Click here to switch to the map view.

The map label for this gene is aroE

Identifier: 16079317

GI number: 16079317

Start: 2367954

End: 2369240

Strand: Reverse

Name: aroE

Synonym: BSU22600

Alternate gene names: 16079317

Gene position: 2369240-2367954 (Counterclockwise)

Preceding gene: 16079318

Following gene: 16079316

Centisome position: 56.2

GC content: 46.54

Gene sequence:

>1287_bases
ATGAAACGAGATAAGGTGCAGACCTTACATGGAGAAATACATATTCCCGGTGATAAATCCATTTCTCACCGCTCTGTTAT
GTTTGGCGCGCTAGCGGCAGGCACAACAACAGTTAAAAACTTTCTGCCGGGAGCAGATTGTCTGAGCACGATCGATTGCT
TTAGAAAAATGGGTGTTCACATTGAGCAAAGCAGCAGCGATGTCGTGATTCACGGAAAAGGAATCGATGCCCTGAAAGAG
CCAGAAAGCCTTTTAGATGTCGGAAATTCAGGTACAACGATTCGCCTGATGCTCGGAATATTGGCGGGCCGTCCTTTTTA
CAGCGCGGTAGCCGGAGATGAGAGCATTGCGAAACGCCCAATGAAGCGTGTGACTGAGCCTTTGAAAAAAATGGGGGCTA
AAATCGACGGCAGAGCCGGCGGAGAGTTTACACCGCTGTCAGTGAGCGGCGCTTCATTAAAAGGAATTGATTATGTATCA
CCTGTTGCAAGCGCGCAAATTAAATCTGCTGTTTTGCTGGCCGGATTACAGGCTGAGGGCACAACAACTGTAACAGAGCC
CCATAAATCTCGGGACCACACTGAGCGGATGCTTTCTGCTTTTGGCGTTAAGCTTTCTGAAGATCAAACGAGTGTTTCCA
TTGCTGGTGGCCAGAAACTGACAGCTGCTGATATTTTTGTTCCTGGAGACATTTCTTCAGCCGCGTTTTTCCTTGCTGCT
GGCGCGATGGTTCCAAACAGCAGAATTGTATTGAAAAACGTAGGTTTAAATCCGACTCGGACAGGTATTATTGATGTCCT
TCAAAACATGGGGGCAAAACTTGAAATCAAACCATCTGCTGATAGCGGTGCAGAGCCTTATGGAGATTTGATTATAGAAA
CGTCATCTCTAAAGGCAGTTGAAATCGGAGGAGATATCATTCCGCGTTTAATTGATGAGATCCCTATCATCGCGCTTCTT
GCGACTCAGGCGGAAGGAACCACCGTTATTAAGGACGCGGCAGAGCTAAAAGTGAAAGAAACAAACCGTATTGATACTGT
TGTTTCTGAGCTTCGCAAGCTGGGTGCTGAAATTGAACCGACAGCAGATGGAATGAAGGTTTATGGCAAACAAACGTTGA
AAGGCGGCGCTGCAGTGTCCAGCCACGGAGATCATCGAATCGGAATGATGCTTGGTATTGCTTCCTGTATAACGGAGGAG
CCGATTGAAATCGAGCACACGGATGCCATTCACGTTTCTTATCCAACCTTCTTCGAGCATTTAAATAAGCTTTCGAAAAA
ATCCTGA

Upstream 100 bases:

>100_bases
AGGATCAGTTTTCAGTCTGATGACGACCGCAAAAGGGCAGAACAATGCATTGAAGCCCGGGCGGAATATGAAACTTTTTA
TGCTGATTGAGGTGGAAAAA

Downstream 100 bases:

>100_bases
AGTTTTACTTCAGGATTTTTTATGCAGATCATAATTCGAGGCGAAAAAACATAGCTTGTCTTATAGAACGGATAGAAAAA
CGAAAAGGGGGGATGTGCTT

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKRDKVQTLHGEIHIPGDKSISHRSVMFGALAAGTTTVKNFLPGADCLSTIDCFRKMGVHIEQSSSDVVIHGKGIDALKE
PESLLDVGNSGTTIRLMLGILAGRPFYSAVAGDESIAKRPMKRVTEPLKKMGAKIDGRAGGEFTPLSVSGASLKGIDYVS
PVASAQIKSAVLLAGLQAEGTTTVTEPHKSRDHTERMLSAFGVKLSEDQTSVSIAGGQKLTAADIFVPGDISSAAFFLAA
GAMVPNSRIVLKNVGLNPTRTGIIDVLQNMGAKLEIKPSADSGAEPYGDLIIETSSLKAVEIGGDIIPRLIDEIPIIALL
ATQAEGTTVIKDAAELKVKETNRIDTVVSELRKLGAEIEPTADGMKVYGKQTLKGGAAVSSHGDHRIGMMLGIASCITEE
PIEIEHTDAIHVSYPTFFEHLNKLSKKS

Sequences:

>Translated_428_residues
MKRDKVQTLHGEIHIPGDKSISHRSVMFGALAAGTTTVKNFLPGADCLSTIDCFRKMGVHIEQSSSDVVIHGKGIDALKE
PESLLDVGNSGTTIRLMLGILAGRPFYSAVAGDESIAKRPMKRVTEPLKKMGAKIDGRAGGEFTPLSVSGASLKGIDYVS
PVASAQIKSAVLLAGLQAEGTTTVTEPHKSRDHTERMLSAFGVKLSEDQTSVSIAGGQKLTAADIFVPGDISSAAFFLAA
GAMVPNSRIVLKNVGLNPTRTGIIDVLQNMGAKLEIKPSADSGAEPYGDLIIETSSLKAVEIGGDIIPRLIDEIPIIALL
ATQAEGTTVIKDAAELKVKETNRIDTVVSELRKLGAEIEPTADGMKVYGKQTLKGGAAVSSHGDHRIGMMLGIASCITEE
PIEIEHTDAIHVSYPTFFEHLNKLSKKS
>Mature_428_residues
MKRDKVQTLHGEIHIPGDKSISHRSVMFGALAAGTTTVKNFLPGADCLSTIDCFRKMGVHIEQSSSDVVIHGKGIDALKE
PESLLDVGNSGTTIRLMLGILAGRPFYSAVAGDESIAKRPMKRVTEPLKKMGAKIDGRAGGEFTPLSVSGASLKGIDYVS
PVASAQIKSAVLLAGLQAEGTTTVTEPHKSRDHTERMLSAFGVKLSEDQTSVSIAGGQKLTAADIFVPGDISSAAFFLAA
GAMVPNSRIVLKNVGLNPTRTGIIDVLQNMGAKLEIKPSADSGAEPYGDLIIETSSLKAVEIGGDIIPRLIDEIPIIALL
ATQAEGTTVIKDAAELKVKETNRIDTVVSELRKLGAEIEPTADGMKVYGKQTLKGGAAVSSHGDHRIGMMLGIASCITEE
PIEIEHTDAIHVSYPTFFEHLNKLSKKS

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=438, Percent_Identity=28.0821917808219, Blast_Score=135, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6320332, Length=463, Percent_Identity=24.4060475161987, Blast_Score=104, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_BACSU (P20691)

Other databases:

- EMBL:   M80245
- EMBL:   AL009126
- PIR:   C26532
- RefSeq:   NP_390141.1
- ProteinModelPortal:   P20691
- SMR:   P20691
- EnsemblBacteria:   EBBACT00000004002
- GeneID:   939014
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22600
- NMPDR:   fig|224308.1.peg.2264
- GenoList:   BSU22600
- GeneTree:   EBGT00050000001036
- HOGENOM:   HBG646626
- OMA:   PAPSRDH
- PhylomeDB:   P20691
- ProtClustDB:   PRK02427
- BioCyc:   BSUB:BSU22600-MONOMER
- BRENDA:   2.5.1.19
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 45241; Mature: 45241

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRDKVQTLHGEIHIPGDKSISHRSVMFGALAAGTTTVKNFLPGADCLSTIDCFRKMGVH
CCCCCCEEECCEEECCCCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHCCE
IEQSSSDVVIHGKGIDALKEPESLLDVGNSGTTIRLMLGILAGRPFYSAVAGDESIAKRP
EECCCCCEEEECCCCCHHHCCHHHHHCCCCCCCHHHHHHHHCCCCHHHHHCCCHHHHHHH
MKRVTEPLKKMGAKIDGRAGGEFTPLSVSGASLKGIDYVSPVASAQIKSAVLLAGLQAEG
HHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCHHCHHHHHHHHHHHHEEECCCCC
TTTVTEPHKSRDHTERMLSAFGVKLSEDQTSVSIAGGQKLTAADIFVPGDISSAAFFLAA
CEEECCCCCCHHHHHHHHHHHCCEECCCCCEEEECCCCEEEEEEEEECCCCCHHHHHHHH
GAMVPNSRIVLKNVGLNPTRTGIIDVLQNMGAKLEIKPSADSGAEPYGDLIIETSSLKAV
CCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEECCCEEE
EIGGDIIPRLIDEIPIIALLATQAEGTTVIKDAAELKVKETNRIDTVVSELRKLGAEIEP
ECCHHHHHHHHHHCCEEEEEEECCCCCEEEECHHHEEECCCCHHHHHHHHHHHHCCCCCC
TADGMKVYGKQTLKGGAAVSSHGDHRIGMMLGIASCITEEPIEIEHTDAIHVSYPTFFEH
CCCCHHHHCHHHCCCCCEECCCCCCHHHHHHHHHHHHCCCCCEEECCCEEEEECHHHHHH
LNKLSKKS
HHHHHCCC
>Mature Secondary Structure
MKRDKVQTLHGEIHIPGDKSISHRSVMFGALAAGTTTVKNFLPGADCLSTIDCFRKMGVH
CCCCCCEEECCEEECCCCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHCCE
IEQSSSDVVIHGKGIDALKEPESLLDVGNSGTTIRLMLGILAGRPFYSAVAGDESIAKRP
EECCCCCEEEECCCCCHHHCCHHHHHCCCCCCCHHHHHHHHCCCCHHHHHCCCHHHHHHH
MKRVTEPLKKMGAKIDGRAGGEFTPLSVSGASLKGIDYVSPVASAQIKSAVLLAGLQAEG
HHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCHHCHHHHHHHHHHHHEEECCCCC
TTTVTEPHKSRDHTERMLSAFGVKLSEDQTSVSIAGGQKLTAADIFVPGDISSAAFFLAA
CEEECCCCCCHHHHHHHHHHHCCEECCCCCEEEECCCCEEEEEEEEECCCCCHHHHHHHH
GAMVPNSRIVLKNVGLNPTRTGIIDVLQNMGAKLEIKPSADSGAEPYGDLIIETSSLKAV
CCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEECCCEEE
EIGGDIIPRLIDEIPIIALLATQAEGTTVIKDAAELKVKETNRIDTVVSELRKLGAEIEP
ECCHHHHHHHHHHCCEEEEEEECCCCCEEEECHHHEEECCCCHHHHHHHHHHHHCCCCCC
TADGMKVYGKQTLKGGAAVSSHGDHRIGMMLGIASCITEEPIEIEHTDAIHVSYPTFFEH
CCCCHHHHCHHHCCCCCEECCCCCCHHHHHHHHHHHHCCCCCEEECCCEEEEECHHHHHH
LNKLSKKS
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3106153; 9384377