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The map label for this gene is acoL

Identifier: 16077876

GI number: 16077876

Start: 882266

End: 883642

Strand: Direct

Name: acoL

Synonym: BSU08090

Alternate gene names: 16077876

Gene position: 882266-883642 (Clockwise)

Preceding gene: 16077875

Following gene: 16077877

Centisome position: 20.93

GC content: 51.42

Gene sequence:

>1377_bases
ATGACATTAGCCATTATCGGCGGCGGACCTGCAGGCTATGCGGCTGCGGTTTCCGCGGCACAGCAGGGCAGAAACGTGCT
GCTCATTGACAAAGGCAAGCTTGGGGGGACCTGCCTGAATGAAGGCTGCATCCCGACAAAGTCTTTGTTAGAAAGCGCAA
ACGTTCTTGATAAAATCAAGCATGCCGACAGCTTTGGAATCGAACTTCCGGCAGGTGCGATATCAGTCGATTGGAGTAAA
ATGCAAAGCCGAAAACAACAGGTTGTCAGTCAGCTTGTCCAAGGCGTTCAGTACCTAATGAAGAAAAATCAAATACAGGT
TGTAAAGGGAACAGCCTCCTTTCTTTCTGAAAGAAAGCTCTTGATCGAAGGAGAAAACGGAAAAGAAATCAGAGAGGCGG
ACCAAGTATTGATTGCCTCCGGGTCAGAGCCAATCGAGCTGCCTTTTGCCCCATTTGACGGCGAATGGATCCTCGACAGC
AAAGACGCGCTTTCTCTTTCCGAGATTCCGTCTTCACTAGTCATTGTCGGCGGCGGTGTCATCGGGTGTGAGTATGCAGG
GCTGTTCGCCAGATTGGGATCGCAGGTGACCATCATTGAAACAGCGGACCGGCTGATCCCGGCTGAAGATGAAGATATTG
CCCGTCTCTTTCAGGAGAAACTTGAGGAAGACGGTGTCGAAGTGCATACTTCATCCAGATTAGGGCGGGTGGATCAAACG
GCCAAAACGGCAATATGGAAAAGCGGTCAGCGAGAGTTTAAAACGAAGGCCGATTATGTGCTGGTGGCGATCGGCAGAAA
ACCCCGTCTTGACGGATTGCAGCTGGAACAGGCCGGAGTTGATTTTTCTCCAAAGGGCATTCCGGTGAATGGGCACATGC
AGACGAACGTGCCTCATATTTACGCGTGCGGAGATGCTATAGGGGGCATTCAGCTCGCGCATGCCGCTTTCCATGAGGGC
ATCATCGCTGCTTCTCATGCTTCCGGAAGGGATGTCAAAATCAATGAGAAACATGTGCCGCGCTGCATCTATACGTCCCC
GGAAATCGCGTGTATCGGAATGACAGAACGACAGGCAAGAAGCATATACGGGGATGTGAAGATCGGCGAATTTTCATTTT
CCGCAAACGGCAAGGCGCTCATTAAACAGCAAGCGGAAGGAAAGGTCAAAATCATGGCTGAACCGGAATTCGGCGAAATC
GTGGGTGTCTCGATGATTGGCCCGGATGTAACCGAGCTCATCGGCCAAGCGGCAGCGATCATGAATGGTGAGATGACGGC
AGATATGGCGGAGCATTTTATCGCCGCCCATCCGACTTTATCGGAAACATTGCATGAGGCGCTGTTAAGCACGATCGGCC
TTGCGGTACATGCATAA

Upstream 100 bases:

>100_bases
CGTGTGACGGCGCCCCTGCCGCTGCATTCCTGAAGGCGATGAAAACATATTTGGAAGAACCCGCAGCATTAATTTTATAG
GAAAAGCAGGTGAAAACGAC

Downstream 100 bases:

>100_bases
TAAAGGAAAAAGCAGGCGCATGGATATAAGGCGCCTGCTTTTTTATTGTTGAAAGCGCTTTATTTTTCCCCTACAATAGA
TGAAAACGGCGTGTAAGGGA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of acetoin cleaving system

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTLAIIGGGPAGYAAAVSAAQQGRNVLLIDKGKLGGTCLNEGCIPTKSLLESANVLDKIKHADSFGIELPAGAISVDWSK
MQSRKQQVVSQLVQGVQYLMKKNQIQVVKGTASFLSERKLLIEGENGKEIREADQVLIASGSEPIELPFAPFDGEWILDS
KDALSLSEIPSSLVIVGGGVIGCEYAGLFARLGSQVTIIETADRLIPAEDEDIARLFQEKLEEDGVEVHTSSRLGRVDQT
AKTAIWKSGQREFKTKADYVLVAIGRKPRLDGLQLEQAGVDFSPKGIPVNGHMQTNVPHIYACGDAIGGIQLAHAAFHEG
IIAASHASGRDVKINEKHVPRCIYTSPEIACIGMTERQARSIYGDVKIGEFSFSANGKALIKQQAEGKVKIMAEPEFGEI
VGVSMIGPDVTELIGQAAAIMNGEMTADMAEHFIAAHPTLSETLHEALLSTIGLAVHA

Sequences:

>Translated_458_residues
MTLAIIGGGPAGYAAAVSAAQQGRNVLLIDKGKLGGTCLNEGCIPTKSLLESANVLDKIKHADSFGIELPAGAISVDWSK
MQSRKQQVVSQLVQGVQYLMKKNQIQVVKGTASFLSERKLLIEGENGKEIREADQVLIASGSEPIELPFAPFDGEWILDS
KDALSLSEIPSSLVIVGGGVIGCEYAGLFARLGSQVTIIETADRLIPAEDEDIARLFQEKLEEDGVEVHTSSRLGRVDQT
AKTAIWKSGQREFKTKADYVLVAIGRKPRLDGLQLEQAGVDFSPKGIPVNGHMQTNVPHIYACGDAIGGIQLAHAAFHEG
IIAASHASGRDVKINEKHVPRCIYTSPEIACIGMTERQARSIYGDVKIGEFSFSANGKALIKQQAEGKVKIMAEPEFGEI
VGVSMIGPDVTELIGQAAAIMNGEMTADMAEHFIAAHPTLSETLHEALLSTIGLAVHA
>Mature_457_residues
TLAIIGGGPAGYAAAVSAAQQGRNVLLIDKGKLGGTCLNEGCIPTKSLLESANVLDKIKHADSFGIELPAGAISVDWSKM
QSRKQQVVSQLVQGVQYLMKKNQIQVVKGTASFLSERKLLIEGENGKEIREADQVLIASGSEPIELPFAPFDGEWILDSK
DALSLSEIPSSLVIVGGGVIGCEYAGLFARLGSQVTIIETADRLIPAEDEDIARLFQEKLEEDGVEVHTSSRLGRVDQTA
KTAIWKSGQREFKTKADYVLVAIGRKPRLDGLQLEQAGVDFSPKGIPVNGHMQTNVPHIYACGDAIGGIQLAHAAFHEGI
IAASHASGRDVKINEKHVPRCIYTSPEIACIGMTERQARSIYGDVKIGEFSFSANGKALIKQQAEGKVKIMAEPEFGEIV
GVSMIGPDVTELIGQAAAIMNGEMTADMAEHFIAAHPTLSETLHEALLSTIGLAVHA

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family

Homologues:

Organism=Homo sapiens, GI91199540, Length=464, Percent_Identity=35.3448275862069, Blast_Score=265, Evalue=7e-71,
Organism=Homo sapiens, GI50301238, Length=448, Percent_Identity=31.25, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI148277065, Length=458, Percent_Identity=27.9475982532751, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI33519430, Length=458, Percent_Identity=27.9475982532751, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI33519428, Length=458, Percent_Identity=27.9475982532751, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI33519426, Length=458, Percent_Identity=27.9475982532751, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI148277071, Length=458, Percent_Identity=27.9475982532751, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI22035672, Length=458, Percent_Identity=29.4759825327511, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI291045266, Length=456, Percent_Identity=26.3157894736842, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI291045268, Length=451, Percent_Identity=25.0554323725055, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1786307, Length=447, Percent_Identity=35.7941834451902, Blast_Score=281, Evalue=9e-77,
Organism=Escherichia coli, GI87082354, Length=463, Percent_Identity=30.8855291576674, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI87081717, Length=449, Percent_Identity=28.9532293986637, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1789915, Length=435, Percent_Identity=28.5057471264368, Blast_Score=169, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI32565766, Length=458, Percent_Identity=36.0262008733624, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17557007, Length=469, Percent_Identity=28.1449893390192, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI71983419, Length=442, Percent_Identity=26.6968325791855, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71983429, Length=442, Percent_Identity=26.6968325791855, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=461, Percent_Identity=24.295010845987, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=472, Percent_Identity=36.0169491525424, Blast_Score=264, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6325166, Length=446, Percent_Identity=28.9237668161435, Blast_Score=186, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6325240, Length=468, Percent_Identity=30.7692307692308, Blast_Score=173, Evalue=6e-44,
Organism=Drosophila melanogaster, GI21358499, Length=455, Percent_Identity=36.4835164835165, Blast_Score=271, Evalue=8e-73,
Organism=Drosophila melanogaster, GI24640553, Length=461, Percent_Identity=28.8503253796095, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24640549, Length=461, Percent_Identity=28.8503253796095, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24640551, Length=461, Percent_Identity=28.8503253796095, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI17737741, Length=463, Percent_Identity=27.645788336933, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): DLDH3_BACSU (O34324)

Other databases:

- EMBL:   AF006075
- EMBL:   D78509
- EMBL:   AL009126
- PIR:   G69581
- RefSeq:   NP_388690.1
- ProteinModelPortal:   O34324
- SMR:   O34324
- EnsemblBacteria:   EBBACT00000003209
- GeneID:   939702
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU08090
- NMPDR:   fig|224308.1.peg.809
- GenoList:   BSU08090
- GeneTree:   EBGT00070000031801
- HOGENOM:   HBG515043
- OMA:   EHMQTNV
- PhylomeDB:   O34324
- ProtClustDB:   PRK06912
- BioCyc:   BSUB:BSU08090-MONOMER
- BRENDA:   1.8.1.4
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327
- Gene3D:   G3DSA:3.30.390.30
- PANTHER:   PTHR22912:SF20
- PRINTS:   PR00368
- TIGRFAMs:   TIGR01350

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim; SSF55424 FAD/NAD-linked_reductase_dimer

EC number: =1.8.1.4

Molecular weight: Translated: 48854; Mature: 48722

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: ACT_SITE 437-437 BINDING 47-47 BINDING 112-112 BINDING 200-200 BINDING 305-305 BINDING 313-313

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAIIGGGPAGYAAAVSAAQQGRNVLLIDKGKLGGTCLNEGCIPTKSLLESANVLDKIK
CEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
HADSFGIELPAGAISVDWSKMQSRKQQVVSQLVQGVQYLMKKNQIQVVKGTASFLSERKL
CCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCEE
LIEGENGKEIREADQVLIASGSEPIELPFAPFDGEWILDSKDALSLSEIPSSLVIVGGGV
EEECCCCCHHHHHCEEEEECCCCCEEECCCCCCCCEEECCCCCCCHHHCCCEEEEEECCE
IGCEYAGLFARLGSQVTIIETADRLIPAEDEDIARLFQEKLEEDGVEVHTSSRLGRVDQT
EEHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHH
AKTAIWKSGQREFKTKADYVLVAIGRKPRLDGLQLEQAGVDFSPKGIPVNGHMQTNVPHI
HHHHHHHCCCHHHCCCCCEEEEEECCCCCCCCEEEHHCCCCCCCCCCCCCCCEECCCCEE
YACGDAIGGIQLAHAAFHEGIIAASHASGRDVKINEKHVPRCIYTSPEIACIGMTERQAR
EECCCCCCCHHHHHHHHHCCEEEECCCCCCCEEECCCCCCEEEECCCCEEEEECCHHHHH
SIYGDVKIGEFSFSANGKALIKQQAEGKVKIMAEPEFGEIVGVSMIGPDVTELIGQAAAI
HHHCCEEEEEEEECCCCHHEEEECCCCCEEEEECCCCCCEEEEEECCCCHHHHHHHHHHH
MNGEMTADMAEHFIAAHPTLSETLHEALLSTIGLAVHA
HCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLAIIGGGPAGYAAAVSAAQQGRNVLLIDKGKLGGTCLNEGCIPTKSLLESANVLDKIK
EEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
HADSFGIELPAGAISVDWSKMQSRKQQVVSQLVQGVQYLMKKNQIQVVKGTASFLSERKL
CCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCEE
LIEGENGKEIREADQVLIASGSEPIELPFAPFDGEWILDSKDALSLSEIPSSLVIVGGGV
EEECCCCCHHHHHCEEEEECCCCCEEECCCCCCCCEEECCCCCCCHHHCCCEEEEEECCE
IGCEYAGLFARLGSQVTIIETADRLIPAEDEDIARLFQEKLEEDGVEVHTSSRLGRVDQT
EEHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHH
AKTAIWKSGQREFKTKADYVLVAIGRKPRLDGLQLEQAGVDFSPKGIPVNGHMQTNVPHI
HHHHHHHCCCHHHCCCCCEEEEEECCCCCCCCEEEHHCCCCCCCCCCCCCCCEECCCCEE
YACGDAIGGIQLAHAAFHEGIIAASHASGRDVKINEKHVPRCIYTSPEIACIGMTERQAR
EECCCCCCCHHHHHHHHHCCEEEECCCCCCCEEECCCCCCEEEECCCCEEEEECCHHHHH
SIYGDVKIGEFSFSANGKALIKQQAEGKVKIMAEPEFGEIVGVSMIGPDVTELIGQAAAI
HHHCCEEEEEEEECCCCHHEEEECCCCCEEEEECCCCCCEEEEEECCCCHHHHHHHHHHH
MNGEMTADMAEHFIAAHPTLSETLHEALLSTIGLAVHA
HCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10368162; 8969503; 9384377