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The map label for this gene is aquIMA [H]

Identifier: 159899163

GI number: 159899163

Start: 3391775

End: 3392863

Strand: Direct

Name: aquIMA [H]

Synonym: Haur_2644

Alternate gene names: 159899163

Gene position: 3391775-3392863 (Clockwise)

Preceding gene: 159899162

Following gene: 159899164

Centisome position: 53.44

GC content: 37.65

Gene sequence:

>1089_bases
ATGTCAAAAATTACGAATCACTATACACGTAATGTGGGGATGCAAGTTCAAGAGCGGATTAATCATCTTGGTATTGGGCA
AAAAATGCAGGATCTACCCGAACACCTTTGGCATGAAAGTTTTCGCTATTATGTTAAAGAAGATCCATCACGTCAAGGTG
GGCCAAATCTACGGATGATTCGCTTAGATCCTAATAAGCCATCGTTAACAGTTACTGCCTTTATCTTCAATAAATTTGTC
CATCCTTTTGAAAATAGATTTATTACAACTCGTGAGGCTGCACGATTACAAGGCTTTCCTGACAACTTCGAATTTATTGG
CTCATTAACTAGTATTCAACGTCAAATCGGTAATGCAGTTCCAGTTCCATTAGCAACGGCTATATTACAAACAATCTTAC
AGCATGCAAAAGCTCATCATCCAGAAAAAAGCTTATTTTCAGCATTAAGTATTTTCAGTGGGGCAGGTGGGCTTAATTTA
GGTGCTCAACAGGCCAAATTACCCAATGCAAAATGGCAAACAATTGCATCAATTGATATCGATCGTGATGCATGTACCAG
TCTTGAACATCATTTCGCGAATAAAAATGTGATTTGTCAAAATATTATTGATATTACTCAACCTAAATCATTAATGAGCC
AACCACTCGATCTATCATATGGTGGGCCTCCTTGTCAATCATTCAGTCAGGCTGGTAAACAAAAAGGGTTATCCGATCCT
CGTGGAAATTTAATTTACGAGTTTATTCGTTTTCTAAGTGATCTTAATCCAAATTACTTTTTGTTAGAGAACGTAAAAGG
TTTACAGGGAATTAATAATGGCCAATTATTACATCTGATAATCGATGATATTCGTAAGCTTGGGTATAACGTAACATTCG
GTGTAGTCAATGCTGCTGATTATGGAACACCGCAGCTTCGCAAAAGAATTATTATCCTAGGTTGTAAGCAAGATTTAGGA
TTTGTAAATCTGCCGCTACCAACGCATGCGACTGAGGCTAATTTGCTATTACAGCCCTATAAAACAGTTGGTCAAGCTTT
GCAAAATCTACCACCCGCCTTAGTTTGGCAAAAAACAACCACCAAGTAA

Upstream 100 bases:

>100_bases
GCCAACTTTATCTGCGACGTGATTGCCGAGCTGCTTGGATCATTCCTCAATCTGATTAAGAGCTAAAACAGAATCGAGTT
CAATTCTAGGACTATATGTT

Downstream 100 bases:

>100_bases
CGAACTACTTGGTGGTTGTTCTAAATTGCGTTTGATAATTCAAACCCATATAGCATCGCCAAAATCGCATTAATCTCATC
GCCCTCACCGTAAACTCGCT

Product: C-5 cytosine-specific DNA methylase

Products: NA

Alternate protein names: M.AquI subunit alpha; M.AquiA; Cytosine-specific methyltransferase AquI subunit alpha [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MSKITNHYTRNVGMQVQERINHLGIGQKMQDLPEHLWHESFRYYVKEDPSRQGGPNLRMIRLDPNKPSLTVTAFIFNKFV
HPFENRFITTREAARLQGFPDNFEFIGSLTSIQRQIGNAVPVPLATAILQTILQHAKAHHPEKSLFSALSIFSGAGGLNL
GAQQAKLPNAKWQTIASIDIDRDACTSLEHHFANKNVICQNIIDITQPKSLMSQPLDLSYGGPPCQSFSQAGKQKGLSDP
RGNLIYEFIRFLSDLNPNYFLLENVKGLQGINNGQLLHLIIDDIRKLGYNVTFGVVNAADYGTPQLRKRIIILGCKQDLG
FVNLPLPTHATEANLLLQPYKTVGQALQNLPPALVWQKTTTK

Sequences:

>Translated_362_residues
MSKITNHYTRNVGMQVQERINHLGIGQKMQDLPEHLWHESFRYYVKEDPSRQGGPNLRMIRLDPNKPSLTVTAFIFNKFV
HPFENRFITTREAARLQGFPDNFEFIGSLTSIQRQIGNAVPVPLATAILQTILQHAKAHHPEKSLFSALSIFSGAGGLNL
GAQQAKLPNAKWQTIASIDIDRDACTSLEHHFANKNVICQNIIDITQPKSLMSQPLDLSYGGPPCQSFSQAGKQKGLSDP
RGNLIYEFIRFLSDLNPNYFLLENVKGLQGINNGQLLHLIIDDIRKLGYNVTFGVVNAADYGTPQLRKRIIILGCKQDLG
FVNLPLPTHATEANLLLQPYKTVGQALQNLPPALVWQKTTTK
>Mature_361_residues
SKITNHYTRNVGMQVQERINHLGIGQKMQDLPEHLWHESFRYYVKEDPSRQGGPNLRMIRLDPNKPSLTVTAFIFNKFVH
PFENRFITTREAARLQGFPDNFEFIGSLTSIQRQIGNAVPVPLATAILQTILQHAKAHHPEKSLFSALSIFSGAGGLNLG
AQQAKLPNAKWQTIASIDIDRDACTSLEHHFANKNVICQNIIDITQPKSLMSQPLDLSYGGPPCQSFSQAGKQKGLSDPR
GNLIYEFIRFLSDLNPNYFLLENVKGLQGINNGQLLHLIIDDIRKLGYNVTFGVVNAADYGTPQLRKRIIILGCKQDLGF
VNLPLPTHATEANLLLQPYKTVGQALQNLPPALVWQKTTTK

Specific function: This methylase recognizes the double-stranded sequence CYCGRG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the AquI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4503351, Length=195, Percent_Identity=27.6923076923077, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI195927037, Length=195, Percent_Identity=27.6923076923077, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI4758184, Length=185, Percent_Identity=27.5675675675676, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1788271, Length=227, Percent_Identity=29.0748898678414, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 40496; Mature: 40365

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKITNHYTRNVGMQVQERINHLGIGQKMQDLPEHLWHESFRYYVKEDPSRQGGPNLRMI
CCCHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
RLDPNKPSLTVTAFIFNKFVHPFENRFITTREAARLQGFPDNFEFIGSLTSIQRQIGNAV
EECCCCCCEEEHHHHHHHHHHHHHCCEECCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
PVPLATAILQTILQHAKAHHPEKSLFSALSIFSGAGGLNLGAQQAKLPNAKWQTIASIDI
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCEEEEEEEC
DRDACTSLEHHFANKNVICQNIIDITQPKSLMSQPLDLSYGGPPCQSFSQAGKQKGLSDP
CHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCC
RGNLIYEFIRFLSDLNPNYFLLENVKGLQGINNGQLLHLIIDDIRKLGYNVTFGVVNAAD
CHHHHHHHHHHHHHCCCCEEEEECCCHHCCCCCCCEEHHHHHHHHHCCCCEEEEEEECCC
YGTPQLRKRIIILGCKQDLGFVNLPLPTHATEANLLLQPYKTVGQALQNLPPALVWQKTT
CCCHHHCCEEEEEECCCCCCEEECCCCCCCCCCHHHCCCHHHHHHHHHCCCCCEEECCCC
TK
CC
>Mature Secondary Structure 
SKITNHYTRNVGMQVQERINHLGIGQKMQDLPEHLWHESFRYYVKEDPSRQGGPNLRMI
CCHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
RLDPNKPSLTVTAFIFNKFVHPFENRFITTREAARLQGFPDNFEFIGSLTSIQRQIGNAV
EECCCCCCEEEHHHHHHHHHHHHHCCEECCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
PVPLATAILQTILQHAKAHHPEKSLFSALSIFSGAGGLNLGAQQAKLPNAKWQTIASIDI
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCEEEEEEEC
DRDACTSLEHHFANKNVICQNIIDITQPKSLMSQPLDLSYGGPPCQSFSQAGKQKGLSDP
CHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCC
RGNLIYEFIRFLSDLNPNYFLLENVKGLQGINNGQLLHLIIDDIRKLGYNVTFGVVNAAD
CHHHHHHHHHHHHHCCCCEEEEECCCHHCCCCCCCEEHHHHHHHHHCCCCEEEEEEECCC
YGTPQLRKRIIILGCKQDLGFVNLPLPTHATEANLLLQPYKTVGQALQNLPPALVWQKTT
CCCHHHCCEEEEEECCCCCCEEECCCCCCCCCCHHHCCCHHHHHHHHHCCCCCEEECCCC
TK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2104605 [H]