Definition Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession NC_000964
Length 4,215,606

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The map label for this gene is kinE

Identifier: 16078417

GI number: 16078417

Start: 1419213

End: 1421429

Strand: Direct

Name: kinE

Synonym: BSU13530

Alternate gene names: 16078417

Gene position: 1419213-1421429 (Clockwise)

Preceding gene: 16078415

Following gene: 16078418

Centisome position: 33.67

GC content: 40.87

Gene sequence:

>2217_bases
ATGGAAACTCTTGGTGTCCAGACAAACTCGGAGCTGCGAGAGGAACTGAATCGCCTCAAAGAAGAAAACGCGCGACTGAA
AAAGGAATTGAATCAGCATCAAGTGATTGTCAACAATACGCTCGATGCTATTTTTATTTGTGATAACGAAATGCGGATTG
TTCAGGCAAATGAAGCAACAGAAAGGATGCTTCAAGTTGACTCGGAAGATTTGAAAAAGCGTTCTGTTTTGGATTTTTTG
TTTTCAATCCCAAAGGATGAACTGAATCTTTCCGTAAAGAAGTTTTTTAAAAAAGGCTTTCTGTGGAAGGAGGTTCCGAT
CAGGCTTGATTGCGGGGCAACAAAATACATAGAGTTTCTGGCCAAAAGAGGTATTGGTGAGGATTTCTTTTTTGTTGTCA
TGAGGGACATATCTTCTAAGAAGATTCTGGAGCGGGAATTCTCTATGAATGAACAGCTTTTTAAGGACTTGTTTGACAGA
GCAGTTGACGGAATCGTCCTGTTTGATAAAGATGGCGGTTTTATTGATGCAAATTTGTCCTTTTGTAAAAGCTTTGAAAT
CAATCATAATGAGCTGTCTCATTTATCTTTGTATGAGTTCATAGACAGCGGCTCACGAAAAGATTTCGACAATATCTGGA
AGGCGCTCAACCGCAAGGGAAAAGCGAAGGGGGAGCTGCCTGTCAAGCTTCGTTCAGGAGTTCAAAAACTATTTGAATTC
ACGATTACATCAAACATCATCAGCGGTTTTTATATGTCCATTATGAGGGATATCACAGAGAAGCGGTCTATGGAGCTGCA
GCTCTTTAAAAGTGAGGAGCGCTTCAGAGAAATCTTTGAAAATGCAATGGACGCCATCATCATCTGGTCGAATGACGGAA
GAATCGTAAAAGCGAATCAATCAGCCTGTAAAATTTTTGAGCTGCCGATGAATTTGCTGTTGAAAAGAAAGCTGTGTGAT
TTTCTCGTTGACTCGCAGCAGAAATACAGCATAACGAAGAGGAAATACGCAAAATACGGAGAGATCAGAGAAGAGCTGCT
GTTTCAAATGGGGAACGGCCAATTCAAAGAGCTTGAATTTACTTCAAAACGGACAATCCTTGAAAATCAGCATTTGACGA
TCTTAAGAAATGTAAGCGACAGAAAGCGGATGGAAAAGGAGCTTCGGGAAAGCGAGCTGAAATTCAGAAAAGTGTTCAAC
GGATCAATGGACGGCAATGTATTGTTCGACAATCAATACAGGATCATCGACGCCAACCCGTTAGCAAGCCATATTTTGGG
CCTGTCACATGAAGAGATCAAACAGCACAGCCTGTTAGATATTATTTCTGCTTATGAAATAGAAAATTTGGCATCACCGG
CAAGGCAGATTAATTTTGATGAAATGGACAACGAAATTCCATTTTTGCTGAGCAGCGGTGATAACAGAAAACTCGAATTC
TCATTTAAACGGAATATTATTCAAAATATGAACTTGGCTATCTTTAAAGACGTAACAGAGCGAAAAGAATTGGAGGAGCG
CCTGCGCAAATCAGATACCCTTCATGTTGTCGGCGAACTCGCAGCCGGTATTGCTCATGAAATCAGAAATCCGATGACTG
CTCTTAAAGGCTTCATTCAGCTTCTGAAAGGGAGTGTCGAGGGAGACTACGCCCTTTACTTCAACGTGATCACCTCAGAA
CTAAAGCGCATCGAATCCATCATCACCGAATTCTTGATTTTAGCCAAGCCCCAGGCCATTATGTATGAGGAAAAACATGT
TACCCAAATTATGAGGGACACGATAGACCTTCTAAATGCGCAAGCCAATCTCAGCAATGTACAAATGCAACTTGATTTAA
TTGACGACATTCCCCCGATTTACTGTGAACCGAATCAGCTGAAACAGGTTTTTATCAATATATTAAAAAACGCCATCGAA
GTGATGCCTGACGGCGGAAATATTTTTGTAACGATAAAGGCATTGGATCAAGATCATGTTCTAATCTCCTTAAAAGACGA
GGGAATCGGAATGACAGAAGATAAATTAAAGCGACTTGGTGAACCGTTTTATACGACAAAGGAACGGGGAACTGGACTTG
GGCTTATGGTAAGCTATAAAATTATTGAAGAACATCAAGGCGAGATTATGGTAGAAAGTGAAGAAGGGAAGGGCACCGTT
TTCCATATTACGCTTCCTGTCAGACAGAATGCTGAAGAAAGAAGGAATGATGAATGA

Upstream 100 bases:

>100_bases
ATGACGCTGAAATTTTCTGAAATTTATAGGGCAAATGAAGGAAGTTCCCGCATTTTGTAGAAATATTGAGGGATAGGTTT
TGAAGAAGGAGTGTACATGC

Downstream 100 bases:

>100_bases
ACTACTATACGACAGCCGAAACGCCGCTCGGTGAACTTATCATTGCCGAAGAGGAGGACCGGATCACTCGTCTATTTCTC
AGTCAGGAAGATTGGGTGGA

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E

Number of amino acids: Translated: 738; Mature: 738

Protein sequence:

>738_residues
METLGVQTNSELREELNRLKEENARLKKELNQHQVIVNNTLDAIFICDNEMRIVQANEATERMLQVDSEDLKKRSVLDFL
FSIPKDELNLSVKKFFKKGFLWKEVPIRLDCGATKYIEFLAKRGIGEDFFFVVMRDISSKKILEREFSMNEQLFKDLFDR
AVDGIVLFDKDGGFIDANLSFCKSFEINHNELSHLSLYEFIDSGSRKDFDNIWKALNRKGKAKGELPVKLRSGVQKLFEF
TITSNIISGFYMSIMRDITEKRSMELQLFKSEERFREIFENAMDAIIIWSNDGRIVKANQSACKIFELPMNLLLKRKLCD
FLVDSQQKYSITKRKYAKYGEIREELLFQMGNGQFKELEFTSKRTILENQHLTILRNVSDRKRMEKELRESELKFRKVFN
GSMDGNVLFDNQYRIIDANPLASHILGLSHEEIKQHSLLDIISAYEIENLASPARQINFDEMDNEIPFLLSSGDNRKLEF
SFKRNIIQNMNLAIFKDVTERKELEERLRKSDTLHVVGELAAGIAHEIRNPMTALKGFIQLLKGSVEGDYALYFNVITSE
LKRIESIITEFLILAKPQAIMYEEKHVTQIMRDTIDLLNAQANLSNVQMQLDLIDDIPPIYCEPNQLKQVFINILKNAIE
VMPDGGNIFVTIKALDQDHVLISLKDEGIGMTEDKLKRLGEPFYTTKERGTGLGLMVSYKIIEEHQGEIMVESEEGKGTV
FHITLPVRQNAEERRNDE

Sequences:

>Translated_738_residues
METLGVQTNSELREELNRLKEENARLKKELNQHQVIVNNTLDAIFICDNEMRIVQANEATERMLQVDSEDLKKRSVLDFL
FSIPKDELNLSVKKFFKKGFLWKEVPIRLDCGATKYIEFLAKRGIGEDFFFVVMRDISSKKILEREFSMNEQLFKDLFDR
AVDGIVLFDKDGGFIDANLSFCKSFEINHNELSHLSLYEFIDSGSRKDFDNIWKALNRKGKAKGELPVKLRSGVQKLFEF
TITSNIISGFYMSIMRDITEKRSMELQLFKSEERFREIFENAMDAIIIWSNDGRIVKANQSACKIFELPMNLLLKRKLCD
FLVDSQQKYSITKRKYAKYGEIREELLFQMGNGQFKELEFTSKRTILENQHLTILRNVSDRKRMEKELRESELKFRKVFN
GSMDGNVLFDNQYRIIDANPLASHILGLSHEEIKQHSLLDIISAYEIENLASPARQINFDEMDNEIPFLLSSGDNRKLEF
SFKRNIIQNMNLAIFKDVTERKELEERLRKSDTLHVVGELAAGIAHEIRNPMTALKGFIQLLKGSVEGDYALYFNVITSE
LKRIESIITEFLILAKPQAIMYEEKHVTQIMRDTIDLLNAQANLSNVQMQLDLIDDIPPIYCEPNQLKQVFINILKNAIE
VMPDGGNIFVTIKALDQDHVLISLKDEGIGMTEDKLKRLGEPFYTTKERGTGLGLMVSYKIIEEHQGEIMVESEEGKGTV
FHITLPVRQNAEERRNDE
>Mature_738_residues
METLGVQTNSELREELNRLKEENARLKKELNQHQVIVNNTLDAIFICDNEMRIVQANEATERMLQVDSEDLKKRSVLDFL
FSIPKDELNLSVKKFFKKGFLWKEVPIRLDCGATKYIEFLAKRGIGEDFFFVVMRDISSKKILEREFSMNEQLFKDLFDR
AVDGIVLFDKDGGFIDANLSFCKSFEINHNELSHLSLYEFIDSGSRKDFDNIWKALNRKGKAKGELPVKLRSGVQKLFEF
TITSNIISGFYMSIMRDITEKRSMELQLFKSEERFREIFENAMDAIIIWSNDGRIVKANQSACKIFELPMNLLLKRKLCD
FLVDSQQKYSITKRKYAKYGEIREELLFQMGNGQFKELEFTSKRTILENQHLTILRNVSDRKRMEKELRESELKFRKVFN
GSMDGNVLFDNQYRIIDANPLASHILGLSHEEIKQHSLLDIISAYEIENLASPARQINFDEMDNEIPFLLSSGDNRKLEF
SFKRNIIQNMNLAIFKDVTERKELEERLRKSDTLHVVGELAAGIAHEIRNPMTALKGFIQLLKGSVEGDYALYFNVITSE
LKRIESIITEFLILAKPQAIMYEEKHVTQIMRDTIDLLNAQANLSNVQMQLDLIDDIPPIYCEPNQLKQVFINILKNAIE
VMPDGGNIFVTIKALDQDHVLISLKDEGIGMTEDKLKRLGEPFYTTKERGTGLGLMVSYKIIEEHQGEIMVESEEGKGTV
FHITLPVRQNAEERRNDE

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains

Homologues:

Organism=Escherichia coli, GI1788549, Length=364, Percent_Identity=30.7692307692308, Blast_Score=170, Evalue=4e-43,
Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=34.4978165938865, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1790300, Length=345, Percent_Identity=25.2173913043478, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI1786600, Length=382, Percent_Identity=25.6544502617801, Blast_Score=93, Evalue=7e-20,
Organism=Escherichia coli, GI145693157, Length=317, Percent_Identity=28.391167192429, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788713, Length=391, Percent_Identity=24.8081841432225, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789149, Length=239, Percent_Identity=27.6150627615063, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790346, Length=217, Percent_Identity=27.1889400921659, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI48994928, Length=257, Percent_Identity=25.2918287937743, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI87081816, Length=224, Percent_Identity=25.8928571428571, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1788393, Length=227, Percent_Identity=23.7885462555066, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1786783, Length=220, Percent_Identity=28.1818181818182, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1790861, Length=211, Percent_Identity=23.696682464455, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KINE_BACSU (O31661)

Other databases:

- EMBL:   AL009126
- PIR:   B69863
- RefSeq:   NP_389236.1
- ProteinModelPortal:   O31661
- EnsemblBacteria:   EBBACT00000003851
- GeneID:   939349
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13530
- NMPDR:   fig|224308.1.peg.1355
- GenoList:   BSU13530
- GeneTree:   EBGT00070000031987
- HOGENOM:   HBG313687
- OMA:   IMRDITE
- PhylomeDB:   O31661
- ProtClustDB:   CLSK887190
- BioCyc:   BSUB:BSU13530-MONOMER
- GO:   GO:0016020
- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- Gene3D:   G3DSA:3.30.565.10
- PRINTS:   PR00344
- SMART:   SM00387
- SMART:   SM00388
- SMART:   SM00091
- TIGRFAMs:   TIGR00229

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS; SSF55874 ATP_bd_ATPase; SSF47384 His_kin_homodim

EC number: =2.7.13.3

Molecular weight: Translated: 85515; Mature: 85515

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50109 HIS_KIN; PS50112 PAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
METLGVQTNSELREELNRLKEENARLKKELNQHQVIVNNTLDAIFICDNEMRIVQANEAT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEEEEECCHHH
ERMLQVDSEDLKKRSVLDFLFSIPKDELNLSVKKFFKKGFLWKEVPIRLDCGATKYIEFL
HHHHHCCHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHCCCEECCCEEEECCHHHHHHHH
AKRGIGEDFFFVVMRDISSKKILEREFSMNEQLFKDLFDRAVDGIVLFDKDGGFIDANLS
HHCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCEEECCHH
FCKSFEINHNELSHLSLYEFIDSGSRKDFDNIWKALNRKGKAKGELPVKLRSGVQKLFEF
HHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
TITSNIISGFYMSIMRDITEKRSMELQLFKSEERFREIFENAMDAIIIWSNDGRIVKANQ
HHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHCCCEEEEECCCCEEEECCC
SACKIFELPMNLLLKRKLCDFLVDSQQKYSITKRKYAKYGEIREELLFQMGNGQFKELEF
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEH
TSKRTILENQHLTILRNVSDRKRMEKELRESELKFRKVFNGSMDGNVLFDNQYRIIDANP
HHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCH
LASHILGLSHEEIKQHSLLDIISAYEIENLASPARQINFDEMDNEIPFLLSSGDNRKLEF
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHCCCCCCEEEECCCCCEEEE
SFKRNIIQNMNLAIFKDVTERKELEERLRKSDTLHVVGELAAGIAHEIRNPMTALKGFIQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHH
LLKGSVEGDYALYFNVITSELKRIESIITEFLILAKPQAIMYEEKHVTQIMRDTIDLLNA
HHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
QANLSNVQMQLDLIDDIPPIYCEPNQLKQVFINILKNAIEVMPDGGNIFVTIKALDQDHV
CCCCCCEEEEEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCE
LISLKDEGIGMTEDKLKRLGEPFYTTKERGTGLGLMVSYKIIEEHQGEIMVESEEGKGTV
EEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEEEHHCCCCCEEEEECCCCEEE
FHITLPVRQNAEERRNDE
EEEEECCCCCHHHCCCCC
>Mature Secondary Structure
METLGVQTNSELREELNRLKEENARLKKELNQHQVIVNNTLDAIFICDNEMRIVQANEAT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEEEEECCHHH
ERMLQVDSEDLKKRSVLDFLFSIPKDELNLSVKKFFKKGFLWKEVPIRLDCGATKYIEFL
HHHHHCCHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHCCCEECCCEEEECCHHHHHHHH
AKRGIGEDFFFVVMRDISSKKILEREFSMNEQLFKDLFDRAVDGIVLFDKDGGFIDANLS
HHCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCEEECCHH
FCKSFEINHNELSHLSLYEFIDSGSRKDFDNIWKALNRKGKAKGELPVKLRSGVQKLFEF
HHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
TITSNIISGFYMSIMRDITEKRSMELQLFKSEERFREIFENAMDAIIIWSNDGRIVKANQ
HHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHCCCEEEEECCCCEEEECCC
SACKIFELPMNLLLKRKLCDFLVDSQQKYSITKRKYAKYGEIREELLFQMGNGQFKELEF
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEH
TSKRTILENQHLTILRNVSDRKRMEKELRESELKFRKVFNGSMDGNVLFDNQYRIIDANP
HHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCH
LASHILGLSHEEIKQHSLLDIISAYEIENLASPARQINFDEMDNEIPFLLSSGDNRKLEF
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHCCCCCCEEEECCCCCEEEE
SFKRNIIQNMNLAIFKDVTERKELEERLRKSDTLHVVGELAAGIAHEIRNPMTALKGFIQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHH
LLKGSVEGDYALYFNVITSELKRIESIITEFLILAKPQAIMYEEKHVTQIMRDTIDLLNA
HHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
QANLSNVQMQLDLIDDIPPIYCEPNQLKQVFINILKNAIEVMPDGGNIFVTIKALDQDHV
CCCCCCEEEEEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCE
LISLKDEGIGMTEDKLKRLGEPFYTTKERGTGLGLMVSYKIIEEHQGEIMVESEEGKGTV
EEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEEEHHCCCCCEEEEECCCCEEE
FHITLPVRQNAEERRNDE
EEEEECCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377