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The map label for this gene is holA

Identifier: 16079610

GI number: 16079610

Start: 2636096

End: 2637139

Strand: Reverse

Name: holA

Synonym: BSU25560

Alternate gene names: 16079610

Gene position: 2637139-2636096 (Counterclockwise)

Preceding gene: 16079611

Following gene: 16079608

Centisome position: 62.56

GC content: 41.67

Gene sequence:

>1044_bases
ATGGTATTTGATGTGTGGAAAAGCCTGAAAAAAGGAGAGGTCCATCCGGTTTATTGTTTATACGGAAAAGAGACATATCT
GCTGCAAGAAACCGTCAGCAGGATCAGACAGACGGTCGTTGATCAGGAGACAAAGGATTTCAATCTGTCCGTTTTTGATC
TGGAAGAGGACCCGCTGGATCAAGCGATTGCAGATGCTGAAACGTTTCCGTTTATGGGGGAGCGGCGTCTTGTCATTGTG
AAAAATCCATATTTTTTAACAGGTGAAAAGAAAAAAGAAAAAATTGAGCACAACGTCAGTGCGCTTGAATCATATATACA
ATCACCTGCGCCTTACACAGTCTTTGTCCTGCTTGCTCCGTATGAAAAGCTTGATGAGCGAAAAAAGCTGACGAAAGCGT
TAAAGAAGCACGCGTTTATGATGGAGGCGAAGGAATTAAATGCAAAGGAAACGACAGACTTTACTGTCAACCTTGCAAAA
ACAGAGCAGAAAACAATCGGCACGGAAGCGGCGGAGCATTTGGTTCTGCTTGTGAACGGTCATCTGTCATCGATTTTTCA
GGAGATTCAAAAGCTCTGCACGTTTATTGGAGATCGTGAAGAAATTACGCTGGATGATGTAAAAATGCTTGTTGCTAGAA
GCCTTGAACAAAATATTTTTGAGCTGATCAATAAAATCGTCAACCGAAAACGAACAGAGAGTCTGCAAATTTTTTATGAT
TTGCTAAAACAAAATGAAGAACCGATCAAAATTATGGCGCTTATTTCGAATCAGTTCCGGCTGATTCTGCAGACGAAGTA
CTTCGCGGAACAGGGATACGGACAAAAACAAATCGCTTCTAATCTAAAAGTTCACCCATTTCGGGTAAAGCTGGCGATGG
ATCAAGCAAGGCTTTTTTCAGAGGAGGAGCTCCGTTTAATTATTGAACAGCTTGCCGTCATGGACTATGAGATGAAAACC
GGGAAAAAGGACAAGCAGCTCCTGCTCGAACTGTTCCTTTTACAGCTGTTAAAAAGAAATGAAAAAAACGATCCCCATTA
TTGA

Upstream 100 bases:

>100_bases
TAATATAAATGGGGAAGCAGCAGTCAGGCTTCCTCTTTACTATTTTGCAAACTCAATTTAGGATAGAAGGGGATGATGAA
AGAGAGGAAGTCGTACAACA

Downstream 100 bases:

>100_bases
GGATCGTTTTTATATTATGCAGAAAGTCCGTTCACTTTTTTCGCTAGTCTTGATTTGTATCGAGCTGCGGTATTTTTGTG
TACAAGACCTGTTTTCACTG

Product: DNA polymerase III subunit delta

Products: diphosphate; DNAn+1

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MVFDVWKSLKKGEVHPVYCLYGKETYLLQETVSRIRQTVVDQETKDFNLSVFDLEEDPLDQAIADAETFPFMGERRLVIV
KNPYFLTGEKKKEKIEHNVSALESYIQSPAPYTVFVLLAPYEKLDERKKLTKALKKHAFMMEAKELNAKETTDFTVNLAK
TEQKTIGTEAAEHLVLLVNGHLSSIFQEIQKLCTFIGDREEITLDDVKMLVARSLEQNIFELINKIVNRKRTESLQIFYD
LLKQNEEPIKIMALISNQFRLILQTKYFAEQGYGQKQIASNLKVHPFRVKLAMDQARLFSEEELRLIIEQLAVMDYEMKT
GKKDKQLLLELFLLQLLKRNEKNDPHY

Sequences:

>Translated_347_residues
MVFDVWKSLKKGEVHPVYCLYGKETYLLQETVSRIRQTVVDQETKDFNLSVFDLEEDPLDQAIADAETFPFMGERRLVIV
KNPYFLTGEKKKEKIEHNVSALESYIQSPAPYTVFVLLAPYEKLDERKKLTKALKKHAFMMEAKELNAKETTDFTVNLAK
TEQKTIGTEAAEHLVLLVNGHLSSIFQEIQKLCTFIGDREEITLDDVKMLVARSLEQNIFELINKIVNRKRTESLQIFYD
LLKQNEEPIKIMALISNQFRLILQTKYFAEQGYGQKQIASNLKVHPFRVKLAMDQARLFSEEELRLIIEQLAVMDYEMKT
GKKDKQLLLELFLLQLLKRNEKNDPHY
>Mature_347_residues
MVFDVWKSLKKGEVHPVYCLYGKETYLLQETVSRIRQTVVDQETKDFNLSVFDLEEDPLDQAIADAETFPFMGERRLVIV
KNPYFLTGEKKKEKIEHNVSALESYIQSPAPYTVFVLLAPYEKLDERKKLTKALKKHAFMMEAKELNAKETTDFTVNLAK
TEQKTIGTEAAEHLVLLVNGHLSSIFQEIQKLCTFIGDREEITLDDVKMLVARSLEQNIFELINKIVNRKRTESLQIFYD
LLKQNEEPIKIMALISNQFRLILQTKYFAEQGYGQKQIASNLKVHPFRVKLAMDQARLFSEEELRLIIEQLAVMDYEMKT
GKKDKQLLLELFLLQLLKRNEKNDPHY

Specific function: Unknown

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YQEN_BACSU (P54459)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   B69952
- RefSeq:   NP_390434.1
- ProteinModelPortal:   P54459
- EnsemblBacteria:   EBBACT00000002330
- GeneID:   937833
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25560
- NMPDR:   fig|224308.1.peg.2559
- GenoList:   BSU25560
- GeneTree:   EBGT00050000001794
- HOGENOM:   HBG314147
- OMA:   LTYSYFD
- ProtClustDB:   PRK05574
- BioCyc:   BSUB:BSU25560-MONOMER
- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790
- TIGRFAMs:   TIGR01128

Pfam domain/function: PF06144 DNA_pol3_delta; SSF48019 Pol_clamp_load_C

EC number: 2.7.7.7

Molecular weight: Translated: 40504; Mature: 40504

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFDVWKSLKKGEVHPVYCLYGKETYLLQETVSRIRQTVVDQETKDFNLSVFDLEEDPLD
CCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHH
QAIADAETFPFMGERRLVIVKNPYFLTGEKKKEKIEHNVSALESYIQSPAPYTVFVLLAP
HHHHHHHCCCCCCCCEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
YEKLDERKKLTKALKKHAFMMEAKELNAKETTDFTVNLAKTEQKTIGTEAAEHLVLLVNG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHEEEEEECC
HLSSIFQEIQKLCTFIGDREEITLDDVKMLVARSLEQNIFELINKIVNRKRTESLQIFYD
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LLKQNEEPIKIMALISNQFRLILQTKYFAEQGYGQKQIASNLKVHPFRVKLAMDQARLFS
HHHCCCCCEEEHEEHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEEEEHHHHHHCC
EEELRLIIEQLAVMDYEMKTGKKDKQLLLELFLLQLLKRNEKNDPHY
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVFDVWKSLKKGEVHPVYCLYGKETYLLQETVSRIRQTVVDQETKDFNLSVFDLEEDPLD
CCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHH
QAIADAETFPFMGERRLVIVKNPYFLTGEKKKEKIEHNVSALESYIQSPAPYTVFVLLAP
HHHHHHHCCCCCCCCEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
YEKLDERKKLTKALKKHAFMMEAKELNAKETTDFTVNLAKTEQKTIGTEAAEHLVLLVNG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHEEEEEECC
HLSSIFQEIQKLCTFIGDREEITLDDVKMLVARSLEQNIFELINKIVNRKRTESLQIFYD
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LLKQNEEPIKIMALISNQFRLILQTKYFAEQGYGQKQIASNLKVHPFRVKLAMDQARLFS
HHHCCCCCEEEHEEHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEEEEHHHHHHCC
EEELRLIIEQLAVMDYEMKTGKKDKQLLLELFLLQLLKRNEKNDPHY
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377