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The map label for this gene is pheS

Identifier: 159900353

GI number: 159900353

Start: 4816182

End: 4817222

Strand: Direct

Name: pheS

Synonym: Haur_3836

Alternate gene names: 159900353

Gene position: 4816182-4817222 (Clockwise)

Preceding gene: 159900352

Following gene: 159900354

Centisome position: 75.89

GC content: 52.45

Gene sequence:

>1041_bases
ATGTTTGAGCAATTAGATCAAATTGAAAAAGAGGCCGCTGCGGCCTTAGCCAGCGTCCAATCACTCGATGATTTGGCTGC
ATGGCGCACCCAATGGACTGGCAAAAAGGGCGCGTTGGCCCAAGCCAGTCAATCAATCGGTAAGCTCGACCCCAAAGATC
GGCCAGCCTTTGGCCAACGCTTTGGCGCGATCAAGCAAGCCTTGAGCGAACAAGAAACCACTTTAGAAGCGCGTTTGCAA
AGCGCGGCCTTGCATCAAGAGCTTGAAGAAGACGCGGTTGACATTAGTTTGCCTGGGCGAGCTGCCAATATTGGCCGTTT
GCACCCCAGCACTCAATCGCTGCGCCGAATTCAGCATATTTTTGCTGAAATGGGCTTTCAAGTCTGGGAAAGCCGCGAAG
TCGAATCCGATGAGTATAATTTTGAATTGCTCAATATGCCGGCGCATCACCCAGCCCGCGATATGTGGGATACGTTCTAT
GTGCAATCCGACGATCCTCATCAAAAGGTCGTGTTGCGCACCCACACCTCGCCTGGCCAAATTCATGTGATGCGAACATT
GAATCCTGAGCCAATTCGGGTGATTTTGCCTGGTAAGTGCTATCGCTACGAGCCAGTCAGCGCTCGATCCGAGATGATGT
TCCATCAAGTTGAAGGCTTGGTGATCGGCAAAAACATTACCATGGCCGACCTCAAAGGTACCTTGGCCAACTTTGCGCGG
CGCATGTTTAAGGATGATGTGAAAGTGCGTTATCGCCCTTCATACTTCCCCTTCACCGAGCCAAGCGTCGAAGTCGATAT
CGAATGTTTCATTTGTGGTGGCGAAGGCTGTCGGATTTGCAAAAAGAGCGGCTGGCTGGAAATTCTGGGCGCAGGGATGG
TGCACCCAACCGTCTTGCGCAACGGCGGCTATGATCCCGCTGAGTGGAGCGGCTTTGCCTTTGGGATGGGGCCAGAACGC
CAAACCATGCTGCGCTACGACATCGACGACATTCGCTGGTTCTTCTCCAACGATGGTCGGTTCTTGGAACAATTCGGTTA
A

Upstream 100 bases:

>100_bases
TTGGTTAGTGTTGATGGTATTCAACTGCGCGATTTAAATGAACAGAAACCCCAATGAGTGTGCTACACTACGCGGAGCAT
ATTAAAAGGTAGGAATGGCA

Downstream 100 bases:

>100_bases
TTCTCCGATCGGCGCTGAATGAGACCATTCAGCGCCTTGCTTCAATGCCTGCATTACAACGCTTCCGCTATCATTGGCCT
TTGCTGCTCCTCCTGCTGGT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MFEQLDQIEKEAAAALASVQSLDDLAAWRTQWTGKKGALAQASQSIGKLDPKDRPAFGQRFGAIKQALSEQETTLEARLQ
SAALHQELEEDAVDISLPGRAANIGRLHPSTQSLRRIQHIFAEMGFQVWESREVESDEYNFELLNMPAHHPARDMWDTFY
VQSDDPHQKVVLRTHTSPGQIHVMRTLNPEPIRVILPGKCYRYEPVSARSEMMFHQVEGLVIGKNITMADLKGTLANFAR
RMFKDDVKVRYRPSYFPFTEPSVEVDIECFICGGEGCRICKKSGWLEILGAGMVHPTVLRNGGYDPAEWSGFAFGMGPER
QTMLRYDIDDIRWFFSNDGRFLEQFG

Sequences:

>Translated_346_residues
MFEQLDQIEKEAAAALASVQSLDDLAAWRTQWTGKKGALAQASQSIGKLDPKDRPAFGQRFGAIKQALSEQETTLEARLQ
SAALHQELEEDAVDISLPGRAANIGRLHPSTQSLRRIQHIFAEMGFQVWESREVESDEYNFELLNMPAHHPARDMWDTFY
VQSDDPHQKVVLRTHTSPGQIHVMRTLNPEPIRVILPGKCYRYEPVSARSEMMFHQVEGLVIGKNITMADLKGTLANFAR
RMFKDDVKVRYRPSYFPFTEPSVEVDIECFICGGEGCRICKKSGWLEILGAGMVHPTVLRNGGYDPAEWSGFAFGMGPER
QTMLRYDIDDIRWFFSNDGRFLEQFG
>Mature_346_residues
MFEQLDQIEKEAAAALASVQSLDDLAAWRTQWTGKKGALAQASQSIGKLDPKDRPAFGQRFGAIKQALSEQETTLEARLQ
SAALHQELEEDAVDISLPGRAANIGRLHPSTQSLRRIQHIFAEMGFQVWESREVESDEYNFELLNMPAHHPARDMWDTFY
VQSDDPHQKVVLRTHTSPGQIHVMRTLNPEPIRVILPGKCYRYEPVSARSEMMFHQVEGLVIGKNITMADLKGTLANFAR
RMFKDDVKVRYRPSYFPFTEPSVEVDIECFICGGEGCRICKKSGWLEILGAGMVHPTVLRNGGYDPAEWSGFAFGMGPER
QTMLRYDIDDIRWFFSNDGRFLEQFG

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=309, Percent_Identity=27.1844660194175, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI5729820, Length=241, Percent_Identity=30.2904564315353, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1788007, Length=344, Percent_Identity=44.7674418604651, Blast_Score=296, Evalue=9e-82,
Organism=Caenorhabditis elegans, GI17508957, Length=309, Percent_Identity=28.8025889967638, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI32563657, Length=309, Percent_Identity=28.8025889967638, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI32566635, Length=263, Percent_Identity=31.5589353612167, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321087, Length=369, Percent_Identity=28.1842818428184, Blast_Score=132, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6325304, Length=333, Percent_Identity=24.6246246246246, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI18858079, Length=306, Percent_Identity=29.0849673202614, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17137424, Length=271, Percent_Identity=27.3062730627306, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_HERA2 (A9B8B9)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001546600.1
- ProteinModelPortal:   A9B8B9
- SMR:   A9B8B9
- GeneID:   5735701
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_3836
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   HAUR316274:HAUR_3836-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 39289; Mature: 39289

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEQLDQIEKEAAAALASVQSLDDLAAWRTQWTGKKGALAQASQSIGKLDPKDRPAFGQR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHH
FGAIKQALSEQETTLEARLQSAALHQELEEDAVDISLPGRAANIGRLHPSTQSLRRIQHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHHH
FAEMGFQVWESREVESDEYNFELLNMPAHHPARDMWDTFYVQSDDPHQKVVLRTHTSPGQ
HHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHEEEECCCCCCCEEEEEECCCCCE
IHVMRTLNPEPIRVILPGKCYRYEPVSARSEMMFHQVEGLVIGKNITMADLKGTLANFAR
EEEEEECCCCCEEEEECCCCEEECCCCHHHHHHHHHHCCEEEECCEEHHHHHHHHHHHHH
RMFKDDVKVRYRPSYFPFTEPSVEVDIECFICGGEGCRICKKSGWLEILGAGMVHPTVLR
HHHHCCCCEEECCCCCCCCCCCEEEEEEEEEECCCCCEEHHHCCCCEEECCCCCCHHHHC
NGGYDPAEWSGFAFGMGPERQTMLRYDIDDIRWFFSNDGRFLEQFG
CCCCCCCCCCCEEECCCCCCCHHEEECHHHHEEEECCCCHHHHHCC
>Mature Secondary Structure
MFEQLDQIEKEAAAALASVQSLDDLAAWRTQWTGKKGALAQASQSIGKLDPKDRPAFGQR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHH
FGAIKQALSEQETTLEARLQSAALHQELEEDAVDISLPGRAANIGRLHPSTQSLRRIQHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHHH
FAEMGFQVWESREVESDEYNFELLNMPAHHPARDMWDTFYVQSDDPHQKVVLRTHTSPGQ
HHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHEEEECCCCCCCEEEEEECCCCCE
IHVMRTLNPEPIRVILPGKCYRYEPVSARSEMMFHQVEGLVIGKNITMADLKGTLANFAR
EEEEEECCCCCEEEEECCCCEEECCCCHHHHHHHHHHCCEEEECCEEHHHHHHHHHHHHH
RMFKDDVKVRYRPSYFPFTEPSVEVDIECFICGGEGCRICKKSGWLEILGAGMVHPTVLR
HHHHCCCCEEECCCCCCCCCCCEEEEEEEEEECCCCCEEHHHCCCCEEECCCCCCHHHHC
NGGYDPAEWSGFAFGMGPERQTMLRYDIDDIRWFFSNDGRFLEQFG
CCCCCCCCCCCEEECCCCCCCHHEEECHHHHEEEECCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA