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The map label for this gene is cca

Identifier: 16079302

GI number: 16079302

Start: 2355880

End: 2357073

Strand: Reverse

Name: cca

Synonym: BSU22450

Alternate gene names: 16079302

Gene position: 2357073-2355880 (Counterclockwise)

Preceding gene: 16079303

Following gene: 16079301

Centisome position: 55.91

GC content: 45.98

Gene sequence:

>1194_bases
ATGGAAAAAGTTTTTATCAAAGCACTTCCCGTGCTCCGCATCTTAATCGAAGCGGGCCATCAGGCTTATTTTGTCGGGGG
CGCAGTTCGTGACAGCTATATGAAACGAACGATCGGAGATGTCGATATCGCGACAGATGCGGCACCTGATCAAGTAGAAC
GGCTGTTTCAGCGGACTGTTGATGTAGGCAAAGAGCACGGGACCATTATTGTGCTCTGGGAGGATGAAACCTATGAAGTC
ACGACATTCCGGACTGAATCTGATTATGTTGATTTCAGAAGACCGTCAGAAGTGCAATTTATCTCATCATTAGAAGAGGA
TCTAAAACGCAGAGATTTAACGATCAATGCGATGGCCATGACAGCGGACGGGAAGGTGCTTGATTATTTTGGCGGCAAGA
AAGATATTGATCAGAAAGTGATTCGAACCGTTGGAAAGCCTGAGGATAGATTTCAAGAGGATGCGCTTCGTATGCTGAGA
GCCGTACGCTTTATGAGCCAGCTTGGCTTTACGCTTTCACCGGAAACAGAAGAAGCCATTGCGAAAGAAAAATCACTCCT
CTCCCATGTTTCAGTCGAACGAAAAACTATAGAGTTTGAGAAATTGCTGCAGGGAAGAGCTTCTCGCCAGGCGCTTCAAA
CACTCATTCAAACGAGATTGTATGAAGAGCTTCCTGGTTTTTATCATAAACGAGAAAACCTGATTTCAACGAGTGAGTTT
CCATTCTTCTCTTTAACATCCCGTGAGGAGCTTTGGGCTGCTCTTTTAATTAACCTTGGCATAGTTTTGAAAGACGCGCC
CCTTTTCCTGAAAGCCTGGAAGCTCCCGGGAAAAGTGATCAAGGAAGCCATTCACATTGCCGATACGTTTGGCCAGAGCC
TTGACGCAATGACAATGTACAGAGCTGGGAAAAAAGCACTGCTCTCAGCGGCCAAAATATCGCAGCTTCGGCAAAACGAA
AAACTGGATGAAAAGAAACTGAAGGACATTCAGTACGCTTATCAAAACCTGCCGATCAAAAGCCTTAAGGATCTTGATAT
CACGGGTAAGGACTTGCTTGCGCTTCGAAACCGGCCGGCCGGAAAATGGGTGTCAGAAGAATTACAGTGGATCGAGCAGG
CTGTTGTGACAGGGAAGCTCTCCAATCAAAAGAAACACATAGAGGAGTGGCTGAAGACATGCGGTCAACATTAA

Upstream 100 bases:

>100_bases
AAGGCGGCAATAGAGATGCTTGAAAATGAATTTTCTTCTAAAAAAATTGTCAGCCAGTATGAACAGATTTATGCCGATTT
AGCAGAACCGGAGTGATCAC

Downstream 100 bases:

>100_bases
GAAAAGACCTTATTGAATTATTTTCTCAGGCCGGCAATGAATTTATTTCCGGCCAAAAAATCAGTGATGCTCTCGGCTGT
TCAAGAACTGCTGTGTGGAA

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MEKVFIKALPVLRILIEAGHQAYFVGGAVRDSYMKRTIGDVDIATDAAPDQVERLFQRTVDVGKEHGTIIVLWEDETYEV
TTFRTESDYVDFRRPSEVQFISSLEEDLKRRDLTINAMAMTADGKVLDYFGGKKDIDQKVIRTVGKPEDRFQEDALRMLR
AVRFMSQLGFTLSPETEEAIAKEKSLLSHVSVERKTIEFEKLLQGRASRQALQTLIQTRLYEELPGFYHKRENLISTSEF
PFFSLTSREELWAALLINLGIVLKDAPLFLKAWKLPGKVIKEAIHIADTFGQSLDAMTMYRAGKKALLSAAKISQLRQNE
KLDEKKLKDIQYAYQNLPIKSLKDLDITGKDLLALRNRPAGKWVSEELQWIEQAVVTGKLSNQKKHIEEWLKTCGQH

Sequences:

>Translated_397_residues
MEKVFIKALPVLRILIEAGHQAYFVGGAVRDSYMKRTIGDVDIATDAAPDQVERLFQRTVDVGKEHGTIIVLWEDETYEV
TTFRTESDYVDFRRPSEVQFISSLEEDLKRRDLTINAMAMTADGKVLDYFGGKKDIDQKVIRTVGKPEDRFQEDALRMLR
AVRFMSQLGFTLSPETEEAIAKEKSLLSHVSVERKTIEFEKLLQGRASRQALQTLIQTRLYEELPGFYHKRENLISTSEF
PFFSLTSREELWAALLINLGIVLKDAPLFLKAWKLPGKVIKEAIHIADTFGQSLDAMTMYRAGKKALLSAAKISQLRQNE
KLDEKKLKDIQYAYQNLPIKSLKDLDITGKDLLALRNRPAGKWVSEELQWIEQAVVTGKLSNQKKHIEEWLKTCGQH
>Mature_397_residues
MEKVFIKALPVLRILIEAGHQAYFVGGAVRDSYMKRTIGDVDIATDAAPDQVERLFQRTVDVGKEHGTIIVLWEDETYEV
TTFRTESDYVDFRRPSEVQFISSLEEDLKRRDLTINAMAMTADGKVLDYFGGKKDIDQKVIRTVGKPEDRFQEDALRMLR
AVRFMSQLGFTLSPETEEAIAKEKSLLSHVSVERKTIEFEKLLQGRASRQALQTLIQTRLYEELPGFYHKRENLISTSEF
PFFSLTSREELWAALLINLGIVLKDAPLFLKAWKLPGKVIKEAIHIADTFGQSLDAMTMYRAGKKALLSAAKISQLRQNE
KLDEKKLKDIQYAYQNLPIKSLKDLDITGKDLLALRNRPAGKWVSEELQWIEQAVVTGKLSNQKKHIEEWLKTCGQH

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=214, Percent_Identity=37.3831775700935, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI87081691, Length=229, Percent_Identity=38.8646288209607, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789436, Length=201, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=223, Percent_Identity=35.8744394618834, Blast_Score=121, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6321016, Length=298, Percent_Identity=28.8590604026846, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281360112, Length=210, Percent_Identity=35.2380952380952, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21357337, Length=210, Percent_Identity=35.2380952380952, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_BACSU (P42977)

Other databases:

- EMBL:   L38424
- EMBL:   L47709
- EMBL:   AL009126
- PIR:   B69672
- RefSeq:   NP_390126.1
- ProteinModelPortal:   P42977
- SMR:   P42977
- EnsemblBacteria:   EBBACT00000003170
- GeneID:   939025
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22450
- NMPDR:   fig|224308.1.peg.2249
- GenoList:   BSU22450
- GeneTree:   EBGT00050000000996
- HOGENOM:   HBG635063
- OMA:   GKASERF
- PhylomeDB:   P42977
- ProtClustDB:   PRK13299
- BioCyc:   BSUB:BSU22450-MONOMER
- BRENDA:   2.7.7.21
- BRENDA:   2.7.7.25
- HAMAP:   MF_01263
- InterPro:   IPR023068
- InterPro:   IPR002646

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 45453; Mature: 45453

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: BINDING 27-27 BINDING 30-30 BINDING 111-111 BINDING 154-154 BINDING 157-157 BINDING 160-160 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVFIKALPVLRILIEAGHQAYFVGGAVRDSYMKRTIGDVDIATDAAPDQVERLFQRTV
CCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
DVGKEHGTIIVLWEDETYEVTTFRTESDYVDFRRPSEVQFISSLEEDLKRRDLTINAMAM
HCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEE
TADGKVLDYFGGKKDIDQKVIRTVGKPEDRFQEDALRMLRAVRFMSQLGFTLSPETEEAI
ECCCCEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
AKEKSLLSHVSVERKTIEFEKLLQGRASRQALQTLIQTRLYEELPGFYHKRENLISTSEF
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCC
PFFSLTSREELWAALLINLGIVLKDAPLFLKAWKLPGKVIKEAIHIADTFGQSLDAMTMY
CEEECCCHHHHHHHHHHHHHHHEECCCHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHH
RAGKKALLSAAKISQLRQNEKLDEKKLKDIQYAYQNLPIKSLKDLDITGKDLLALRNRPA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHCCCCC
GKWVSEELQWIEQAVVTGKLSNQKKHIEEWLKTCGQH
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKVFIKALPVLRILIEAGHQAYFVGGAVRDSYMKRTIGDVDIATDAAPDQVERLFQRTV
CCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
DVGKEHGTIIVLWEDETYEVTTFRTESDYVDFRRPSEVQFISSLEEDLKRRDLTINAMAM
HCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEE
TADGKVLDYFGGKKDIDQKVIRTVGKPEDRFQEDALRMLRAVRFMSQLGFTLSPETEEAI
ECCCCEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
AKEKSLLSHVSVERKTIEFEKLLQGRASRQALQTLIQTRLYEELPGFYHKRENLISTSEF
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCC
PFFSLTSREELWAALLINLGIVLKDAPLFLKAWKLPGKVIKEAIHIADTFGQSLDAMTMY
CEEECCCHHHHHHHHHHHHHHHEECCCHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHH
RAGKKALLSAAKISQLRQNEKLDEKKLKDIQYAYQNLPIKSLKDLDITGKDLLALRNRPA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHCCCCC
GKWVSEELQWIEQAVVTGKLSNQKKHIEEWLKTCGQH
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377; 9829937