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The map label for this gene is gatA [H]

Identifier: 159900498

GI number: 159900498

Start: 5083485

End: 5084954

Strand: Direct

Name: gatA [H]

Synonym: Haur_3985

Alternate gene names: 159900498

Gene position: 5083485-5084954 (Clockwise)

Preceding gene: 159900493

Following gene: 159900499

Centisome position: 80.1

GC content: 53.67

Gene sequence:

>1470_bases
ATGCGTGAGGATGTGATTATGGCCCTCCATGAATTGACAATTGCCGAGGCCCGCGCCCAATTAGATCGCGGTGAGATTAC
CTCGGTGCAACTGACCGAGGCTCTGCTGGAACGCATCGCCGCGTTAGAGCCAACCCTGCATTCGTTTTTAACTCAAACGC
CCGATTTGGCCCTGCAACAAGCGCAAGCCGCCGACGAGCGCATCAAGGCTGGCAACGCCACGAGCTTAACTGGTATTCCC
TTGGGCATTAAAGATGTGATTGTCACCAAAGATGTGCGCACTACCTGCGGCTCGAAAGTGCTGGAAAATTGGGTTCCGCC
CTACGATGCCACCGTCGTGAGCAAACTCAACGAGGCTGGCACGGTAACCCTCGGCAAGCTTAATATGGATGAGTTTGCCA
TGGGTTCATCCAATGAAAATAGTGCGTTTGGCGGTACACGCAACCCATGGGATACCGAACGTGTGCCTGGTGGCTCGTCG
GGTGGTTCGGCGGCGGCGGTTGCTGCTGGCCAAGCTTTTGGCACATTGGGCACCGATACTGGTGGTTCAATTCGCCAACC
TGCCGCGTTGTGTGGCATCGTTGGTTTGAAGCCAACCTACGGGCGGGTTTCACGCTATGGTTTGGTAGCCTTTGCCTCAT
CACTCGATCAATGTGGGCCGTTCGCCCGCACGGTTGAAGATGTGGCCTTGTTGCTCGAAACGATTGCTGGTCATGATCCC
AAAGATTCAACCAGCGCCAATTTGCCAGTACCCGACTATCGTGCTAGCCTCACTGGCGATATTCGCGGCCTCAAAGTTGG
CGTGCCCAGCGAATATTTCGTCGATGGTATGGAGCCTGGGGTTGAAAAAGCCGTGCGCACGGCGATCGAGCAGATTAAAG
ATTTAGGTGCTGAAATTGTTGAGGTTTCGCTGCCCCACACCAAATATGCCTTGCCCGTCTATTACATCATTGCGCCAGCC
GAATGTAGCACCAACTTGGCTCGCTTCGATGGTGTGCGCTATGGCGTGCGATTAGAAGGCAATGGGCTGTTCGATGAAAT
TGAATCGACGCGTGGCCAATTGTTTGGCGCGGAAGTGCGCCGCCGGATTATGCTTGGAACGTATGCACTCTCATCGGGCT
ATTACGATGCCTACTATCGCCGCGCCCAACAAGTACGAACCTTGATTCGCCGCGACTTCCAAGAAGTTTGGAGCAAAGTC
GATCTATTGGCAGCACCAACCTCGCCCAGCGTGGCCTTCAAAATTGGCGAAAAAGCTGATGATCCAGTAGCGATGTATCT
CTCGGATGTGTGTACATTGCCGATTAACTTAGCGGGTGTGCCTGGTTTGGTTGTACCCTGTGGCTTCAGCGACAATTTGC
CAACAGGCCTGCAATTAATTGGCAAGCCATTCGATGAAGCGACCTTGCTGCGCGTCGGCCATGCCTACGAGCAAAATAAC
AGCTGGCACAAACAACATCCTAATTTGTAG

Upstream 100 bases:

>100_bases
AACCTACCCTTGAAAGTTAGGGGTGAGGAAACTTATTTATAGCTTTTGATCCTGAAACGATGCGCTATGCTACAATGCGC
GTTGGTAAACAAGCAAGCCC

Downstream 100 bases:

>100_bases
CCAAACGTTTCGTAACCACGAAGAACGCGAAGATCACTAAGTAACGATCCTTCACGCTCTTCGTGGTTAAATTTCATCCC
TCATCCTTCATCCCTCATAC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MREDVIMALHELTIAEARAQLDRGEITSVQLTEALLERIAALEPTLHSFLTQTPDLALQQAQAADERIKAGNATSLTGIP
LGIKDVIVTKDVRTTCGSKVLENWVPPYDATVVSKLNEAGTVTLGKLNMDEFAMGSSNENSAFGGTRNPWDTERVPGGSS
GGSAAAVAAGQAFGTLGTDTGGSIRQPAALCGIVGLKPTYGRVSRYGLVAFASSLDQCGPFARTVEDVALLLETIAGHDP
KDSTSANLPVPDYRASLTGDIRGLKVGVPSEYFVDGMEPGVEKAVRTAIEQIKDLGAEIVEVSLPHTKYALPVYYIIAPA
ECSTNLARFDGVRYGVRLEGNGLFDEIESTRGQLFGAEVRRRIMLGTYALSSGYYDAYYRRAQQVRTLIRRDFQEVWSKV
DLLAAPTSPSVAFKIGEKADDPVAMYLSDVCTLPINLAGVPGLVVPCGFSDNLPTGLQLIGKPFDEATLLRVGHAYEQNN
SWHKQHPNL

Sequences:

>Translated_489_residues
MREDVIMALHELTIAEARAQLDRGEITSVQLTEALLERIAALEPTLHSFLTQTPDLALQQAQAADERIKAGNATSLTGIP
LGIKDVIVTKDVRTTCGSKVLENWVPPYDATVVSKLNEAGTVTLGKLNMDEFAMGSSNENSAFGGTRNPWDTERVPGGSS
GGSAAAVAAGQAFGTLGTDTGGSIRQPAALCGIVGLKPTYGRVSRYGLVAFASSLDQCGPFARTVEDVALLLETIAGHDP
KDSTSANLPVPDYRASLTGDIRGLKVGVPSEYFVDGMEPGVEKAVRTAIEQIKDLGAEIVEVSLPHTKYALPVYYIIAPA
ECSTNLARFDGVRYGVRLEGNGLFDEIESTRGQLFGAEVRRRIMLGTYALSSGYYDAYYRRAQQVRTLIRRDFQEVWSKV
DLLAAPTSPSVAFKIGEKADDPVAMYLSDVCTLPINLAGVPGLVVPCGFSDNLPTGLQLIGKPFDEATLLRVGHAYEQNN
SWHKQHPNL
>Mature_489_residues
MREDVIMALHELTIAEARAQLDRGEITSVQLTEALLERIAALEPTLHSFLTQTPDLALQQAQAADERIKAGNATSLTGIP
LGIKDVIVTKDVRTTCGSKVLENWVPPYDATVVSKLNEAGTVTLGKLNMDEFAMGSSNENSAFGGTRNPWDTERVPGGSS
GGSAAAVAAGQAFGTLGTDTGGSIRQPAALCGIVGLKPTYGRVSRYGLVAFASSLDQCGPFARTVEDVALLLETIAGHDP
KDSTSANLPVPDYRASLTGDIRGLKVGVPSEYFVDGMEPGVEKAVRTAIEQIKDLGAEIVEVSLPHTKYALPVYYIIAPA
ECSTNLARFDGVRYGVRLEGNGLFDEIESTRGQLFGAEVRRRIMLGTYALSSGYYDAYYRRAQQVRTLIRRDFQEVWSKV
DLLAAPTSPSVAFKIGEKADDPVAMYLSDVCTLPINLAGVPGLVVPCGFSDNLPTGLQLIGKPFDEATLLRVGHAYEQNN
SWHKQHPNL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=494, Percent_Identity=36.6396761133603, Blast_Score=312, Evalue=5e-85,
Organism=Homo sapiens, GI195972892, Length=479, Percent_Identity=25.2609603340292, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI166795287, Length=238, Percent_Identity=31.9327731092437, Blast_Score=76, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=436, Percent_Identity=38.7614678899083, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI17537465, Length=499, Percent_Identity=26.4529058116232, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI71990152, Length=319, Percent_Identity=31.3479623824451, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=263, Percent_Identity=30.4182509505703, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17538252, Length=318, Percent_Identity=27.6729559748428, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17556276, Length=278, Percent_Identity=31.294964028777, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17556278, Length=278, Percent_Identity=31.294964028777, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17538254, Length=336, Percent_Identity=24.1071428571429, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=427, Percent_Identity=39.1100702576112, Blast_Score=263, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6319685, Length=462, Percent_Identity=29.2207792207792, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6320448, Length=232, Percent_Identity=28.448275862069, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24648113, Length=496, Percent_Identity=39.1129032258064, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24652985, Length=485, Percent_Identity=27.6288659793814, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI19922090, Length=485, Percent_Identity=27.6288659793814, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24652981, Length=485, Percent_Identity=27.6288659793814, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24652983, Length=485, Percent_Identity=27.6288659793814, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24644968, Length=494, Percent_Identity=26.3157894736842, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161078093, Length=514, Percent_Identity=27.431906614786, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI45550774, Length=480, Percent_Identity=25.4166666666667, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648435, Length=480, Percent_Identity=25.4166666666667, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648437, Length=480, Percent_Identity=25.4166666666667, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648441, Length=423, Percent_Identity=26.0047281323877, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24648439, Length=423, Percent_Identity=26.0047281323877, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21356731, Length=486, Percent_Identity=24.2798353909465, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52518; Mature: 52518

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREDVIMALHELTIAEARAQLDRGEITSVQLTEALLERIAALEPTLHSFLTQTPDLALQQ
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHH
AQAADERIKAGNATSLTGIPLGIKDVIVTKDVRTTCGSKVLENWVPPYDATVVSKLNEAG
HHHHHHHHHCCCCCEECCCCCCHHHEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
TVTLGKLNMDEFAMGSSNENSAFGGTRNPWDTERVPGGSSGGSAAAVAAGQAFGTLGTDT
CEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEECCCHHCCCCCCC
GGSIRQPAALCGIVGLKPTYGRVSRYGLVAFASSLDQCGPFARTVEDVALLLETIAGHDP
CCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
KDSTSANLPVPDYRASLTGDIRGLKVGVPSEYFVDGMEPGVEKAVRTAIEQIKDLGAEIV
CCCCCCCCCCCCCHHCCCCCCCEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHCCHHEE
EVSLPHTKYALPVYYIIAPAECSTNLARFDGVRYGVRLEGNGLFDEIESTRGQLFGAEVR
EEECCCCCEECEEEEEEECCHHCCCHHHHCCEEEEEEECCCCCHHHHHHHHHHEEHHHHH
RRIMLGTYALSSGYYDAYYRRAQQVRTLIRRDFQEVWSKVDLLAAPTSPSVAFKIGEKAD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEECCCCC
DPVAMYLSDVCTLPINLAGVPGLVVPCGFSDNLPTGLQLIGKPFDEATLLRVGHAYEQNN
CHHHHHHHHHHHCCEEECCCCCEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCC
SWHKQHPNL
CCCCCCCCC
>Mature Secondary Structure
MREDVIMALHELTIAEARAQLDRGEITSVQLTEALLERIAALEPTLHSFLTQTPDLALQQ
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHH
AQAADERIKAGNATSLTGIPLGIKDVIVTKDVRTTCGSKVLENWVPPYDATVVSKLNEAG
HHHHHHHHHCCCCCEECCCCCCHHHEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
TVTLGKLNMDEFAMGSSNENSAFGGTRNPWDTERVPGGSSGGSAAAVAAGQAFGTLGTDT
CEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEECCCHHCCCCCCC
GGSIRQPAALCGIVGLKPTYGRVSRYGLVAFASSLDQCGPFARTVEDVALLLETIAGHDP
CCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
KDSTSANLPVPDYRASLTGDIRGLKVGVPSEYFVDGMEPGVEKAVRTAIEQIKDLGAEIV
CCCCCCCCCCCCCHHCCCCCCCEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHCCHHEE
EVSLPHTKYALPVYYIIAPAECSTNLARFDGVRYGVRLEGNGLFDEIESTRGQLFGAEVR
EEECCCCCEECEEEEEEECCHHCCCHHHHCCEEEEEEECCCCCHHHHHHHHHHEEHHHHH
RRIMLGTYALSSGYYDAYYRRAQQVRTLIRRDFQEVWSKVDLLAAPTSPSVAFKIGEKAD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEECCCCC
DPVAMYLSDVCTLPINLAGVPGLVVPCGFSDNLPTGLQLIGKPFDEATLLRVGHAYEQNN
CHHHHHHHHHHHCCEEECCCCCEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCC
SWHKQHPNL
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA