Definition Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession NC_000964
Length 4,215,606

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The map label for this gene is yhjM

Identifier: 16078120

GI number: 16078120

Start: 1129715

End: 1130704

Strand: Direct

Name: yhjM

Synonym: BSU10560

Alternate gene names: 16078120

Gene position: 1129715-1130704 (Clockwise)

Preceding gene: 16078115

Following gene: 16078123

Centisome position: 26.8

GC content: 35.56

Gene sequence:

>990_bases
ATGCCTACAATTGATGAAATAGCAAAATTATGCAATGTGTCTAAAACCACAGTGTCAAGAGTATTGAATAATCATCCTTA
TGTGTCGAAAGAAAAAAGAGATATGATCTTAAAGGCTATTAACGAACTTGACTACACTCCCAATTATTTAGCTCGGAATT
TTAGGAGAAATAAGACACAAACGATAGCTTTATCGGTTCCGAGTATCGACCATCCATTTTTTGCTCAATTAATAAAAGGG
GTCTCTCATGAAGTACTGTTTAAGAATTACAAGGTAATTGTATTTCAGACCTTTTATGACAAACAGACGGAGCTAGAATT
ACTAGAGTTATTGAAGCATAAAGAAGTGGATGGCATTATTCTTGGGACTTTAGAAAATGAATGGGATCAGATATCCCCTT
TCTTAAAGTATGGTCCTATCTTATTATGTAATGAATATCATCACTCAGCGGACATCACGATAATTGGGTATGATGAATTT
GAAGCCGCATACATGGGGGTAGTACATTTAATTGAAAGAGGGCATAAAAAAATTGGGTTTTGTTTTGATACTCCATATAG
CGAAGCTCAATGTCAGAGGAAAGAGGGTTATTTGAAAGCTTTACAAGATTACAATTTACATCATAGAAGCGAATGGATAT
TCGGCGAAATGTTTAATATCGAGGATGGCTTTCGTGTTTTTCATAAGATAAAGGATCTAAAGGACAGACCCTCAGCAATA
TTTACAGGAAATGATCAAGTCGCCGCAGGGATTATTAAACAGGCGATGAAAAATGGCTTTAAAGTTCCAGAAGACTTAGC
TGTAATTGGTTTTGACAACCAATTGATTTGCCAAGTCGTTACTCCGACCATAACAACAATTGATATCCCCGTAATAGAAT
TAGGCCAACAGGCTGTGTTGAAAATCATCGAGTCCATTTCGGGTAATGCCTCTTTAAACAGAAGAATTATTAAACTGCCA
ACAAAGCTTATTATAAGAGAATCAACGTAA

Upstream 100 bases:

>100_bases
CAGTACCTCCAATGATTTTATAATCTAAAAATTTATTACAAATTTATCCTAGTTTCTGAAATCGGTAACATGTCAACAAC
TTTTTTAAGGAAGGGAAAAT

Downstream 100 bases:

>100_bases
CGATAGATTTCCCTTTAACATTTTATATCTTTATATAAGAAAAAGAGGTTATTCATTTTAACCTCTTTTTCAATCGTAAG
TCGTCAATTATTTCTTTCAT

Product: transcriptional regulator of the ntd operon; (LacI family)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MPTIDEIAKLCNVSKTTVSRVLNNHPYVSKEKRDMILKAINELDYTPNYLARNFRRNKTQTIALSVPSIDHPFFAQLIKG
VSHEVLFKNYKVIVFQTFYDKQTELELLELLKHKEVDGIILGTLENEWDQISPFLKYGPILLCNEYHHSADITIIGYDEF
EAAYMGVVHLIERGHKKIGFCFDTPYSEAQCQRKEGYLKALQDYNLHHRSEWIFGEMFNIEDGFRVFHKIKDLKDRPSAI
FTGNDQVAAGIIKQAMKNGFKVPEDLAVIGFDNQLICQVVTPTITTIDIPVIELGQQAVLKIIESISGNASLNRRIIKLP
TKLIIREST

Sequences:

>Translated_329_residues
MPTIDEIAKLCNVSKTTVSRVLNNHPYVSKEKRDMILKAINELDYTPNYLARNFRRNKTQTIALSVPSIDHPFFAQLIKG
VSHEVLFKNYKVIVFQTFYDKQTELELLELLKHKEVDGIILGTLENEWDQISPFLKYGPILLCNEYHHSADITIIGYDEF
EAAYMGVVHLIERGHKKIGFCFDTPYSEAQCQRKEGYLKALQDYNLHHRSEWIFGEMFNIEDGFRVFHKIKDLKDRPSAI
FTGNDQVAAGIIKQAMKNGFKVPEDLAVIGFDNQLICQVVTPTITTIDIPVIELGQQAVLKIIESISGNASLNRRIIKLP
TKLIIREST
>Mature_328_residues
PTIDEIAKLCNVSKTTVSRVLNNHPYVSKEKRDMILKAINELDYTPNYLARNFRRNKTQTIALSVPSIDHPFFAQLIKGV
SHEVLFKNYKVIVFQTFYDKQTELELLELLKHKEVDGIILGTLENEWDQISPFLKYGPILLCNEYHHSADITIIGYDEFE
AAYMGVVHLIERGHKKIGFCFDTPYSEAQCQRKEGYLKALQDYNLHHRSEWIFGEMFNIEDGFRVFHKIKDLKDRPSAIF
TGNDQVAAGIIKQAMKNGFKVPEDLAVIGFDNQLICQVVTPTITTIDIPVIELGQQAVLKIIESISGNASLNRRIIKLPT
KLIIREST

Specific function: Positively regulates the ntdABC operon and negatively regulates its own transcription. Binds to NTD to induce ntdABC transcription

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1790369, Length=330, Percent_Identity=33.030303030303, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1787948, Length=331, Percent_Identity=31.7220543806647, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1790194, Length=331, Percent_Identity=29.9093655589124, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788474, Length=310, Percent_Identity=27.0967741935484, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1787580, Length=319, Percent_Identity=29.153605015674, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1789202, Length=300, Percent_Identity=28.3333333333333, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1789068, Length=313, Percent_Identity=27.7955271565495, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994940, Length=311, Percent_Identity=26.0450160771704, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787906, Length=335, Percent_Identity=23.8805970149254, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=23.1511254019293, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1790689, Length=317, Percent_Identity=24.2902208201893, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786540, Length=333, Percent_Identity=24.024024024024, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1786268, Length=296, Percent_Identity=22.972972972973, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NTDR_BACSU (O07567)

Other databases:

- EMBL:   Y14081
- EMBL:   AL009126
- PIR:   D69834
- RefSeq:   NP_388937.1
- ProteinModelPortal:   O07567
- SMR:   O07567
- EnsemblBacteria:   EBBACT00000003618
- GeneID:   936344
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10560
- NMPDR:   fig|224308.1.peg.1056
- GenoList:   BSU10560
- GeneTree:   EBGT00070000031749
- HOGENOM:   HBG753640
- OMA:   HSADITI
- PhylomeDB:   O07567
- ProtClustDB:   CLSK887021
- BioCyc:   BSUB:BSU10560-MONOMER
- GO:   GO:0005622
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 37687; Mature: 37556

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MPTIDEIAKLCNVSKTTVSRVLNNHPYVSKEKRDMILKAINELDYTPNYLARNFRRNKTQ
CCCHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCE
TIALSVPSIDHPFFAQLIKGVSHEVLFKNYKVIVFQTFYDKQTELELLELLKHKEVDGII
EEEEECCCCCCHHHHHHHHCCCHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEE
LGTLENEWDQISPFLKYGPILLCNEYHHSADITIIGYDEFEAAYMGVVHLIERGHKKIGF
EECCCCCHHHHCHHHHHCCEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEE
CFDTPYSEAQCQRKEGYLKALQDYNLHHRSEWIFGEMFNIEDGFRVFHKIKDLKDRPSAI
EECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCCHHHHHHHHHHHHCCCCCEE
FTGNDQVAAGIIKQAMKNGFKVPEDLAVIGFDNQLICQVVTPTITTIDIPVIELGQQAVL
EECCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEECCCEEEEECHHHHCCHHHHH
KIIESISGNASLNRRIIKLPTKLIIREST
HHHHHCCCCCHHCCCCEECCCEEEEECCC
>Mature Secondary Structure 
PTIDEIAKLCNVSKTTVSRVLNNHPYVSKEKRDMILKAINELDYTPNYLARNFRRNKTQ
CCHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCE
TIALSVPSIDHPFFAQLIKGVSHEVLFKNYKVIVFQTFYDKQTELELLELLKHKEVDGII
EEEEECCCCCCHHHHHHHHCCCHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEE
LGTLENEWDQISPFLKYGPILLCNEYHHSADITIIGYDEFEAAYMGVVHLIERGHKKIGF
EECCCCCHHHHCHHHHHCCEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEE
CFDTPYSEAQCQRKEGYLKALQDYNLHHRSEWIFGEMFNIEDGFRVFHKIKDLKDRPSAI
EECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCCHHHHHHHHHHHHCCCCCEE
FTGNDQVAAGIIKQAMKNGFKVPEDLAVIGFDNQLICQVVTPTITTIDIPVIELGQQAVL
EECCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEECCCEEEEECHHHHCCHHHHH
KIIESISGNASLNRRIIKLPTKLIIREST
HHHHHCCCCCHHCCCCEECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377