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The map label for this gene is queD

Identifier: 16078437

GI number: 16078437

Start: 1440100

End: 1440549

Strand: Direct

Name: queD

Synonym: BSU13730

Alternate gene names: 16078437

Gene position: 1440100-1440549 (Clockwise)

Preceding gene: 16078436

Following gene: 16078438

Centisome position: 34.16

GC content: 45.56

Gene sequence:

>450_bases
GTGCATAAATTGTTATCTCAAATTTATCCGCAGGCCCAGCATCCTTATTCGTTTGAACTGAATAAGGACATGCATATCTC
AGCCGCTCATTTTATCCCTCGGGAAAGTGCGGGAGCGTGCAGCAGGGTTCACGGGCATACGTATACCGTAAATATAACCG
TTGCCGGCGACGAACTTGACGATTCAGGCTTTCTTGTCAATTTCAGCGTACTCAAAAAACTGGTGCACGGAAACTATGAT
CACACGCTATTAAATGATCATGAAGATTTTTCTCAGGACGACCGGTATTCGCTGCCGACAACTGAAGTCGTGGCGAAAAC
GATTTATGACAACGTACAGGCTTATTTAGACACTTTGGAGAATAAGCCGACCTGTGTACAGGTGTTTGTCCGTGAAACAC
CGACAAGCTACTGTGTCTACCGTCCGAAAAAGGGTGGTTTGAATGGCTAA

Upstream 100 bases:

>100_bases
ACATGCTATAACGGCATCATCGCAGACGGCTGCGGCGAATGTCCGGCATGTCACCTTCGTTCAAAAGGTTATGAAGAATA
TATGGTGATGAAAGGAGAGC

Downstream 100 bases:

>100_bases
AGGAATTCCTGTATTAGAAATTTTCGGCCCGACGATTCAAGGAGAAGGCATGGTGATCGGACAGAAAACGATGTTCGTCC
GGACAGCCGGCTGCGATTAT

Product: 6-pyruvoyl tetrahydrobiopterin synthase-like; queuosine biosynthesis

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: CPH4 synthase; Queuosine biosynthesis protein queD [H]

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MHKLLSQIYPQAQHPYSFELNKDMHISAAHFIPRESAGACSRVHGHTYTVNITVAGDELDDSGFLVNFSVLKKLVHGNYD
HTLLNDHEDFSQDDRYSLPTTEVVAKTIYDNVQAYLDTLENKPTCVQVFVRETPTSYCVYRPKKGGLNG

Sequences:

>Translated_149_residues
MHKLLSQIYPQAQHPYSFELNKDMHISAAHFIPRESAGACSRVHGHTYTVNITVAGDELDDSGFLVNFSVLKKLVHGNYD
HTLLNDHEDFSQDDRYSLPTTEVVAKTIYDNVQAYLDTLENKPTCVQVFVRETPTSYCVYRPKKGGLNG
>Mature_149_residues
MHKLLSQIYPQAQHPYSFELNKDMHISAAHFIPRESAGACSRVHGHTYTVNITVAGDELDDSGFLVNFSVLKKLVHGNYD
HTLLNDHEDFSQDDRYSLPTTEVVAKTIYDNVQAYLDTLENKPTCVQVFVRETPTSYCVYRPKKGGLNG

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde [H]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789124, Length=129, Percent_Identity=34.1085271317829, Blast_Score=70, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007116
- InterPro:   IPR007115 [H]

Pfam domain/function: PF01242 PTPS [H]

EC number: 4.2.3.12

Molecular weight: Translated: 16847; Mature: 16847

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKLLSQIYPQAQHPYSFELNKDMHISAAHFIPRESAGACSRVHGHTYTVNITVAGDELD
CHHHHHHHCCCCCCCEEEECCCCCEEEHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCC
DSGFLVNFSVLKKLVHGNYDHTLLNDHEDFSQDDRYSLPTTEVVAKTIYDNVQAYLDTLE
CCCEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
NKPTCVQVFVRETPTSYCVYRPKKGGLNG
CCCCEEEEEEECCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MHKLLSQIYPQAQHPYSFELNKDMHISAAHFIPRESAGACSRVHGHTYTVNITVAGDELD
CHHHHHHHCCCCCCCEEEECCCCCEEEHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCC
DSGFLVNFSVLKKLVHGNYDHTLLNDHEDFSQDDRYSLPTTEVVAKTIYDNVQAYLDTLE
CCCEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
NKPTCVQVFVRETPTSYCVYRPKKGGLNG
CCCCEEEEEEECCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]