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The map label for this gene is yloN

Identifier: 16078638

GI number: 16078638

Start: 1649286

End: 1650377

Strand: Direct

Name: yloN

Synonym: BSU15750

Alternate gene names: 16078638

Gene position: 1649286-1650377 (Clockwise)

Preceding gene: 16078637

Following gene: 16078639

Centisome position: 39.12

GC content: 44.51

Gene sequence:

>1092_bases
ATGGCAGAACTTAATAAAACAAAAGTAAGAAAAGAATTGCGGACAGAGCGTCCGTCTATTTATTCTTTTGAATTAGATGA
AATCAAACAATGGCTGACAGACAATGGAGAGAAACCGTTCCGTGCAGCCCAGATCTTTGAATGGCTATATGAAAAACGCG
TATCTTCTTTTGAAGATATGACAAACCTCTCAAAGGATCTGCGTGAGAAATTAAACACTCGCTTTGTGTTAACAACGCTG
AAAACGGCTGTTAAACAAACCTCTCAAGACGGTACGATGAAGTTTTTGTTTGAGCTTCATGACGGTTATACCATCGAAAC
CGTTTTAATGAGACACGAGTATGGCAATTCTGTATGTGTAACGACACAAGTCGGCTGCCGTATTGGCTGCACATTCTGCG
CGTCAACGCTTGGAGGCTTAAAACGAAACCTTGAAGCAGGGGAAATTGTCGCTCAAGTAGTCAAAGTGCAAAAAGCTCTT
GATGAAACGGATGAACGCGTCAGCTCTGTCGTGATCATGGGAATCGGCGAACCTTTTGATAACTTTAACGAAATGCTCGC
TTTCTTGAAAATCATTAACCATGACAAGGGCCTGAATATCGGTGCTCGCCATATTACGGTCTCTACGAGTGGAATCATCC
CGAAAATTTACGAATTTGCTGATCAGCAAATGCAGATTAACTTTGCAATTTCTCTGCACGCGCCGAACACAGAAATCAGA
AGCCGTTTGATGCCGATTAACAGAGCATATAAACTGCCTGATCTAATGGAAGCCGTTAAATATTATATTAATAAAACAGG
ACGCCGTATCAGCTTTGAATACGGGCTGTTCGGAGGCGTAAACGACCAGGTGGAACACGCCGAAGAGCTTGCCGACTTGC
TGGAAGGAGTCAAATGCCACGTGAACTTGATTCCGGTAAACTACGTGCCTGAACGGGACTATGTGCGCACACCGCGTGAT
CAGATTTTTGCTTTTGAAAAAACGCTGAAATCCCGCGGAGTAAATGTCACAATCCGAAGAGAGCAAGGACATGACATTGA
CGCAGCCTGCGGTCAGCTTCGCGCGAAGGAGCGTCAAGACGAGACGAGGTGA

Upstream 100 bases:

>100_bases
GGTGAGACCCTTTGTTCGGGATGGAAGGCTTCAAATCCTTCCTCATTATTTCGGAACAGATGGTTTCTTTATTTGCAGCA
TGAGAAAGAAGGGATAAGCA

Downstream 100 bases:

>100_bases
TGAGGGTTGTTAACAGCCTTAAAAACAGATACAGGAAAAATCCGCCAGCATAATGAAGATGATGCGGGGATATTCAAGGG
GAAAGATGAATTTATATTAG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MAELNKTKVRKELRTERPSIYSFELDEIKQWLTDNGEKPFRAAQIFEWLYEKRVSSFEDMTNLSKDLREKLNTRFVLTTL
KTAVKQTSQDGTMKFLFELHDGYTIETVLMRHEYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVKVQKAL
DETDERVSSVVIMGIGEPFDNFNEMLAFLKIINHDKGLNIGARHITVSTSGIIPKIYEFADQQMQINFAISLHAPNTEIR
SRLMPINRAYKLPDLMEAVKYYINKTGRRISFEYGLFGGVNDQVEHAEELADLLEGVKCHVNLIPVNYVPERDYVRTPRD
QIFAFEKTLKSRGVNVTIRREQGHDIDAACGQLRAKERQDETR

Sequences:

>Translated_363_residues
MAELNKTKVRKELRTERPSIYSFELDEIKQWLTDNGEKPFRAAQIFEWLYEKRVSSFEDMTNLSKDLREKLNTRFVLTTL
KTAVKQTSQDGTMKFLFELHDGYTIETVLMRHEYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVKVQKAL
DETDERVSSVVIMGIGEPFDNFNEMLAFLKIINHDKGLNIGARHITVSTSGIIPKIYEFADQQMQINFAISLHAPNTEIR
SRLMPINRAYKLPDLMEAVKYYINKTGRRISFEYGLFGGVNDQVEHAEELADLLEGVKCHVNLIPVNYVPERDYVRTPRD
QIFAFEKTLKSRGVNVTIRREQGHDIDAACGQLRAKERQDETR
>Mature_362_residues
AELNKTKVRKELRTERPSIYSFELDEIKQWLTDNGEKPFRAAQIFEWLYEKRVSSFEDMTNLSKDLREKLNTRFVLTTLK
TAVKQTSQDGTMKFLFELHDGYTIETVLMRHEYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVKVQKALD
ETDERVSSVVIMGIGEPFDNFNEMLAFLKIINHDKGLNIGARHITVSTSGIIPKIYEFADQQMQINFAISLHAPNTEIRS
RLMPINRAYKLPDLMEAVKYYINKTGRRISFEYGLFGGVNDQVEHAEELADLLEGVKCHVNLIPVNYVPERDYVRTPRDQ
IFAFEKTLKSRGVNVTIRREQGHDIDAACGQLRAKERQDETR

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=334, Percent_Identity=38.3233532934132, Blast_Score=249, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41570; Mature: 41439

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELNKTKVRKELRTERPSIYSFELDEIKQWLTDNGEKPFRAAQIFEWLYEKRVSSFEDM
CCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
TNLSKDLREKLNTRFVLTTLKTAVKQTSQDGTMKFLFELHDGYTIETVLMRHEYGNSVCV
HHHHHHHHHHHCCEEHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEHHHHHHHCCCEEEE
TTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVKVQKALDETDERVSSVVIMGIGEPFD
EEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCC
NFNEMLAFLKIINHDKGLNIGARHITVSTSGIIPKIYEFADQQMQINFAISLHAPNTEIR
CHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHCCCEEEEEEEEEEECCCHHHH
SRLMPINRAYKLPDLMEAVKYYINKTGRRISFEYGLFGGVNDQVEHAEELADLLEGVKCH
HHHCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEE
VNLIPVNYVPERDYVRTPRDQIFAFEKTLKSRGVNVTIRREQGHDIDAACGQLRAKERQD
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCC
ETR
CCC
>Mature Secondary Structure 
AELNKTKVRKELRTERPSIYSFELDEIKQWLTDNGEKPFRAAQIFEWLYEKRVSSFEDM
CCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
TNLSKDLREKLNTRFVLTTLKTAVKQTSQDGTMKFLFELHDGYTIETVLMRHEYGNSVCV
HHHHHHHHHHHCCEEHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEHHHHHHHCCCEEEE
TTQVGCRIGCTFCASTLGGLKRNLEAGEIVAQVVKVQKALDETDERVSSVVIMGIGEPFD
EEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCC
NFNEMLAFLKIINHDKGLNIGARHITVSTSGIIPKIYEFADQQMQINFAISLHAPNTEIR
CHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHCCCEEEEEEEEEEECCCHHHH
SRLMPINRAYKLPDLMEAVKYYINKTGRRISFEYGLFGGVNDQVEHAEELADLLEGVKCH
HHHCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEE
VNLIPVNYVPERDYVRTPRDQIFAFEKTLKSRGVNVTIRREQGHDIDAACGQLRAKERQD
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCC
ETR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA