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The map label for this gene is ycjG [C]

Identifier: 159898550

GI number: 159898550

Start: 2518581

End: 2519606

Strand: Reverse

Name: ycjG [C]

Synonym: Haur_2026

Alternate gene names: 159898550

Gene position: 2519606-2518581 (Counterclockwise)

Preceding gene: 159898551

Following gene: 159898549

Centisome position: 39.7

GC content: 49.71

Gene sequence:

>1026_bases
ATGAACTTAACCACAACAGTGCTCAGCCTGCAACTTGAGCAACCGTTTGTCAGCAATAAGGGCTCGACGACTACGGTGCA
CCAAGTTGTAATCAAATTAACTTGGCAGGAGTATGGTGGTTTTGGTACGGTACTTTGCCCCAAAGAAACCCAACTTAGTG
TTGAGCAAATTCAACAGCTGATTCAGGCTTGTGAACCATTGCTTAGTACCGCCACACCATGGCAATTTGAACTGTATCAA
GGTCAATTAGCCTCAGTCGTTCGCAATCAGGCTGCAATGATGGCTGGCATCGATATGGCATGGCATGATCTTTTGGGCAA
GGTGGTTGCCCAACCCATCCACGCGCTTTGGGGCTTGGCAGGGTTGAGCATCCCACCAACGGCACTCTCGCTTGGCGCAC
AATCGGAGCAGGCCTTGGTCGCACAGGCTGCAAAATTGGCGGCATGGCCAATTCTTAAACTCAAACTCACAACCGATAGC
AATCTCGATAGCCTGCGCCAACTACGCGAGGTCTATGCTGGGCGGATTTGGGTTGATGGCAATGGAGCATGGGATGTTGA
TCAAGCGATTGCTGCGGCGCAACAATGCCATACCTATGGGGTTGAACTGATCGAACAGCCAATTCCAGCGGGCAACCTCG
ACCAACTGCGCACAATTCGCCAACACTCACCAATTCCCATAGTTGCCGATGAAGATTGTCGTGGGCTTGCTGATGTGCTG
CGCTTGCATACATGTGTTGATGTAATTAATCTCAAACTCTTCAAATGTGGAGGCTTACGCCAAGCTCGCACGATGATCGA
CGTGGCCAAGCAATTTGGCTTAAAAGTTATGTTGGGTTGTAAAACTGAAAGCAGCCTTGGAATTAGCGCCATCGCCCAAC
TTGCCGGGCTAGCAGATTACCTTGATCTTGATGGGCATCTTGATTTGGTCAATGACCCCTTTCAAGGCCTTGTGATCGAG
CAAGGTACGCTGCGTTTACCGCAAACTCCAGGTTTAGGATTAACCATTCAAGGAGCAATCGAATGA

Upstream 100 bases:

>100_bases
TGGCGTTGGTGCTTGGCAGTCCGCGATTTTTGGGCTGGCGCTTACTTGGCGAAGAACCTGTCGCCGCAACCACCCCAGCT
GCTTTAGCGGAAACAAGCTC

Downstream 100 bases:

>100_bases
CAAAACTACGCTGTGCAATTCTTGACGATTATCAACACGCCGCCTTGGCGATGGCTGATTGGTCAGTGGTAGCCGAACAG
GTTGAAGTAGAGATTTTTTC

Product: mandelate racemase/muconate lactonizing protein

Products: 3-chloro-cis,cis-muconate

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MNLTTTVLSLQLEQPFVSNKGSTTTVHQVVIKLTWQEYGGFGTVLCPKETQLSVEQIQQLIQACEPLLSTATPWQFELYQ
GQLASVVRNQAAMMAGIDMAWHDLLGKVVAQPIHALWGLAGLSIPPTALSLGAQSEQALVAQAAKLAAWPILKLKLTTDS
NLDSLRQLREVYAGRIWVDGNGAWDVDQAIAAAQQCHTYGVELIEQPIPAGNLDQLRTIRQHSPIPIVADEDCRGLADVL
RLHTCVDVINLKLFKCGGLRQARTMIDVAKQFGLKVMLGCKTESSLGISAIAQLAGLADYLDLDGHLDLVNDPFQGLVIE
QGTLRLPQTPGLGLTIQGAIE

Sequences:

>Translated_341_residues
MNLTTTVLSLQLEQPFVSNKGSTTTVHQVVIKLTWQEYGGFGTVLCPKETQLSVEQIQQLIQACEPLLSTATPWQFELYQ
GQLASVVRNQAAMMAGIDMAWHDLLGKVVAQPIHALWGLAGLSIPPTALSLGAQSEQALVAQAAKLAAWPILKLKLTTDS
NLDSLRQLREVYAGRIWVDGNGAWDVDQAIAAAQQCHTYGVELIEQPIPAGNLDQLRTIRQHSPIPIVADEDCRGLADVL
RLHTCVDVINLKLFKCGGLRQARTMIDVAKQFGLKVMLGCKTESSLGISAIAQLAGLADYLDLDGHLDLVNDPFQGLVIE
QGTLRLPQTPGLGLTIQGAIE
>Mature_341_residues
MNLTTTVLSLQLEQPFVSNKGSTTTVHQVVIKLTWQEYGGFGTVLCPKETQLSVEQIQQLIQACEPLLSTATPWQFELYQ
GQLASVVRNQAAMMAGIDMAWHDLLGKVVAQPIHALWGLAGLSIPPTALSLGAQSEQALVAQAAKLAAWPILKLKLTTDS
NLDSLRQLREVYAGRIWVDGNGAWDVDQAIAAAQQCHTYGVELIEQPIPAGNLDQLRTIRQHSPIPIVADEDCRGLADVL
RLHTCVDVINLKLFKCGGLRQARTMIDVAKQFGLKVMLGCKTESSLGISAIAQLAGLADYLDLDGHLDLVNDPFQGLVIE
QGTLRLPQTPGLGLTIQGAIE

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the epimerization of L-Ala-D-Asp [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=324, Percent_Identity=29.0123456790123, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 5.5.1.7

Molecular weight: Translated: 36925; Mature: 36925

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTTTVLSLQLEQPFVSNKGSTTTVHQVVIKLTWQEYGGFGTVLCPKETQLSVEQIQQL
CCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHH
IQACEPLLSTATPWQFELYQGQLASVVRNQAAMMAGIDMAWHDLLGKVVAQPIHALWGLA
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLSIPPTALSLGAQSEQALVAQAAKLAAWPILKLKLTTDSNLDSLRQLREVYAGRIWVDG
CCCCCCHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHCCEEEECC
NGAWDVDQAIAAAQQCHTYGVELIEQPIPAGNLDQLRTIRQHSPIPIVADEDCRGLADVL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHH
RLHTCVDVINLKLFKCGGLRQARTMIDVAKQFGLKVMLGCKTESSLGISAIAQLAGLADY
HHHHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHH
LDLDGHLDLVNDPFQGLVIEQGTLRLPQTPGLGLTIQGAIE
HCCCCCEEECCCCCCCEEEECCCEECCCCCCCCEEEEECCC
>Mature Secondary Structure
MNLTTTVLSLQLEQPFVSNKGSTTTVHQVVIKLTWQEYGGFGTVLCPKETQLSVEQIQQL
CCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHH
IQACEPLLSTATPWQFELYQGQLASVVRNQAAMMAGIDMAWHDLLGKVVAQPIHALWGLA
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLSIPPTALSLGAQSEQALVAQAAKLAAWPILKLKLTTDSNLDSLRQLREVYAGRIWVDG
CCCCCCHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHCCEEEECC
NGAWDVDQAIAAAQQCHTYGVELIEQPIPAGNLDQLRTIRQHSPIPIVADEDCRGLADVL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHH
RLHTCVDVINLKLFKCGGLRQARTMIDVAKQFGLKVMLGCKTESSLGISAIAQLAGLADY
HHHHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHH
LDLDGHLDLVNDPFQGLVIEQGTLRLPQTPGLGLTIQGAIE
HCCCCCEEECCCCCCCEEEECCCEECCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-chloro-2,5-dihydro-5-oxofuran-2-acetate

Specific reaction: 2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]