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The map label for this gene is mtnD

Identifier: 16078426

GI number: 16078426

Start: 1429584

End: 1430120

Strand: Direct

Name: mtnD

Synonym: BSU13620

Alternate gene names: 16078426

Gene position: 1429584-1430120 (Clockwise)

Preceding gene: 16078425

Following gene: 16078428

Centisome position: 33.91

GC content: 41.53

Gene sequence:

>537_bases
ATGGCTACAATTCGAATTCATGATGAGGCAAATACAACGATTGAAAATCAAGAGGAAGTGGCAAGCTTTCTGGACAGTCA
AGAGGTTATTTATGAGCAATGGGATATCACGAGGCTTCCGGAACATCTCTCAGAGAAATATGACCTGACAGAAGAAGAAA
AACAGCAAATTCTTGATACCTTCGAAACTGAAATTAAAGATATCTCAACCCGCCGCGGCTATAAAGCACAGGATGTTATT
TCATTGTCAGACAGCAATCCGAAACTTGATGAACTGCTTGAAAACTTTAAAAGAGAGCATCACCATACAGACGATGAGGT
TCGTTTTATTGTAAGCGGACACGGTATTTTTGTCATTCAAGGCCAGGACGGCACGTTTTTTGATGTCCGTTTAAACCCTG
GAGATTTAATCTCCGTGCCTGAGAATATCCGCCATTACTTCACGCTTCAGGAGGACCGCAAAGTTGTCGCGGTGAGAATT
TTTGTCACAACTGAAGGCTGGGTTCCTATTTATGAAAAAGACAGCGTGAATCAATAA

Upstream 100 bases:

>100_bases
AGGCAAAAAGAGTGCTTGAAGCGTATGAATTTTTGTTCAGCTACCATTTGAAATTGAAAACACTCGAGCATCAGCTGGTT
AAATAAAAGGAGGAATTGAA

Downstream 100 bases:

>100_bases
GCGTGAGATAGCCCCGTTCATCGAACGGGGCTTTTTATATTTATTGCAAACGCCACTTTTCATATTGTGACAGTTCTTTT
TTAATCAGTGTCCAAATCAA

Product: acireductone dioxygenase

Products: NA

Alternate protein names: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase; DHK-MTPene dioxygenase; Acireductone dioxygenase (Fe(2+)-requiring); ARD'; Fe-ARD; Acireductone dioxygenase (Ni(2+)-requiring); ARD; Ni-ARD

Number of amino acids: Translated: 178; Mature: 177

Protein sequence:

>178_residues
MATIRIHDEANTTIENQEEVASFLDSQEVIYEQWDITRLPEHLSEKYDLTEEEKQQILDTFETEIKDISTRRGYKAQDVI
SLSDSNPKLDELLENFKREHHHTDDEVRFIVSGHGIFVIQGQDGTFFDVRLNPGDLISVPENIRHYFTLQEDRKVVAVRI
FVTTEGWVPIYEKDSVNQ

Sequences:

>Translated_178_residues
MATIRIHDEANTTIENQEEVASFLDSQEVIYEQWDITRLPEHLSEKYDLTEEEKQQILDTFETEIKDISTRRGYKAQDVI
SLSDSNPKLDELLENFKREHHHTDDEVRFIVSGHGIFVIQGQDGTFFDVRLNPGDLISVPENIRHYFTLQEDRKVVAVRI
FVTTEGWVPIYEKDSVNQ
>Mature_177_residues
ATIRIHDEANTTIENQEEVASFLDSQEVIYEQWDITRLPEHLSEKYDLTEEEKQQILDTFETEIKDISTRRGYKAQDVIS
LSDSNPKLDELLENFKREHHHTDDEVRFIVSGHGIFVIQGQDGTFFDVRLNPGDLISVPENIRHYFTLQEDRKVVAVRIF
VTTEGWVPIYEKDSVNQ

Specific function: Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4- me

COG id: COG1791

COG function: function code S; Uncharacterized conserved protein, contains double-stranded beta-helix domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acireductone dioxygenase (ARD) family

Homologues:

Organism=Homo sapiens, GI171460928, Length=107, Percent_Identity=31.7757009345794, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323651, Length=142, Percent_Identity=28.169014084507, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI161082987, Length=106, Percent_Identity=33.0188679245283, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTND_BACSU (O31669)

Other databases:

- EMBL:   AL009126
- PIR:   B69864
- RefSeq:   NP_389245.1
- ProteinModelPortal:   O31669
- SMR:   O31669
- EnsemblBacteria:   EBBACT00000001053
- GeneID:   939322
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13620
- NMPDR:   fig|224308.1.peg.1364
- GenoList:   BSU13620
- GeneTree:   EBGT00050000002186
- HOGENOM:   HBG660685
- OMA:   AGWEAIY
- PhylomeDB:   O31669
- ProtClustDB:   CLSK887193
- BioCyc:   BSUB:BSU13620-MONOMER
- HAMAP:   MF_01682
- InterPro:   IPR004313
- InterPro:   IPR011051
- InterPro:   IPR014710
- Gene3D:   G3DSA:2.60.120.10
- PANTHER:   PTHR23418

Pfam domain/function: PF03079 ARD; SSF51182 RmlC_like_cupin

EC number: =1.13.11.54; =1.13.11.53

Molecular weight: Translated: 20824; Mature: 20693

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIRIHDEANTTIENQEEVASFLDSQEVIYEQWDITRLPEHLSEKYDLTEEEKQQILDT
CEEEEEECCCCCCCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHH
FETEIKDISTRRGYKAQDVISLSDSNPKLDELLENFKREHHHTDDEVRFIVSGHGIFVIQ
HHHHHHHHHHCCCCCCHHEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEE
GQDGTFFDVRLNPGDLISVPENIRHYFTLQEDRKVVAVRIFVTTEGWVPIYEKDSVNQ
CCCCCEEEEEECCCCEECCCCHHHEEEEECCCCEEEEEEEEEEECCCEEEECCCCCCC
>Mature Secondary Structure 
ATIRIHDEANTTIENQEEVASFLDSQEVIYEQWDITRLPEHLSEKYDLTEEEKQQILDT
EEEEEECCCCCCCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHH
FETEIKDISTRRGYKAQDVISLSDSNPKLDELLENFKREHHHTDDEVRFIVSGHGIFVIQ
HHHHHHHHHHCCCCCCHHEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEE
GQDGTFFDVRLNPGDLISVPENIRHYFTLQEDRKVVAVRIFVTTEGWVPIYEKDSVNQ
CCCCCEEEEEECCCCEECCCCHHHEEEEECCCCEEEEEEEEEEECCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 12022921; 14551435