDDTFTGKVVAALRNEFGGHATEKK
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {6-phosphogluconate}} 0.01 {6-phosphogluconate}} [C]

Substrates: 6-phospho-D-gluconate; NADP+

Specific reaction: 6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377