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The map label for this gene is bkdAB

Identifier: 16079460

GI number: 16079460

Start: 2498093

End: 2499076

Strand: Reverse

Name: bkdAB

Synonym: BSU24040

Alternate gene names: 16079460

Gene position: 2499076-2498093 (Counterclockwise)

Preceding gene: 16079461

Following gene: 16079459

Centisome position: 59.28

GC content: 48.48

Gene sequence:

>984_bases
ATGTCAGTAATGTCATATATTGATGCAATCAATTTGGCGATGAAAGAAGAAATGGAACGAGATTCTCGCGTTTTCGTCCT
TGGGGAAGATGTAGGAAGAAAAGGCGGTGTGTTTAAAGCGACAGCGGGACTCTATGAACAATTTGGGGAAGAGCGCGTTA
TGGATACGCCGCTTGCTGAATCTGCAATCGCAGGAGTCGGTATCGGAGCGGCAATGTACGGAATGAGACCGATTGCTGAA
ATGCAGTTTGCTGATTTCATTATGCCGGCAGTCAACCAAATTATTTCTGAAGCGGCTAAAATCCGCTACCGCAGCAACAA
TGACTGGAGCTGTCCGATTGTCGTCAGAGCGCCATACGGCGGAGGCGTGCACGGAGCCCTGTATCATTCTCAATCAGTCG
AAGCAATTTTCGCCAACCAGCCCGGACTGAAAATTGTCATGCCATCAACACCATATGACGCGAAAGGGCTCTTAAAAGCC
GCAGTTCGTGACGAAGACCCCGTGCTGTTTTTTGAGCACAAGCGGGCATACCGTCTGATAAAGGGCGAGGTTCCGGCTGA
TGATTATGTCCTGCCAATCGGCAAGGCGGACGTAAAAAGGGAAGGCGACGACATCACAGTGATCACATACGGCCTGTGTG
TCCACTTCGCCTTACAAGCTGCAGAACGTCTCGAAAAAGATGGCATTTCAGCGCATGTGGTGGATTTAAGAACAGTTTAC
CCGCTTGATAAAGAAGCCATCATCGAAGCTGCGTCCAAAACTGGAAAGGTTCTTTTGGTCACAGAAGATACAAAAGAAGG
CAGCATCATGAGCGAAGTAGCCGCAATTATATCCGAGCATTGTCTGTTCGACTTAGACGCGCCGATCAAACGGCTTGCAG
GTCCTGATATTCCGGCTATGCCTTATGCGCCGACAATGGAAAAATACTTTATGGTCAACCCTGATAAAGTGGAAGCGGCG
ATGAGAGAATTAGCGGAGTTTTAA

Upstream 100 bases:

>100_bases
GCAATCGTAAATGAAGCGACGGATGAAGCGGAGAACGCCCCATATGCAGCTCCTGAGTCAGCGCTTGATTATGTTTATGC
GAAGTAGGGAGGAAGAACAA

Downstream 100 bases:

>100_bases
AGACGTAAGGGAGGATACAATCATGGCAATTGAACAAATGACGATGCCGCAGCTTGGAGAAAGCGTAACAGAGGGGACGA
TCAGCAAATGGCTTGTCGCC

Product: branched-chain alpha-keto acid dehydrogenase E1 subunit

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSVMSYIDAINLAMKEEMERDSRVFVLGEDVGRKGGVFKATAGLYEQFGEERVMDTPLAESAIAGVGIGAAMYGMRPIAE
MQFADFIMPAVNQIISEAAKIRYRSNNDWSCPIVVRAPYGGGVHGALYHSQSVEAIFANQPGLKIVMPSTPYDAKGLLKA
AVRDEDPVLFFEHKRAYRLIKGEVPADDYVLPIGKADVKREGDDITVITYGLCVHFALQAAERLEKDGISAHVVDLRTVY
PLDKEAIIEAASKTGKVLLVTEDTKEGSIMSEVAAIISEHCLFDLDAPIKRLAGPDIPAMPYAPTMEKYFMVNPDKVEAA
MRELAEF

Sequences:

>Translated_327_residues
MSVMSYIDAINLAMKEEMERDSRVFVLGEDVGRKGGVFKATAGLYEQFGEERVMDTPLAESAIAGVGIGAAMYGMRPIAE
MQFADFIMPAVNQIISEAAKIRYRSNNDWSCPIVVRAPYGGGVHGALYHSQSVEAIFANQPGLKIVMPSTPYDAKGLLKA
AVRDEDPVLFFEHKRAYRLIKGEVPADDYVLPIGKADVKREGDDITVITYGLCVHFALQAAERLEKDGISAHVVDLRTVY
PLDKEAIIEAASKTGKVLLVTEDTKEGSIMSEVAAIISEHCLFDLDAPIKRLAGPDIPAMPYAPTMEKYFMVNPDKVEAA
MRELAEF
>Mature_326_residues
SVMSYIDAINLAMKEEMERDSRVFVLGEDVGRKGGVFKATAGLYEQFGEERVMDTPLAESAIAGVGIGAAMYGMRPIAEM
QFADFIMPAVNQIISEAAKIRYRSNNDWSCPIVVRAPYGGGVHGALYHSQSVEAIFANQPGLKIVMPSTPYDAKGLLKAA
VRDEDPVLFFEHKRAYRLIKGEVPADDYVLPIGKADVKREGDDITVITYGLCVHFALQAAERLEKDGISAHVVDLRTVYP
LDKEAIIEAASKTGKVLLVTEDTKEGSIMSEVAAIISEHCLFDLDAPIKRLAGPDIPAMPYAPTMEKYFMVNPDKVEAAM
RELAEF

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=326, Percent_Identity=40.7975460122699, Blast_Score=271, Evalue=7e-73,
Organism=Homo sapiens, GI34101272, Length=326, Percent_Identity=40.7975460122699, Blast_Score=271, Evalue=7e-73,
Organism=Homo sapiens, GI156564403, Length=304, Percent_Identity=40.7894736842105, Blast_Score=238, Evalue=5e-63,
Organism=Homo sapiens, GI291084858, Length=304, Percent_Identity=39.4736842105263, Blast_Score=221, Evalue=9e-58,
Organism=Homo sapiens, GI133778974, Length=196, Percent_Identity=28.0612244897959, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=326, Percent_Identity=43.2515337423313, Blast_Score=265, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI17506935, Length=316, Percent_Identity=43.3544303797468, Blast_Score=248, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=39.4495412844037, Blast_Score=243, Evalue=2e-65,
Organism=Drosophila melanogaster, GI160714828, Length=326, Percent_Identity=42.0245398773006, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI160714832, Length=326, Percent_Identity=42.0245398773006, Blast_Score=259, Evalue=1e-69,
Organism=Drosophila melanogaster, GI21358145, Length=326, Percent_Identity=41.1042944785276, Blast_Score=251, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24650940, Length=326, Percent_Identity=41.1042944785276, Blast_Score=251, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=43.3333333333333, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=43.3333333333333, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ODBB_BACSU (P37941)

Other databases:

- EMBL:   M97391
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   D69593
- RefSeq:   NP_390284.1
- ProteinModelPortal:   P37941
- SMR:   P37941
- EnsemblBacteria:   EBBACT00000003335
- GeneID:   938672
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24040
- NMPDR:   fig|224308.1.peg.2408
- GenoList:   BSU24040
- GeneTree:   EBGT00050000000572
- HOGENOM:   HBG753264
- OMA:   LYEQFGE
- PhylomeDB:   P37941
- ProtClustDB:   CLSK873415
- BioCyc:   BSUB:BSU24040-MONOMER
- BioCyc:   MetaCyc:MONOMER-11684
- BRENDA:   1.2.4.4
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476
- Gene3D:   G3DSA:3.40.50.920
- SMART:   SM00861

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C; SSF52922 Transketo_C_like

EC number: =1.2.4.4

Molecular weight: Translated: 35857; Mature: 35726

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: ACT_SITE 129-129 BINDING 29-29 BINDING 82-82 BINDING 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVMSYIDAINLAMKEEMERDSRVFVLGEDVGRKGGVFKATAGLYEQFGEERVMDTPLAE
CCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHHCCCCCCCCCHHH
SAIAGVGIGAAMYGMRPIAEMQFADFIMPAVNQIISEAAKIRYRSNNDWSCPIVVRAPYG
HHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCC
GGVHGALYHSQSVEAIFANQPGLKIVMPSTPYDAKGLLKAAVRDEDPVLFFEHKRAYRLI
CCCCHHEECCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCHHHHHH
KGEVPADDYVLPIGKADVKREGDDITVITYGLCVHFALQAAERLEKDGISAHVVDLRTVY
CCCCCCCCEEEECCCCHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEC
PLDKEAIIEAASKTGKVLLVTEDTKEGSIMSEVAAIISEHCLFDLDAPIKRLAGPDIPAM
CCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHEECCCHHHHHHCCCCCCCC
PYAPTMEKYFMVNPDKVEAAMRELAEF
CCCCCCCCEEEECCHHHHHHHHHHHCC
>Mature Secondary Structure 
SVMSYIDAINLAMKEEMERDSRVFVLGEDVGRKGGVFKATAGLYEQFGEERVMDTPLAE
CHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHHCCCCCCCCCHHH
SAIAGVGIGAAMYGMRPIAEMQFADFIMPAVNQIISEAAKIRYRSNNDWSCPIVVRAPYG
HHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCC
GGVHGALYHSQSVEAIFANQPGLKIVMPSTPYDAKGLLKAAVRDEDPVLFFEHKRAYRLI
CCCCHHEECCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCHHHHHH
KGEVPADDYVLPIGKADVKREGDDITVITYGLCVHFALQAAERLEKDGISAHVVDLRTVY
CCCCCCCCEEEECCCCHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEC
PLDKEAIIEAASKTGKVLLVTEDTKEGSIMSEVAAIISEHCLFDLDAPIKRLAGPDIPAM
CCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHEECCCHHHHHHCCCCCCCC
PYAPTMEKYFMVNPDKVEAAMRELAEF
CCCCCCCCEEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377