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The map label for this gene is metB [H]

Identifier: 159900529

GI number: 159900529

Start: 5122957

End: 5124075

Strand: Direct

Name: metB [H]

Synonym: Haur_4016

Alternate gene names: 159900529

Gene position: 5122957-5124075 (Clockwise)

Preceding gene: 159900528

Following gene: 159900533

Centisome position: 80.72

GC content: 55.5

Gene sequence:

>1119_bases
ATGAAACCTGAAACCCTGTTAATTCATGCTGGTCGTAGCGTCGATGCTGCGACCAGTGCAGTTACGCCGCCGATTCATCT
TGCCAGTACCTTTGAACGAGCGGCTGATGGTAGTTTGCCCCATGGGTTTGTCTATACACGTTTTGGTAACCCCACCCGCC
AAGCCTTGGAACAAGCCTTAGCGGCACTTGAAGGTGGAGTTGAGGCGGCGGCATTTGGCTCTGGTTCGGCGGCTACAACC
GCAGTGCTGCAAAGTTTAGCGCCAGGCCAGCGCGTGTTGTTGCCACGCGATTGCTACAACGGCACGGCCAATTTGGTGCG
TCAGGTATTTGCTCAACTTGATGCTCAATTTGTCGATATGACCGACTTGGCGGCAGTGCAAGCAGCGTTGGAGCCAGCCC
CAGCCTTGGTTTGGCTCGAAACGCCATCCAACCCAACCTTGCGATTAACAGATTTGGCGGCGGTGAGCAATTTGGCGCAT
GCGGTTGGGGCATTGGTGGTTTGCGATAATACCTGGGCCACGCCGCTTGGCCAGCGCCCATTTGATTTGGGCGTGGATTT
GGTGATGCATTCGACCACCAAATATCTTGGCGGCCATAGCGATGTGCTGGGTGGTGCATTAATTACCAAAACTGTAACAC
CTTGGTGGCAACGGTTGCAGCAAATTCATGTGCTGGCCGGAGCCGTGCCCTCGCCGTTTGAATGTTGGCTGATTTTGCGC
GGCATGCAAAGTTTGGCCTATCGGCTGCGCGGCCATTGTGCCAATGCTTTGGCGGTGGCTGAGTGGTTGGCACAACATCC
CAAGGTGCAGGCTGTGCATTATCCTGGCTTGACAAGCCATCCTCAATTTGAATTGGCTCAACGCCAAATGCTGCTGATGG
GCGGTATGGTTTCGTTCGAGGTAGTTGGTGGTGCGGCTGAAGCGATTGCAGTAGCGGCCCACGTTAAGTTATGGACGCGG
GCAACCAGCCTTGGTGGCCCTGAAAGTTTGATCGAGCATCGGGCCACACTCGAAGGCCCAGATTCGCCAACCCCGCCAGC
CTTGTTGCGGCTTTCGGTCGGCCTCGAACACCCCGATGATTTGATTGCCGATTTGGCCCAAGCCTTGGCAGTATTGTAG

Upstream 100 bases:

>100_bases
TTATTAGGCCGCGCCCTCTACTTCCAAGATAGTCAGCCAAACTACATTGAGCCAGTTGCCCCCACCGCAGTTCCGGTCAT
TCCTTAGCCCTGAGAAACCT

Downstream 100 bases:

>100_bases
CTACCACACCAAGCCGATCAGCAAATCATTGACATTGGTCAGGGTTGGGCCGATTTCGATGGCGCTGCCAAGCGCTCGCC
AAACATTATAACTATCGTGG

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKPETLLIHAGRSVDAATSAVTPPIHLASTFERAADGSLPHGFVYTRFGNPTRQALEQALAALEGGVEAAAFGSGSAATT
AVLQSLAPGQRVLLPRDCYNGTANLVRQVFAQLDAQFVDMTDLAAVQAALEPAPALVWLETPSNPTLRLTDLAAVSNLAH
AVGALVVCDNTWATPLGQRPFDLGVDLVMHSTTKYLGGHSDVLGGALITKTVTPWWQRLQQIHVLAGAVPSPFECWLILR
GMQSLAYRLRGHCANALAVAEWLAQHPKVQAVHYPGLTSHPQFELAQRQMLLMGGMVSFEVVGGAAEAIAVAAHVKLWTR
ATSLGGPESLIEHRATLEGPDSPTPPALLRLSVGLEHPDDLIADLAQALAVL

Sequences:

>Translated_372_residues
MKPETLLIHAGRSVDAATSAVTPPIHLASTFERAADGSLPHGFVYTRFGNPTRQALEQALAALEGGVEAAAFGSGSAATT
AVLQSLAPGQRVLLPRDCYNGTANLVRQVFAQLDAQFVDMTDLAAVQAALEPAPALVWLETPSNPTLRLTDLAAVSNLAH
AVGALVVCDNTWATPLGQRPFDLGVDLVMHSTTKYLGGHSDVLGGALITKTVTPWWQRLQQIHVLAGAVPSPFECWLILR
GMQSLAYRLRGHCANALAVAEWLAQHPKVQAVHYPGLTSHPQFELAQRQMLLMGGMVSFEVVGGAAEAIAVAAHVKLWTR
ATSLGGPESLIEHRATLEGPDSPTPPALLRLSVGLEHPDDLIADLAQALAVL
>Mature_372_residues
MKPETLLIHAGRSVDAATSAVTPPIHLASTFERAADGSLPHGFVYTRFGNPTRQALEQALAALEGGVEAAAFGSGSAATT
AVLQSLAPGQRVLLPRDCYNGTANLVRQVFAQLDAQFVDMTDLAAVQAALEPAPALVWLETPSNPTLRLTDLAAVSNLAH
AVGALVVCDNTWATPLGQRPFDLGVDLVMHSTTKYLGGHSDVLGGALITKTVTPWWQRLQQIHVLAGAVPSPFECWLILR
GMQSLAYRLRGHCANALAVAEWLAQHPKVQAVHYPGLTSHPQFELAQRQMLLMGGMVSFEVVGGAAEAIAVAAHVKLWTR
ATSLGGPESLIEHRATLEGPDSPTPPALLRLSVGLEHPDDLIADLAQALAVL

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=362, Percent_Identity=41.7127071823204, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI299473759, Length=362, Percent_Identity=38.6740331491713, Blast_Score=225, Evalue=6e-59,
Organism=Homo sapiens, GI25453487, Length=361, Percent_Identity=37.1191135734072, Blast_Score=202, Evalue=3e-52,
Organism=Escherichia coli, GI1790375, Length=384, Percent_Identity=39.0625, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1789383, Length=390, Percent_Identity=31.025641025641, Blast_Score=165, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17538055, Length=381, Percent_Identity=41.4698162729659, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17560056, Length=378, Percent_Identity=39.6825396825397, Blast_Score=259, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI71980708, Length=372, Percent_Identity=36.5591397849462, Blast_Score=228, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6319307, Length=387, Percent_Identity=40.3100775193798, Blast_Score=254, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6321904, Length=387, Percent_Identity=29.1989664082687, Blast_Score=146, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6323333, Length=438, Percent_Identity=30.5936073059361, Blast_Score=142, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6321254, Length=404, Percent_Identity=27.4752475247525, Blast_Score=139, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6321170, Length=303, Percent_Identity=29.042904290429, Blast_Score=126, Evalue=7e-30,
Organism=Drosophila melanogaster, GI19922554, Length=377, Percent_Identity=41.3793103448276, Blast_Score=276, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 39361; Mature: 39361

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPETLLIHAGRSVDAATSAVTPPIHLASTFERAADGSLPHGFVYTRFGNPTRQALEQAL
CCCCEEEEECCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHH
AALEGGVEAAAFGSGSAATTAVLQSLAPGQRVLLPRDCYNGTANLVRQVFAQLDAQFVDM
HHHHCCCCEEECCCCCHHHHHHHHHCCCCCEEEECHHHCCCHHHHHHHHHHHHCHHHHHH
TDLAAVQAALEPAPALVWLETPSNPTLRLTDLAAVSNLAHAVGALVVCDNTWATPLGQRP
HHHHHHHHHHCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHEEEEECCCCCCCCCCCC
FDLGVDLVMHSTTKYLGGHSDVLGGALITKTVTPWWQRLQQIHVLAGAVPSPFECWLILR
HHHCHHHHHHHHHHHHCCCCHHHCCHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
GMQSLAYRLRGHCANALAVAEWLAQHPKVQAVHYPGLTSHPQFELAQRQMLLMGGMVSFE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEE
VVGGAAEAIAVAAHVKLWTRATSLGGPESLIEHRATLEGPDSPTPPALLRLSVGLEHPDD
EECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHEEEECCCCCHHH
LIADLAQALAVL
HHHHHHHHHHCC
>Mature Secondary Structure
MKPETLLIHAGRSVDAATSAVTPPIHLASTFERAADGSLPHGFVYTRFGNPTRQALEQAL
CCCCEEEEECCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHH
AALEGGVEAAAFGSGSAATTAVLQSLAPGQRVLLPRDCYNGTANLVRQVFAQLDAQFVDM
HHHHCCCCEEECCCCCHHHHHHHHHCCCCCEEEECHHHCCCHHHHHHHHHHHHCHHHHHH
TDLAAVQAALEPAPALVWLETPSNPTLRLTDLAAVSNLAHAVGALVVCDNTWATPLGQRP
HHHHHHHHHHCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHEEEEECCCCCCCCCCCC
FDLGVDLVMHSTTKYLGGHSDVLGGALITKTVTPWWQRLQQIHVLAGAVPSPFECWLILR
HHHCHHHHHHHHHHHHCCCCHHHCCHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
GMQSLAYRLRGHCANALAVAEWLAQHPKVQAVHYPGLTSHPQFELAQRQMLLMGGMVSFE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEE
VVGGAAEAIAVAAHVKLWTRATSLGGPESLIEHRATLEGPDSPTPPALLRLSVGLEHPDD
EECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHEEEECCCCCHHH
LIADLAQALAVL
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]