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The map label for this gene is comEC

Identifier: 16079611

GI number: 16079611

Start: 2637543

End: 2639873

Strand: Reverse

Name: comEC

Synonym: BSU25570

Alternate gene names: 16079611

Gene position: 2639873-2637543 (Counterclockwise)

Preceding gene: 16079612

Following gene: 16079610

Centisome position: 62.62

GC content: 44.49

Gene sequence:

>2331_bases
ATGCGTAATTCGCGCTTATTATTGCCTATGGCGGCAGCTTCGGCAACGGCTGGAATTACTGCCGCCGCTTATTTCCCCGC
TATTTTTCTTTTCATCCTCTTTCTCCTCATCATTTTAATCAAAACGAGGCACGCTTTTCTTATTATTGTTTGTTTCTTCT
CTTTTATATTGTTTTTTGTACTGTATGCAGTCACAGATTCTCAGAATGTCTCTTCCTATCGGCAGGGAACCTATCAATTC
AAGGCAGTGATTGACACTATTCCCAAAATTGACGGCGACCGTATGTCTATGATGGTTGAGACACCTGATAAGGAAAAATG
GGCTGCTGCGTATCGCATTCAGTCTGCTGGTGAAAAAGAACAGCTGTTATACATAGAACCAGGAATGTCATGTGAGTTGA
CTGGTACATTGGAAGAACCGAATCACGCAACTGTGCCGGGTGCATTTGATTATAACGAGTATCTTTATCGGCAGCATATT
CATTGGAACTACTCTGTCACGTCTATCCAAAACTGCAGCGAACCTGAAAATTTCAAGTACAAGGTGCTCAGCTTGAGAAA
ACATATCATATCATTCACAAACAGCCTTCTGCCTCCTGATTCGACAGGGATTGTACAGGCACTTACAGTTGGTGACAGAT
TTTACGTGGAGGATGAAGTGCTTACCGCGTATCAAAAGCTTGGTGTTGTCCATCTCTTGGCAATATCAGGACTCCACGTG
GGGATTTTGACAGCAGGTTTGTTTTATATCATGATCCGCCTTGGTATAACTAGAGAAAAGGCGTCAATTCTGTTGCTGTT
ATTTCTGCCGCTCTATGTGATGTTGACCGGCGCTGCTCCTTCAGTGCTACGCGCCGCTCTCATGTCGGGTGTTTACTTAG
CTGGAAGTCTTGTCAAATGGCGTGTCCGCTCTGCAACTGCAATTTGCCTTTCGTACATCGTCCTTCTGCTCTTCAATCCT
TATCATCTCTTTGAAGCCGGTTTTCAGCTATCGTTCGCCGTCAGTTTTTCTTTAATTCTATCCTCTTCTATTTTTCAGCA
GGTTAAAACCTCCTTGGGGCAGCTGACAATTGTATCACTCATCGCTCAGCTGGGCTCGCTTCCGATTCTCCTATATCATT
TTCATCAGTTTTCTATCATCAGCGTACCGATGAATATGTTGATGGTACCATTTTATACCTTCTGTATTTTGCCGGGAGCT
GTAGCAGGTGTTCTTCTATTAAGTCTTTCCGCTTCGTTTGGGAGATTGTTTTTCAGCTGGTTTGATTTATTGATAAGCTG
GATCAATAGGTTAATCACAAACATTGCAGATGTTGATGTGTTCACGATTATGATCGCACATCCTGCACCTGTTTTGCTTT
TTTTATTCACAGTCACGATCATCCTATTGCTTATGGCGATTGAAAAACGCTCCTTGTCGCAGTTGATGGTAACCGGAGGC
ATTTGCTGCACGGTGATGTTTCTGCTCTTTATATATCCGTGTCTTAGTTCCGAAGGAGAAGTGGATATGATAGATATTGG
ACAGGGTGACAGCATGTTTGTAGGTGCTCCGCATCAGCGGGGTCGTGTCTTAATTGATACCGGCGGCACTTTGTCTTACT
CGTCAGAGCCTTGGCGCGAAAAACAGCATCCGTTTTCACTGGGGGAAAAGGTGCTGATTCCGTTTTTAACTGCTAAGGGA
ATCAAGCAGCTTGACGCTTTAATTCTGACGCACGCTGACCAAGATCATATCGGAGAGGCGGAGATTCTGCTGAAGCATCA
TAAAGTAAAGCGCCTCGTGATTCCGAAAGGGTTCGTTTCTGAACCTAAAGATGAGAAAGTGCTGCAGGCAGCCAGGGAAG
AGGGAGTGGCAATTGAAGAGGTGAAGCGAGGCGATGTGTTGCAAATAAAGGATTTGCAGTTCCATGTACTGTCACCGGAA
GCACCTGATCCGGCAAGCAAAAATAATTCATCTCTCGTTCTGTGGATGGAGACGGGCGGTATGAGCTGGATCTTGACGGG
TGATCTGGAGAAAGAAGGGGAACAAGAGGTGATGAACGTGTTTCCGAATATAAAAGCAGATGTGTTAAAGGTGGGGCACC
ATGGGAGCAAAGGCTCTACCGGTGAAGAATTCATCCAACAGCTTCAGCCGAAAACGGCCATTATCTCAGCCGGGAAAAAC
AATCGGTACCATCATCCTCATCAAAAAGTTCTGCAACTATTACAGAGACATTCTATCCGCGTGCTGCGAACAGATCAAAA
CGGAACGATCCAATATAGATACAAAAACAGAGTTGGAACCTTTTCTGTCTATCCTCCATATGATACATCAGATATAACAG
AGACGAACTAA

Upstream 100 bases:

>100_bases
GGGTCTAATAGGGAAATTGGCTGATGCCGGTTTAGCGGAGGAAGAATTGAAAAAGATACATGAACAGGCGAATACACTTT
TCACGAGCTACGTGTGATGA

Downstream 100 bases:

>100_bases
AAAAGACTGCCGAGAAATCAGCAGCCCGGGCAGTCGCTGTTATGAACCAACTAGGTGAATAACCGTTGCCATGACAAACA
AGAAGGCGAAAAATCCGAAG

Product: DNA channel for uptake in competent cells

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 776; Mature: 776

Protein sequence:

>776_residues
MRNSRLLLPMAAASATAGITAAAYFPAIFLFILFLLIILIKTRHAFLIIVCFFSFILFFVLYAVTDSQNVSSYRQGTYQF
KAVIDTIPKIDGDRMSMMVETPDKEKWAAAYRIQSAGEKEQLLYIEPGMSCELTGTLEEPNHATVPGAFDYNEYLYRQHI
HWNYSVTSIQNCSEPENFKYKVLSLRKHIISFTNSLLPPDSTGIVQALTVGDRFYVEDEVLTAYQKLGVVHLLAISGLHV
GILTAGLFYIMIRLGITREKASILLLLFLPLYVMLTGAAPSVLRAALMSGVYLAGSLVKWRVRSATAICLSYIVLLLFNP
YHLFEAGFQLSFAVSFSLILSSSIFQQVKTSLGQLTIVSLIAQLGSLPILLYHFHQFSIISVPMNMLMVPFYTFCILPGA
VAGVLLLSLSASFGRLFFSWFDLLISWINRLITNIADVDVFTIMIAHPAPVLLFLFTVTIILLLMAIEKRSLSQLMVTGG
ICCTVMFLLFIYPCLSSEGEVDMIDIGQGDSMFVGAPHQRGRVLIDTGGTLSYSSEPWREKQHPFSLGEKVLIPFLTAKG
IKQLDALILTHADQDHIGEAEILLKHHKVKRLVIPKGFVSEPKDEKVLQAAREEGVAIEEVKRGDVLQIKDLQFHVLSPE
APDPASKNNSSLVLWMETGGMSWILTGDLEKEGEQEVMNVFPNIKADVLKVGHHGSKGSTGEEFIQQLQPKTAIISAGKN
NRYHHPHQKVLQLLQRHSIRVLRTDQNGTIQYRYKNRVGTFSVYPPYDTSDITETN

Sequences:

>Translated_776_residues
MRNSRLLLPMAAASATAGITAAAYFPAIFLFILFLLIILIKTRHAFLIIVCFFSFILFFVLYAVTDSQNVSSYRQGTYQF
KAVIDTIPKIDGDRMSMMVETPDKEKWAAAYRIQSAGEKEQLLYIEPGMSCELTGTLEEPNHATVPGAFDYNEYLYRQHI
HWNYSVTSIQNCSEPENFKYKVLSLRKHIISFTNSLLPPDSTGIVQALTVGDRFYVEDEVLTAYQKLGVVHLLAISGLHV
GILTAGLFYIMIRLGITREKASILLLLFLPLYVMLTGAAPSVLRAALMSGVYLAGSLVKWRVRSATAICLSYIVLLLFNP
YHLFEAGFQLSFAVSFSLILSSSIFQQVKTSLGQLTIVSLIAQLGSLPILLYHFHQFSIISVPMNMLMVPFYTFCILPGA
VAGVLLLSLSASFGRLFFSWFDLLISWINRLITNIADVDVFTIMIAHPAPVLLFLFTVTIILLLMAIEKRSLSQLMVTGG
ICCTVMFLLFIYPCLSSEGEVDMIDIGQGDSMFVGAPHQRGRVLIDTGGTLSYSSEPWREKQHPFSLGEKVLIPFLTAKG
IKQLDALILTHADQDHIGEAEILLKHHKVKRLVIPKGFVSEPKDEKVLQAAREEGVAIEEVKRGDVLQIKDLQFHVLSPE
APDPASKNNSSLVLWMETGGMSWILTGDLEKEGEQEVMNVFPNIKADVLKVGHHGSKGSTGEEFIQQLQPKTAIISAGKN
NRYHHPHQKVLQLLQRHSIRVLRTDQNGTIQYRYKNRVGTFSVYPPYDTSDITETN
>Mature_776_residues
MRNSRLLLPMAAASATAGITAAAYFPAIFLFILFLLIILIKTRHAFLIIVCFFSFILFFVLYAVTDSQNVSSYRQGTYQF
KAVIDTIPKIDGDRMSMMVETPDKEKWAAAYRIQSAGEKEQLLYIEPGMSCELTGTLEEPNHATVPGAFDYNEYLYRQHI
HWNYSVTSIQNCSEPENFKYKVLSLRKHIISFTNSLLPPDSTGIVQALTVGDRFYVEDEVLTAYQKLGVVHLLAISGLHV
GILTAGLFYIMIRLGITREKASILLLLFLPLYVMLTGAAPSVLRAALMSGVYLAGSLVKWRVRSATAICLSYIVLLLFNP
YHLFEAGFQLSFAVSFSLILSSSIFQQVKTSLGQLTIVSLIAQLGSLPILLYHFHQFSIISVPMNMLMVPFYTFCILPGA
VAGVLLLSLSASFGRLFFSWFDLLISWINRLITNIADVDVFTIMIAHPAPVLLFLFTVTIILLLMAIEKRSLSQLMVTGG
ICCTVMFLLFIYPCLSSEGEVDMIDIGQGDSMFVGAPHQRGRVLIDTGGTLSYSSEPWREKQHPFSLGEKVLIPFLTAKG
IKQLDALILTHADQDHIGEAEILLKHHKVKRLVIPKGFVSEPKDEKVLQAAREEGVAIEEVKRGDVLQIKDLQFHVLSPE
APDPASKNNSSLVLWMETGGMSWILTGDLEKEGEQEVMNVFPNIKADVLKVGHHGSKGSTGEEFIQQLQPKTAIISAGKN
NRYHHPHQKVLQLLQRHSIRVLRTDQNGTIQYRYKNRVGTFSVYPPYDTSDITETN

Specific function: The comE operon is required for the binding and uptake of transforming DNA. ComEC is required for internalization but is dispensable for DNA binding

COG id: COG0658

COG function: function code R; Predicted membrane metal-binding protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI

Homologues:

Organism=Escherichia coli, GI87081801, Length=603, Percent_Identity=26.2023217247098, Blast_Score=130, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COMEC_BACSU (P39695)

Other databases:

- EMBL:   L15202
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   S39865
- RefSeq:   NP_390435.1
- ProteinModelPortal:   P39695
- EnsemblBacteria:   EBBACT00000003877
- GeneID:   937839
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25570
- NMPDR:   fig|224308.1.peg.2560
- GenoList:   BSU25570
- GeneTree:   EBGT00050000000167
- HOGENOM:   HBG314146
- OMA:   PILLYHF
- ProtClustDB:   CLSK2391278
- BioCyc:   BSUB:BSU25570-MONOMER
- GO:   GO:0006810
- InterPro:   IPR001279
- InterPro:   IPR004477
- InterPro:   IPR004797
- SMART:   SM00849
- TIGRFAMs:   TIGR00360
- TIGRFAMs:   TIGR00361

Pfam domain/function: PF03772 Competence; PF00753 Lactamase_B

EC number: NA

Molecular weight: Translated: 86703; Mature: 86703

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xecf3bd0)-; HASH(0x110c472c)-; HASH(0x10c1a0ac)-; HASH(0x11081ec0)-; HASH(0xfabc858)-; HASH(0x110f7dd8)-; HASH(0x10dad9e0)-; HASH(0x110f57ac)-; HASH(0x110d02e8)-; HASH(0x11072450)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNSRLLLPMAAASATAGITAAAYFPAIFLFILFLLIILIKTRHAFLIIVCFFSFILFFV
CCCCCEEEEEHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LYAVTDSQNVSSYRQGTYQFKAVIDTIPKIDGDRMSMMVETPDKEKWAAAYRIQSAGEKE
HHHHCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCC
QLLYIEPGMSCELTGTLEEPNHATVPGAFDYNEYLYRQHIHWNYSVTSIQNCSEPENFKY
CEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCCCCCCCHH
KVLSLRKHIISFTNSLLPPDSTGIVQALTVGDRFYVEDEVLTAYQKLGVVHLLAISGLHV
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEHHHHHHHHHHHHHHHHHHHCCHHH
GILTAGLFYIMIRLGITREKASILLLLFLPLYVMLTGAAPSVLRAALMSGVYLAGSLVKW
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RVRSATAICLSYIVLLLFNPYHLFEAGFQLSFAVSFSLILSSSIFQQVKTSLGQLTIVSL
HHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAQLGSLPILLYHFHQFSIISVPMNMLMVPFYTFCILPGAVAGVLLLSLSASFGRLFFSW
HHHHCCCCEEEEHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDLLISWINRLITNIADVDVFTIMIAHPAPVLLFLFTVTIILLLMAIEKRSLSQLMVTGG
HHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ICCTVMFLLFIYPCLSSEGEVDMIDIGQGDSMFVGAPHQRGRVLIDTGGTLSYSSEPWRE
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCCCEEEEECCCEECCCCCCCCC
KQHPFSLGEKVLIPFLTAKGIKQLDALILTHADQDHIGEAEILLKHHKVKRLVIPKGFVS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEECCCCCCC
EPKDEKVLQAAREEGVAIEEVKRGDVLQIKDLQFHVLSPEAPDPASKNNSSLVLWMETGG
CCCHHHHHHHHHHCCCCHHHHCCCCEEEEECCEEEEECCCCCCCCCCCCCEEEEEEECCC
MSWILTGDLEKEGEQEVMNVFPNIKADVLKVGHHGSKGSTGEEFIQQLQPKTAIISAGKN
EEEEEECCCCCCHHHHHHHHCCCCCHHHHEECCCCCCCCCHHHHHHHHCCCEEEEECCCC
NRYHHPHQKVLQLLQRHSIRVLRTDQNGTIQYRYKNRVGTFSVYPPYDTSDITETN
CCCCCHHHHHHHHHHHCCEEEEEECCCCEEEEEEECCCCEEEECCCCCCCCCCCCH
>Mature Secondary Structure
MRNSRLLLPMAAASATAGITAAAYFPAIFLFILFLLIILIKTRHAFLIIVCFFSFILFFV
CCCCCEEEEEHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LYAVTDSQNVSSYRQGTYQFKAVIDTIPKIDGDRMSMMVETPDKEKWAAAYRIQSAGEKE
HHHHCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCC
QLLYIEPGMSCELTGTLEEPNHATVPGAFDYNEYLYRQHIHWNYSVTSIQNCSEPENFKY
CEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCCCCCCCHH
KVLSLRKHIISFTNSLLPPDSTGIVQALTVGDRFYVEDEVLTAYQKLGVVHLLAISGLHV
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEHHHHHHHHHHHHHHHHHHHCCHHH
GILTAGLFYIMIRLGITREKASILLLLFLPLYVMLTGAAPSVLRAALMSGVYLAGSLVKW
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RVRSATAICLSYIVLLLFNPYHLFEAGFQLSFAVSFSLILSSSIFQQVKTSLGQLTIVSL
HHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAQLGSLPILLYHFHQFSIISVPMNMLMVPFYTFCILPGAVAGVLLLSLSASFGRLFFSW
HHHHCCCCEEEEHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDLLISWINRLITNIADVDVFTIMIAHPAPVLLFLFTVTIILLLMAIEKRSLSQLMVTGG
HHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ICCTVMFLLFIYPCLSSEGEVDMIDIGQGDSMFVGAPHQRGRVLIDTGGTLSYSSEPWRE
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCCCEEEEECCCEECCCCCCCCC
KQHPFSLGEKVLIPFLTAKGIKQLDALILTHADQDHIGEAEILLKHHKVKRLVIPKGFVS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEECCCCCCC
EPKDEKVLQAAREEGVAIEEVKRGDVLQIKDLQFHVLSPEAPDPASKNNSSLVLWMETGG
CCCHHHHHHHHHHCCCCHHHHCCCCEEEEECCEEEEECCCCCCCCCCCCCEEEEEEECCC
MSWILTGDLEKEGEQEVMNVFPNIKADVLKVGHHGSKGSTGEEFIQQLQPKTAIISAGKN
EEEEEECCCCCCHHHHHHHHCCCCCHHHHEECCCCCCCCCHHHHHHHHCCCEEEEECCCC
NRYHHPHQKVLQLLQRHSIRVLRTDQNGTIQYRYKNRVGTFSVYPPYDTSDITETN
CCCCCHHHHHHHHHHHCCEEEEEECCCCEEEEEEECCCCEEEECCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968523; 8969508; 9384377