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The map label for this gene is cwlH

Identifier: 16079624

GI number: 16079624

Start: 2648903

End: 2649655

Strand: Reverse

Name: cwlH

Synonym: BSU25710

Alternate gene names: 16079624

Gene position: 2649655-2648903 (Counterclockwise)

Preceding gene: 16079626

Following gene: 16079623

Centisome position: 62.85

GC content: 44.22

Gene sequence:

>753_bases
ATGGTAACCATAAAAAAGGATTTTATACCGGTATCAAATGATAATCGTCCTGGCTATGCGATGGCGCCTGCTTATATTAC
GGTGCATAATACAGCGAATACAGCCAAAGGCGCTGATGCGAAAATGCACGCCAAGTTTGTGAAAAACCCGAACACATCCG
AAAGCTGGCATTTTACAGTTGACGATTCAGTCATTTATCAGCATCTCCCGATAGATGAAAACGGCTGGCATGCGGGAGAC
GGTACAAACGGGACAGGAAACCGCAAATCGATCGGGATTGAAATCTGTGAAAATGCTGACGGCGATTTTGAAAAAGCAAC
ATCAAATGCCCAGTGGCTGATCAGAAAGCTGATGAAGGAAAACAATATTCCGTTAAATCGTGTTGTTCCCCACAAAAAGT
GGTCCGGGAAAGAATGTCCGCGGAAACTCTTGGACCACTGGAACTCTTTTCTAAACGGAATTTCTTCCTCGGATACACCT
CCAAAAGAAACTTCACCATCATATCCTCTGCCGTCTGGGGTTATAAAGCTCACTTCGCCTTATCGAAAGGGCACAAACAT
TCTCCAGCTGCAAAAGGCTTTAGCTGTACTTCACTTTTACCCTGATAAGGGAGCCAAAAACAATGGCATAGATGGAGTTT
ACGGACCGAAAACAGCGAATGCCGTCAAGCGTTTTCAACTCATGAACGGATTAACTGCAGACGGGATTTATGGGCCAAAA
ACAAAGGCCAAGCTTAAATCGAAGTTAAAGTAA

Upstream 100 bases:

>100_bases
CAACTGAAAACCAGGTTAAGAACACAATTCTTTTTTACCTCATAAAAAAGGCTGTCCATCTCTCTGTTTTCTGCATTCTT
ATTAATAGGGAGGGATAGAA

Downstream 100 bases:

>100_bases
ATGCAGGATTTAACGGATAACCTGTTTCCAGTGCGGATTCAGGTTATTTTCTTTTCTTTGGTTTGTGTGTTTTTCAGATT
GGAAAGAAGGGAATTTTAAA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MVTIKKDFIPVSNDNRPGYAMAPAYITVHNTANTAKGADAKMHAKFVKNPNTSESWHFTVDDSVIYQHLPIDENGWHAGD
GTNGTGNRKSIGIEICENADGDFEKATSNAQWLIRKLMKENNIPLNRVVPHKKWSGKECPRKLLDHWNSFLNGISSSDTP
PKETSPSYPLPSGVIKLTSPYRKGTNILQLQKALAVLHFYPDKGAKNNGIDGVYGPKTANAVKRFQLMNGLTADGIYGPK
TKAKLKSKLK

Sequences:

>Translated_250_residues
MVTIKKDFIPVSNDNRPGYAMAPAYITVHNTANTAKGADAKMHAKFVKNPNTSESWHFTVDDSVIYQHLPIDENGWHAGD
GTNGTGNRKSIGIEICENADGDFEKATSNAQWLIRKLMKENNIPLNRVVPHKKWSGKECPRKLLDHWNSFLNGISSSDTP
PKETSPSYPLPSGVIKLTSPYRKGTNILQLQKALAVLHFYPDKGAKNNGIDGVYGPKTANAVKRFQLMNGLTADGIYGPK
TKAKLKSKLK
>Mature_250_residues
MVTIKKDFIPVSNDNRPGYAMAPAYITVHNTANTAKGADAKMHAKFVKNPNTSESWHFTVDDSVIYQHLPIDENGWHAGD
GTNGTGNRKSIGIEICENADGDFEKATSNAQWLIRKLMKENNIPLNRVVPHKKWSGKECPRKLLDHWNSFLNGISSSDTP
PKETSPSYPLPSGVIKLTSPYRKGTNILQLQKALAVLHFYPDKGAKNNGIDGVYGPKTANAVKRFQLMNGLTADGIYGPK
TKAKLKSKLK

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CWLH_BACSU (P54450)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   A69951
- RefSeq:   NP_390448.1
- ProteinModelPortal:   P54450
- EnsemblBacteria:   EBBACT00000001627
- GeneID:   937808
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25710
- NMPDR:   fig|224308.1.peg.2573
- GenoList:   BSU25710
- GeneTree:   EBGT00050000000162
- HOGENOM:   HBG712727
- OMA:   RESISVE
- ProtClustDB:   CLSK887143
- BioCyc:   BSUB:BSU25710-MONOMER
- BRENDA:   3.5.1.28
- InterPro:   IPR002502
- InterPro:   IPR002477
- Gene3D:   G3DSA:3.40.80.10
- Gene3D:   G3DSA:1.10.101.10
- SMART:   SM00644

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1; SSF55846 Amidase_2; SSF47090 PGBD_like

EC number: =3.5.1.28

Molecular weight: Translated: 27572; Mature: 27572

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTIKKDFIPVSNDNRPGYAMAPAYITVHNTANTAKGADAKMHAKFVKNPNTSESWHFTV
CEEECCCCEECCCCCCCCEEECCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCEEEEE
DDSVIYQHLPIDENGWHAGDGTNGTGNRKSIGIEICENADGDFEKATSNAQWLIRKLMKE
CCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHCCCHHHHHHHHHHH
NNIPLNRVVPHKKWSGKECPRKLLDHWNSFLNGISSSDTPPKETSPSYPLPSGVIKLTSP
CCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCC
YRKGTNILQLQKALAVLHFYPDKGAKNNGIDGVYGPKTANAVKRFQLMNGLTADGIYGPK
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
TKAKLKSKLK
HHHHHHHHCC
>Mature Secondary Structure
MVTIKKDFIPVSNDNRPGYAMAPAYITVHNTANTAKGADAKMHAKFVKNPNTSESWHFTV
CEEECCCCEECCCCCCCCEEECCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCEEEEE
DDSVIYQHLPIDENGWHAGDGTNGTGNRKSIGIEICENADGDFEKATSNAQWLIRKLMKE
CCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHCCCHHHHHHHHHHH
NNIPLNRVVPHKKWSGKECPRKLLDHWNSFLNGISSSDTPPKETSPSYPLPSGVIKLTSP
CCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCC
YRKGTNILQLQKALAVLHFYPDKGAKNNGIDGVYGPKTANAVKRFQLMNGLTADGIYGPK
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
TKAKLKSKLK
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377