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The map label for this gene is gabR

Identifier: 16077457

GI number: 16077457

Start: 440025

End: 441464

Strand: Reverse

Name: gabR

Synonym: BSU03890

Alternate gene names: 16077457

Gene position: 441464-440025 (Counterclockwise)

Preceding gene: 16077462

Following gene: 16077455

Centisome position: 10.47

GC content: 48.96

Gene sequence:

>1440_bases
ATGGATATCACGATTACACTCGATCGTTCAGAACAAGCCGATTATATCTATCAGCAAATTTATCAAAAGCTGAAAAAAGA
AATCCTCAGCCGCAATCTGCTGCCGCACTCGAAGGTTCCCTCCAAGCGGGAGCTGGCTGAAAATCTCAAGGTCAGCGTAA
ATTCAGTGAATTCAGCCTATCAGCAGCTGCTGGCTGAGGGGTATTTGTACGCCATTGAACGAAAGGGTTTCTTCGTGGAG
GAACTAGACATGTTTTCCGCCGAGGAGCACCCTCCATTTGCACTGCCGGATGACCTAAAAGAGATTCACATCGACCAGAG
CGATTGGATATCGTTTTCACACATGAGTTCCGATACAGACCATTTTCCGATCAAAAGCTGGTTCCGCTGCGAGCAAAAAG
CGGCCTCCCGCTCATACCGCACGCTCGGCGATATGTCACATCCGCAAGGGATATATGAAGTGAGAGCGGCCATTACGAGG
CTCATTTCCCTGACGAGGGGTGTAAAATGCAGGCCGGAACAAATGATCATAGGGGCAGGCACACAGGTGCTCATGCAGCT
GTTGACTGAGCTTTTACCCAAGGAAGCCGTGTATGCGATGGAGGAGCCTGGCTACAGGCGCATGTATCAGCTTTTGAAGA
ATGCCGGAAAACAAGTAAAGACGATCATGCTGGATGAAAAAGGCATGTCGATTGCTGAAATCACCAGACAGCAGCCAGAT
GTGCTGGTGACCACCCCGTCGCATCAGTTTCCGTCCGGAACGATTATGCCTGTATCCAGAAGAATTCAGCTGCTGAACTG
GGCAGCCGAGGAGCCGCGCCGATATATCATTGAGGACGATTATGATAGTGAATTCACATATGATGTAGACAGTATTCCGG
CGCTGCAAAGCCTCGACCGTTTTCAAAATGTCATCTATATGGGAACCTTTTCAAAGTCCCTTCTCCCCGGCTTACGGATC
AGCTATATGGTGTTGCCGCCTGAGCTGTTGAGGGCATACAAACAGCGGGGCTATGATCTGCAGACTTGCTCATCACTCAC
ACAGCTCACCCTGCAGGAATTTATCGAGTCTGGTGAATATCAGAAGCATATAAAAAAAATGAAGCAGCATTATAAAGAAA
AGAGAGAACGCCTGATCACCGCTTTAGAAGCAGAGTTCAGCGGAGAGGTTACCGTAAAAGGGGCAAATGCGGGGCTGCAT
TTTGTTACCGAATTTGATACCAGGCGCACCGAACAAGACATCCTGTCACATGCTGCCGGGCTGCAGCTTGAAATATTCGG
AATGAGCCGATTTAACTTGAAGGAAAACAAGCGGCAAACGGGCAGGCCTGCTCTCATTATCGGCTTTGCACGGCTGAAGG
AAGAAGATATTCAGGAGGGTGTGCAGCGGCTTTTCAAAGCGGTTTACGGACATAAAAAAATCCCCGTTACAGGGGATTGA

Upstream 100 bases:

>100_bases
ATCCCCCTGTCGGTATTTTCTTATCATTCTGACTTCTCTTTGGTATGATGAAAAGTACCAATTATAACTTTTTGATGGTA
TCAGAAGGAGAAACTACAAA

Downstream 100 bases:

>100_bases
TTCACCAGCGGTCCTGGTCTACATTCAGCACGGCTTCAAGCAGTCCCGGAAATGCCTTTTCAAGGTCTTCAGCGCGAAGC
GAATAATACCGGCGCTTTCC

Product: GntR/MocR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MDITITLDRSEQADYIYQQIYQKLKKEILSRNLLPHSKVPSKRELAENLKVSVNSVNSAYQQLLAEGYLYAIERKGFFVE
ELDMFSAEEHPPFALPDDLKEIHIDQSDWISFSHMSSDTDHFPIKSWFRCEQKAASRSYRTLGDMSHPQGIYEVRAAITR
LISLTRGVKCRPEQMIIGAGTQVLMQLLTELLPKEAVYAMEEPGYRRMYQLLKNAGKQVKTIMLDEKGMSIAEITRQQPD
VLVTTPSHQFPSGTIMPVSRRIQLLNWAAEEPRRYIIEDDYDSEFTYDVDSIPALQSLDRFQNVIYMGTFSKSLLPGLRI
SYMVLPPELLRAYKQRGYDLQTCSSLTQLTLQEFIESGEYQKHIKKMKQHYKEKRERLITALEAEFSGEVTVKGANAGLH
FVTEFDTRRTEQDILSHAAGLQLEIFGMSRFNLKENKRQTGRPALIIGFARLKEEDIQEGVQRLFKAVYGHKKIPVTGD

Sequences:

>Translated_479_residues
MDITITLDRSEQADYIYQQIYQKLKKEILSRNLLPHSKVPSKRELAENLKVSVNSVNSAYQQLLAEGYLYAIERKGFFVE
ELDMFSAEEHPPFALPDDLKEIHIDQSDWISFSHMSSDTDHFPIKSWFRCEQKAASRSYRTLGDMSHPQGIYEVRAAITR
LISLTRGVKCRPEQMIIGAGTQVLMQLLTELLPKEAVYAMEEPGYRRMYQLLKNAGKQVKTIMLDEKGMSIAEITRQQPD
VLVTTPSHQFPSGTIMPVSRRIQLLNWAAEEPRRYIIEDDYDSEFTYDVDSIPALQSLDRFQNVIYMGTFSKSLLPGLRI
SYMVLPPELLRAYKQRGYDLQTCSSLTQLTLQEFIESGEYQKHIKKMKQHYKEKRERLITALEAEFSGEVTVKGANAGLH
FVTEFDTRRTEQDILSHAAGLQLEIFGMSRFNLKENKRQTGRPALIIGFARLKEEDIQEGVQRLFKAVYGHKKIPVTGD
>Mature_479_residues
MDITITLDRSEQADYIYQQIYQKLKKEILSRNLLPHSKVPSKRELAENLKVSVNSVNSAYQQLLAEGYLYAIERKGFFVE
ELDMFSAEEHPPFALPDDLKEIHIDQSDWISFSHMSSDTDHFPIKSWFRCEQKAASRSYRTLGDMSHPQGIYEVRAAITR
LISLTRGVKCRPEQMIIGAGTQVLMQLLTELLPKEAVYAMEEPGYRRMYQLLKNAGKQVKTIMLDEKGMSIAEITRQQPD
VLVTTPSHQFPSGTIMPVSRRIQLLNWAAEEPRRYIIEDDYDSEFTYDVDSIPALQSLDRFQNVIYMGTFSKSLLPGLRI
SYMVLPPELLRAYKQRGYDLQTCSSLTQLTLQEFIESGEYQKHIKKMKQHYKEKRERLITALEAEFSGEVTVKGANAGLH
FVTEFDTRRTEQDILSHAAGLQLEIFGMSRFNLKENKRQTGRPALIIGFARLKEEDIQEGVQRLFKAVYGHKKIPVTGD

Specific function: Activates the transcription of the gabTD operon. Is also a repressor of its own expression, both in the presence and absence of GABA. Binds specifically to the DNA region overlapping the -35 region of the gabT promoter and the -10 and +1 regions of the ga

COG id: COG1167

COG function: function code KE; Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1790797, Length=474, Percent_Identity=24.4725738396624, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787710, Length=411, Percent_Identity=23.8442822384428, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): GABR_BACSU (P94426)

Other databases:

- EMBL:   D50453
- EMBL:   AL009126
- PIR:   B69764
- RefSeq:   NP_388271.1
- ProteinModelPortal:   P94426
- EnsemblBacteria:   EBBACT00000001607
- GeneID:   938271
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU03890
- NMPDR:   fig|224308.1.peg.390
- GenoList:   BSU03890
- GeneTree:   EBGT00070000031960
- HOGENOM:   HBG752297
- OMA:   WISFSHM
- PhylomeDB:   P94426
- ProtClustDB:   CLSK886706
- BioCyc:   BSUB:BSU03890-MONOMER
- GO:   GO:0005622
- InterPro:   IPR004839
- InterPro:   IPR000524
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR011991
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:1.10.10.10
- SMART:   SM00345

Pfam domain/function: PF00155 Aminotran_1_2; PF00392 GntR; SSF53383 PyrdxlP-dep_Trfase_major

EC number: NA

Molecular weight: Translated: 55167; Mature: 55167

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITITLDRSEQADYIYQQIYQKLKKEILSRNLLPHSKVPSKRELAENLKVSVNSVNSAY
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QQLLAEGYLYAIERKGFFVEELDMFSAEEHPPFALPDDLKEIHIDQSDWISFSHMSSDTD
HHHHHCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCHHHHHCCCHHHCEEECCCCCCCC
HFPIKSWFRCEQKAASRSYRTLGDMSHPQGIYEVRAAITRLISLTRGVKCRPEQMIIGAG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCC
TQVLMQLLTELLPKEAVYAMEEPGYRRMYQLLKNAGKQVKTIMLDEKGMSIAEITRQQPD
HHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCC
VLVTTPSHQFPSGTIMPVSRRIQLLNWAAEEPRRYIIEDDYDSEFTYDVDSIPALQSLDR
EEEECCCCCCCCCCEEEHHHHHHHHHHHHCCCCEEEECCCCCCCEEECCCCCHHHHHHHH
FQNVIYMGTFSKSLLPGLRISYMVLPPELLRAYKQRGYDLQTCSSLTQLTLQEFIESGEY
HHHEEEEECCHHHHCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
QKHIKKMKQHYKEKRERLITALEAEFSGEVTVKGANAGLHFVTEFDTRRTEQDILSHAAG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHCC
LQLEIFGMSRFNLKENKRQTGRPALIIGFARLKEEDIQEGVQRLFKAVYGHKKIPVTGD
CEEEEECCCCCCCCHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDITITLDRSEQADYIYQQIYQKLKKEILSRNLLPHSKVPSKRELAENLKVSVNSVNSAY
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QQLLAEGYLYAIERKGFFVEELDMFSAEEHPPFALPDDLKEIHIDQSDWISFSHMSSDTD
HHHHHCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCHHHHHCCCHHHCEEECCCCCCCC
HFPIKSWFRCEQKAASRSYRTLGDMSHPQGIYEVRAAITRLISLTRGVKCRPEQMIIGAG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCC
TQVLMQLLTELLPKEAVYAMEEPGYRRMYQLLKNAGKQVKTIMLDEKGMSIAEITRQQPD
HHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCC
VLVTTPSHQFPSGTIMPVSRRIQLLNWAAEEPRRYIIEDDYDSEFTYDVDSIPALQSLDR
EEEECCCCCCCCCCEEEHHHHHHHHHHHHCCCCEEEECCCCCCCEEECCCCCHHHHHHHH
FQNVIYMGTFSKSLLPGLRISYMVLPPELLRAYKQRGYDLQTCSSLTQLTLQEFIESGEY
HHHEEEEECCHHHHCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
QKHIKKMKQHYKEKRERLITALEAEFSGEVTVKGANAGLHFVTEFDTRRTEQDILSHAAG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHCC
LQLEIFGMSRFNLKENKRQTGRPALIIGFARLKEEDIQEGVQRLFKAVYGHKKIPVTGD
CEEEEECCCCCCCCHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377