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The map label for this gene is ogt [H]

Identifier: 159900271

GI number: 159900271

Start: 4720580

End: 4721116

Strand: Direct

Name: ogt [H]

Synonym: Haur_3754

Alternate gene names: 159900271

Gene position: 4720580-4721116 (Clockwise)

Preceding gene: 159900268

Following gene: 159900272

Centisome position: 74.38

GC content: 52.7

Gene sequence:

>537_bases
ATGCAACAGTTAACTATCGAACAGATCGCAACGCCGATTGGCGCTGTCGTGATTATTAGCAGCCCTCAAGGCGTAGTTTA
TTTAGATTTTGGCGATAACCAAGCTCGTTGCCAACGCTTGCTCAGCCAACGCTATGGCCAATTTGCGCTCATTCCGGCTA
GCCAAAATCTATGGGCCGATGCAGTTGCAAGCTATTTTGCAGGCGATCTGCGGGCGCTCGATGTGATTACGGTGGATCTG
GCAGGCAGCGAGTTTCAGTGCAAAGTTTGGTTGGCACTACGCGAAATCGGGGTTGGTCAAACGATGAGCTATGGCGAATT
GGCGGCCAAAGTTGGCACACCCAGCGCCGCCCGGGCGGTTGGCATGACCAACGGTCAAAATCCAATTAGCTTGGTGCTAC
CCTGCCATCGCGTGATCGGAGCCAACAAAAGCCTAACTGGCTACGCTGGTGGTTTGGAACGCAAACGCTGGTTGCTGCGC
CACGAGGGTGCTCAATTTAAAGATACGCCTGCCCAAGCGAGTTTTGAATTAGTTTGA

Upstream 100 bases:

>100_bases
AGGATATCTCGGCAAAAATTAGTGCGAAGAAAAACGGTAGCCGCCAGCAGCAACCCTCGCTATACTGCTTGCAGTCAATC
GGTCACACAGGAGTTTGCCC

Downstream 100 bases:

>100_bases
AAGATATGCTGATGTTAACTGAAATCGAGCCAAGTCTGCCAAGCCACACCGAAATGGTTGCCCACATGCTGCAATCGGAT
GCCAGCTATAACGGCAAATT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MQQLTIEQIATPIGAVVIISSPQGVVYLDFGDNQARCQRLLSQRYGQFALIPASQNLWADAVASYFAGDLRALDVITVDL
AGSEFQCKVWLALREIGVGQTMSYGELAAKVGTPSAARAVGMTNGQNPISLVLPCHRVIGANKSLTGYAGGLERKRWLLR
HEGAQFKDTPAQASFELV

Sequences:

>Translated_178_residues
MQQLTIEQIATPIGAVVIISSPQGVVYLDFGDNQARCQRLLSQRYGQFALIPASQNLWADAVASYFAGDLRALDVITVDL
AGSEFQCKVWLALREIGVGQTMSYGELAAKVGTPSAARAVGMTNGQNPISLVLPCHRVIGANKSLTGYAGGLERKRWLLR
HEGAQFKDTPAQASFELV
>Mature_178_residues
MQQLTIEQIATPIGAVVIISSPQGVVYLDFGDNQARCQRLLSQRYGQFALIPASQNLWADAVASYFAGDLRALDVITVDL
AGSEFQCKVWLALREIGVGQTMSYGELAAKVGTPSAARAVGMTNGQNPISLVLPCHRVIGANKSLTGYAGGLERKRWLLR
HEGAQFKDTPAQASFELV

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=78, Percent_Identity=43.5897435897436, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1787596, Length=167, Percent_Identity=43.1137724550898, Blast_Score=143, Evalue=8e-36,
Organism=Escherichia coli, GI1788542, Length=158, Percent_Identity=33.5443037974684, Blast_Score=89, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI115533068, Length=95, Percent_Identity=38.9473684210526, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=95, Percent_Identity=38.9473684210526, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=43.3734939759036, Blast_Score=85, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19179; Mature: 19179

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQLTIEQIATPIGAVVIISSPQGVVYLDFGDNQARCQRLLSQRYGQFALIPASQNLWAD
CCCCCHHHHCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCHHHH
AVASYFAGDLRALDVITVDLAGSEFQCKVWLALREIGVGQTMSYGELAAKVGTPSAARAV
HHHHHHHCCCEEEEEEEEEECCCCEEEEEEEEEHHCCCCCCCCHHHHHHHCCCCHHHHEE
GMTNGQNPISLVLPCHRVIGANKSLTGYAGGLERKRWLLRHEGAQFKDTPAQASFELV
CCCCCCCCEEEEEECHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEECC
>Mature Secondary Structure
MQQLTIEQIATPIGAVVIISSPQGVVYLDFGDNQARCQRLLSQRYGQFALIPASQNLWAD
CCCCCHHHHCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCHHHH
AVASYFAGDLRALDVITVDLAGSEFQCKVWLALREIGVGQTMSYGELAAKVGTPSAARAV
HHHHHHHCCCEEEEEEEEEECCCCEEEEEEEEEHHCCCCCCCCHHHHHHHCCCCHHHHEE
GMTNGQNPISLVLPCHRVIGANKSLTGYAGGLERKRWLLRHEGAQFKDTPAQASFELV
CCCCCCCCEEEEEECHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]