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The map label for this gene is comEB

Identifier: 16079612

GI number: 16079612

Start: 2639877

End: 2640446

Strand: Reverse

Name: comEB

Synonym: BSU25580

Alternate gene names: 16079612

Gene position: 2640446-2639877 (Counterclockwise)

Preceding gene: 16079613

Following gene: 16079611

Centisome position: 62.64

GC content: 43.51

Gene sequence:

>570_bases
GTGGAACGAATTTCATGGAATCAATACTTTATGGCTCAAAGCCATTTATTGGCGCTGCGCAGCACATGTCCGAGATTATC
TGTCGGCGCTACTATAGTCAGAGACAAACGCATGATAGCAGGAGGCTACAATGGGTCAATCGCGGGCGGCGTACATTGTG
CAGATGAAGGATGCTTAATGATAGATGATCATTGCGCAAGAACCATTCACGCTGAAATGAATGCGATTCTTCAATGCTCT
AAGTTTGGGGTGCCGACTGATGGAGCCGAGATTTATGTGACGCATTATCCGTGCATTCAATGCTGCAAATCTATTATTCA
AGCTGGTATCAAAACGGTTTATTATGCTGAGGATTACAAAACACATCCGTATGCGCAGGAATTATTTGAACAGGCGGGTG
TAACTGTTGAACAGGTTGAACTCGATGAAATGATCGTTGATCTGAAAAACAGAGAAAAGCTTTCATTTGTAGCGGGTCTA
ATAGGGAAATTGGCTGATGCCGGTTTAGCGGAGGAAGAATTGAAAAAGATACATGAACAGGCGAATACACTTTTCACGAG
CTACGTGTGA

Upstream 100 bases:

>100_bases
TGAGAAAATAAAGTCTTCCATTACAGTAAAGTGATTTGACGGTCATGCATTATGATAGGTAAACTAAAGGTCAGGCAACA
CAACATTTGGAGGGAACAAC

Downstream 100 bases:

>100_bases
TGAATGCGTAATTCGCGCTTATTATTGCCTATGGCGGCAGCTTCGGCAACGGCTGGAATTACTGCCGCCGCTTATTTCCC
CGCTATTTTTCTTTTCATCC

Product: hypothetical protein

Products: dUMP; NH3

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MERISWNQYFMAQSHLLALRSTCPRLSVGATIVRDKRMIAGGYNGSIAGGVHCADEGCLMIDDHCARTIHAEMNAILQCS
KFGVPTDGAEIYVTHYPCIQCCKSIIQAGIKTVYYAEDYKTHPYAQELFEQAGVTVEQVELDEMIVDLKNREKLSFVAGL
IGKLADAGLAEEELKKIHEQANTLFTSYV

Sequences:

>Translated_189_residues
MERISWNQYFMAQSHLLALRSTCPRLSVGATIVRDKRMIAGGYNGSIAGGVHCADEGCLMIDDHCARTIHAEMNAILQCS
KFGVPTDGAEIYVTHYPCIQCCKSIIQAGIKTVYYAEDYKTHPYAQELFEQAGVTVEQVELDEMIVDLKNREKLSFVAGL
IGKLADAGLAEEELKKIHEQANTLFTSYV
>Mature_189_residues
MERISWNQYFMAQSHLLALRSTCPRLSVGATIVRDKRMIAGGYNGSIAGGVHCADEGCLMIDDHCARTIHAEMNAILQCS
KFGVPTDGAEIYVTHYPCIQCCKSIIQAGIKTVYYAEDYKTHPYAQELFEQAGVTVEQVELDEMIVDLKNREKLSFVAGL
IGKLADAGLAEEELKKIHEQANTLFTSYV

Specific function: Dispensable for transformability

COG id: COG2131

COG function: function code F; Deoxycytidylate deaminase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytidine and deoxycytidylate deaminase family

Homologues:

Organism=Homo sapiens, GI61742819, Length=160, Percent_Identity=36.25, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI61742821, Length=160, Percent_Identity=36.25, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6321938, Length=147, Percent_Identity=36.0544217687075, Blast_Score=95, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24667298, Length=150, Percent_Identity=36.6666666666667, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24667302, Length=150, Percent_Identity=36.6666666666667, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COMEB_BACSU (P32393)

Other databases:

- EMBL:   L15202
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   S39864
- RefSeq:   NP_390436.1
- ProteinModelPortal:   P32393
- SMR:   P32393
- EnsemblBacteria:   EBBACT00000001326
- GeneID:   937832
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25580
- NMPDR:   fig|224308.1.peg.2561
- GenoList:   BSU25580
- GeneTree:   EBGT00050000001533
- HOGENOM:   HBG741046
- OMA:   MERISWN
- ProtClustDB:   CLSK872992
- BioCyc:   BSUB:BSU25580-MONOMER
- InterPro:   IPR016192
- InterPro:   IPR002125
- InterPro:   IPR013404
- InterPro:   IPR015517
- InterPro:   IPR016193
- InterPro:   IPR016473
- PANTHER:   PTHR11086
- PIRSF:   PIRSF006019
- TIGRFAMs:   TIGR02571

Pfam domain/function: PF00383 dCMP_cyt_deam_1; SSF53927 Cytidine_deaminase-like

EC number: 3.5.4.12

Molecular weight: Translated: 20969; Mature: 20969

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00903 CYT_DCMP_DEAMINASES

Important sites: ACT_SITE 72-72

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERISWNQYFMAQSHLLALRSTCPRLSVGATIVRDKRMIAGGYNGSIAGGVHCADEGCLM
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCEEEECCCCCCCCCCEEECCCCCEE
IDDHCARTIHAEMNAILQCSKFGVPTDGAEIYVTHYPCIQCCKSIIQAGIKTVYYAEDYK
ECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHEEEEECCCC
THPYAQELFEQAGVTVEQVELDEMIVDLKNREKLSFVAGLIGKLADAGLAEEELKKIHEQ
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ANTLFTSYV
HHHHHHCCC
>Mature Secondary Structure
MERISWNQYFMAQSHLLALRSTCPRLSVGATIVRDKRMIAGGYNGSIAGGVHCADEGCLM
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCEEEECCCCCCCCCCEEECCCCCEE
IDDHCARTIHAEMNAILQCSKFGVPTDGAEIYVTHYPCIQCCKSIIQAGIKTVYYAEDYK
ECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHEEEEECCCC
THPYAQELFEQAGVTVEQVELDEMIVDLKNREKLSFVAGLIGKLADAGLAEEELKKIHEQ
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ANTLFTSYV
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dCMP; H2O

Specific reaction: dCMP + H2O = dUMP + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7968523; 8969508; 9384377