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The map label for this gene is bkdB

Identifier: 16079459

GI number: 16079459

Start: 2496796

End: 2498070

Strand: Reverse

Name: bkdB

Synonym: BSU24030

Alternate gene names: 16079459

Gene position: 2498070-2496796 (Counterclockwise)

Preceding gene: 16079460

Following gene: 16079455

Centisome position: 59.26

GC content: 46.12

Gene sequence:

>1275_bases
ATGGCAATTGAACAAATGACGATGCCGCAGCTTGGAGAAAGCGTAACAGAGGGGACGATCAGCAAATGGCTTGTCGCCCC
CGGTGATAAAGTGAACAAATACGATCCGATCGCGGAAGTCATGACAGATAAGGTAAATGCAGAGGTTCCGTCTTCTTTTA
CTGGTACGATAACAGAGCTTGTGGGAGAAGAAGGCCAAACCCTGCAAGTCGGAGAAATGATTTGCAAAATTGAAACAGAA
GGCGCGAATCCGGCTGAACAAAAACAAGAACAGCCAGCAGCATCAGAAGCCGCTGAGAACCCTGTTGCAAAAAGTGCTGG
AGCAGCCGATCAGCCCAATAAAAAGCGCTACTCGCCAGCTGTTCTCCGTTTGGCCGGAGAGCACGGCATTGACCTCGATC
AAGTGACAGGAACTGGTGCCGGCGGGCGCATCACACGAAAAGATATTCAGCGCTTAATTGAAACAGGCGGCGTGCAAGAA
CAGAATCCTGAGGAGCTGAAAACAGCAGCTCCTGCACCGAAGTCTGCATCAAAACCTGAGCCAAAAGAAGAGACGTCATA
TCCTGCGTCTGCAGCCGGTGATAAAGAAATCCCTGTCACAGGTGTAAGAAAAGCAATTGCTTCCAATATGAAGCGAAGCA
AAACAGAAATTCCGCATGCTTGGACGATGATGGAAGTCGACGTCACAAATATGGTTGCATATCGCAACAGTATAAAAGAT
TCTTTTAAGAAGACAGAAGGCTTTAATTTAACGTTCTTCGCCTTTTTTGTAAAAGCGGTCGCTCAGGCGTTAAAAGAATT
CCCGCAAATGAATAGCATGTGGGCGGGGGACAAAATTATTCAGAAAAAGGATATCAATATTTCAATTGCAGTTGCCACAG
AGGATTCTTTATTTGTTCCGGTGATTAAAAACGCTGATGAAAAAACAATTAAAGGCATTGCGAAAGACATTACCGGCCTA
GCTAAAAAAGTAAGAGACGGAAAACTCACTGCAGATGACATGCAGGGAGGCACGTTTACCGTCAACAACACAGGTTCGTT
CGGGTCTGTTCAGTCGATGGGCATTATCAACTACCCTCAGGCTGCGATTCTTCAAGTAGAATCCATCGTCAAACGCCCGG
TTGTCATGGACAATGGCATGATTGCTGTCAGAGACATGGTTAATCTGTGCCTGTCATTAGATCACAGAGTGCTTGACGGT
CTCGTGTGCGGACGATTCCTCGGACGAGTGAAACAAATTTTAGAATCGATTGACGAGAAGACATCTGTTTACTAA

Upstream 100 bases:

>100_bases
GCGCCGACAATGGAAAAATACTTTATGGTCAACCCTGATAAAGTGGAAGCGGCGATGAGAGAATTAGCGGAGTTTTAAAG
ACGTAAGGGAGGATACAATC

Downstream 100 bases:

>100_bases
ATAAGCAAAAAGAGCATTTTTTGAAGTTTTGTTTCAAAAAATGCTCTTTTTCTATGCTTTATTATTCAGCGATCCGTATT
TTCATTTCGACTCGATATTC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MAIEQMTMPQLGESVTEGTISKWLVAPGDKVNKYDPIAEVMTDKVNAEVPSSFTGTITELVGEEGQTLQVGEMICKIETE
GANPAEQKQEQPAASEAAENPVAKSAGAADQPNKKRYSPAVLRLAGEHGIDLDQVTGTGAGGRITRKDIQRLIETGGVQE
QNPEELKTAAPAPKSASKPEPKEETSYPASAAGDKEIPVTGVRKAIASNMKRSKTEIPHAWTMMEVDVTNMVAYRNSIKD
SFKKTEGFNLTFFAFFVKAVAQALKEFPQMNSMWAGDKIIQKKDINISIAVATEDSLFVPVIKNADEKTIKGIAKDITGL
AKKVRDGKLTADDMQGGTFTVNNTGSFGSVQSMGIINYPQAAILQVESIVKRPVVMDNGMIAVRDMVNLCLSLDHRVLDG
LVCGRFLGRVKQILESIDEKTSVY

Sequences:

>Translated_424_residues
MAIEQMTMPQLGESVTEGTISKWLVAPGDKVNKYDPIAEVMTDKVNAEVPSSFTGTITELVGEEGQTLQVGEMICKIETE
GANPAEQKQEQPAASEAAENPVAKSAGAADQPNKKRYSPAVLRLAGEHGIDLDQVTGTGAGGRITRKDIQRLIETGGVQE
QNPEELKTAAPAPKSASKPEPKEETSYPASAAGDKEIPVTGVRKAIASNMKRSKTEIPHAWTMMEVDVTNMVAYRNSIKD
SFKKTEGFNLTFFAFFVKAVAQALKEFPQMNSMWAGDKIIQKKDINISIAVATEDSLFVPVIKNADEKTIKGIAKDITGL
AKKVRDGKLTADDMQGGTFTVNNTGSFGSVQSMGIINYPQAAILQVESIVKRPVVMDNGMIAVRDMVNLCLSLDHRVLDG
LVCGRFLGRVKQILESIDEKTSVY
>Mature_423_residues
AIEQMTMPQLGESVTEGTISKWLVAPGDKVNKYDPIAEVMTDKVNAEVPSSFTGTITELVGEEGQTLQVGEMICKIETEG
ANPAEQKQEQPAASEAAENPVAKSAGAADQPNKKRYSPAVLRLAGEHGIDLDQVTGTGAGGRITRKDIQRLIETGGVQEQ
NPEELKTAAPAPKSASKPEPKEETSYPASAAGDKEIPVTGVRKAIASNMKRSKTEIPHAWTMMEVDVTNMVAYRNSIKDS
FKKTEGFNLTFFAFFVKAVAQALKEFPQMNSMWAGDKIIQKKDINISIAVATEDSLFVPVIKNADEKTIKGIAKDITGLA
KKVRDGKLTADDMQGGTFTVNNTGSFGSVQSMGIINYPQAAILQVESIVKRPVVMDNGMIAVRDMVNLCLSLDHRVLDGL
VCGRFLGRVKQILESIDEKTSVY

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=31.2638580931264, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=31.2638580931264, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI110671329, Length=429, Percent_Identity=31.7016317016317, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI31711992, Length=441, Percent_Identity=30.3854875283447, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI19923748, Length=246, Percent_Identity=35.7723577235772, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI260898739, Length=157, Percent_Identity=38.8535031847134, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1786946, Length=415, Percent_Identity=33.2530120481928, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1786305, Length=437, Percent_Identity=32.4942791762014, Blast_Score=181, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17537937, Length=422, Percent_Identity=29.1469194312796, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI25146366, Length=410, Percent_Identity=30.7317073170732, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17560088, Length=448, Percent_Identity=29.2410714285714, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17538894, Length=306, Percent_Identity=30.0653594771242, Blast_Score=124, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=423, Percent_Identity=32.387706855792, Blast_Score=192, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6324258, Length=444, Percent_Identity=27.7027027027027, Blast_Score=146, Evalue=5e-36,
Organism=Drosophila melanogaster, GI18859875, Length=426, Percent_Identity=31.6901408450704, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI20129315, Length=223, Percent_Identity=35.4260089686099, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24582497, Length=223, Percent_Identity=35.4260089686099, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24645909, Length=223, Percent_Identity=35.4260089686099, Blast_Score=144, Evalue=9e-35,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ODB2_BACSU (P37942)

Other databases:

- EMBL:   M97391
- EMBL:   D84432
- EMBL:   AL009126
- EMBL:   L25604
- PIR:   S32488
- RefSeq:   NP_390283.1
- ProteinModelPortal:   P37942
- EnsemblBacteria:   EBBACT00000000425
- GeneID:   938677
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU24030
- NMPDR:   fig|224308.1.peg.2407
- GenoList:   BSU24030
- GeneTree:   EBGT00050000001223
- HOGENOM:   HBG630916
- OMA:   PVIMENG
- PhylomeDB:   P37942
- ProtClustDB:   PRK11856
- BioCyc:   BSUB:BSU24030-MONOMER
- BioCyc:   MetaCyc:MONOMER-11686
- BRENDA:   2.3.1.168
- GO:   GO:0006096
- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- Gene3D:   G3DSA:4.10.320.10
- Gene3D:   G3DSA:3.30.559.10

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding; SSF47005 E3_bd; SSF51230 Hybrid_motif

EC number: =2.3.1.168

Molecular weight: Translated: 45838; Mature: 45707

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50968 BIOTINYL_LIPOYL; PS00189 LIPOYL

Important sites: ACT_SITE 395-395 ACT_SITE 399-399

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEQMTMPQLGESVTEGTISKWLVAPGDKVNKYDPIAEVMTDKVNAEVPSSFTGTITEL
CCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHH
VGEEGQTLQVGEMICKIETEGANPAEQKQEQPAASEAAENPVAKSAGAADQPNKKRYSPA
HCCCCCEEEHHHEEEEEECCCCCHHHHHHCCCCHHHHHCCCCHHCCCCCCCCCCCCCCHH
VLRLAGEHGIDLDQVTGTGAGGRITRKDIQRLIETGGVQEQNPEELKTAAPAPKSASKPE
HHEECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCC
PKEETSYPASAAGDKEIPVTGVRKAIASNMKRSKTEIPHAWTMMEVDVTNMVAYRNSIKD
CCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHH
SFKKTEGFNLTFFAFFVKAVAQALKEFPQMNSMWAGDKIIQKKDINISIAVATEDSLFVP
HHHHCCCCCHHHHHHHHHHHHHHHHHCHHHCCCCCCCHHEEECCCEEEEEEECCCCEEEH
VIKNADEKTIKGIAKDITGLAKKVRDGKLTADDMQGGTFTVNNTGSFGSVQSMGIINYPQ
HHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCEEEECCCCCCCCHHCCCCCCCCH
AAILQVESIVKRPVVMDNGMIAVRDMVNLCLSLDHRVLDGLVCGRFLGRVKQILESIDEK
HHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSVY
HCCC
>Mature Secondary Structure 
AIEQMTMPQLGESVTEGTISKWLVAPGDKVNKYDPIAEVMTDKVNAEVPSSFTGTITEL
CCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHH
VGEEGQTLQVGEMICKIETEGANPAEQKQEQPAASEAAENPVAKSAGAADQPNKKRYSPA
HCCCCCEEEHHHEEEEEECCCCCHHHHHHCCCCHHHHHCCCCHHCCCCCCCCCCCCCCHH
VLRLAGEHGIDLDQVTGTGAGGRITRKDIQRLIETGGVQEQNPEELKTAAPAPKSASKPE
HHEECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCC
PKEETSYPASAAGDKEIPVTGVRKAIASNMKRSKTEIPHAWTMMEVDVTNMVAYRNSIKD
CCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHH
SFKKTEGFNLTFFAFFVKAVAQALKEFPQMNSMWAGDKIIQKKDINISIAVATEDSLFVP
HHHHCCCCCHHHHHHHHHHHHHHHHHCHHHCCCCCCCHHEEECCCEEEEEEECCCCEEEH
VIKNADEKTIKGIAKDITGLAKKVRDGKLTADDMQGGTFTVNNTGSFGSVQSMGIINYPQ
HHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCEEEECCCCCCCCHHCCCCCCCCH
AAILQVESIVKRPVVMDNGMIAVRDMVNLCLSLDHRVLDGLVCGRFLGRVKQILESIDEK
HHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSVY
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792