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The map label for this gene is ada [H]

Identifier: 159900272

GI number: 159900272

Start: 4721122

End: 4722591

Strand: Direct

Name: ada [H]

Synonym: Haur_3755

Alternate gene names: 159900272

Gene position: 4721122-4722591 (Clockwise)

Preceding gene: 159900271

Following gene: 159900273

Centisome position: 74.39

GC content: 50.68

Gene sequence:

>1470_bases
ATGCTGATGTTAACTGAAATCGAGCCAAGTCTGCCAAGCCACACCGAAATGGTTGCCCACATGCTGCAATCGGATGCCAG
CTATAACGGCAAATTTATTACGGCGGTCAAAACTACGGGCATCTATTGTTTGCCAAGTTGCCGCGCCCGTAAGCCCAAGC
CTGAGAATGTTGAATTTTTTACCAATCCCAACGCAGCTCAAGGTGCGGGCTATCGCGCTTGCAAATTGTGCCGCCCTGAT
GATTTTTATCGTGGCTTTGACCCTGAGGAACATTTGACTGAGCAATTGATTGAAGCGGTGCTGGCCCAACCAGCCGCATT
TGCCGATGTCAAGGCCATGGCGCAAACAGCGGGGGTTGGCCAAACCAAATTATTTGAATTAATGCGCATTTATTACCACA
CCACACCCGCCGATCTACTACTACGAGCGCGAATTGAGGCCGCTTGTGGTTTATTGCTCAACACCGATCAAACGATTATT
GCGATCGCTAATGAAGTGGGCTTCGATAGCTTATCGAGCTTCAATGAAAACTTTCGCAAACACACTATGCTCACGCCTAG
CGAATATCGGCGTATATCTGAAACTGGGCGATTTAGCCTTGCCTTGCCCAACGATTATCCTAGCCGCCAAATTTTAGGCC
AGCTTGGGCGCGATCCAGTTAGCCTGACCGATCAGGTGGTCGAGCAAACATGGTACAGCACCTGTCGCTTAAATGGGCAA
ACTGGGGTGTTACTCGCAGTCACTATCACTCCAACCACGGCTGAATGCAGCATCGTGGAGCAATCAGCCGTAACGCCCAG
CGACGTTGCCACGATTCATCGCCATGTTATTGCAGGCTTGGGTTTGAGTAACGATCCCAGTCGTTTCGAGGCCCATGTTG
CCAAATCGCCCGCCTTATTGCCATTAATTGAGCACCAACGTGGTTTGCGCATGCCCTTGGTGCATAATCCATTCGATGCC
TTGGTTTGGGCAATTTTGGGTCAGCAAATTTCGCTGGCGGTGGCTTATCGTTTGCGCCAACGCCTAACCGAGCTAGTTGG
GCAACGATTAAATCAAGATTTTTATCTTGCGCCAACGCCCAATACAATTGCCCAACTAACCGTTGAGCAACTGCTACCCT
TGGGCTTTTCCAACGCCAAAGCCCGCTATTTAATTGATACCGCCCAGGCGATTATCGCTGAAAGCTTGCCATTGGCGAGC
TATCACCGCAAATCGGCCACACGGATCGAGCGCGAACTACTAGCGTTGCGGGGCATCGGCCCATGGACAGCCCAATATGT
ACTAATGCGTTCGTTTGGCTTTAGCGATTGTGTACCAGTGGGCGATAGTGGCCTGACCAGCAGCTTACAGGCATTTTTTC
AGCTTGAGCAACGCCCCGATCGCTCGACAACCCTTGCTTTGATGGCAGCATTTAGCCCTTATCGCAGCCTAGCAACCTTT
CATTTATGGCAACGTTTGAAGCCAATGTGA

Upstream 100 bases:

>100_bases
ACGCTGGTGGTTTGGAACGCAAACGCTGGTTGCTGCGCCACGAGGGTGCTCAATTTAAAGATACGCCTGCCCAAGCGAGT
TTTGAATTAGTTTGAAAGAT

Downstream 100 bases:

>100_bases
GGGCAGAAGTTCGTATTTTTGATCCACGAAGGGCACAAAGGACACGAAGAACGTAAAAGTTTGGGTTAATAGGCTAGCTG
TTTGGATTACCAACAAATGC

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MLMLTEIEPSLPSHTEMVAHMLQSDASYNGKFITAVKTTGIYCLPSCRARKPKPENVEFFTNPNAAQGAGYRACKLCRPD
DFYRGFDPEEHLTEQLIEAVLAQPAAFADVKAMAQTAGVGQTKLFELMRIYYHTTPADLLLRARIEAACGLLLNTDQTII
AIANEVGFDSLSSFNENFRKHTMLTPSEYRRISETGRFSLALPNDYPSRQILGQLGRDPVSLTDQVVEQTWYSTCRLNGQ
TGVLLAVTITPTTAECSIVEQSAVTPSDVATIHRHVIAGLGLSNDPSRFEAHVAKSPALLPLIEHQRGLRMPLVHNPFDA
LVWAILGQQISLAVAYRLRQRLTELVGQRLNQDFYLAPTPNTIAQLTVEQLLPLGFSNAKARYLIDTAQAIIAESLPLAS
YHRKSATRIERELLALRGIGPWTAQYVLMRSFGFSDCVPVGDSGLTSSLQAFFQLEQRPDRSTTLALMAAFSPYRSLATF
HLWQRLKPM

Sequences:

>Translated_489_residues
MLMLTEIEPSLPSHTEMVAHMLQSDASYNGKFITAVKTTGIYCLPSCRARKPKPENVEFFTNPNAAQGAGYRACKLCRPD
DFYRGFDPEEHLTEQLIEAVLAQPAAFADVKAMAQTAGVGQTKLFELMRIYYHTTPADLLLRARIEAACGLLLNTDQTII
AIANEVGFDSLSSFNENFRKHTMLTPSEYRRISETGRFSLALPNDYPSRQILGQLGRDPVSLTDQVVEQTWYSTCRLNGQ
TGVLLAVTITPTTAECSIVEQSAVTPSDVATIHRHVIAGLGLSNDPSRFEAHVAKSPALLPLIEHQRGLRMPLVHNPFDA
LVWAILGQQISLAVAYRLRQRLTELVGQRLNQDFYLAPTPNTIAQLTVEQLLPLGFSNAKARYLIDTAQAIIAESLPLAS
YHRKSATRIERELLALRGIGPWTAQYVLMRSFGFSDCVPVGDSGLTSSLQAFFQLEQRPDRSTTLALMAAFSPYRSLATF
HLWQRLKPM
>Mature_489_residues
MLMLTEIEPSLPSHTEMVAHMLQSDASYNGKFITAVKTTGIYCLPSCRARKPKPENVEFFTNPNAAQGAGYRACKLCRPD
DFYRGFDPEEHLTEQLIEAVLAQPAAFADVKAMAQTAGVGQTKLFELMRIYYHTTPADLLLRARIEAACGLLLNTDQTII
AIANEVGFDSLSSFNENFRKHTMLTPSEYRRISETGRFSLALPNDYPSRQILGQLGRDPVSLTDQVVEQTWYSTCRLNGQ
TGVLLAVTITPTTAECSIVEQSAVTPSDVATIHRHVIAGLGLSNDPSRFEAHVAKSPALLPLIEHQRGLRMPLVHNPFDA
LVWAILGQQISLAVAYRLRQRLTELVGQRLNQDFYLAPTPNTIAQLTVEQLLPLGFSNAKARYLIDTAQAIIAESLPLAS
YHRKSATRIERELLALRGIGPWTAQYVLMRSFGFSDCVPVGDSGLTSSLQAFFQLEQRPDRSTTLALMAAFSPYRSLATF
HLWQRLKPM

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=184, Percent_Identity=28.804347826087, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788383, Length=180, Percent_Identity=29.4444444444444, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 54299; Mature: 54299

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMLTEIEPSLPSHTEMVAHMLQSDASYNGKFITAVKTTGIYCLPSCRARKPKPENVEFF
CEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCEEECCCCCCCCCCCCCEEEE
TNPNAAQGAGYRACKLCRPDDFYRGFDPEEHLTEQLIEAVLAQPAAFADVKAMAQTAGVG
ECCCCCCCCCCCCEECCCCCHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
QTKLFELMRIYYHTTPADLLLRARIEAACGLLLNTDQTIIAIANEVGFDSLSSFNENFRK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCEEECCCCEEEEEECCCCHHHHHHHHHHHHH
HTMLTPSEYRRISETGRFSLALPNDYPSRQILGQLGRDPVSLTDQVVEQTWYSTCRLNGQ
HCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCC
TGVLLAVTITPTTAECSIVEQSAVTPSDVATIHRHVIAGLGLSNDPSRFEAHVAKSPALL
CCEEEEEEECCCCCCCEEHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
PLIEHQRGLRMPLVHNPFDALVWAILGQQISLAVAYRLRQRLTELVGQRLNQDFYLAPTP
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
NTIAQLTVEQLLPLGFSNAKARYLIDTAQAIIAESLPLASYHRKSATRIERELLALRGIG
CHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
PWTAQYVLMRSFGFSDCVPVGDSGLTSSLQAFFQLEQRPDRSTTLALMAAFSPYRSLATF
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
HLWQRLKPM
HHHHHHCCC
>Mature Secondary Structure
MLMLTEIEPSLPSHTEMVAHMLQSDASYNGKFITAVKTTGIYCLPSCRARKPKPENVEFF
CEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCEEECCCCCCCCCCCCCEEEE
TNPNAAQGAGYRACKLCRPDDFYRGFDPEEHLTEQLIEAVLAQPAAFADVKAMAQTAGVG
ECCCCCCCCCCCCEECCCCCHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
QTKLFELMRIYYHTTPADLLLRARIEAACGLLLNTDQTIIAIANEVGFDSLSSFNENFRK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCEEECCCCEEEEEECCCCHHHHHHHHHHHHH
HTMLTPSEYRRISETGRFSLALPNDYPSRQILGQLGRDPVSLTDQVVEQTWYSTCRLNGQ
HCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCC
TGVLLAVTITPTTAECSIVEQSAVTPSDVATIHRHVIAGLGLSNDPSRFEAHVAKSPALL
CCEEEEEEECCCCCCCEEHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
PLIEHQRGLRMPLVHNPFDALVWAILGQQISLAVAYRLRQRLTELVGQRLNQDFYLAPTP
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
NTIAQLTVEQLLPLGFSNAKARYLIDTAQAIIAESLPLASYHRKSATRIERELLALRGIG
CHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
PWTAQYVLMRSFGFSDCVPVGDSGLTSSLQAFFQLEQRPDRSTTLALMAAFSPYRSLATF
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
HLWQRLKPM
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]