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The map label for this gene is ogt

Identifier: 16078418

GI number: 16078418

Start: 1421426

End: 1421923

Strand: Direct

Name: ogt

Synonym: BSU13540

Alternate gene names: 16078418

Gene position: 1421426-1421923 (Clockwise)

Preceding gene: 16078417

Following gene: 16078422

Centisome position: 33.72

GC content: 48.59

Gene sequence:

>498_bases
ATGAACTACTATACGACAGCCGAAACGCCGCTCGGTGAACTTATCATTGCCGAAGAGGAGGACCGGATCACTCGTCTATT
TCTCAGTCAGGAAGATTGGGTGGATTGGAAAGAAACGGTTCAGAATACTGAGCATAAGGAAACACCTAATCTTGCAGAAG
CGAAACAACAGCTTCAAGAATATTTCGCAGGCGAAAGGAAGACATTCTCCCTGCCGCTCAGCCAAAAGGGCACTCCTTTT
CAGCAAAAAGTGTGGCAAGCGCTGGAGAGGATTCCATATGGCGAATCCCGAAGCTATGCGGATATTGCCGCTGCTGTCGG
CAGTCCGAAAGCGGTGCGCGCTGTCGGGCAGGCTAATAAACGTAACGACCTGCCGATTTTTGTCCCGTGCCACAGAGTGA
TCGGCAAAAACAGTGCGTTAACAGGATACGCCGGAAGCAAAACGGAGATCAAAGCATTTTTGCTAAACATCGAGCGAATC
TCCTATAAAGAAAAATAA

Upstream 100 bases:

>100_bases
ATCAAGGCGAGATTATGGTAGAAAGTGAAGAAGGGAAGGGCACCGTTTTCCATATTACGCTTCCTGTCAGACAGAATGCT
GAAGAAAGAAGGAATGATGA

Downstream 100 bases:

>100_bases
AACATATGGCACGTTCCCTTTTTTCACGGCCGACATATCATTTTATTAAGTTAAAAAAGTTGGTCTGATGAAAAAGGAGG
ACAAAGCAATTGAAGCCGAG

Product: O6-alkylguanine DNA alkyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MNYYTTAETPLGELIIAEEEDRITRLFLSQEDWVDWKETVQNTEHKETPNLAEAKQQLQEYFAGERKTFSLPLSQKGTPF
QQKVWQALERIPYGESRSYADIAAAVGSPKAVRAVGQANKRNDLPIFVPCHRVIGKNSALTGYAGSKTEIKAFLLNIERI
SYKEK

Sequences:

>Translated_165_residues
MNYYTTAETPLGELIIAEEEDRITRLFLSQEDWVDWKETVQNTEHKETPNLAEAKQQLQEYFAGERKTFSLPLSQKGTPF
QQKVWQALERIPYGESRSYADIAAAVGSPKAVRAVGQANKRNDLPIFVPCHRVIGKNSALTGYAGSKTEIKAFLLNIERI
SYKEK
>Mature_165_residues
MNYYTTAETPLGELIIAEEEDRITRLFLSQEDWVDWKETVQNTEHKETPNLAEAKQQLQEYFAGERKTFSLPLSQKGTPF
QQKVWQALERIPYGESRSYADIAAAVGSPKAVRAVGQANKRNDLPIFVPCHRVIGKNSALTGYAGSKTEIKAFLLNIERI
SYKEK

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family

Homologues:

Organism=Homo sapiens, GI197304670, Length=166, Percent_Identity=31.9277108433735, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1787596, Length=162, Percent_Identity=38.8888888888889, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788542, Length=144, Percent_Identity=40.9722222222222, Blast_Score=103, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI115533068, Length=77, Percent_Identity=50.6493506493506, Blast_Score=83, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=77, Percent_Identity=50.6493506493506, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=46.9879518072289, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OGT_BACSU (P11742)

Other databases:

- EMBL:   X15659
- EMBL:   AL009126
- PIR:   S04877
- RefSeq:   NP_389237.1
- ProteinModelPortal:   P11742
- SMR:   P11742
- EnsemblBacteria:   EBBACT00000002812
- GeneID:   939332
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13540
- NMPDR:   fig|224308.1.peg.1356
- GenoList:   BSU13540
- GeneTree:   EBGT00050000000104
- HOGENOM:   HBG752398
- OMA:   ENNHITH
- PhylomeDB:   P11742
- ProtClustDB:   CLSK887191
- BioCyc:   BSUB:BSU13540-MONOMER
- BRENDA:   2.1.1.63
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR10815
- TIGRFAMs:   TIGR00589

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N; SSF46767 MethylDNA_cys_mtrans_DNA_bd

EC number: =2.1.1.63

Molecular weight: Translated: 18752; Mature: 18752

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00374 MGMT

Important sites: ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYYTTAETPLGELIIAEEEDRITRLFLSQEDWVDWKETVQNTEHKETPNLAEAKQQLQE
CCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHH
YFAGERKTFSLPLSQKGTPFQQKVWQALERIPYGESRSYADIAAAVGSPKAVRAVGQANK
HHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHCCCC
RNDLPIFVPCHRVIGKNSALTGYAGSKTEIKAFLLNIERISYKEK
CCCCCEEEECHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNYYTTAETPLGELIIAEEEDRITRLFLSQEDWVDWKETVQNTEHKETPNLAEAKQQLQE
CCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHH
YFAGERKTFSLPLSQKGTPFQQKVWQALERIPYGESRSYADIAAAVGSPKAVRAVGQANK
HHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHCCCC
RNDLPIFVPCHRVIGKNSALTGYAGSKTEIKAFLLNIERISYKEK
CCCCCEEEECHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2505068; 2506524; 9384377