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The map label for this gene is mtnB

Identifier: 16078425

GI number: 16078425

Start: 1428940

End: 1429569

Strand: Direct

Name: mtnB

Synonym: BSU13610

Alternate gene names: 16078425

Gene position: 1428940-1429569 (Clockwise)

Preceding gene: 16078424

Following gene: 16078426

Centisome position: 33.9

GC content: 44.29

Gene sequence:

>630_bases
ATGGCTGCAAAACAAGAACGCTGGCGAGAGCTCGCTGAAGTAAAACGGGAATTGGCAGAGAGAGATTGGTTTCCGGCGAC
TAGCGGCAATCTGTCAATTAAGGTTACAGATGAGCCTTTAACATTTCTCGTCACTGCAAGCGGAAAAGATAAACGCAAAG
AGACGGTTGAAGATTTTCTGCTGGTCGATCAAAACGGAGAGCCTGCTGAAAGCGGGCATTCTCTGAAACCGTCAGCAGAA
ACGCTTTTGCATACACATTTGTACAATAAAACCAATGCCGGATGCTGCCTGCATGTCCATACGGTAAACAATAATGTGAT
TTCAGAGTTATACGGAGACCAAAAGAAAATCACATTCAAAGGCCAGGAAATTATAAAAGCACTCGGCTTGTGGGAAGAGA
ACGCAGAAGTGACCGTTCCGATTATAGAAAATCCGGCACATATCCCGACGCTAGCGGCACTGTTTGCGGAAGAGATTTCA
GAAGATTCAGGAGCTGTTCTGATCCGAAATCATGGTATTACGGCTTGGGGGAAAACAGCATTTGAGGCAAAAAGAGTGCT
TGAAGCGTATGAATTTTTGTTCAGCTACCATTTGAAATTGAAAACACTCGAGCATCAGCTGGTTAAATAA

Upstream 100 bases:

>100_bases
ATTTATTAAACGAATGCCGGGAGCAAAACCTCAATCATCTTCCGTATCAAGATTTTTATGAGATCAGAAAGGAAATTGAG
AATGTAAAGGAGGTACAGGA

Downstream 100 bases:

>100_bases
AAGGAGGAATTGAAATGGCTACAATTCGAATTCATGATGAGGCAAATACAACGATTGAAAATCAAGAGGAAGTGGCAAGC
TTTCTGGACAGTCAAGAGGT

Product: methylthioribulose-1-phosphate dehydratase

Products: NA

Alternate protein names: MTRu-1-P dehydratase

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MAAKQERWRELAEVKRELAERDWFPATSGNLSIKVTDEPLTFLVTASGKDKRKETVEDFLLVDQNGEPAESGHSLKPSAE
TLLHTHLYNKTNAGCCLHVHTVNNNVISELYGDQKKITFKGQEIIKALGLWEENAEVTVPIIENPAHIPTLAALFAEEIS
EDSGAVLIRNHGITAWGKTAFEAKRVLEAYEFLFSYHLKLKTLEHQLVK

Sequences:

>Translated_209_residues
MAAKQERWRELAEVKRELAERDWFPATSGNLSIKVTDEPLTFLVTASGKDKRKETVEDFLLVDQNGEPAESGHSLKPSAE
TLLHTHLYNKTNAGCCLHVHTVNNNVISELYGDQKKITFKGQEIIKALGLWEENAEVTVPIIENPAHIPTLAALFAEEIS
EDSGAVLIRNHGITAWGKTAFEAKRVLEAYEFLFSYHLKLKTLEHQLVK
>Mature_208_residues
AAKQERWRELAEVKRELAERDWFPATSGNLSIKVTDEPLTFLVTASGKDKRKETVEDFLLVDQNGEPAESGHSLKPSAET
LLHTHLYNKTNAGCCLHVHTVNNNVISELYGDQKKITFKGQEIIKALGLWEENAEVTVPIIENPAHIPTLAALFAEEISE
DSGAVLIRNHGITAWGKTAFEAKRVLEAYEFLFSYHLKLKTLEHQLVK

Specific function: Catalyzes the dehydration of methylthioribulose-1- phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1- phosphate (DK-MTP-1-P)

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. MtnB subfamily

Homologues:

Organism=Homo sapiens, GI166235186, Length=206, Percent_Identity=32.0388349514563, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6322484, Length=212, Percent_Identity=31.6037735849057, Blast_Score=83, Evalue=3e-17,
Organism=Drosophila melanogaster, GI18859685, Length=200, Percent_Identity=31, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTNB_BACSU (O31668)

Other databases:

- EMBL:   AL009126
- PIR:   A69864
- RefSeq:   NP_389244.1
- ProteinModelPortal:   O31668
- SMR:   O31668
- EnsemblBacteria:   EBBACT00000000069
- GeneID:   939320
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13610
- NMPDR:   fig|224308.1.peg.1363
- GenoList:   BSU13610
- GeneTree:   EBGT00050000002065
- HOGENOM:   HBG337716
- OMA:   EFLFECE
- PhylomeDB:   O31668
- ProtClustDB:   PRK06754
- BioCyc:   BSUB:BSU13610-MONOMER
- BioCyc:   MetaCyc:MONOMER-13279
- HAMAP:   MF_01677
- InterPro:   IPR001303
- InterPro:   IPR017714
- Gene3D:   G3DSA:3.40.225.10
- TIGRFAMs:   TIGR03328

Pfam domain/function: PF00596 Aldolase_II; SSF53639 Aldolase_II_N

EC number: =4.2.1.109

Molecular weight: Translated: 23489; Mature: 23358

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKQERWRELAEVKRELAERDWFPATSGNLSIKVTDEPLTFLVTASGKDKRKETVEDFL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHEE
LVDQNGEPAESGHSLKPSAETLLHTHLYNKTNAGCCLHVHTVNNNVISELYGDQKKITFK
EECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHCCCEEEEEC
GQEIIKALGLWEENAEVTVPIIENPAHIPTLAALFAEEISEDSGAVLIRNHGITAWGKTA
HHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHH
FEAKRVLEAYEFLFSYHLKLKTLEHQLVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AAKQERWRELAEVKRELAERDWFPATSGNLSIKVTDEPLTFLVTASGKDKRKETVEDFL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHEE
LVDQNGEPAESGHSLKPSAETLLHTHLYNKTNAGCCLHVHTVNNNVISELYGDQKKITFK
EECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHCCCEEEEEC
GQEIIKALGLWEENAEVTVPIIENPAHIPTLAALFAEEISEDSGAVLIRNHGITAWGKTA
HHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHH
FEAKRVLEAYEFLFSYHLKLKTLEHQLVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 12022921; 14551435