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The map label for this gene is bhbA [H]

Identifier: 159900500

GI number: 159900500

Start: 5086566

End: 5086979

Strand: Direct

Name: bhbA [H]

Synonym: Haur_3987

Alternate gene names: 159900500

Gene position: 5086566-5086979 (Clockwise)

Preceding gene: 159900499

Following gene: 159900501

Centisome position: 80.15

GC content: 52.17

Gene sequence:

>414_bases
ATGGAACGACCAATTCGGGTGTTAGTGGCCAAACCAGGGCTTGATGGCCACGACCGTGGCGCAAAAGTGATCGCCCGTGC
CTTGCGTGATGCAGGCATGGAAGTTATTTATACTGGGTTGCAACAAACCCCCCAAATGATCGTTGAAGCCGCCTTGCAAG
AAGACGTTGATTGCATCGGCTTGTCGATTCTCTCAGGGGCGCATATGACCTTGCTGCCCAAAATCACCACCATGCTGCGC
GAAAACGATGGCGCGGATATTTTGGTTGTAGCTGGCGGCATTATCTCCGATGAAGATGCAGCAATCTTGCGCAACGATCA
TGGGATTGCGGGGATTTTCGGGCCTGGCTCTTCGACCCATGCAATTATCGATTTTATTCGTGAGCATGTTGAGCGTAGCT
CGGTAGAAGGGTAG

Upstream 100 bases:

>100_bases
AATGAAGTGACGATCTGCAACCGATTGCTGATTCGCAGGTGGGCTAAACTGCGCTACAATGGGGCATATCGTCATTTCTC
AATGTGGAGGAAAGACCCTC

Downstream 100 bases:

>100_bases
GTTGGGCGTGGAACTTGTAGCAAAGGCTCGTGCTGGCAATCGCCGTGCACTGGCCCGCTTGATAACCTTGGTTGAAGCTG
GCGATGCCCAAGCCCAAACG

Product: cobalamin B12-binding domain-containing protein

Products: NA

Alternate protein names: MCM [H]

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MERPIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGLQQTPQMIVEAALQEDVDCIGLSILSGAHMTLLPKITTMLR
ENDGADILVVAGGIISDEDAAILRNDHGIAGIFGPGSSTHAIIDFIREHVERSSVEG

Sequences:

>Translated_137_residues
MERPIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGLQQTPQMIVEAALQEDVDCIGLSILSGAHMTLLPKITTMLR
ENDGADILVVAGGIISDEDAAILRNDHGIAGIFGPGSSTHAIIDFIREHVERSSVEG
>Mature_137_residues
MERPIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGLQQTPQMIVEAALQEDVDCIGLSILSGAHMTLLPKITTMLR
ENDGADILVVAGGIISDEDAAILRNDHGIAGIFGPGSSTHAIIDFIREHVERSSVEG

Specific function: Catalyzes the interconversion of succinyl-CoA and methylmalonyl-CoA during the polyhydroxyalkanoate degradation pathway [H]

COG id: COG2185

COG function: function code I; Methylmalonyl-CoA mutase, C-terminal domain/subunit (cobalamin-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=115, Percent_Identity=44.3478260869565, Blast_Score=92, Evalue=2e-19,
Organism=Escherichia coli, GI1789284, Length=131, Percent_Identity=42.7480916030534, Blast_Score=102, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25144500, Length=129, Percent_Identity=44.9612403100775, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 14592; Mature: 14592

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERPIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGLQQTPQMIVEAALQEDVDCIG
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LSILSGAHMTLLPKITTMLRENDGADILVVAGGIISDEDAAILRNDHGIAGIFGPGSSTH
HHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCEECCCCCEEEECCCCEEEEECCCCCHH
AIIDFIREHVERSSVEG
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MERPIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGLQQTPQMIVEAALQEDVDCIG
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LSILSGAHMTLLPKITTMLRENDGADILVVAGGIISDEDAAILRNDHGIAGIFGPGSSTH
HHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCEECCCCCEEEECCCCEEEEECCCCCHH
AIIDFIREHVERSSVEG
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9889346; 11481431 [H]