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The map label for this gene is hisS [H]

Identifier: 159900352

GI number: 159900352

Start: 4814738

End: 4816138

Strand: Direct

Name: hisS [H]

Synonym: Haur_3835

Alternate gene names: 159900352

Gene position: 4814738-4816138 (Clockwise)

Preceding gene: 159900349

Following gene: 159900353

Centisome position: 75.86

GC content: 53.53

Gene sequence:

>1401_bases
GTGGGCTATCAATTAACTCCTGAAGTGGTTCGCGGAACCCGTGACCTTTTTGCCGCTGCCGTTCACCAACGTCAGGCGCT
GATTCAGACATTAACCGCTACTTTTGATCAAGCTGGCTATGACCCGATTGAGTTGCCATTGCTTGAGCATCGTGAGTTGT
ATTTAAAAAAATCTGGTGATGATCTCATCGCCAAATTGTACCATTGGAATCAAGGTGGCCGTGATTTGGCCTTACGCCCT
GAATGGACAGCCTCGGTCTTACGAGCAGTCATCAGTGGCATGGGCGATGCGCCAATTCCCTTACGCTTGCGCTACGCTGG
GCCAGTGTTTCGCTATGAACGCCCACGTCGCGCGACCTATCGGCAATTTACCCAAGTTGGCATCGAATTAATCGGTGCAC
CTGGCCCGTTGGCCGATGCTGAAGCCCTTGGTTTGGCTGTCACTGGCCTGCGCGAATTGGGCATTCAGCAATGGACGTTA
ACCATTGGCCATATTGGTGTAATTAAGACGTTGCTCAATAGCCTCGGCTTACCCGAACGGATCACGTCGGCGCTCACCTG
GAGCCTCGAACGGATTCGTTCTAAAGGGCTTGATGCGGTTAAACAACAATGGCGCGACGACGACGACGATCTGCCAGTTG
ATTTAGCCAGCCTAGCCCATCTCGCCGACCAAGATCTTGAGACGCTGTTGTTGCGCGTATTACCCAGTCTTGGGGTGCGC
CTCGATAGCGGCGGGCGTGAGCCACAAGCAATCATTCAACGCTTGGTGCGCAAGCTGCGCCGTGGCGACGATGCGCTTAA
TCTTGATCGCGCATGGCAGTTGCTTTCAGCTTTAACCGCTGCCCGTGGCTCGGCACCAGATGTGATGCAACAGATGCGCG
AGCTATGCCAAGAATATACGGTTGCACCCGACGCTTTGGATGAGTTGCAAACCACCCTGACCTTACTTGAGGCATATGGC
GTGCCAGCAGATCAGATTGTGCTGGATTTTGGCATGGGTCGTGGCTTGCACTACTACACTGGCCTGATTTTCGAGATCGA
TGGCGCTGATGGCTTGCAACTCTGTGGCGGTGGCCGCTATGATGATTTAGTTGCGGCGCTTGGTGGGCGAGCAATGCCAG
CCGTTGGCTTTGCCTATGGACTCGAACGAATCGTCGCAGCAGTTGCGCCAGCCGAAATTGCCCCAGTTAAAAGTGTGTTG
GTTGTTGGCGATGATCATGGCTTGGTAATTCAGGCAGCAGCAGCACTCCGCCAACAAGGCTATCGCACGGCAGTTGATTT
GCGCCAGCGTTCGTATGCGGCCAATTTAAATGATGCCCGTCGGCGTGAAATGAGCCATCTGGCCTTGGTTAGTGTTGATG
GTATTCAACTGCGCGATTTAAATGAACAGAAACCCCAATGA

Upstream 100 bases:

>100_bases
AGGCAGACATAAGTACTTTACGCTATACGACCCTATTTGGTTCCGTTCACCCTTGCGAAATTTTCGCGTACAATGGTTGC
GATTGTGCTCGGAGGAACCT

Downstream 100 bases:

>100_bases
GTGTGCTACACTACGCGGAGCATATTAAAAGGTAGGAATGGCAATGTTTGAGCAATTAGATCAAATTGAAAAAGAGGCCG
CTGCGGCCTTAGCCAGCGTC

Product: histidine--tRNA ligase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MGYQLTPEVVRGTRDLFAAAVHQRQALIQTLTATFDQAGYDPIELPLLEHRELYLKKSGDDLIAKLYHWNQGGRDLALRP
EWTASVLRAVISGMGDAPIPLRLRYAGPVFRYERPRRATYRQFTQVGIELIGAPGPLADAEALGLAVTGLRELGIQQWTL
TIGHIGVIKTLLNSLGLPERITSALTWSLERIRSKGLDAVKQQWRDDDDDLPVDLASLAHLADQDLETLLLRVLPSLGVR
LDSGGREPQAIIQRLVRKLRRGDDALNLDRAWQLLSALTAARGSAPDVMQQMRELCQEYTVAPDALDELQTTLTLLEAYG
VPADQIVLDFGMGRGLHYYTGLIFEIDGADGLQLCGGGRYDDLVAALGGRAMPAVGFAYGLERIVAAVAPAEIAPVKSVL
VVGDDHGLVIQAAAALRQQGYRTAVDLRQRSYAANLNDARRREMSHLALVSVDGIQLRDLNEQKPQ

Sequences:

>Translated_466_residues
MGYQLTPEVVRGTRDLFAAAVHQRQALIQTLTATFDQAGYDPIELPLLEHRELYLKKSGDDLIAKLYHWNQGGRDLALRP
EWTASVLRAVISGMGDAPIPLRLRYAGPVFRYERPRRATYRQFTQVGIELIGAPGPLADAEALGLAVTGLRELGIQQWTL
TIGHIGVIKTLLNSLGLPERITSALTWSLERIRSKGLDAVKQQWRDDDDDLPVDLASLAHLADQDLETLLLRVLPSLGVR
LDSGGREPQAIIQRLVRKLRRGDDALNLDRAWQLLSALTAARGSAPDVMQQMRELCQEYTVAPDALDELQTTLTLLEAYG
VPADQIVLDFGMGRGLHYYTGLIFEIDGADGLQLCGGGRYDDLVAALGGRAMPAVGFAYGLERIVAAVAPAEIAPVKSVL
VVGDDHGLVIQAAAALRQQGYRTAVDLRQRSYAANLNDARRREMSHLALVSVDGIQLRDLNEQKPQ
>Mature_465_residues
GYQLTPEVVRGTRDLFAAAVHQRQALIQTLTATFDQAGYDPIELPLLEHRELYLKKSGDDLIAKLYHWNQGGRDLALRPE
WTASVLRAVISGMGDAPIPLRLRYAGPVFRYERPRRATYRQFTQVGIELIGAPGPLADAEALGLAVTGLRELGIQQWTLT
IGHIGVIKTLLNSLGLPERITSALTWSLERIRSKGLDAVKQQWRDDDDDLPVDLASLAHLADQDLETLLLRVLPSLGVRL
DSGGREPQAIIQRLVRKLRRGDDALNLDRAWQLLSALTAARGSAPDVMQQMRELCQEYTVAPDALDELQTTLTLLEAYGV
PADQIVLDFGMGRGLHYYTGLIFEIDGADGLQLCGGGRYDDLVAALGGRAMPAVGFAYGLERIVAAVAPAEIAPVKSVLV
VGDDHGLVIQAAAALRQQGYRTAVDLRQRSYAANLNDARRREMSHLALVSVDGIQLRDLNEQKPQ

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=418, Percent_Identity=31.3397129186603, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71993693, Length=449, Percent_Identity=23.8307349665924, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71993686, Length=449, Percent_Identity=23.8307349665924, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325290, Length=421, Percent_Identity=22.8028503562945, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 51166; Mature: 51035

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYQLTPEVVRGTRDLFAAAVHQRQALIQTLTATFDQAGYDPIELPLLEHRELYLKKSGD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCH
DLIAKLYHWNQGGRDLALRPEWTASVLRAVISGMGDAPIPLRLRYAGPVFRYERPRRATY
HHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCCCCHHHH
RQFTQVGIELIGAPGPLADAEALGLAVTGLRELGIQQWTLTIGHIGVIKTLLNSLGLPER
HHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHCCCHHEEEHHHHHHHHHHHHHHCCCHHH
ITSALTWSLERIRSKGLDAVKQQWRDDDDDLPVDLASLAHLADQDLETLLLRVLPSLGVR
HHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEE
LDSGGREPQAIIQRLVRKLRRGDDALNLDRAWQLLSALTAARGSAPDVMQQMRELCQEYT
ECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
VAPDALDELQTTLTLLEAYGVPADQIVLDFGMGRGLHYYTGLIFEIDGADGLQLCGGGRY
CCHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCHHHCCEEEEECCCCCCEECCCCCH
DDLVAALGGRAMPAVGFAYGLERIVAAVAPAEIAPVKSVLVVGDDHGLVIQAAAALRQQG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHCCCCEEEEEECCCCHHHHHHHHHHHCC
YRTAVDLRQRSYAANLNDARRREMSHLALVSVDGIQLRDLNEQKPQ
CHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCEEEECCCCCCCC
>Mature Secondary Structure 
GYQLTPEVVRGTRDLFAAAVHQRQALIQTLTATFDQAGYDPIELPLLEHRELYLKKSGD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCH
DLIAKLYHWNQGGRDLALRPEWTASVLRAVISGMGDAPIPLRLRYAGPVFRYERPRRATY
HHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCCCCHHHH
RQFTQVGIELIGAPGPLADAEALGLAVTGLRELGIQQWTLTIGHIGVIKTLLNSLGLPER
HHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHCCCHHEEEHHHHHHHHHHHHHHCCCHHH
ITSALTWSLERIRSKGLDAVKQQWRDDDDDLPVDLASLAHLADQDLETLLLRVLPSLGVR
HHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEE
LDSGGREPQAIIQRLVRKLRRGDDALNLDRAWQLLSALTAARGSAPDVMQQMRELCQEYT
ECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
VAPDALDELQTTLTLLEAYGVPADQIVLDFGMGRGLHYYTGLIFEIDGADGLQLCGGGRY
CCHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCHHHCCEEEEECCCCCCEECCCCCH
DDLVAALGGRAMPAVGFAYGLERIVAAVAPAEIAPVKSVLVVGDDHGLVIQAAAALRQQG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHCCCCEEEEEECCCCHHHHHHHHHHHCC
YRTAVDLRQRSYAANLNDARRREMSHLALVSVDGIQLRDLNEQKPQ
CHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA