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The map label for this gene is panB

Identifier: 16079300

GI number: 16079300

Start: 2353839

End: 2354672

Strand: Reverse

Name: panB

Synonym: BSU22430

Alternate gene names: 16079300

Gene position: 2354672-2353839 (Counterclockwise)

Preceding gene: 16079301

Following gene: 16079299

Centisome position: 55.86

GC content: 46.16

Gene sequence:

>834_bases
ATGAAAACAAAACTGGATTTTCTAAAAATGAAGGAGTCTGAAGAACCGATTGTCATGCTGACCGCTTATGATTATCCGGC
AGCTAAACTTGCTGAACAAGCGGGAGTTGACATGATTTTAGTCGGTGATTCACTTGGAATGGTCGTCCTCGGCCTTGATT
CAACTGTCGGTGTGACAGTTGCGGACATGATCCATCATACAAAAGCCGTTAAAAGGGGTGCGCCGAATACCTTTATTGTG
ACAGATATGCCGTTTATGTCTTATCACCTGTCTAAGGAAGATACGCTGAAAAATGCAGCGGCTATCGTTCAGGAAAGCGG
AGCTGACGCACTGAAGCTTGAGGGCGGAGAAGGCGTGTTTGAATCCATTCGCGCATTGACGCTTGGAGGCATTCCAGTAG
TCAGTCACTTAGGTTTGACACCGCAGTCAGTCGGCGTACTGGGCGGCTATAAAGTACAGGGCAAAGACGAACAAAGCGCC
AAAAAATTAATAGAAGACAGTATAAAATGCGAAGAAGCAGGAGCTATGATGCTTGTGCTGGAATGTGTGCCGGCAGAACT
CACAGCCAAAATTGCCGAGACGCTAAGCATACCGGTCATTGGAATCGGGGCTGGTGTGAAAGCGGACGGACAAGTTCTCG
TTTATCATGATATTATCGGCCACGGTGTTGAGAGAACACCTAAATTTGTAAAGCAATATACGCGCATTGATGAAACCATC
GAAACAGCAATCAGCGGATATGTTCAGGATGTAAGACATCGTGCTTTCCCTGAACAAAAGCATTCCTTTCAAATGAACCA
GACAGTGCTTGACGGCTTGTACGGGGGAAAATAA

Upstream 100 bases:

>100_bases
ACTTTAGAAAGTGAAGAATCCTTCTCGTTGTAACGGAAGGTTTTTTGGCTTGCAGAAGAAAACGGCAGATCATCTCCTCT
AAACATGAGGAGGAGAAAAC

Downstream 100 bases:

>100_bases
GATGAGACAGATTACTGATATTTCACAGCTGAAAGAAGCCATAAAACAATACCATTCAGAGGGCAAGTCAATCGGATTTG
TTCCGACGATGGGGTTTCTG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKTKLDFLKMKESEEPIVMLTAYDYPAAKLAEQAGVDMILVGDSLGMVVLGLDSTVGVTVADMIHHTKAVKRGAPNTFIV
TDMPFMSYHLSKEDTLKNAAAIVQESGADALKLEGGEGVFESIRALTLGGIPVVSHLGLTPQSVGVLGGYKVQGKDEQSA
KKLIEDSIKCEEAGAMMLVLECVPAELTAKIAETLSIPVIGIGAGVKADGQVLVYHDIIGHGVERTPKFVKQYTRIDETI
ETAISGYVQDVRHRAFPEQKHSFQMNQTVLDGLYGGK

Sequences:

>Translated_277_residues
MKTKLDFLKMKESEEPIVMLTAYDYPAAKLAEQAGVDMILVGDSLGMVVLGLDSTVGVTVADMIHHTKAVKRGAPNTFIV
TDMPFMSYHLSKEDTLKNAAAIVQESGADALKLEGGEGVFESIRALTLGGIPVVSHLGLTPQSVGVLGGYKVQGKDEQSA
KKLIEDSIKCEEAGAMMLVLECVPAELTAKIAETLSIPVIGIGAGVKADGQVLVYHDIIGHGVERTPKFVKQYTRIDETI
ETAISGYVQDVRHRAFPEQKHSFQMNQTVLDGLYGGK
>Mature_277_residues
MKTKLDFLKMKESEEPIVMLTAYDYPAAKLAEQAGVDMILVGDSLGMVVLGLDSTVGVTVADMIHHTKAVKRGAPNTFIV
TDMPFMSYHLSKEDTLKNAAAIVQESGADALKLEGGEGVFESIRALTLGGIPVVSHLGLTPQSVGVLGGYKVQGKDEQSA
KKLIEDSIKCEEAGAMMLVLECVPAELTAKIAETLSIPVIGIGAGVKADGQVLVYHDIIGHGVERTPKFVKQYTRIDETI
ETAISGYVQDVRHRAFPEQKHSFQMNQTVLDGLYGGK

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=256, Percent_Identity=47.65625, Blast_Score=245, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6319653, Length=264, Percent_Identity=37.5, Blast_Score=182, Evalue=7e-47,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_BACSU (P52996)

Other databases:

- EMBL:   L47709
- EMBL:   AL009126
- PIR:   G69671
- RefSeq:   NP_390124.1
- ProteinModelPortal:   P52996
- SMR:   P52996
- EnsemblBacteria:   EBBACT00000003524
- GeneID:   939032
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22430
- NMPDR:   fig|224308.1.peg.2247
- GenoList:   BSU22430
- GeneTree:   EBGT00050000000505
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- PhylomeDB:   P52996
- ProtClustDB:   PRK00311
- BioCyc:   BSUB:BSU22430-MONOMER
- BRENDA:   2.1.2.11
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29759; Mature: 29759

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 82-82 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKLDFLKMKESEEPIVMLTAYDYPAAKLAEQAGVDMILVGDSLGMVVLGLDSTVGVTV
CCCCCHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCCEEEECCCCCEEEEECCCCCCHHH
ADMIHHTKAVKRGAPNTFIVTDMPFMSYHLSKEDTLKNAAAIVQESGADALKLEGGEGVF
HHHHHHHHHHHCCCCCEEEEECCCHHHEECCCHHHHHHHHHHHHCCCCCEEEEECCCCHH
ESIRALTLGGIPVVSHLGLTPQSVGVLGGYKVQGKDEQSAKKLIEDSIKCEEAGAMMLVL
HHHHHHHCCCCHHHHHCCCCHHHHCCCCCEEECCCCHHHHHHHHHHHCCHHHCCCEEEEE
ECVPAELTAKIAETLSIPVIGIGAGVKADGQVLVYHDIIGHGVERTPKFVKQYTRIDETI
ECCCHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEHHHCCCHHCCHHHHHHHHHHHHHH
ETAISGYVQDVRHRAFPEQKHSFQMNQTVLDGLYGGK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTKLDFLKMKESEEPIVMLTAYDYPAAKLAEQAGVDMILVGDSLGMVVLGLDSTVGVTV
CCCCCHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCCEEEECCCCCEEEEECCCCCCHHH
ADMIHHTKAVKRGAPNTFIVTDMPFMSYHLSKEDTLKNAAAIVQESGADALKLEGGEGVF
HHHHHHHHHHHCCCCCEEEEECCCHHHEECCCHHHHHHHHHHHHCCCCCEEEEECCCCHH
ESIRALTLGGIPVVSHLGLTPQSVGVLGGYKVQGKDEQSAKKLIEDSIKCEEAGAMMLVL
HHHHHHHCCCCHHHHHCCCCHHHHCCCCCEEECCCCHHHHHHHHHHHCCHHHCCCEEEEE
ECVPAELTAKIAETLSIPVIGIGAGVKADGQVLVYHDIIGHGVERTPKFVKQYTRIDETI
ECCCHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEHHHCCCHHCCHHHHHHHHHHHHHH
ETAISGYVQDVRHRAFPEQKHSFQMNQTVLDGLYGGK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377