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The map label for this gene is carA [H]

Identifier: 159900755

GI number: 159900755

Start: 5410258

End: 5411388

Strand: Direct

Name: carA [H]

Synonym: Haur_4242

Alternate gene names: 159900755

Gene position: 5410258-5411388 (Clockwise)

Preceding gene: 159900750

Following gene: 159900756

Centisome position: 85.25

GC content: 53.05

Gene sequence:

>1131_bases
ATGACACGCGCATTACTGGCATTGGAAGATGGACGCACCTTTTGGGGGCGGGCTGTCGGTGCACGCGGCGAACGTGCAGG
TGAAGTTGTATTCAATACCAGCATGACTGGCTACGCTGAAATTTTGACCGATCCTTCATATCGCGGTCAGTTAGTAACCT
TAACCGCCTCGCATATTGGCAATTATGGCATTGACGAGGTTGATCTTGAGGCAGCCATGCCTTGGGCCGAAGCCTTGATC
GTGCGTTCATTTACCGAGCGGCCATCCAATTGGCGTTCGCGCGAATCTCTCAGCGAGCTATTAGCACGGCGCGGTGTTAT
GGCGGTGGCTGATTTAGATACCCGAGCCTTGACTCGCCATATTCGGGCAGCCGGCGCAATGCGGGCGGTGCTCTCAACCG
AAGATCTTGATCCGGCTAGTTTGGTCGCCAAAGCTCAAGCCATCCCGGTGATGGAAGGCCGCGATTTGGCTAGCGACGTG
GGAACCCAAAGCATTTATGAGTGGAATGAAGGCACACCTGCCGATTTCACTACCTTACAACTAGCGATTCCTGAACAATT
GCACAACCGCCATGTCGTAGTTTACGATTTTGGGGTCAAACGCAACACCCTGCGGCGCTTGGTCGATCTTGGTTGTAAGG
TCACAGTTGTGCCCAATCGCACTTCAGCCGAAGCGACCCTAGCCTTAAAACCTGATGGTATTTTGATTTCAAATGGCCCA
GGCGACCCTGCCACCTTGGAGTATGCGGTCGAAACGATTCGCCAGTTGATCGGCAATGTGCCAGTGTTTGGCATCTGCCT
TGGGCATCAATTGATTGGCCAAGCCTTGGGTGGTACAACCTTCAAATTGCCTTTTGGTCATCATGCTGGCAATCACCCAG
TGTGCGATACCAGCACTGGCAAAGTCCGAATCACTGCTCAAAATCATGGCTTTGCCCTCGATCCAGCCAGCTTGCCCAGC
GATGTGCAGGTGACCGAAGTTAGTGGTAACGACCAAACCTGTGAAGGCTTGCAACACAAGAGCTTGCCTGTTTTCAGCGT
GCAATATCACCCTGAGGCTGGGCCTGGCCCTCACGATGGAGATGAACACTTCCGGCGTTTTATCAGCCTCGTTGATCAAC
AACGTAGCTAA

Upstream 100 bases:

>100_bases
AACGCAAGCAGCAACCCCAAAAAATGCTGATTCCGTTATAATACTCGGTTGGTACTCAGTTTTGGCTGAACCACCCGATT
GCTTCTAGCTTGGAGTTGCC

Downstream 100 bases:

>100_bases
ATTTCCAAACCGATTGCCCCAAAACCTCGATAATCGGCAAAATTATCGAGGTTTTCTATTTAATCCCATTTAAAACTGAA
CTTTTTAACGTTACCCTCAG

Product: carbamoyl-phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTRALLALEDGRTFWGRAVGARGERAGEVVFNTSMTGYAEILTDPSYRGQLVTLTASHIGNYGIDEVDLEAAMPWAEALI
VRSFTERPSNWRSRESLSELLARRGVMAVADLDTRALTRHIRAAGAMRAVLSTEDLDPASLVAKAQAIPVMEGRDLASDV
GTQSIYEWNEGTPADFTTLQLAIPEQLHNRHVVVYDFGVKRNTLRRLVDLGCKVTVVPNRTSAEATLALKPDGILISNGP
GDPATLEYAVETIRQLIGNVPVFGICLGHQLIGQALGGTTFKLPFGHHAGNHPVCDTSTGKVRITAQNHGFALDPASLPS
DVQVTEVSGNDQTCEGLQHKSLPVFSVQYHPEAGPGPHDGDEHFRRFISLVDQQRS

Sequences:

>Translated_376_residues
MTRALLALEDGRTFWGRAVGARGERAGEVVFNTSMTGYAEILTDPSYRGQLVTLTASHIGNYGIDEVDLEAAMPWAEALI
VRSFTERPSNWRSRESLSELLARRGVMAVADLDTRALTRHIRAAGAMRAVLSTEDLDPASLVAKAQAIPVMEGRDLASDV
GTQSIYEWNEGTPADFTTLQLAIPEQLHNRHVVVYDFGVKRNTLRRLVDLGCKVTVVPNRTSAEATLALKPDGILISNGP
GDPATLEYAVETIRQLIGNVPVFGICLGHQLIGQALGGTTFKLPFGHHAGNHPVCDTSTGKVRITAQNHGFALDPASLPS
DVQVTEVSGNDQTCEGLQHKSLPVFSVQYHPEAGPGPHDGDEHFRRFISLVDQQRS
>Mature_375_residues
TRALLALEDGRTFWGRAVGARGERAGEVVFNTSMTGYAEILTDPSYRGQLVTLTASHIGNYGIDEVDLEAAMPWAEALIV
RSFTERPSNWRSRESLSELLARRGVMAVADLDTRALTRHIRAAGAMRAVLSTEDLDPASLVAKAQAIPVMEGRDLASDVG
TQSIYEWNEGTPADFTTLQLAIPEQLHNRHVVVYDFGVKRNTLRRLVDLGCKVTVVPNRTSAEATLALKPDGILISNGPG
DPATLEYAVETIRQLIGNVPVFGICLGHQLIGQALGGTTFKLPFGHHAGNHPVCDTSTGKVRITAQNHGFALDPASLPSD
VQVTEVSGNDQTCEGLQHKSLPVFSVQYHPEAGPGPHDGDEHFRRFISLVDQQRS

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=384, Percent_Identity=36.71875, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI21361331, Length=387, Percent_Identity=34.1085271317829, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI169790915, Length=387, Percent_Identity=34.1085271317829, Blast_Score=210, Evalue=2e-54,
Organism=Escherichia coli, GI1786215, Length=382, Percent_Identity=50.261780104712, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1789760, Length=159, Percent_Identity=31.4465408805031, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=388, Percent_Identity=37.8865979381443, Blast_Score=238, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6324878, Length=390, Percent_Identity=35.8974358974359, Blast_Score=239, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6322331, Length=394, Percent_Identity=36.2944162436548, Blast_Score=232, Evalue=9e-62,
Organism=Saccharomyces cerevisiae, GI6322638, Length=175, Percent_Identity=33.7142857142857, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI45555749, Length=393, Percent_Identity=35.1145038167939, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24642586, Length=393, Percent_Identity=35.1145038167939, Blast_Score=219, Evalue=2e-57,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40761; Mature: 40630

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRALLALEDGRTFWGRAVGARGERAGEVVFNTSMTGYAEILTDPSYRGQLVTLTASHIG
CCCEEEEEECCCCHHHHHHCCCCCCCCEEEEECCCCCHHHHHCCCCCCCEEEEEEHHHCC
NYGIDEVDLEAAMPWAEALIVRSFTERPSNWRSRESLSELLARRGVMAVADLDTRALTRH
CCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHH
IRAAGAMRAVLSTEDLDPASLVAKAQAIPVMEGRDLASDVGTQSIYEWNEGTPADFTTLQ
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHCCCCEEECCCCCCCCEEEEE
LAIPEQLHNRHVVVYDFGVKRNTLRRLVDLGCKVTVVPNRTSAEATLALKPDGILISNGP
EECCHHHCCCEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCEEEECCC
GDPATLEYAVETIRQLIGNVPVFGICLGHQLIGQALGGTTFKLPFGHHAGNHPVCDTSTG
CCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCC
KVRITAQNHGFALDPASLPSDVQVTEVSGNDQTCEGLQHKSLPVFSVQYHPEAGPGPHDG
EEEEEECCCCEEECCCCCCCCCEEEEECCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCC
DEHFRRFISLVDQQRS
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRALLALEDGRTFWGRAVGARGERAGEVVFNTSMTGYAEILTDPSYRGQLVTLTASHIG
CCEEEEEECCCCHHHHHHCCCCCCCCEEEEECCCCCHHHHHCCCCCCCEEEEEEHHHCC
NYGIDEVDLEAAMPWAEALIVRSFTERPSNWRSRESLSELLARRGVMAVADLDTRALTRH
CCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHH
IRAAGAMRAVLSTEDLDPASLVAKAQAIPVMEGRDLASDVGTQSIYEWNEGTPADFTTLQ
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHCCCCEEECCCCCCCCEEEEE
LAIPEQLHNRHVVVYDFGVKRNTLRRLVDLGCKVTVVPNRTSAEATLALKPDGILISNGP
EECCHHHCCCEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCEEEECCC
GDPATLEYAVETIRQLIGNVPVFGICLGHQLIGQALGGTTFKLPFGHHAGNHPVCDTSTG
CCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCC
KVRITAQNHGFALDPASLPSDVQVTEVSGNDQTCEGLQHKSLPVFSVQYHPEAGPGPHDG
EEEEEECCCCEEECCCCCCCCCEEEEECCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCC
DEHFRRFISLVDQQRS
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA