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The map label for this gene is birA

Identifier: 16079301

GI number: 16079301

Start: 2354918

End: 2355895

Strand: Reverse

Name: birA

Synonym: BSU22440

Alternate gene names: 16079301

Gene position: 2355895-2354918 (Counterclockwise)

Preceding gene: 16079302

Following gene: 16079300

Centisome position: 55.89

GC content: 44.79

Gene sequence:

>978_bases
ATGCGGTCAACATTAAGAAAAGACCTTATTGAATTATTTTCTCAGGCCGGCAATGAATTTATTTCCGGCCAAAAAATCAG
TGATGCTCTCGGCTGTTCAAGAACTGCTGTGTGGAAGCATATTGAAGAGCTTCGGAAAGAGGGTTATGAAGTAGAAGCCG
TTAGAAGAAAAGGATATCGGCTCATCAAAAAACCCGGAAAACTCAGTGAAAGCGAAATTCGTTTTGGATTAAAAACGGAA
GTGATGGGCCAGCATCTTATTTACCATGACGTTCTTTCAAGCACGCAAAAAACGGCTCATGAGCTCGCGAATAATAACGC
ACCGGAAGGCACCCTTGTGGTGGCTGACAAACAAACAGCCGGAAGGGGCCGAATGTCTAGGGTATGGCATTCTCAAGAAG
GAAACGGTGTTTGGATGAGCCTGATTTTGCGGCCTGACATTCCGCTCCAAAAAACACCGCAGCTGACTCTGCTTGCTGCA
GTAGCTGTTGTGCAGGGAATAGAAGAGGCAGCAGGCATCCAAACGGATATTAAATGGCCAAATGATATTTTGATTAACGG
AAAAAAAACAGTCGGTATCCTAACGGAAATGCAGGCTGAAGAAGACCGCGTACGTTCAGTGATCATTGGGATCGGCATTA
ACGTTAACCAGCAGCCTAATGATTTTCCAGATGAATTGAAGGACATCGCGACAAGCCTCAGCCAAGCTGCTGGGGAAAAA
ATTGATCGGGCCGGCGTCATCCAGCATATTTTACTATGCTTTGAGAAACGGTACCGGGATTATATGACGCACGGATTTAC
GCCGATTAAGCTTTTATGGGAAAGCTATGCGCTCGGTATTGGCACTAATATGAGAGCCAGAACGTTAAACGGAACCTTTT
ACGGTAAGGCGTTAGGTATAGATGATGAAGGCGTTCTGCTTTTAGAAACGAACGAAGGCATTAAAAAAATCTATTCTGCC
GATATCGAATTGGGCTAA

Upstream 100 bases:

>100_bases
CCGGAAAATGGGTGTCAGAAGAATTACAGTGGATCGAGCAGGCTGTTGTGACAGGGAAGCTCTCCAATCAAAAGAAACAC
ATAGAGGAGTGGCTGAAGAC

Downstream 100 bases:

>100_bases
TGTGTTGGTACAAGCCCGTTGATTTTGGTATACTTCCATTGGGCAGTATCGCCTGCGAACTGCACCTATTATTAAAATAG
ATAGACATTGCAGCAGTCTG

Product: biotin acetyl-CoA-carboxylase ligase and biotin regulon repressor

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MRSTLRKDLIELFSQAGNEFISGQKISDALGCSRTAVWKHIEELRKEGYEVEAVRRKGYRLIKKPGKLSESEIRFGLKTE
VMGQHLIYHDVLSSTQKTAHELANNNAPEGTLVVADKQTAGRGRMSRVWHSQEGNGVWMSLILRPDIPLQKTPQLTLLAA
VAVVQGIEEAAGIQTDIKWPNDILINGKKTVGILTEMQAEEDRVRSVIIGIGINVNQQPNDFPDELKDIATSLSQAAGEK
IDRAGVIQHILLCFEKRYRDYMTHGFTPIKLLWESYALGIGTNMRARTLNGTFYGKALGIDDEGVLLLETNEGIKKIYSA
DIELG

Sequences:

>Translated_325_residues
MRSTLRKDLIELFSQAGNEFISGQKISDALGCSRTAVWKHIEELRKEGYEVEAVRRKGYRLIKKPGKLSESEIRFGLKTE
VMGQHLIYHDVLSSTQKTAHELANNNAPEGTLVVADKQTAGRGRMSRVWHSQEGNGVWMSLILRPDIPLQKTPQLTLLAA
VAVVQGIEEAAGIQTDIKWPNDILINGKKTVGILTEMQAEEDRVRSVIIGIGINVNQQPNDFPDELKDIATSLSQAAGEK
IDRAGVIQHILLCFEKRYRDYMTHGFTPIKLLWESYALGIGTNMRARTLNGTFYGKALGIDDEGVLLLETNEGIKKIYSA
DIELG
>Mature_325_residues
MRSTLRKDLIELFSQAGNEFISGQKISDALGCSRTAVWKHIEELRKEGYEVEAVRRKGYRLIKKPGKLSESEIRFGLKTE
VMGQHLIYHDVLSSTQKTAHELANNNAPEGTLVVADKQTAGRGRMSRVWHSQEGNGVWMSLILRPDIPLQKTPQLTLLAA
VAVVQGIEEAAGIQTDIKWPNDILINGKKTVGILTEMQAEEDRVRSVIIGIGINVNQQPNDFPDELKDIATSLSQAAGEK
IDRAGVIQHILLCFEKRYRDYMTHGFTPIKLLWESYALGIGTNMRARTLNGTFYGKALGIDDEGVLLLETNEGIKKIYSA
DIELG

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=243, Percent_Identity=26.3374485596708, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790408, Length=322, Percent_Identity=27.0186335403727, Blast_Score=105, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24644246, Length=160, Percent_Identity=34.375, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36183; Mature: 36183

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSTLRKDLIELFSQAGNEFISGQKISDALGCSRTAVWKHIEELRKEGYEVEAVRRKGYR
CCHHHHHHHHHHHHHCCHHHCCCCHHHHHHCCCHHHHHHHHHHHHHCCCEEHHHHHCCHH
LIKKPGKLSESEIRFGLKTEVMGQHLIYHDVLSSTQKTAHELANNNAPEGTLVVADKQTA
HHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
GRGRMSRVWHSQEGNGVWMSLILRPDIPLQKTPQLTLLAAVAVVQGIEEAAGIQTDIKWP
CCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
NDILINGKKTVGILTEMQAEEDRVRSVIIGIGINVNQQPNDFPDELKDIATSLSQAAGEK
CCEEECCCCCEEHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IDRAGVIQHILLCFEKRYRDYMTHGFTPIKLLWESYALGIGTNMRARTLNGTFYGKALGI
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCEEEEEEECC
DDEGVLLLETNEGIKKIYSADIELG
CCCCEEEEECCHHHHHHHHCCCCCC
>Mature Secondary Structure
MRSTLRKDLIELFSQAGNEFISGQKISDALGCSRTAVWKHIEELRKEGYEVEAVRRKGYR
CCHHHHHHHHHHHHHCCHHHCCCCHHHHHHCCCHHHHHHHHHHHHHCCCEEHHHHHCCHH
LIKKPGKLSESEIRFGLKTEVMGQHLIYHDVLSSTQKTAHELANNNAPEGTLVVADKQTA
HHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
GRGRMSRVWHSQEGNGVWMSLILRPDIPLQKTPQLTLLAAVAVVQGIEEAAGIQTDIKWP
CCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
NDILINGKKTVGILTEMQAEEDRVRSVIIGIGINVNQQPNDFPDELKDIATSLSQAAGEK
CCEEECCCCCEEHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IDRAGVIQHILLCFEKRYRDYMTHGFTPIKLLWESYALGIGTNMRARTLNGTFYGKALGI
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCEEEEEEECC
DDEGVLLLETNEGIKKIYSADIELG
CCCCEEEEECCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]