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The map label for this gene is tnsA [H]

Identifier: 159900464

GI number: 159900464

Start: 4954270

End: 4955100

Strand: Direct

Name: tnsA [H]

Synonym: Haur_3949

Alternate gene names: 159900464

Gene position: 4954270-4955100 (Clockwise)

Preceding gene: 159900463

Following gene: 159900465

Centisome position: 78.06

GC content: 44.28

Gene sequence:

>831_bases
ATGGCAAAAAGGCGAAGAGCCACAACACATGAATCCATCCAGAAGCTCATTGCAGCAGGCCGTGGGCAAAACGAAGGACG
GAAATATAAGCCTTGGTTGACTATTCAGGATGTACCGTCTAATGGTCTAGTCCATCGAATTAAAGGAAGTAAACATGGAC
GTGTGCATCATTTAATGAGCAATTTGGAGCGTGACTACTTTTACTTACTTGATTGGGCTGACGATGTTATTGATATTCGC
GAACAGTATCCCCTTCTGCCTATCGAGGAAACACAGAATATTGCAAAATCATGCAGCATAAAGCATCCACGCGATCCTGC
AACGAAACATGACATTGTGATGACTACCGACTTTGTTATTACCATGGAGAGAGATGGAAAACGCTGGATTCAAGCCCGAA
CCGTAAAACCATCAGAGAAGCTACGTTCCCAGCGAGTTCTCGAGAAGTTTGAGATCGAACGGAAATATTGGGAAACACGA
GGAATTAACTGGGGAATTGTCACCGAGTTGGATATTCCATCCGTCACAGTTCGCAATATCGATGTGTTGCGACTTTATCG
GACGATAACAGATCGTGGCCTTTCAGGCACGGAAATTCAGTCTATTGCCGATTACTTACGCCGCAATTTGCGGAATGCAT
TGTTTACATTGGCGCAAATTACTACTGAATGTGATGCTGCGATGAGAGTTCGTCCAGGTAGTAGCCTCGTCGTTGCGTAT
CATTTGATTGCAACGCGTAAATGGATAATTGATCTCCACCGTCCGTTTAATCCCACAGAACTTTTTATACTCCAAGAACG
AAAGGACTCCTATGGATCTCTGCCTAAATAG

Upstream 100 bases:

>100_bases
CTATAAAACAAGCCATAAAATTTCGTATTGACTCATATAGGATCATGGTGTATTTTTTAGGCGCAATAATTCACATTTAT
TAAAAAATGAAAGGAAATCT

Downstream 100 bases:

>100_bases
TCTACTTTCTTGGCATGACGAAGATAAAAAACCAAGAATTGAACGGGTTCTCTATCTTAACCCTGTCTATGATCTTATCT
ATACAATTGATATACATAGT

Product: TnsA endonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MAKRRRATTHESIQKLIAAGRGQNEGRKYKPWLTIQDVPSNGLVHRIKGSKHGRVHHLMSNLERDYFYLLDWADDVIDIR
EQYPLLPIEETQNIAKSCSIKHPRDPATKHDIVMTTDFVITMERDGKRWIQARTVKPSEKLRSQRVLEKFEIERKYWETR
GINWGIVTELDIPSVTVRNIDVLRLYRTITDRGLSGTEIQSIADYLRRNLRNALFTLAQITTECDAAMRVRPGSSLVVAY
HLIATRKWIIDLHRPFNPTELFILQERKDSYGSLPK

Sequences:

>Translated_276_residues
MAKRRRATTHESIQKLIAAGRGQNEGRKYKPWLTIQDVPSNGLVHRIKGSKHGRVHHLMSNLERDYFYLLDWADDVIDIR
EQYPLLPIEETQNIAKSCSIKHPRDPATKHDIVMTTDFVITMERDGKRWIQARTVKPSEKLRSQRVLEKFEIERKYWETR
GINWGIVTELDIPSVTVRNIDVLRLYRTITDRGLSGTEIQSIADYLRRNLRNALFTLAQITTECDAAMRVRPGSSLVVAY
HLIATRKWIIDLHRPFNPTELFILQERKDSYGSLPK
>Mature_275_residues
AKRRRATTHESIQKLIAAGRGQNEGRKYKPWLTIQDVPSNGLVHRIKGSKHGRVHHLMSNLERDYFYLLDWADDVIDIRE
QYPLLPIEETQNIAKSCSIKHPRDPATKHDIVMTTDFVITMERDGKRWIQARTVKPSEKLRSQRVLEKFEIERKYWETRG
INWGIVTELDIPSVTVRNIDVLRLYRTITDRGLSGTEIQSIADYLRRNLRNALFTLAQITTECDAAMRVRPGSSLVVAYH
LIATRKWIIDLHRPFNPTELFILQERKDSYGSLPK

Specific function: Required for Tn7 transposition. Forms the transposase, together with TnsB. TnsA executes the 5'-DNA strand breakage reaction. TnsABC and tnsD promote high-frequency insertion of Tn7 into a specific target site known as ATT-Tn7 whereas tnsABC and tnsD prom

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011856
- InterPro:   IPR011335
- InterPro:   IPR014832
- InterPro:   IPR014833
- InterPro:   IPR011991 [H]

Pfam domain/function: PF08721 TnsA_C; PF08722 TnsA_N [H]

EC number: NA

Molecular weight: Translated: 32199; Mature: 32067

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRRRATTHESIQKLIAAGRGQNEGRKYKPWLTIQDVPSNGLVHRIKGSKHGRVHHLMS
CCCCCCCHHHHHHHHHHHHCCCCCCCCEECCEEEEEECCCCCCEEEECCCCCCHHHHHHH
NLERDYFYLLDWADDVIDIREQYPLLPIEETQNIAKSCSIKHPRDPATKHDIVMTTDFVI
HCCCCEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEE
TMERDGKRWIQARTVKPSEKLRSQRVLEKFEIERKYWETRGINWGIVTELDIPSVTVRNI
EEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCH
DVLRLYRTITDRGLSGTEIQSIADYLRRNLRNALFTLAQITTECDAAMRVRPGSSLVVAY
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEHHH
HLIATRKWIIDLHRPFNPTELFILQERKDSYGSLPK
HHHHHHHHEEEECCCCCCEEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
AKRRRATTHESIQKLIAAGRGQNEGRKYKPWLTIQDVPSNGLVHRIKGSKHGRVHHLMS
CCCCCCHHHHHHHHHHHHCCCCCCCCEECCEEEEEECCCCCCEEEECCCCCCHHHHHHH
NLERDYFYLLDWADDVIDIREQYPLLPIEETQNIAKSCSIKHPRDPATKHDIVMTTDFVI
HCCCCEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEE
TMERDGKRWIQARTVKPSEKLRSQRVLEKFEIERKYWETRGINWGIVTELDIPSVTVRNI
EEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCH
DVLRLYRTITDRGLSGTEIQSIADYLRRNLRNALFTLAQITTECDAAMRVRPGSSLVVAY
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEHHH
HLIATRKWIIDLHRPFNPTELFILQERKDSYGSLPK
HHHHHHHHEEEECCCCCCEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2156235; 1655576; 3010949; 8947057; 10704304 [H]