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The map label for this gene is rho [H]

Identifier: 159899894

GI number: 159899894

Start: 4256731

End: 4257981

Strand: Reverse

Name: rho [H]

Synonym: Haur_3377

Alternate gene names: 159899894

Gene position: 4257981-4256731 (Counterclockwise)

Preceding gene: 159899895

Following gene: 159899893

Centisome position: 67.09

GC content: 50.84

Gene sequence:

>1251_bases
GTGGTCAACCTTGCTGATTTAGAAAACAAAACGTTGAGCGACCTCCAAGAAATGGCTCGGGAACTCGATATCTCTGGCTA
TAGTCGCCTCAAGAAACAAGACCTCATTTACAAATTAATTCAGGCTCAAACTGAACAAGCAGGCAATATTTTCAATACGG
GGATTCTCGATATCGTTTCTGACGGTTTCGGGTTTCTGCGCAGCGACCGTATGTTGCCTGGCCCCGATGATGTGTATGTC
TCGCAAACTCAAATTCGCCGCTTTGGCCTCCGTACTGGCGACCGCATCTCCGGCCAGATTCGTCCTCCCAAAGAAAGTGA
ACGCTATTATAGTTTGCTGCGGGTTGAATTAGTGAATGGTATGGACCCTGAGCAAGCTCGTAAGCGCCCCCATTTTGAAA
AATTGACCCCGATTTTTCCCAATGAACGCTTTATTTTGGAGACTGAGCCGCAAATTCTTTCAACTCGTTTGGTTGATTTG
ATTGCGCCAATCGGTCGCGGTCAGCGTGGGCTGCTGGTTTCGCCCCCCAAAGCTGGTAAAACGATGCTGATGAAGGCGAT
TGCTAATGGCATCACCACCAATTATCAAGATGCCCATTTGATGGTCTTGTTGATTGGCGAGCGTCCCGAAGAAGTCACCG
ATATGCGCCGCTCAGTCCGCGGTGAGGTGATTGCTTCGACCTTCGATGAGCCAGTTGAAGATCATACCAAAGTTTCTGAA
ATGACCCTCGAACGAGCTAAGCGCTTGGTCGAAGGCGGCCAAGATGTGGTGATCTTGATGGACTCTATCACCCGTTTGGC
ACGGGCTTACAACTTGGATATGCCACCATCAGGCCGAACCTTGACTGGTGGGATCGACCCAGTGGCCTTGTACCCTCCAA
AACGCTTCTTTGGTGCTGCCCGTAACATCGAAGGCGGCGGCTCGTTGACAATCATTGCTACCTGTTTGGTCGATACTGGT
AGCCGTATGGACGACGTGATTTACGAAGAATTCAAGGGTACTGGCAACATGGAATTGCACTTGGATCGGCGCTTGGCTGA
ACGCCGCACCTATCCGGCAGTCGATATTGCCCGCTCTTCAACCCGTCGCGATGAGTTGTTGCTCTTGCCTGAGCAATTGC
GCCAAGTTTGGACGTTGCGCCGTATGGTCAGTATGTTGGGCGAAAACGAAGGCACTGAATTGGTGCTGACGCGCATGTCC
AAAACCCGTACCAACGACGAGTTCTTGCTCACTCTCAACAAGAGCCTCTAG

Upstream 100 bases:

>100_bases
CTGAACTATGCTATTAGTTCGCCACGCAACGCCATTGATTTTTTGAAGAGGTCGGATGATGCACGCCCGCTGTAAGAATT
CATCGTAGGAGGATAGCACG

Downstream 100 bases:

>100_bases
AATTCGATTAATCGATCCCCTCACCCCAACCCCTCTCCCATGCGCGGGCGAGGGGCTTTTTGATCCACGAAGGACACGGA
GCTACACGAAGAATATTTTA

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MVNLADLENKTLSDLQEMARELDISGYSRLKKQDLIYKLIQAQTEQAGNIFNTGILDIVSDGFGFLRSDRMLPGPDDVYV
SQTQIRRFGLRTGDRISGQIRPPKESERYYSLLRVELVNGMDPEQARKRPHFEKLTPIFPNERFILETEPQILSTRLVDL
IAPIGRGQRGLLVSPPKAGKTMLMKAIANGITTNYQDAHLMVLLIGERPEEVTDMRRSVRGEVIASTFDEPVEDHTKVSE
MTLERAKRLVEGGQDVVILMDSITRLARAYNLDMPPSGRTLTGGIDPVALYPPKRFFGAARNIEGGGSLTIIATCLVDTG
SRMDDVIYEEFKGTGNMELHLDRRLAERRTYPAVDIARSSTRRDELLLLPEQLRQVWTLRRMVSMLGENEGTELVLTRMS
KTRTNDEFLLTLNKSL

Sequences:

>Translated_416_residues
MVNLADLENKTLSDLQEMARELDISGYSRLKKQDLIYKLIQAQTEQAGNIFNTGILDIVSDGFGFLRSDRMLPGPDDVYV
SQTQIRRFGLRTGDRISGQIRPPKESERYYSLLRVELVNGMDPEQARKRPHFEKLTPIFPNERFILETEPQILSTRLVDL
IAPIGRGQRGLLVSPPKAGKTMLMKAIANGITTNYQDAHLMVLLIGERPEEVTDMRRSVRGEVIASTFDEPVEDHTKVSE
MTLERAKRLVEGGQDVVILMDSITRLARAYNLDMPPSGRTLTGGIDPVALYPPKRFFGAARNIEGGGSLTIIATCLVDTG
SRMDDVIYEEFKGTGNMELHLDRRLAERRTYPAVDIARSSTRRDELLLLPEQLRQVWTLRRMVSMLGENEGTELVLTRMS
KTRTNDEFLLTLNKSL
>Mature_416_residues
MVNLADLENKTLSDLQEMARELDISGYSRLKKQDLIYKLIQAQTEQAGNIFNTGILDIVSDGFGFLRSDRMLPGPDDVYV
SQTQIRRFGLRTGDRISGQIRPPKESERYYSLLRVELVNGMDPEQARKRPHFEKLTPIFPNERFILETEPQILSTRLVDL
IAPIGRGQRGLLVSPPKAGKTMLMKAIANGITTNYQDAHLMVLLIGERPEEVTDMRRSVRGEVIASTFDEPVEDHTKVSE
MTLERAKRLVEGGQDVVILMDSITRLARAYNLDMPPSGRTLTGGIDPVALYPPKRFFGAARNIEGGGSLTIIATCLVDTG
SRMDDVIYEEFKGTGNMELHLDRRLAERRTYPAVDIARSSTRRDELLLLPEQLRQVWTLRRMVSMLGENEGTELVLTRMS
KTRTNDEFLLTLNKSL

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=419, Percent_Identity=55.3699284009546, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI1788251, Length=278, Percent_Identity=28.0575539568345, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 46996; Mature: 46996

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNLADLENKTLSDLQEMARELDISGYSRLKKQDLIYKLIQAQTEQAGNIFNTGILDIVS
CCCCHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHHHH
DGFGFLRSDRMLPGPDDVYVSQTQIRRFGLRTGDRISGQIRPPKESERYYSLLRVELVNG
CCHHHHHCCCCCCCCCCCEECHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
MDPEQARKRPHFEKLTPIFPNERFILETEPQILSTRLVDLIAPIGRGQRGLLVSPPKAGK
CCHHHHHCCCCHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCH
TMLMKAIANGITTNYQDAHLMVLLIGERPEEVTDMRRSVRGEVIASTFDEPVEDHTKVSE
HHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
MTLERAKRLVEGGQDVVILMDSITRLARAYNLDMPPSGRTLTGGIDPVALYPPKRFFGAA
HHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEECCCHHHHHHH
RNIEGGGSLTIIATCLVDTGSRMDDVIYEEFKGTGNMELHLDRRLAERRTYPAVDIARSS
CCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCHHHHHCC
TRRDELLLLPEQLRQVWTLRRMVSMLGENEGTELVLTRMSKTRTNDEFLLTLNKSL
CCCCCEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCCCCCEEEEEEECCC
>Mature Secondary Structure
MVNLADLENKTLSDLQEMARELDISGYSRLKKQDLIYKLIQAQTEQAGNIFNTGILDIVS
CCCCHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHHHH
DGFGFLRSDRMLPGPDDVYVSQTQIRRFGLRTGDRISGQIRPPKESERYYSLLRVELVNG
CCHHHHHCCCCCCCCCCCEECHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
MDPEQARKRPHFEKLTPIFPNERFILETEPQILSTRLVDLIAPIGRGQRGLLVSPPKAGK
CCHHHHHCCCCHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCH
TMLMKAIANGITTNYQDAHLMVLLIGERPEEVTDMRRSVRGEVIASTFDEPVEDHTKVSE
HHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
MTLERAKRLVEGGQDVVILMDSITRLARAYNLDMPPSGRTLTGGIDPVALYPPKRFFGAA
HHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEECCCHHHHHHH
RNIEGGGSLTIIATCLVDTGSRMDDVIYEEFKGTGNMELHLDRRLAERRTYPAVDIARSS
CCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCHHHHHCC
TRRDELLLLPEQLRQVWTLRRMVSMLGENEGTELVLTRMSKTRTNDEFLLTLNKSL
CCCCCEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423140; 9353933; 9384377 [H]