Click here to switch to the map view.

The map label for this gene is queE

Identifier: 16078438

GI number: 16078438

Start: 1440542

End: 1441273

Strand: Direct

Name: queE

Synonym: BSU13740

Alternate gene names: 16078438

Gene position: 1440542-1441273 (Clockwise)

Preceding gene: 16078437

Following gene: 16078439

Centisome position: 34.17

GC content: 46.31

Gene sequence:

>732_bases
ATGGCTAAAGGAATTCCTGTATTAGAAATTTTCGGCCCGACGATTCAAGGAGAAGGCATGGTGATCGGACAGAAAACGAT
GTTCGTCCGGACAGCCGGCTGCGATTATTCCTGCAGCTGGTGTGACTCCGCTTTCACTTGGGACGGGTCTGCTAAAAAAG
ATATTCGCTGGATGACGGCGGAGGAGATTTTTGCGGAGCTTAAGGACATTGGGGGAGATGCGTTTTCTCATGTGACGATC
TCAGGCGGCAACCCGGCTCTGTTAAAACAGCTGGATGCTTTTATTGAACTGCTGAAGGAAAATAACATCCGTGCAGCGCT
TGAAACTCAAGGGACCGTTTATCAGGATTGGTTTACGCTGATTGATGACCTGACAATTTCTCCAAAACCGCCAAGCTCAA
AAATGGTAACGAACTTTCAAAAGCTTGACCACATTTTAACTTCACTTCAAGAGAATGATAGACAGCATGCTGTCAGCTTG
AAGGTTGTCATCTTTAATGATGAAGATCTGGAATTTGCCAAAACTGTGCACAAGCGGTATCCGGGAATCCCGTTTTATTT
ACAAGTCGGAAATGATGACGTGCATACGACAGATGATCAGTCACTGATTGCACATTTGCTTGGAAAATATGAAGCGCTTG
TTGATAAGGTAGCTGTCGATGCGGAGCTGAATCTCGTCAGAGTGCTCCCGCAGCTGCACACCCTGTTATGGGGCAACAAA
CGCGGAGTATAA

Upstream 100 bases:

>100_bases
TATTTAGACACTTTGGAGAATAAGCCGACCTGTGTACAGGTGTTTGTCCGTGAAACACCGACAAGCTACTGTGTCTACCG
TCCGAAAAAGGGTGGTTTGA

Downstream 100 bases:

>100_bases
TAGAAAGGAAGATGAACATGACGACAAGAAAAGAATCAGAATTAGAAGGTGTAACATTGCTAGGCAATCAAGGTACAAAT
TATTTGTTCGAATATGCACC

Product: queuosine biosynthesis enzyme

Products: NA

Alternate protein names: CDG synthase; Queuosine biosynthesis protein queE

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MAKGIPVLEIFGPTIQGEGMVIGQKTMFVRTAGCDYSCSWCDSAFTWDGSAKKDIRWMTAEEIFAELKDIGGDAFSHVTI
SGGNPALLKQLDAFIELLKENNIRAALETQGTVYQDWFTLIDDLTISPKPPSSKMVTNFQKLDHILTSLQENDRQHAVSL
KVVIFNDEDLEFAKTVHKRYPGIPFYLQVGNDDVHTTDDQSLIAHLLGKYEALVDKVAVDAELNLVRVLPQLHTLLWGNK
RGV

Sequences:

>Translated_243_residues
MAKGIPVLEIFGPTIQGEGMVIGQKTMFVRTAGCDYSCSWCDSAFTWDGSAKKDIRWMTAEEIFAELKDIGGDAFSHVTI
SGGNPALLKQLDAFIELLKENNIRAALETQGTVYQDWFTLIDDLTISPKPPSSKMVTNFQKLDHILTSLQENDRQHAVSL
KVVIFNDEDLEFAKTVHKRYPGIPFYLQVGNDDVHTTDDQSLIAHLLGKYEALVDKVAVDAELNLVRVLPQLHTLLWGNK
RGV
>Mature_242_residues
AKGIPVLEIFGPTIQGEGMVIGQKTMFVRTAGCDYSCSWCDSAFTWDGSAKKDIRWMTAEEIFAELKDIGGDAFSHVTIS
GGNPALLKQLDAFIELLKENNIRAALETQGTVYQDWFTLIDDLTISPKPPSSKMVTNFQKLDHILTSLQENDRQHAVSLK
VVIFNDEDLEFAKTVHKRYPGIPFYLQVGNDDVHTTDDQSLIAHLLGKYEALVDKVAVDAELNLVRVLPQLHTLLWGNKR
GV

Specific function: Catalyzes the conversion of 6-carboxy-5,6,7,8- tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG)

COG id: COG0602

COG function: function code O; Organic radical activating enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. 7-carboxy-7- deazaguanine synthase family

Homologues:

Organism=Escherichia coli, GI1789139, Length=139, Percent_Identity=31.6546762589928, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEE_BACSU (O31677)

Other databases:

- EMBL:   AL009126
- PIR:   C69868
- RefSeq:   NP_389257.1
- ProteinModelPortal:   O31677
- EnsemblBacteria:   EBBACT00000002360
- GeneID:   939290
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13740
- NMPDR:   fig|224308.1.peg.1376
- GenoList:   BSU13740
- GeneTree:   EBGT00050000002810
- HOGENOM:   HBG507714
- OMA:   KPPSSGM
- PhylomeDB:   O31677
- ProtClustDB:   CLSK2391268
- BioCyc:   BSUB:BSU13740-MONOMER
- InterPro:   IPR017742
- InterPro:   IPR007197
- TIGRFAMs:   TIGR03365

Pfam domain/function: PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 27122; Mature: 26991

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGIPVLEIFGPTIQGEGMVIGQKTMFVRTAGCDYSCSWCDSAFTWDGSAKKDIRWMTA
CCCCCCEEEEECCCCCCCCEEEECEEEEEEECCCCCCCCCCCCCEECCCCCCCCEEEHHH
EEIFAELKDIGGDAFSHVTISGGNPALLKQLDAFIELLKENNIRAALETQGTVYQDWFTL
HHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
IDDLTISPKPPSSKMVTNFQKLDHILTSLQENDRQHAVSLKVVIFNDEDLEFAKTVHKRY
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHC
PGIPFYLQVGNDDVHTTDDQSLIAHLLGKYEALVDKVAVDAELNLVRVLPQLHTLLWGNK
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
RGV
CCC
>Mature Secondary Structure 
AKGIPVLEIFGPTIQGEGMVIGQKTMFVRTAGCDYSCSWCDSAFTWDGSAKKDIRWMTA
CCCCCEEEEECCCCCCCCEEEECEEEEEEECCCCCCCCCCCCCEECCCCCCCCEEEHHH
EEIFAELKDIGGDAFSHVTISGGNPALLKQLDAFIELLKENNIRAALETQGTVYQDWFTL
HHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
IDDLTISPKPPSSKMVTNFQKLDHILTSLQENDRQHAVSLKVVIFNDEDLEFAKTVHKRY
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHC
PGIPFYLQVGNDDVHTTDDQSLIAHLLGKYEALVDKVAVDAELNLVRVLPQLHTLLWGNK
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
RGV
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377