Click here to switch to the map view.

The map label for this gene is ykrV

Identifier: 16078422

GI number: 16078422

Start: 1425641

End: 1426837

Strand: Direct

Name: ykrV

Synonym: BSU13580

Alternate gene names: 16078422

Gene position: 1425641-1426837 (Clockwise)

Preceding gene: 16078418

Following gene: 16078423

Centisome position: 33.82

GC content: 47.03

Gene sequence:

>1197_bases
ATGAAATTTGAACAGTCTCATGTATTGAAGGAATTGCCTAAACAATTCTTCGCTTCTCTGGTGCAGAAAGTGAACCGAAA
GCTTGCAGAAGGACATGACGTTATCAATCTGGGACAGGGAAATCCAGATCAGCCGACTCCTGAGCATATTGTTGAGGAGA
TGAAACGAGCTGTCGCTGATCCTGAGAATCATAAATATTCGTCTTTTCGCGGCTCATACCGTCTGAAGTCAGCGGCTGCT
GCATTTTACAAAAGAGAATACGGCATTGATCTGGATCCGGAAACCGAAGTCGCTGTATTATTCGGCGGAAAAGCCGGTCT
AGTCGAGCTCCCGCAATGCTTGTTGAATCCCGGTGATACGATTTTAGTTCCCGATCCGGGTTATCCTGATTACTGGTCGG
GTGTCACACTTGCAAAAGCAAAGATGGAAATGATGCCGCTCGTAAAGGACAGAGCGTTTCTCCCTGATTACAGCAGCATA
ACCGCTGAAATAAGGGAACAGGCGAAATTGATGTATTTGAATTATCCGAATAATCCGACTGGAGCTGTTGCTACTTCCGA
GTTCTTTGAAGATACCGTGCGTTTTGCGGCTGAAAACGGAATCTGCGTCGTTCACGATTTTGCTTACGGCGCTGTAGGAT
TTGACGGCTGCAAGCCTTTAAGCTTTTTGCAGACTGAAGGTGCGAAGGATATCGGAATTGAAATTTACACGCTGTCAAAA
ACGTATAATATGGCAGGATGGCGGGTTGGCTTTGCCGTCGGAAACGCTTCGGTCATTGAAGCGATCAATCTTTATCAAGA
CCATATGTTTGTCAGTCTTTTCAGAGCGACTCAGGAGGCTGCAGCAGAGGCACTGTTAGCCGATCAGACGTGTGTGGCAG
AGCAAAATGCCAGGTATGAGAGCAGGAGAAACGCTTGGATCACGGCATGCCGGGAAATCGGCTGGGATGTAACAGCTCCC
GCAGGTTCTTTTTTTGCATGGCTGCCTGTGCCTGAAGGCTATACTTCTGAGCAGTTCTCAGATCTTTTGCTGGAAAAAGC
GAATGTGGCAGTTGCGGCTGGAAACGGCTTCGGTGAATATGGCGAGGGCTACGTCAGAGTCGGACTTTTAACAAGTGAAG
AAAGACTTAAGGAAGCCGCTTATCGAATTGGCAAACTGAACCTGTTTACCCAAAAAAGCATTGACAAGACCTTATAA

Upstream 100 bases:

>100_bases
TTTCATCTTTTTAACACCCCAAATTTTTCTTTACTTTTTTCATTTAACCATATATGATGATGGACTATCATTTCAAGATT
TTTTTAGAAGGTGAGAATAG

Downstream 100 bases:

>100_bases
CGATGGGATAATTCAAAATAATTTATAAAATCTATATTTTCTTATCAAGAGCAGGCAGAGGGACGAGCCCGATGAAGCCC
GGCAACCGACTTATAAAGCA

Product: transaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKFEQSHVLKELPKQFFASLVQKVNRKLAEGHDVINLGQGNPDQPTPEHIVEEMKRAVADPENHKYSSFRGSYRLKSAAA
AFYKREYGIDLDPETEVAVLFGGKAGLVELPQCLLNPGDTILVPDPGYPDYWSGVTLAKAKMEMMPLVKDRAFLPDYSSI
TAEIREQAKLMYLNYPNNPTGAVATSEFFEDTVRFAAENGICVVHDFAYGAVGFDGCKPLSFLQTEGAKDIGIEIYTLSK
TYNMAGWRVGFAVGNASVIEAINLYQDHMFVSLFRATQEAAAEALLADQTCVAEQNARYESRRNAWITACREIGWDVTAP
AGSFFAWLPVPEGYTSEQFSDLLLEKANVAVAAGNGFGEYGEGYVRVGLLTSEERLKEAAYRIGKLNLFTQKSIDKTL

Sequences:

>Translated_398_residues
MKFEQSHVLKELPKQFFASLVQKVNRKLAEGHDVINLGQGNPDQPTPEHIVEEMKRAVADPENHKYSSFRGSYRLKSAAA
AFYKREYGIDLDPETEVAVLFGGKAGLVELPQCLLNPGDTILVPDPGYPDYWSGVTLAKAKMEMMPLVKDRAFLPDYSSI
TAEIREQAKLMYLNYPNNPTGAVATSEFFEDTVRFAAENGICVVHDFAYGAVGFDGCKPLSFLQTEGAKDIGIEIYTLSK
TYNMAGWRVGFAVGNASVIEAINLYQDHMFVSLFRATQEAAAEALLADQTCVAEQNARYESRRNAWITACREIGWDVTAP
AGSFFAWLPVPEGYTSEQFSDLLLEKANVAVAAGNGFGEYGEGYVRVGLLTSEERLKEAAYRIGKLNLFTQKSIDKTL
>Mature_398_residues
MKFEQSHVLKELPKQFFASLVQKVNRKLAEGHDVINLGQGNPDQPTPEHIVEEMKRAVADPENHKYSSFRGSYRLKSAAA
AFYKREYGIDLDPETEVAVLFGGKAGLVELPQCLLNPGDTILVPDPGYPDYWSGVTLAKAKMEMMPLVKDRAFLPDYSSI
TAEIREQAKLMYLNYPNNPTGAVATSEFFEDTVRFAAENGICVVHDFAYGAVGFDGCKPLSFLQTEGAKDIGIEIYTLSK
TYNMAGWRVGFAVGNASVIEAINLYQDHMFVSLFRATQEAAAEALLADQTCVAEQNARYESRRNAWITACREIGWDVTAP
AGSFFAWLPVPEGYTSEQFSDLLLEKANVAVAAGNGFGEYGEGYVRVGLLTSEERLKEAAYRIGKLNLFTQKSIDKTL

Specific function: Catalyzes the formation of methionine from 2-keto-4- methylthiobutyrate (KMTB) (Probable)

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. MtnE subfamily

Homologues:

Organism=Homo sapiens, GI95147551, Length=317, Percent_Identity=25.2365930599369, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI169881279, Length=317, Percent_Identity=25.2365930599369, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI56713256, Length=315, Percent_Identity=24.4444444444444, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI56713254, Length=315, Percent_Identity=24.4444444444444, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI4507369, Length=390, Percent_Identity=20.7692307692308, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1788722, Length=377, Percent_Identity=38.1962864721485, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI1786816, Length=363, Percent_Identity=25.6198347107438, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788627, Length=337, Percent_Identity=26.1127596439169, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71994472, Length=272, Percent_Identity=27.5735294117647, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71994476, Length=272, Percent_Identity=27.5735294117647, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17567369, Length=274, Percent_Identity=23.7226277372263, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17567663, Length=375, Percent_Identity=24.2666666666667, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322401, Length=325, Percent_Identity=23.0769230769231, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28573069, Length=411, Percent_Identity=21.8978102189781, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24646114, Length=411, Percent_Identity=21.8978102189781, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28573067, Length=411, Percent_Identity=21.8978102189781, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28573065, Length=411, Percent_Identity=21.8978102189781, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTNE_BACSU (O31665)

Other databases:

- EMBL:   AL009126
- PIR:   F69863
- RefSeq:   NP_389241.1
- ProteinModelPortal:   O31665
- SMR:   O31665
- EnsemblBacteria:   EBBACT00000000709
- GeneID:   939330
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13580
- NMPDR:   fig|224308.1.peg.1360
- GenoList:   BSU13580
- GeneTree:   EBGT00070000031804
- HOGENOM:   HBG645860
- OMA:   FPAIQDA
- PhylomeDB:   O31665
- ProtClustDB:   PRK08068
- BioCyc:   BSUB:BSU13580-MONOMER
- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: 2.6.1.- [C]

Molecular weight: Translated: 44043; Mature: 44043

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFEQSHVLKELPKQFFASLVQKVNRKLAEGHDVINLGQGNPDQPTPEHIVEEMKRAVAD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHCC
PENHKYSSFRGSYRLKSAAAAFYKREYGIDLDPETEVAVLFGGKAGLVELPQCLLNPGDT
CCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCE
ILVPDPGYPDYWSGVTLAKAKMEMMPLVKDRAFLPDYSSITAEIREQAKLMYLNYPNNPT
EEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEECCCCCC
GAVATSEFFEDTVRFAAENGICVVHDFAYGAVGFDGCKPLSFLQTEGAKDIGIEIYTLSK
CCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEEC
TYNMAGWRVGFAVGNASVIEAINLYQDHMFVSLFRATQEAAAEALLADQTCVAEQNARYE
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCHHH
SRRNAWITACREIGWDVTAPAGSFFAWLPVPEGYTSEQFSDLLLEKANVAVAAGNGFGEY
HHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCEEEEECCCCCCC
GEGYVRVGLLTSEERLKEAAYRIGKLNLFTQKSIDKTL
CCCEEEEEEECCHHHHHHHHHHHCCEEEEEHHHHHCCC
>Mature Secondary Structure
MKFEQSHVLKELPKQFFASLVQKVNRKLAEGHDVINLGQGNPDQPTPEHIVEEMKRAVAD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHCC
PENHKYSSFRGSYRLKSAAAAFYKREYGIDLDPETEVAVLFGGKAGLVELPQCLLNPGDT
CCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCE
ILVPDPGYPDYWSGVTLAKAKMEMMPLVKDRAFLPDYSSITAEIREQAKLMYLNYPNNPT
EEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEECCCCCC
GAVATSEFFEDTVRFAAENGICVVHDFAYGAVGFDGCKPLSFLQTEGAKDIGIEIYTLSK
CCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEEC
TYNMAGWRVGFAVGNASVIEAINLYQDHMFVSLFRATQEAAAEALLADQTCVAEQNARYE
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCHHH
SRRNAWITACREIGWDVTAPAGSFFAWLPVPEGYTSEQFSDLLLEKANVAVAAGNGFGEY
HHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCEEEEECCCCCCC
GEGYVRVGLLTSEERLKEAAYRIGKLNLFTQKSIDKTL
CCCEEEEEEECCHHHHHHHHHHHCCEEEEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 12022921