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The map label for this gene is ligD

Identifier: 16078405

GI number: 16078405

Start: 1404518

End: 1406353

Strand: Reverse

Name: ligD

Synonym: BSU13400

Alternate gene names: 16078405

Gene position: 1406353-1404518 (Counterclockwise)

Preceding gene: 16078406

Following gene: 16078402

Centisome position: 33.36

GC content: 45.1

Gene sequence:

>1836_bases
ATGGCGTTTACCATGCAGCCGGTCTTAACTTCGTCACCGCCAATCGGAGCAGAATGGCGTTACGAAGTAAAATATGATGG
GTACCGCTGTATTCTCCGCATCCATTCTTCAGGTGTGACCTTAACCAGCAGAAATGGTGTGGAGCTCAGCAGCACCTTCC
CTGAAATCACACAATTCGCCAAAACTGCCTTTCAGCACCTAGAAAAAGAGCTTCCGCTGACACTTGACGGTGAAATCGTA
TGTTTGGTGAATCCCTGTCGTGCAGATTTTGAACACCTTCAAGTGCGGGGGCGTTTGAAAAGGCCAGACAAGATTCAGGA
ATCGGCAAACGCGCGGCCGTGCTGCTTTCTCGCCTTTGATTTGTTAGAGCGAAGCGGAGAAGATGTAACGCTTCTTTCGT
ATTTGGACCGCAAAAAATCGCTGCGTGAACTCATATCCGCAGCAAAGCTTCCTGCATCTCCAGATCCTTACGCAAAAGAA
ACCATTCAATCCATTCCCTGTTATGATCATTTTGATCAACTCTGGGAGATGGTAATAAAATATGACGGAGAAGGAATTGT
AGCGAAGAAAACAAACAGCAAATGGCTTGAAAAGAAGCGGTCGTCCGATTGGCTCAAATATAAAAACTTTAAACAAGCTT
ATGTTTGCATAACAGGGTTCAACCCTAACAACGGGTTTCTGACGGTATCGGTGTTAAAAAACGGAATAATGACACCGATT
GCCTCTGTCTCGCACGGAATGCGTGATGAGGAAAAAAGCGCTATACGCGAGATTATGGAACAGCACGGACATCAAACACC
CTCAGGCGAATTTACGCTTGAACCTTCGATTTGTGCAGCTGTTCAATACTTAACAATTCTCCAAGGCACATTAAGAGAGG
TATCGTTTATCGGTTTCGAATTCCAAATGGACTGGACCGAATGCACGTATGCGCAAGTCATACGACACTCCAAACCGGTT
CACCCAAAGCTCCAGTTCACAAGCCTGGATAAAATCATATTTGAAAAGAATAAAAAAACAAAAGAAGATTTTATTCAGTA
TATGATTGAAGTCAGTGACTACCTGCTCCCATTTCTGAAAAACCGGGCTGTCACCGTCATCCGCTATCCGCATGGATCTA
GGAGCGAATCATTTTTCCAAAAAAACAAGCCGGACTATGCGCCGGATTTTGTTCAGTCTTTCTATGATGGAAGCCATGAA
CATATTGTCTGTGAGGATATGTCTACATTGCTATGGCTTTGCAACCAGCTTGCTTTGGAGTTTCACGTTCCTTTTCAAAC
CATTAAAAGCAGGCGTCCGGCTGAGATTGTCATTGATTTAGACCCGCCTTCGCGAGATGACTTTCTTATGGCCGTGCAGG
CCGCAAATGAACTGAAGCGGCTGTTGGATTCCTTTGGCATCACATCGTACCCGAAGCTGTCCGGCAACAAAGGCATCCAG
CTTTATATTCCCTTGTCTCCCGAGGCATTTACGTATGAAGAAACACGCCAATTTACGCAGCTGATCGCAGAGTACTGCAC
CAATGCATTTCCCGAGCTCTTTACCACTGAACGCTTGATTAAAAACAGACACTGCAAATTGTATCTTGATTATTTGCAGC
ATGCAGAAGGAAAAACGATTATCTGTCCCTATTCAACGAGAGGAAATGAGCTTGGGACTGTTGCAGCCCCGCTCTACTGG
CATGAGGTGCAATCATCTTTAACACCCGCTCTGTTTACAATTGATACGGTCATCGATCGAATTAAGAAGCAGGGCTGCCC
ATTTTTTGATTTTTACCGCAACCCGCAAGACGAACCGCTTAGCGCTATATTGCATCAGTTGAAGAAAAAGAGCTGA

Upstream 100 bases:

>100_bases
GACACCGGCTGCCGCACCTGCACAAGCCGCTGAACCGAAAGGAGCCGGAGATAAAAAACAAAAAACAACTAGAAAAAAAG
CCTCCGGCACATCATAGCGC

Downstream 100 bases:

>100_bases
CTAATGAAGTCAGCTCTTTCCGTGTTTAACTGTACTTTTTTTCTAACCGCTCTTTTTCCTGGCGCTGAAGCGGGCTCAGC
TGTTCATTATACAAATCAAT

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 611; Mature: 610

Protein sequence:

>611_residues
MAFTMQPVLTSSPPIGAEWRYEVKYDGYRCILRIHSSGVTLTSRNGVELSSTFPEITQFAKTAFQHLEKELPLTLDGEIV
CLVNPCRADFEHLQVRGRLKRPDKIQESANARPCCFLAFDLLERSGEDVTLLSYLDRKKSLRELISAAKLPASPDPYAKE
TIQSIPCYDHFDQLWEMVIKYDGEGIVAKKTNSKWLEKKRSSDWLKYKNFKQAYVCITGFNPNNGFLTVSVLKNGIMTPI
ASVSHGMRDEEKSAIREIMEQHGHQTPSGEFTLEPSICAAVQYLTILQGTLREVSFIGFEFQMDWTECTYAQVIRHSKPV
HPKLQFTSLDKIIFEKNKKTKEDFIQYMIEVSDYLLPFLKNRAVTVIRYPHGSRSESFFQKNKPDYAPDFVQSFYDGSHE
HIVCEDMSTLLWLCNQLALEFHVPFQTIKSRRPAEIVIDLDPPSRDDFLMAVQAANELKRLLDSFGITSYPKLSGNKGIQ
LYIPLSPEAFTYEETRQFTQLIAEYCTNAFPELFTTERLIKNRHCKLYLDYLQHAEGKTIICPYSTRGNELGTVAAPLYW
HEVQSSLTPALFTIDTVIDRIKKQGCPFFDFYRNPQDEPLSAILHQLKKKS

Sequences:

>Translated_611_residues
MAFTMQPVLTSSPPIGAEWRYEVKYDGYRCILRIHSSGVTLTSRNGVELSSTFPEITQFAKTAFQHLEKELPLTLDGEIV
CLVNPCRADFEHLQVRGRLKRPDKIQESANARPCCFLAFDLLERSGEDVTLLSYLDRKKSLRELISAAKLPASPDPYAKE
TIQSIPCYDHFDQLWEMVIKYDGEGIVAKKTNSKWLEKKRSSDWLKYKNFKQAYVCITGFNPNNGFLTVSVLKNGIMTPI
ASVSHGMRDEEKSAIREIMEQHGHQTPSGEFTLEPSICAAVQYLTILQGTLREVSFIGFEFQMDWTECTYAQVIRHSKPV
HPKLQFTSLDKIIFEKNKKTKEDFIQYMIEVSDYLLPFLKNRAVTVIRYPHGSRSESFFQKNKPDYAPDFVQSFYDGSHE
HIVCEDMSTLLWLCNQLALEFHVPFQTIKSRRPAEIVIDLDPPSRDDFLMAVQAANELKRLLDSFGITSYPKLSGNKGIQ
LYIPLSPEAFTYEETRQFTQLIAEYCTNAFPELFTTERLIKNRHCKLYLDYLQHAEGKTIICPYSTRGNELGTVAAPLYW
HEVQSSLTPALFTIDTVIDRIKKQGCPFFDFYRNPQDEPLSAILHQLKKKS
>Mature_610_residues
AFTMQPVLTSSPPIGAEWRYEVKYDGYRCILRIHSSGVTLTSRNGVELSSTFPEITQFAKTAFQHLEKELPLTLDGEIVC
LVNPCRADFEHLQVRGRLKRPDKIQESANARPCCFLAFDLLERSGEDVTLLSYLDRKKSLRELISAAKLPASPDPYAKET
IQSIPCYDHFDQLWEMVIKYDGEGIVAKKTNSKWLEKKRSSDWLKYKNFKQAYVCITGFNPNNGFLTVSVLKNGIMTPIA
SVSHGMRDEEKSAIREIMEQHGHQTPSGEFTLEPSICAAVQYLTILQGTLREVSFIGFEFQMDWTECTYAQVIRHSKPVH
PKLQFTSLDKIIFEKNKKTKEDFIQYMIEVSDYLLPFLKNRAVTVIRYPHGSRSESFFQKNKPDYAPDFVQSFYDGSHEH
IVCEDMSTLLWLCNQLALEFHVPFQTIKSRRPAEIVIDLDPPSRDDFLMAVQAANELKRLLDSFGITSYPKLSGNKGIQL
YIPLSPEAFTYEETRQFTQLIAEYCTNAFPELFTTERLIKNRHCKLYLDYLQHAEGKTIICPYSTRGNELGTVAAPLYWH
EVQSSLTPALFTIDTVIDRIKKQGCPFFDFYRNPQDEPLSAILHQLKKKS

Specific function: Probably involved in the repair of DNA double-strand breaks by non-homologous-end joining (NHEJ) during spore germination

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YKOU_BACSU (O34398)

Other databases:

- EMBL:   AL009126
- PIR:   G69860
- RefSeq:   NP_389223.1
- HSSP:   P71571
- ProteinModelPortal:   O34398
- SMR:   O34398
- EnsemblBacteria:   EBBACT00000001060
- GeneID:   939372
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13400
- NMPDR:   fig|224308.1.peg.1342
- GenoList:   BSU13400
- GeneTree:   EBGT00050000000591
- HOGENOM:   HBG337951
- OMA:   LAMMVED
- ProtClustDB:   PRK09633
- BioCyc:   BSUB:BSU13400-MONOMER
- InterPro:   IPR012310
- InterPro:   IPR014146
- InterPro:   IPR014145
- InterPro:   IPR014143
- TIGRFAMs:   TIGR02778
- TIGRFAMs:   TIGR02779
- TIGRFAMs:   TIGR02776

Pfam domain/function: PF01068 DNA_ligase_A_M

EC number: =6.5.1.1

Molecular weight: Translated: 70205; Mature: 70074

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00697 DNA_LIGASE_A1; PS00333 DNA_LIGASE_A2; PS50160 DNA_LIGASE_A3

Important sites: ACT_SITE 24-24 BINDING 22-22 BINDING 29-29 BINDING 44-44 BINDING 78-78 BINDING 119-119 BINDING 200-200 BINDING 206-206

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFTMQPVLTSSPPIGAEWRYEVKYDGYRCILRIHSSGVTLTSRNGVELSSTFPEITQFA
CCEECCCHHCCCCCCCCCEEEEEEECCEEEEEEEECCCEEEECCCCCCCCCCCHHHHHHH
KTAFQHLEKELPLTLDGEIVCLVNPCRADFEHLQVRGRLKRPDKIQESANARPCCFLAFD
HHHHHHHHHHCCEEECCCEEEEECCHHCCHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHH
LLERSGEDVTLLSYLDRKKSLRELISAAKLPASPDPYAKETIQSIPCYDHFDQLWEMVIK
HHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
YDGEGIVAKKTNSKWLEKKRSSDWLKYKNFKQAYVCITGFNPNNGFLTVSVLKNGIMTPI
CCCCEEEEECCCHHHHHHHCCCCCHHHCCCCEEEEEEEEECCCCCEEEHHHHHCCCHHHH
ASVSHGMRDEEKSAIREIMEQHGHQTPSGEFTLEPSICAAVQYLTILQGTLREVSFIGFE
HHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHEEEEE
FQMDWTECTYAQVIRHSKPVHPKLQFTSLDKIIFEKNKKTKEDFIQYMIEVSDYLLPFLK
EEECHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
NRAVTVIRYPHGSRSESFFQKNKPDYAPDFVQSFYDGSHEHIVCEDMSTLLWLCNQLALE
CCCEEEEECCCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHE
FHVPFQTIKSRRPAEIVIDLDPPSRDDFLMAVQAANELKRLLDSFGITSYPKLSGNKGIQ
EECCHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
LYIPLSPEAFTYEETRQFTQLIAEYCTNAFPELFTTERLIKNRHCKLYLDYLQHAEGKTI
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCEE
ICPYSTRGNELGTVAAPLYWHEVQSSLTPALFTIDTVIDRIKKQGCPFFDFYRNPQDEPL
EEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHH
SAILHQLKKKS
HHHHHHHHCCC
>Mature Secondary Structure 
AFTMQPVLTSSPPIGAEWRYEVKYDGYRCILRIHSSGVTLTSRNGVELSSTFPEITQFA
CEECCCHHCCCCCCCCCEEEEEEECCEEEEEEEECCCEEEECCCCCCCCCCCHHHHHHH
KTAFQHLEKELPLTLDGEIVCLVNPCRADFEHLQVRGRLKRPDKIQESANARPCCFLAFD
HHHHHHHHHHCCEEECCCEEEEECCHHCCHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHH
LLERSGEDVTLLSYLDRKKSLRELISAAKLPASPDPYAKETIQSIPCYDHFDQLWEMVIK
HHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
YDGEGIVAKKTNSKWLEKKRSSDWLKYKNFKQAYVCITGFNPNNGFLTVSVLKNGIMTPI
CCCCEEEEECCCHHHHHHHCCCCCHHHCCCCEEEEEEEEECCCCCEEEHHHHHCCCHHHH
ASVSHGMRDEEKSAIREIMEQHGHQTPSGEFTLEPSICAAVQYLTILQGTLREVSFIGFE
HHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHEEEEE
FQMDWTECTYAQVIRHSKPVHPKLQFTSLDKIIFEKNKKTKEDFIQYMIEVSDYLLPFLK
EEECHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
NRAVTVIRYPHGSRSESFFQKNKPDYAPDFVQSFYDGSHEHIVCEDMSTLLWLCNQLALE
CCCEEEEECCCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHE
FHVPFQTIKSRRPAEIVIDLDPPSRDDFLMAVQAANELKRLLDSFGITSYPKLSGNKGIQ
EECCHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
LYIPLSPEAFTYEETRQFTQLIAEYCTNAFPELFTTERLIKNRHCKLYLDYLQHAEGKTI
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCEE
ICPYSTRGNELGTVAAPLYWHEVQSSLTPALFTIDTVIDRIKKQGCPFFDFYRNPQDEPL
EEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHH
SAILHQLKKKS
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377