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The map label for this gene is sumT

Identifier: 16078625

GI number: 16078625

Start: 1634061

End: 1634834

Strand: Direct

Name: sumT

Synonym: BSU15610

Alternate gene names: 16078625

Gene position: 1634061-1634834 (Clockwise)

Preceding gene: 16078624

Following gene: 16078626

Centisome position: 38.76

GC content: 42.51

Gene sequence:

>774_bases
ATGGGGAAAGTATATATTGTAGGAGCGGGTCCCGGTGACCCTGATCTGCTGACAATTAAAGCGCTGAAAGCCATTGAAAA
AGCTGATGTCATCTTATATGACAGATTAGTAAATAAAGAGATTTTACAATATGCCAAAGAGCAAGCAGACCTGATCTACT
GCGGGAAGCTTCCGGATTTTCATACGATGAAGCAGGAAACCATCAATCGATTTCTTGTGAAGTATGCGCAAAAAGGGAAA
ATGGTTGTCAGACTGAAAGGCGGAGACCCTTTTGTGTTCGGGAGAGGAGGGGAAGAAGCGGAATGCCTCTCTGAAAATGG
AATTCCGTTTGAAATCATTCCCGGCATTACGTCTGGAATCGCGGCAGCCGCTTATGCCGGGATTCCTGTTACCCATCGGG
ATGCGGGCTCTAACGTCGCTTTTGTAACGGGTCACTATAAAAAAGAAGAAGATTTTGAGGAAAAGTGGAAAGCGCTGGCT
ACAGGAATTGATACACTTGTCATCTATATGGGTATCAAAAACGTGCAGCAGATTGAAAGAAAACTCCTTGAAAATGGCCG
GGACGGTTCTACGCCAGCAGCCTTTATTCATTGGGGGACAACGGACAAGCAAAAATCTGTTTTTTGTACAGTTGACACCC
TTTCAGAAACGGTGATAAAAGAAAACATTACAAATCCGAGTTTAATTGTCATTGGAAATGTTGTAAATTATCACTATAAG
CTGGAATGGTTTGAATCTGAACTAAAAAAACAAGATTTAAGCGAGGCGTTGTAA

Upstream 100 bases:

>100_bases
GAATATGATCTGCTGCGTTCTTCTCAGATGCCGGAATGCAAGCGAGAACATCATCACAGCGAATCTTCTGGAAGAACTCG
AATAGATCGGGGGAAATACG

Downstream 100 bases:

>100_bases
AAATGAAACAAGCAATTTTATATGTCGGCCACGGCAGCCGGGTCAAAAAAGCGCAGCAAGAAGCCGCAGCCTTTTTGGAA
GGATGCAAGGCCCACATCTC

Product: uroporphyrinogen III and precorrin-1 C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MGKVYIVGAGPGDPDLLTIKALKAIEKADVILYDRLVNKEILQYAKEQADLIYCGKLPDFHTMKQETINRFLVKYAQKGK
MVVRLKGGDPFVFGRGGEEAECLSENGIPFEIIPGITSGIAAAAYAGIPVTHRDAGSNVAFVTGHYKKEEDFEEKWKALA
TGIDTLVIYMGIKNVQQIERKLLENGRDGSTPAAFIHWGTTDKQKSVFCTVDTLSETVIKENITNPSLIVIGNVVNYHYK
LEWFESELKKQDLSEAL

Sequences:

>Translated_257_residues
MGKVYIVGAGPGDPDLLTIKALKAIEKADVILYDRLVNKEILQYAKEQADLIYCGKLPDFHTMKQETINRFLVKYAQKGK
MVVRLKGGDPFVFGRGGEEAECLSENGIPFEIIPGITSGIAAAAYAGIPVTHRDAGSNVAFVTGHYKKEEDFEEKWKALA
TGIDTLVIYMGIKNVQQIERKLLENGRDGSTPAAFIHWGTTDKQKSVFCTVDTLSETVIKENITNPSLIVIGNVVNYHYK
LEWFESELKKQDLSEAL
>Mature_256_residues
GKVYIVGAGPGDPDLLTIKALKAIEKADVILYDRLVNKEILQYAKEQADLIYCGKLPDFHTMKQETINRFLVKYAQKGKM
VVRLKGGDPFVFGRGGEEAECLSENGIPFEIIPGITSGIAAAAYAGIPVTHRDAGSNVAFVTGHYKKEEDFEEKWKALAT
GIDTLVIYMGIKNVQQIERKLLENGRDGSTPAAFIHWGTTDKQKSVFCTVDTLSETVIKENITNPSLIVIGNVVNYHYKL
EWFESELKKQDLSEAL

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789768, Length=245, Percent_Identity=46.9387755102041, Blast_Score=239, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6322922, Length=241, Percent_Identity=32.7800829875519, Blast_Score=125, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SUMT_BACSU (O34744)

Other databases:

- EMBL:   AJ000974
- EMBL:   AL009126
- PIR:   D69877
- RefSeq:   NP_389444.1
- HSSP:   P21631
- ProteinModelPortal:   O34744
- SMR:   O34744
- EnsemblBacteria:   EBBACT00000002545
- GeneID:   936712
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15610
- NMPDR:   fig|224308.1.peg.1563
- GenoList:   BSU15610
- GeneTree:   EBGT00050000002415
- HOGENOM:   HBG730212
- OMA:   TGHEAVG
- PhylomeDB:   O34744
- ProtClustDB:   CLSK887284
- BioCyc:   BSUB:BSU15610-MONOMER
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- TIGRFAMs:   TIGR01469

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.107

Molecular weight: Translated: 28516; Mature: 28385

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00839 SUMT_1; PS00840 SUMT_2

Important sites: BINDING 11-11 BINDING 117-117 BINDING 170-170

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVYIVGAGPGDPDLLTIKALKAIEKADVILYDRLVNKEILQYAKEQADLIYCGKLPDF
CCEEEEEECCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCH
HTMKQETINRFLVKYAQKGKMVVRLKGGDPFVFGRGGEEAECLSENGIPFEIIPGITSGI
HHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCHHHHHHCCCCCEEEECCCHHHH
AAAAYAGIPVTHRDAGSNVAFVTGHYKKEEDFEEKWKALATGIDTLVIYMGIKNVQQIER
HHHHHCCCCEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLLENGRDGSTPAAFIHWGTTDKQKSVFCTVDTLSETVIKENITNPSLIVIGNVVNYHYK
HHHHCCCCCCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHCCCCCCEEEEEEEEEEEEE
LEWFESELKKQDLSEAL
EEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GKVYIVGAGPGDPDLLTIKALKAIEKADVILYDRLVNKEILQYAKEQADLIYCGKLPDF
CEEEEEECCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCH
HTMKQETINRFLVKYAQKGKMVVRLKGGDPFVFGRGGEEAECLSENGIPFEIIPGITSGI
HHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCHHHHHHCCCCCEEEECCCHHHH
AAAAYAGIPVTHRDAGSNVAFVTGHYKKEEDFEEKWKALATGIDTLVIYMGIKNVQQIER
HHHHHCCCCEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLLENGRDGSTPAAFIHWGTTDKQKSVFCTVDTLSETVIKENITNPSLIVIGNVVNYHYK
HHHHCCCCCCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHCCCCCCEEEEEEEEEEEEE
LEWFESELKKQDLSEAL
EEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377